• Korean Journal of Poultry Science
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• v.40 no.1
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• pp.31-40
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• 2013

This study was performed to investigate the investigated effects of dietary supplementation of vitamin C and E on the growth performance and stress response in broiler chickens. Stress response was analyzed by the quantity of telomeric DNA, the rate of DNA damage and the expression levels of heat shock proteins (HSPs) and hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) genes on tissues and blood. The telomere length and telomere shortening rates were analyzed by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes and tissues. The DNA damage rate of lymphocytes was quantified by the comet assay. The expression levels of HSP70, HSP90s and HMGCR genes were measured by quantitative real-time polymerase chain reaction in lymphocytes. In results, there was no significant difference among treatments in body weight, weight gain, feed intake and mortality. The telomere shortening rate of the lymphocytes was significantly lower in the vitamin E supplemented group than the control group. The DNA damage was also decreased supplemented with vitamin C and E, as compared to the control group. The vitamin E supplemented group had a significant positive effect on the expressions of HMGCR, HSP90-${\alpha}$ and HSP90-${\beta}$ in lymphocytes, but had no significance on HSP70, as compared to the control group. We concluded that the dietary supplementation of vitamin E (100 mg/kg feed) had reduced the individual physiological stress response without stunt growth in broiler chickens.

• Journal of the East Asian Society of Dietary Life
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• v.25 no.3
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• pp.405-416
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• 2015

This study aimed to compare the dietary habits, nutritional knowledge, and health-related behaviors of students by high school type using questionnaires. The subjects were 586 male students (academic high school students (AS); 294, specialized high school students (SS); 292) in Jinju, Gyeongnam. The frequencies of eating breakfast and snacks were higher in AS than in SS (p<0.05), whereas the frequency of drinking was higher in SS than in AS (p<0.05). The main reason for skipping breakfast was 'poor appetite' in AS (45.2%) and 'lack of time' in SS (56.5%) (p<0.05). Favorite snacks included 'pizza, hamburger' (33.0%) and 'bread, noddle, ramyun' (31.0%) in AS as well as 'pizza, hamburger' (32.5%) and 'fruit, fruit juice' (26.0%) in SS. A mean of 56.2% of students ate an unbalanced diet, and the main reason was 'untasty' (47.2%). The frequency of eating out was higher in SS than in AS (p<0.05), and the main menu while eating out was 'Korean food' in AS (96.3%) and SS (90.3%). The frequency of 'meat, fish, egg, beans' was higher in AS than in SS (p<0.05). On the other hand, the frequencies of 'fruit, fruit juices' (p<0.05), 'milk, milk products' (p<0.01), 'seaweeds' (p<0.05), 'instant foods' (p<0.001) and 'soda and ion drinks' (p<0.001) were higher in SS than in AS. The mean rates of drinking and smoking in students were 84% and 29.5%, respectively. The main reason for drinking and smoking was 'to relieve stress' in AS (38.0%) and SS (30.9%) (p< 0.001). In general, the nutritional knowledge level of SS was higher than that of AS. In conclusion, there were significant differences in the frequencies of eating breakfast, food intake, drinking and reasons for eating snacks, unbalanced diet, drinking and smoking between AS and SS. Therefore, it's necessary to provide proper nutritional education for students according to high school type.

• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.265-271
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• 2016

Recently, liver damage contributes to big percentage of the morbidity and mortality rates worldwide. Excessive intake of alcohol is one of the major causes of liver injury. When liver injury is repeated and becomes chronic, it leads to development of fibrosis and cirrhosis. In the liver, TGF-${\beta}$ is a profibrogenic cytokine, which participates in various critical events cause liver fibrosis. Seahorse (Hippocampus abdominalis) is a common traditional Chinese medicine and has been widely used for centuries. Seahorse has been known to have a variety of bioactivities, such as anti-oxidant, anti-fatigue, and anti-tumor. Peptide is one of the main compounds of seahorse. In this study, we isolated enzymatic hydrolysate from seahorse H. abdominalis by alcalase hydrolysis and investigated the effect of the hydrolysate on liver injury. In the present in vitro studies, the hydrolysate increases cell viability of Chang cells and protects Huh7 cells from ethanol toxicity. In addition, the hydrolysate inhibits TGF-${\beta}$-induced responses. In vivo studies show that the pretreatment of hydrolysate reduces alcohol-induced increases of serum Glutamic oxaloacetic acid transaminase and Glutamic pyruvate transaminase activities and increases liver weight and body weight. These results suggest that seahorse may have a hepatoprotective effect.

