• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.917-922
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• 2003

This study was conducted to investigate effects of farm, type of sow Landrace${\times}$Yorkshire, LY; Yorkshire${\times}$ Landrace, YL; Yorkshire${\times}$Yorkshire, YY and multi-cross bred sow, MBS), parity, farrowing year, farrowing season and mating method on reproductive traits such as total number born per litter, number of born alive per litter, number of weaned per litter, number of mummified per litter, number of stillbirth. The Reproductive Records of 3,387 litters from January, 1999 to September, 2002 were obtained from four pig farms managed by Electronic Data Processing(EDP) system. Reproductive performances for two types of F1 Sows(YL and LY) were estimated as 11.34${\pm}$0.266 and 11.57${\pm}$0.263 heads for total number of born per litter, 10.56${\pm}$0.216 and 11.81${\pm}$0.251 heads for number of born alive per litter, and 10.05${\pm}$0.131 and 9.96${\pm}$0.153 heads for number of weaned per litter, respectively. These records are significantly higher(P〈0.05) than those of YY and MBS. However, number of mummified per litter, number of stillborn per litter, number of dead by diarrhea per litter, number of dead by other reasons during lactating period per litter and estrus interval did not show significant difference between types of sows. There were more total number of born per litter and number of weaned per litter in year 2001 than other year. As year passed, number of dead by crush per litter, number of dead by diarrhea per litter and number of dead by other reasons during lactating period per litter reduced from 0.18${\pm}$0.023 to 0.07${\pm}$0.022 head, 0.12${\pm}$0.21 to 0.02${\pm}$0.020 head and 0.43${\pm}$0.041 to 0.22${\pm}$0.040 head, respectively and weaning rate increased from 0.94${\pm}$0.005% to 0.97${\pm}$0.005%. The total number of born per litter and number of born alive per litter were higher(P〈0.05) in the case of mating twice than mating once. The number of weaned per litter, number of mummified per litter and number of stillborn per litter were not significant(P〈0.05) between mating methods. Estrus interval was shorter on the occasion of twice artificial insemination(5.24${\pm}$0.153days) than twice natural mating(6.51${\pm}$0.466days).

• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.925-936
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• 2005

The present study evaluated whether pentoxifylline added to the freezing extender could improve the sperm characteristics and function in canine frozen semen. Also the conception rate following AI with frozen-thawed semen was investigated. The beneficial effects of pentoxifylline supplementation were visible in motility, viability, acrosome reaction, and tail swelling patterns. Especially, highest sperm viability and function were obtained in the forzen semen supplemented with 1mM pentoxifylline. The follicle size measured by ultrasonography was 6.48 mm, 11.52 mm and 8.9 mm on 11, 13 and 15 days after the onset of natural estrus, respectively and ovulation occurred on 13 and 15 days. The pregnancy rates in bitches inseminated with frozen semen on natural and induced estrus were 71.4% and 75.0%, respectively. There was no significant difference between the pregnancy rates in bitches inseminated with frozen semen following natural and induced estrus, but the litter size was slightly increased in natural cycle.

• Korean Journal of Ichthyology
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• v.3 no.1
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• pp.1-10
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• 1991

Spawning behavior and development of eggs and larvae of Mugilogobius abei were observed in the laboratory at Pusan, Korea. The adult male of Mugilogobius abei was observed making nest-like spawning-bed to lay eggs and showing territorial and courtship behaviors. The eggs were transparent and spherical in shape, measuring 0.40~0.50 mm in diameter. They have a bundle of adhesive filaments at their basal end and a cluster of small oil globules. The eggs became ellipsoid shape after the insemination and measured about 0.93~0.96 mm on the long axis. Hatching began about 110 hours after fertilization at water temperature of $24.5{\sim}25.5^{\circ}C$. The newly hatched larvae were 2.04~2.10 mm in total length, with 24~25(8~9+16) myomeres. Many melanophore and guanophore are distributed on eye cups, gas bladder, optic vesicle and the caudal region. Four days after hatching the yolk and oil-globule were completely absorbed and the larvae attained a total length 2.20~2.35 mm. The larvae swam actively in the aquarium and start to practice feeding on the rotifer. Twelve days after hatching, the larvae averaged 3.20 mm in TL and the caudal notochord flex at $45^{\circ}$. Rudimental second dorsal, anal, caudal and ventral fins are also formed. The larvae attained 10.40~10.80 mm in TL, 35 days after hatching, are found to start the bottom-life after having completely formed first dorsal and ventral fins. The larvae reached the juvenile stage at 50~60 days after hatching and attained 15.37~20.25 mm in TL. At this period all scales appeared on the body.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.4
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• pp.387-392
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• 2016

