• Journal of Microbiology and Biotechnology
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• v.26 no.3
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• pp.511-520
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• 2016

The most energy-demanding step of wastewater treatment is the aeration-dependent elimination of organic carbon. Microbial fuel cells (MFCs) offer an alternative strategy in which carbon elimination is conducted by anaerobic microorganisms that transport respiratory electrons originating from carbon oxidation to an anode. Hence, chemical energy is directly transformed into electrical energy. In this study, the use and stability of barcode-containing exoelectrogenic model biofilms under non-axenic wastewater treatment conditions are described. Genomic barcodes were integrated in Shewanella oneidensis, Geobacter sulfurreducens, and G. metallireducens. These barcodes are unique for each strain and allow distinction between those cells and naturally occurring wild types as well as quantification of the amount of cells in a biofilm via multiplex qPCR. MFCs were pre-incubated with these three strains, and after 6 days the anodes were transferred into MFCs containing synthetic wastewater with 1% wastewater sludge. Over time, the system stabilized and the coulomb efficiency was constant. Overall, the initial synthetic biofilm community represented half of the anodic population at the end of the experimental timeline. The part of the community that contained a barcode was dominated by G. sulfurreducens cells (61.5%), while S. oneidensis and G. metallireducens cells comprised 10.5% and 17.9%, respectively. To the best of our knowledge, this is the first study to describe the stability of a synthetic exoelectrogenic consortium under non-axenic conditions. The observed stability offers new possibilities for the application of synthetic biofilms and synthetically engineered organisms fed with non-sterile waste streams.

• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.305-311
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• 2002

Acetyl xylan esterase gene (AXE) from Aspergillus ficuum was cloned and its Pichia expression plasmid, pPICZ$\alpha$C-AXE (4.6 kb), was constructed, in which the AXE gene was under the control of the AOXI promoter and connected downstream of mating factor u-1 signal sequence. The plasmid linearized by Sacl was integrated into the 5'AOXI region of the chromosomal DNA of P. pastoris. In the flask batch culture of P. pastoris transformant on methanol medium, the cell concentration and total AXEase activity reached at 6.0 g-dry cell weight/1 and 77 unit/ml after 36 h cultivation, respectively. In the fed-batch culture employing the optimized methanol and histidine feeding strategy, the cell concentration and total AXEase activity were significantly increased to about 97 g-dry cell weight/1 and 930 unit/ml. Most of AXEase activity (>90%) was found in the extracellular medium and the majority of extracellular protein (>80%) was AXEase enzyme (33.5 kDa). This result means that about 9.8 g/1 of AXEase protein was produced in the extracellular medium.

• Environmental Engineering Research
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• v.15 no.2
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• pp.105-109
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• 2010

The European Union (EU) Water Framework Directive (WFD) (2000/60/EC) was transposed into Irish law by Statutory Instrument Nos. 722 of 2003, 413 of 2005 and 218 of 2009, which set out a new strategy and process to protect and enhance Ireland's water resources and water-dependent ecosystems. The Directive requires a novel, holistic, integrated, and iterative process to address Ireland's natural waters based on a series of six-year planning cycles. Key success factors in implementing the Directive include an in-depth and balanced treatment of the ecological, economic, institutional and cultural aspects of river basin management planning. Introducing this visionary discipline for the management of sustainable water resources requires a solemn commitment to a new mindset and an overarching monitoring and management regime which hitherto has never been attempted in Ireland. The WFD must be implemented in conjunction with a myriad of complimentary directives and associated legislation, addressing such key related topics as flood/drought management, biodiversity protection, land use planning, and water/wastewater and diffuse pollution engineering and regulation. The critical steps identified for river basin management planning under the WFD include: 1) characterization and classification of water bodies (i.e., how healthy are Irish waters?), 2) definition of significant water pressures (e.g., agriculture, forestry, septic tanks), 3) enhancement of measures for designated protected areas, 4) establishment of objectives for all surface and ground waters, and 5) integrating these critical steps into a comprehensive and coherent river basin management plan and associated programme of measures. A parallel WFD implementation programme critically depends on an effective environmental management system (EMS) approach with a plan-do-check-act cycle applied to each of the evolving six-year plans. The proactive involvement of stakeholders and the general public is a key element of this EMS approach.

• The Korean Journal of Applied Statistics
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• v.7 no.1
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• pp.83-102
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• 1994

Some optimal designs and data analysis methods based on a Gaussian spatial linear model for computer simulation experiments are considered. For designs of computer experiments, Latin-hypercube designs and some optimal designs are combined. A two-stage computational (2-points exchange and Newton-type) algorithm for finding the optimal Latin-hypercube design is presented. The spatial prediction model which was discussed by Sacks, Welch, Mitchell and Wynn(1989) for computer experiments, is used for analysis of the simulated data. Moreover, a method of contructing sequential (optimal) Latin-hypercube designs is considered. An application of this approach to the quality improvement and optimization of the integrated circuit design via the main-effects plot and the sequential experimental strategy is presented.

• Journal of Korea Water Resources Association
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• v.42 no.10
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• pp.809-824
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• 2009

Hydrological system forecasting, which is the short term runoff historical data during the limited period in dam site, is a conditional precedent of hydrological persistence by stochastic analysis. We have forecasted the monthly hydrological system from Andong dam basin data that is the rainfall, evaporation, and runoff, using the seasonal ARIMA (autoregressive integrated moving average) model. Also we have conducted long term runoff simulations through the forecasted results of TANK model and ARIMA+TANK model. The results of analysis have been concurred to the observation data, and it has been considered for application to possibility on the stochastic model for dam inflow forecasting. Thus, the method presented in this study suggests a help to water resource mid- and long-term strategy establishment to application for runoff simulations through the forecasting variables of hydrological time series on the relatively short holding runoff data in an object basins.