• Korean Journal of Environmental Agriculture
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• v.19 no.4
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• pp.332-338
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• 2000

This experiment was conducted to investigate the effects of feeding with MS (miraculous soil-bacteria)-fermented food waste on feeding efficiency and meat quality of mallard. Twenty one heads of 12 weeks-old mallards were fed with three different mixing rates of MS-fermented food waste (0, 20 and 50 %) with 7 replicates. After 6 weeks of feeding, weight gain and feed intake were measured to estimate feeding efficiency, moreover physico-chemical characteristics, amino acids and fatty acids of the fresh slaughtered mallards were analyzed to determine meat quality. The feeding efficiency of 0 % fermented food waste (control) was significantly (p<0.05) higher than other treatments. Water holding capacity and pH were not significantly differed. The color value in lightness of control meat was higher, while in redness was lower than that of the plots mixed with fermented food waste. Total content of amino acids in thigh meat was relatively lower in control, but that in breast meat showed a reverse trend. The amount of unsaturated fatty acids in both thigh and breast meat was the highest in the 50 % mixed plot. Considering economic and environmental aspects for mallard feeding, this results indicated that MS fermented food waste could be a useful resources for mallard feed.

• Journal of Animal Reproduction and Biotechnology
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• v.34 no.3
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• pp.173-182
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• 2019

This study was performed to evaluate the effect of heat stress on the status of physiological responses, blood parameter, serum T3 and cortisol, and heat shock proteins (HSP 27, 70, and 90) of Hanwoo cattle. Six Hanwoo steers (242.8 ± 7.2 kg of BW) were housed in the climate-controlled respiration chambers. The experiment consisted of 7 days (control; 0 day) at thermoneutral (air temperature (Ta) of 15℃ and relative humidity (RH) of 60%; temperature-humidity index (THI) = 64), and by 3 and 6 days (treatment groups) at heat stress (Ta of 35℃ and RH of 60%; THI = 87). Body temperature of each parts (frank, rump, perineum and foot) and rectal temperature elevated in heat stress groups (3 days and 6 days) than the control group (0 day). Respiration rates increased in 3 days and 6 days (88.5 ± 0.96 bpm and 86.3 ± 0.63 bpm, respectively) from 0 days (39.5 ± 0.65 bpm). Feed intake significantly decreased in heat stress groups (3 days and 6 days, 3.7 ± 0.14 kg and 4.0 ± 0.15 kg, respectively) than the control group (0 day, 5.0 ± 0.00 kg). In addition, final BW significantly decreased in heat stress groups (3 days and 6 days, 211.8 ± 4.75 kg and 215.5 ± 3.50 kg, respectively) than the control group (0 day, 240.0 ± 25.00 kg). However, heat stress has no significant effect on blood parameter, serum T3 and cortisol. Nevertheless, heat stress increased HSPs mRNA expression in liver tissue, and serum concentration of HSPs. Despite Hanwoo cattle may have high adaptive ability to heat stress, our results suggested that heat stress directly effect on body temperature and respiration rate as well as serum and tissue HSPs. Therefore, we are recommended that HSPs could be the most appropriate indicators of Hanwoo cattle response to heat stress.

• Journal of Animal Environmental Science
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• v.12 no.2
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• pp.51-60
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• 2006

Studies were carried out using growing to finishing pigs to investigate adequate floor space ($m^2/pig$) in Jeju environment. A randomized block design was used to compare the effect of pig performance on 3 stocking rates (low $0.56 m^2/pig$, medium 0.70 and high 0.90). Liveweight gain and feed intake were measured over a 4 week(Growth stage 1 and Growth stage 2) period or a 3 week(Growth stage 3) period. In growth stage 1 (43 to 65 kg body weight ; BW), daily weight gain was significantly higher in the medium stocking rate than in low or high (P<0.05). However, no significant difference in daily weight gain was found among treatments in growth stage 2(65 to 91 kg) or 3 (91 to 105 kg). Dressing percentage, back fat thickness and carcass grade were also not different (P>0.05). In conclusion, daily weight gain of pigs reared in medium density appeared to be higher than in the other groups during the early growing period which is $0.70m^2/pig$ and similar to that recommended by National Livestock Research Institute, RDA.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.9
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• pp.1268-1273
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• 2011

The present study was conducted to determine a feasible method of protein concentrate extraction from rice bran (RBPC) and its effect as a substitution for skim milk in early weaning pig diets. An investigation to extract protein concentrate from full fat rice bran was undertaken to determine the best ratio of water and rice bran, the amount of NaOH and a HCl solvent to use in a simple paddle-type mixer with modified spinning to produce RBPC. The results stated that the best ratio for water mixing in the RBPC extraction process was 1:5 with 20 g NaOH and 30 min in a paddle-type mixer at 300 rpm. A mix of 250 ml 0.2 N HCl was optimum for neutralization and protein precipitation. After the fluid was spun out with a washing machine, the sediment was left for 12-14 hours to complete the filtration. One kilogram of rice bran could produce an average of 324.5 gram RBPC and it contained 3.40% ash, 496.48 kcal of GE/100 gram, 1.94% crude fiber, 28.20% ether extract, 7.64% moisture and 16.66% crude protein, respectively. A total of 45 crossbred piglets, weaned at 3 weeks of age were allotted into control diet (A) and dietary treatments formulated with a four different rates of RBPC substitution for skim milk at a percentage of 25 (B), 50 (C), 77 (D) and 100 (E) respectively, in a randomized complete block (RCB) design. All piglets had free access to feed and water until 8 week of age when the experiment ended. Feed intake, average daily gain, growth rate and feed efficiency were not affected by dietary treatments. Blood test parameters after completion of the growth trial indicated normal health. Even though the mean of cell hemoglobin concentration was significantly different between treatments (p<0.05) it was still within the normal range. The cost difference for BW gain of 100% RBPC substituted for skim milk in the weaning diet was approximately 35% lower than that of the control and the relative cost of production was 96.67, 92.85, 70.75 and 64.48% lower for the replacement of 25, 50, 75 and 100% of skim milk respectively. These results implied that this technology is feasible for use by small scale farmers to improve their self-reliance.