This study was conducted to ameliorate the productive performance of Hanwoo cow using hill pasture grazing system. Twenty seven animals aged 9 were designed to divide with 3 groups which were Control (restricted concentrate and hay for 5 months), T1 (restricted concentrate and pasture grazing ad libitum for 5 months) and T2 (restricted concentrate, 30 g/head of mineral nutrients and pasture grazing ad libitum for 5 months). Average daily gains increased higher in the order of T2, Control and T1. The conception rate of Control, T1 and T2 after artificial insemination during post-grazing period were 88.9%, 100%, 100%, respectively. In conclusion, the results indicated that hill pasture grazing system might enhance not only the productive performance of Hanwoo cow but also profit for conventional feeding system.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.253-261
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• 2002

This study was carried out to investigate the semen characteristic, motility and viability and sperm motion characteristic by CASA test for establishing the Jindo-dog's semen freezing system. The results obtained are as follow: 1. The semen was collected 63 times. Average volume of semen, concentration of sperm, total number of sperm, progressive motility and viability were 3.8 $m\ell$ 145.6$\times$10$^{6}$ cells/$m\ell$, 396.2 x10$^{8}$ cells, 79.7% and 89.5%, respectively. Also, Fawn (Yellow) Jindo-dog comparing with White Jindo-dog showed better concentration of sperm, total number of sperm, progressive motility and viability. Among all dogs, the results of No. 2 Fawn Jindo-dog were the best. 2. The average progressive motility and viability of semen from 46 times were 73.5%, 82.3% before freezing and 51.1%, 64.9% after freezing. So, the freezing of semen has affected the progressive motility and viability. The progressive motility and viability of Fawn Jindo-dog's semen, before and after freezing, were better than White Jindo-dog. And No. 2 Fawn Jindo-dog showed the best results and showed significantly different among all dogs (P<0.05). 3. The 44 times-tested .esults by CASA system were as follow; MOT (motility) 65.6%. PROG (progressive motility) 54.8%, VAP (average path velocity) 75.3 ${\mu}{\textrm}{m}$/sec, VCL (curve linear velocity) 90.0 ${\mu}{\textrm}{m}$/sec, VSL (straight-line velocity) 69.4 ${\mu}{\textrm}{m}$/sec and ALH (amplitude of lateral head displacement) 4.4 ${\mu}{\textrm}{m}$. Although the motion characteristic of frozen semen were not significantly different between White and Fawn Jindo-dog, No. 2 Fawn Jindo-dog showed the best results and was significantly different among all dogs (P<0.05). 4. The success rate of frozen semen production between White and Fawn Jindo-dog were 43% (13/28), 94% (33/35), respectively, and the total success rate was 73% (46/63). The freezing-ability of Fawn Jindo-dog's semen was better than the other. Conclusively, the present results indicated that the characteristic and motility of Jindo-dog') semen were suitable for processing frozen semen, artificial insemination and mass production system. Also, the selection of suitable dog-breed was so important because the characteristic and freezing-ability of semen were significantly different between White and Fawn Jindo-dogs and among all individual dogs.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.291-298
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• 2002