• International Journal of Aeronautical and Space Sciences
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• v.10 no.2
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• pp.134-139
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• 2009

Various modules are generally combined with one another in order to perform rotorcraft preliminary design and its optimization. At the stage of the preliminary design, analysis fidelity is less important than the rapid assessment of a design is. Most of the previous researchers attempted to implement sophisticated applications in order to increase the fidelity of analysis, but the present paper focuses on a rapid assessment while keeping the similar level of fidelity. Each small-sized module will be controlled by an externally-operated global optimization module. Results from each module are automatically handled from one discipline to another which reduces the amount of computational effort and time greatly when compared with manual execution. Automatically handled process decreases computational cycle and time by factor of approximately two. Previous researchers and the rotorcraft industries developed their own integrated analysis for rotorcraft design task, such as HESCOMP, VASCOMP, and RWSIZE. When a specific mission profile is given to these programs, those will estimate the aircraft size, performance, rotor performance, component weight, and other aspects. Such results can become good sources for the supplemental analysis in terms of stability, handling qualities, and cost. If the results do not satisfy the stability criteria or other constraints, additional sizing processes may be used to re-evaluate rotorcraft size based on the result from stability analysis. Trade-off study can be conducted by connecting disciplines, and it is an important advantage in a preliminary design study. In this paper among the existing rotorcraft design programs, an adequate program is selected for a baseline of the design framework, and modularization strategy will be applied and further improvements for each module be pursued.

• The Journal of Information Systems
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• v.13 no.1
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• pp.77-95
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• 2004

According to the increase of organizational complexity and the change of rapid information technology environment, many firms have shifted their information technology(IT) strategy from developing information systems in-house to purchasing application software such as Enterprise Resource Planning(ERP) Systems. Marketing and Sales functions within a Korean automotive part company include developing new assembly products, determining pricing, taking customer's orders, and shipping assembly products to customers. Marketing and Sales Information System(MSIS) in ERP system plays an important role in next Production Planning process. MSIS also makes management reporting and decision making faster and more uniform throughout an organization. MSIS promotes thinking about corporate goals, as opposed to thinking only about the goals of a single department or functional area. This paper intends to design and implement a MSIS in ERP systems for Korean automotive part company using object-oriented methodology In order to accomplish the implementation of MSIS in ERP system, we employed UML as its standard modeling language. In this study, four diagramming techniques such as use case diagram, sequence diagram, class diagram, component diagram among eight modeling techniques are used for analyzing hierarchical business process. In traditional marketing and sales function, a company with an unintegrated information system can have marketing and sales data that is data redundant or inaccurate. MSIS integrated in ERP system can solve the sales forecast problem, which minimizes the total costs of production, inventory, and transportation under constraints of production capacity. Also, the use of UML methodology makes S/W programmers shorten the phase of analysis and design in the implementation of MSIS system, and increase the reuse of software and the interoperability with corporate internal Information system.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.563-574
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• 2008

Most of earthquake-induced geotechnical hazards have been caused by the site effects relating to the amplification of ground motion, which are strongly influenced by the local geologic conditions such as soil thickness or bedrock depth and soil stiffness. In this study, an integrated GIS-based information system for geotechnical data, called geotechnical information system (GTIS), was constructed to establish a regional counterplan against earthquake-induced hazards at an urban area, Daejeon, which is represented as a hub of research and development in Korea. To build the GTIS for the area of interesting, pre-existing geotechnical data collections were performed across the extended area including the study area and a walk-over site survey was additionally carried out to acquire surface geo-knowledge data. For practical application of the GTIS used to estimate the site effects at the area of interesting, seismic microzoning map of the characteristic site period was created and presented as regional synthetic strategy for earthquake-induced hazards prediction. In addition, seismic zonation for site classification according to the spatial distribution of the site period was also performed to determine the site amplification coefficients for seismic design and seismic performance evaluation at any site in the study area. Based on the case study on seismic zonations at Daejeon, it was verified that the GIS-based GTIS was very useful for the regional prediction of seismic hazards and also the decision support for seismic hazard mitigation.

• 한국정보디스플레이학회:학술대회논문집
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• 2008.10a
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• pp.1441-1444
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• 2008

We developed ultra-accelerated quantum chemical molecular dynamics and spectroscopic characterization simulators for development of PDP materials. By combination of these simulators, realistic structure of PDP materials is drawn on the computer. Furthermore, based on the structures, various properties such as cathode luminescence spectrum and secondary electron emission, is successfully evaluated. The strategy of "Experiment integrated Computational Chemistry" using developed simulators will presented that has the potential in being powerful tool for designing the PDP materials.

• Journal of Korean Elementary Science Education
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• v.24 no.3
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• pp.214-225
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• 2005

The purpose of this study was to examine the content relevance of the 7th national primary science curriculum and textbooks. To fulfill the purpose of the study, we 1) analyzed the 7th national science curriculum of Korea, Californian science standards, the national science curriculum of England and Japan, and current Korean and Japanese science textbooks, 2) conducted a nationwide survey to gather opinions from students, teachers, and professors of teachers colleges about the relevance of the science curriculum and textbooks. Main findings of this study are as follows: First, the science contents at each grade level should be determined by the students' characteristics, not by an equal portion rule among physics, chemistry, biology and earth science. Second, the excessive overlapping and repetition of contents due to the spiral curriculum should be avoid. Third, the number of topics at each grade level should be reduced, and the similar topics and themes should be integrated for students' deeper understanding. Fourth, the number of science concepts and activities should be reduced to an appropriate level considering the time allotment for science classes, teachers workload, laboratory conditions, etc. Fifth, differentiated curriculum, such as in-depth and supplementary course, should be described not in science contents, but in teaching and learning strategy.