• Development and Reproduction
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• v.15 no.2
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• pp.77-85
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• 2011

To investigate the influence of density and feeding frequency (food amount) on growth, cannibal dynamics and survival of puffer Takifugu obscurus (TL $25.0{\pm}0.5$ mm, BW $0.53{\pm}0.03$ g) during early life history, we conducted one experiment composed with 6 density groups set up as 1.43 inds./${\ell}$, 2.14 inds./${\ell}$, 2.86 inds./${\ell}$, 3.57 inds./${\ell}$, 4.29 inds./${\ell}$ and 7.14 inds./${\ell}$. The experiment was triplicately performed in 1 ton FRP tank for 30 days. Daily food intake (DFI), feed efficiency (FE), growth, cannibal frequency, and survival rates were examined. Although not find any significant relation of density to DFI and FE, we found that growth decreased significantly with the increasing of density under 3.57 inds./${\ell}$, but significantly increased with the increasing of density over 4.29 inds./${\ell}$. Cannibal frequency and daily mortality rate was significantly increased with increasing density and decreasing feeding frequency, inducing the most poor survival rate in the highest density group and low feeding frequency. Therefore, we concluded that the density and the feeding frequencies affect sibling cannibal dynamics and survival of puffer stock.

• The Journal of Engineering Geology
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• v.27 no.4
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• pp.383-392
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• 2017

Excessive abstraction of groundwater near streams for agricultural, domestic, or industrial use can significantly reduce the streamflow. Therefore, proper management of water resources must involve careful monitoring of groundwater use near streams. This study investigates the current status of groundwater intake plans in the Anseongcheon watershed, Korea, in order to understand the portion of groundwater wells according to distance from the stream and the distribution of wells near the stream. Among all the wells in the watershed, 20.5% were permitted and 31.3% were declared within 300m from the stream. In particular, among the wells located near streams, 11.4% were permitted and 88.6% were declared among the wells near streams. Therefore, the total amount of groundwater pumping rates from the declared wells is much higher than that from the permitted wells near the stream. Under current guidelines, investigations of the impact of groundwater use near a stream on streamflow depletion should only consider wells permitted by groundwater law. However, prudent management should also pay attention to declared wells, given their large number.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.12
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• pp.1745-1752
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• 2008

This study was conducted to compare the growth performance and carcass evaluation of Jeju native cattle (JNC) and its crossbreds (CBK = 25 JNC: 50 Charolais: 25 Brahman and BCBK = 62.5 JNC: 25 Charolais: 12.5 Brahman) fed for a short fattening period. Eight male calves each of JNC (80.$40{\pm}10$), CBK ($113.50{\pm}12.3$), and BCBK ($100.3{\pm}9.5$) were weaned at 4 month of age and were fed similar diets for 18 months of their age. All animals were fed a growing ration until 12 months of age and thereafter switched to a fattening ration for a period of 6 months. Final body weight (BW) and BW gain were significantly higher in CBK and BCBK compared with JNC. The CBK and BCBK gained 27.42% and 25.99% more BW, respectively, compared with JNC. The CBK and BCBK animals consumed significantly less DM than JNC to gain a unit of BW. Body weight gain, DM intake and feed conversion efficiency were similar between CBK and BCBK. Weight of hot and cold carcass, ribs, boneless meat and Longissimus dorsi muscle area were significantly different among JNC and its crossbreds. The heaviest carcass was observed in CBK followed by BCBK and JNC. Carcass, chest and femur lengths were greater in CBK and BCBK compared with JNC. Chest width, chest depth and hip width were similar in JNC and its crossbreds. Femur width was significantly greater in CBK compared with BCBK and JNC. Femur depth and chest girth were significantly greater in CBK and BCBK compared with JNC. Weight and fat yield in different carcass cuts were greater in crossbreds compared to JNC. Percent moisture, crude ash, and crude protein of meat were similar in JNC and its crossbreds. Percent crude fat in beef was significantly greater in JNC compared with its crossbreds. Beef shear force value, percent water holding capacity, juiciness and tenderness were significantly greater for JNC compared to its crossbreds. In conclusion, CBK and BCBK have shown greater growth rates and produced heavier carcasses with good degree of fatness when compared with JNC.