This study was carried out to investigate the reproductive status and the effect of progesterone treatment on the recovery of reproductive disorders in Hanwoo. Hanwoo farms were surveyed the general management status, such as the type of barn, the feed intake, the incidence of reproductive disorders. The reproductive disorder cattle were treated 7 days insertion of control internal drug releasing for cattle (CIDR) and injection of PGF$_2$$\alpha$ at 1 day before removal. The recovery of reproductive disorders was assessed by determining the pregnancy following artificial insemination. The total number of surveyed Hanwoo farms was 127, and the total incidence rate of reproductive disorders was 19.7% (209/1,061). Compared to the herd size, the incidence rates of reproductive disorders in less than 10 heads (37.5%) was significantly (P<0.05) higher than in 10 to 20 heads (14.7%) and more than 20 heads (13.6%) of herd size per farm. The incidence rate of reproductive disorders in tie stall barn was significantly (P<0.05) higher than in free stall barn (30.4% vs. 14.7%), and even in free stall barn, that tended to decrease as the floor area was larger. The incidence rate of reproductive disorder by the parity was highest in heifer (50.7%), and that tended to decrease as the parity was increased. The distribution rate of the case of reproductive disorder in anestrus, recovery rate of reproductive disorders fellowing CIDR treatment was 75.1% (157/209). In the recovery rate of reproductive disorders by body condition score (BCS), BCS 1, 2 and 3 was significantly (P<0.05) higher pregnancy rate (85.7, 84.9 and 86.8%), and gross recovery rate in emaciated cattle was better than in obese cattle (BCS 4, 5). In conclusion, the incidence rate of reproductive disorders in Hanwoo raised in Youngju province area was 19.7%, and that tended to decrease as the herd size was increase because of increased farmer's attentions, and the floor area was larger. The majority of the case of reproductive disorders was anestrus. The recovery rate of reproductive disorders following CIDR treatment was 75.1%, and to optimize the recovery rate of reproductive disorders, cows and heifers were maintained BCS 1, 2 and 3 by moderate management.

• Journal of Embryo Transfer
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• v.20 no.1
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• pp.25-33
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• 2005

This study is a part of research that development of effective genetic resources preservation system using the in vitro spermatogenesis, in vitro insemination and culture system. We aimed for establishment of in vitro culture system with in vitro activated porcine oocytes. The porcine oocytes were matured for 48 hours in $TCM199+10\%$ FCS and activated with $7\%$ ethanol. The activated oocytes were cultured for 7 days in $TCM199+10\%$ FCS or $NCSU23+0.4\%$ BSA medium. The activated oocytes were not developed to the blastocyst stage in $TCM199+10\%$ FCS medium. However in $NCSU23+0.4\%$ medium, those were developed to blastocyst with $3\%$ of treated oocytes. We extended maturation duration of porcine follicular oocytes fur 48, 52, 56, 60, 64, 68, and 72 hours and activated with $7\%$ ethanol and cultured using $NCSU23+0.4\%$ BSA medium. The six percents of activated oocytes were developed to blastocyst in 48 hours and $10\%$ in 52 hours with comparatively low rates suggested to be not fully activated by regenerated MPF. Maturation durations from 56 hours to 68 hours supported to develop upto $11.9\~18.3\%$ of blastocysts. However the developmental rate was declined to $7.2\%$ at 72 hours of maturation duration because of cytoplasmic deterioration. The assumed time window for activation will be $56\~68$ hours of maturation duration. When the matured oocytes were activated with electric pulse of 1, 1.2, 1.4, 1.6, 1.8 and 2.0kV/cm for $80{\mu}s$, although appling the electric current once was not enough for activation, appling twice with 1.6kV/cm for $80{\mu}s$ was shown the highest developmental rate with $11.3\%$. When those were compared with activating methods, $15.7%$ of blastocyst rate was obtained in the $7\%$ ethanol. That was higher than those in electric pulse with $9.5\%$ and calcium ionophore method with $5.8\%$. In this experimental condition, the $7\%$ ethanol treatment was the most effective method for activating porcine oocytes.

• Development and Reproduction
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• v.11 no.2
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• pp.127-135
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• 2007

In order to monitor the developmental features of embryos, larvae, and juveniles of Konoshiro Gizzard Shad Konosirus punctatus, the fertilized eggs were gotton using artificial insemination. Konosirus punctatus were caught in Mankyung-myeon, Kimjae, Jeollabuk-do at June of 2004, and experiments were carried out in Ichthyology laboratory in Chonnam National University. Konosirus punctatus spawned draft egg from March to June. The fertilized eggs were cultured in $19.0{\sim}23.0^{\circ}C$(mean, $21.2^{\circ}C)$. The eggs had spherical shape and the diameter is $1.14{\sim}1.34\;mm$(mean, 1.21 mm). The lens began to appear from 35 hr 53 min after fertilization. At the moment, the movement of larvae was more active, and the tail was separated completely from yolk, the heart had forms, and melanophore appeared. Hatching was observed from 37 hr 10 min after fertilization. The total length of the hatched larvae was $4.26{\sim}5.30\;mm$(mean, 4.96 mm), but the mouth and anus were not opened at the time when the larva had yolk sack, and had $22{\sim}27$ myometium, and the anus located just abdominal front of the tail fin, and melanophore accumulated in the eye. Post-larvae used yolk completely after 2 day of hatching, and the total length was $4.96{\sim}5.74\;mm$(mean, 5.24 mm). From 16 days after hatching, the tail had curved tail end, and appeared the stems for pectoral, dorsal, and caudal fins. At 53 days after hatching, the total length of post-larvae was $27.11{\sim}34.09\;mm$(mean, 30.11 mm), and the frontal part of head developed like an adult one. At this time, fins and body are transferred to those of adult Konosirus punctatus. Fishes have a different shape and location of melanophore even in the same family. This research was tried to elucidate the early developmental features of Konosirus punctatus, together with species-specific pattern of melanophore.

• Journal of Embryo Transfer
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• v.25 no.3
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• pp.145-148
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• 2010

Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade I and II (G I and G II) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G I blastocysts were significantly higher than those of G II blastocysts ($10.2{\times}10^{15}/mol\;s^{-1}$ versus $6.4{\times}10^{15}/mol\;s^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\sim}10^{15}/mol\;s^{-1}$ respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\times}10^{15}/mol\;s^{-1}$, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.

• Journal of Embryo Transfer
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• v.22 no.3
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• pp.195-198
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• 2007

This study was carried out to investigate the effects of hormones treated (progesterone, and combination of progesterone and estradiol) on the reproductive performance of dairy cattle. The intravaginal CIDR was inserted in the vagina of cow on day 14 (P-7, PE-7), 16 (P-5, PE-5) and 18 (P-3, PE-3) post-insemination to stimulate progesterone secretion with (PE) and without estradiol(P). The CIDR was removed on 7 (P-7, PE-7), 5 (P-5, PE-5) and 3 (P-3, PE-3) days after. The cows for control group were not treated with CIDR or hormones. Conception rate, the duration needed for conception after AI and the number of AI services needed for conception were measured. Conception rate in control, CIDR without estradiol (P-7, P-5 and P-3) and CIDR treated (PE-7, PE-5 and PE-3) cows were 15.8%, 40.0%, 31.5%, 28.6%, 62.5%, 50.0% and 0%, respectively. The days needed for conception after AI in control, P-7, P-5, p-3, PE-7, PE-5 and PE-3 were 64.9, 63.0, 59.1, 8.0, 0.0, 18.9 and 83.3 days, respectively. The days needed for conception in cows treated with CIDR (PE-7) has shown longer than control and PE-3 (p<0.05). The number of AI services needed for conception in control, CIDR (PE-7, PE-5 and PE-3) and CIDR without estradiol treated cows (P-7, P-5 and P-3) were 27, 2.3, 1.9, 1.3, 1.0, 1.7 and 2.8 times, respectively. The number of AI services needed for conception have shown significantly lower in PE-7 compared to control and PE-3.