• Journal of Life Science
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• v.29 no.9
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• pp.996-1001
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• 2019

Edible insects are reported to be rich in protein, minerals and vitamins, and much attention has been paid to them as a future food source. In Korea, they were massively reared and sold. In order to enhance the market value of edible insects for industrialized mass production, it is important to develop the safe and nutritious feed sources for rearing them are needed. In this study, a chlorella-free control feed (Exp1) and six experimental feeds supplemented with 0.5~2.0% liquid or powder types of chlorella were formulated. Protaetia brevitarsis and Allomyrina dichotoma, registered as food ingredients in Korea, were fed with the designed feeds and parameters of growth including larval survivorship, larval body weight, and larval period were analyzed. When chlorella added, larval survivorship was increased 2~13%(p>0.05) and 9~22%(p<0.05) in each beetle compared to the control. Interestingly, the larval period of chlorella powder-added groups was shortened by 24 days (Exp3, p<0.05) in P. brevitarsis and 19 days (Exp4, p<0.01) in A. dichotoma. Meanwhile, some parameters, crude protein, crude fiber, copper, zinc, potassium, magnesium, and phosphorous, in chlorella-added groups of P. brevitarsis were also higher than the control group. Therefore, chlorella could promote the larval growth performance of these two beetles and be used as a feed additive in rearing them.

• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• Journal of Institute of Control, Robotics and Systems
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• v.20 no.10
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• pp.1079-1083
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• 2014

In this paper, a new zone radius determination algorithm is proposed for a nature-inspired routing protocol that emulates the foraging behavior of bees based on their ability to find an optimal route from nectar sites. Instead of changing the radius of nodes one-hop by one-hop, the proposed algorithm alters the radius of nodes as gaps of another radius and adapt quickly to network conditions. The simulation results show that the proposed algorithm has higher efficiency compared with existing studies in an aspect of computational complexity and end-to-end delay.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.77-85
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• 2017

Insect pests have been one of the main constraints affecting turf sod cultivation areas, playgrounds, parks, golf courses, airports and other related recreational grounds. However, turfgrass research has been very limited and confined within a limited period of time compared to other branches of crop science in Korea. This review was surveyed from all papers in KCI (Korea Citation Index), SCI (Scientific Citation Index) and SCIE (SCI Expended) journals related to turfgrass research in Korea. Fifty two papers concerning turfgrass insect pest in Korea have hitherto been published in 8 different scientific journals since 1990. Thirty three papers (63.5% of total) were published in Weed and Turfgrass Science. The main topics of focus were divided into two parts; ecological study and control of insect pests. Before the 1990 decade, there were no scientific papers published in relation to turfgrass insect pest science. However, during the 1990, 2000 and 2010 decades, 9, 10 and 14 papers were published respectively in Weed and Turfgrass Science. From 1997, about 2 papers were published per year in the scientific journals. Thirty three insect pest species belonging to 13 families in 6 orders, one Eriophyidae mite, Aceria zoysia and one Armadillidae sowbug, Armadillidium vulgare, have hitherto been listed as turfgrass arthropod pests in Korea. Fifty percent of turfgrass insect pest papers published were focused on whitegrub, Adoretus tenuimaculatus, Popillia quadrigutta and earthworm research in Korea.

• Journal of Life Science
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• v.27 no.10
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• pp.1185-1190
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• 2017

Insects are gaining recognition as an alternative source of protein. As a result, more and more domestic farms have begun mass rearing of edible insects. In order to produce high quality insects, studies on the development of safe and nutritious feed sources are needed. Given the cost of rearing insects, agricultural and industrial by-products are good sources for feed. The efficient utilization of these by-products can help in reducing the cost of production and preventing environmental pollution. In the current study, Citrus unshiu peel (CP), soybean curd cake (SCC), soybean oil meal (SOM), and brewers dried grain (BDG) were investigated for their effects on larval growth and development of Protaetia brevitarsis. Interestingly, the feed with 10% SCC increased larval weight by 3.5 times. For the larval period, the group of 10% SCC was significantly shorter than the control. Furthermore, minerals such as Zn, Ca, K, Mg, Na, and P were recorded to be high in 10% SCC. A total of 17 amino acids were present in 10% SCC, of which tyrosine and arginine were predominant. The heavy metal contents were very small amounts or not detected in any of the investigated groups. These findings provided a scientific basis for the utilization of soybean curd cake as a nutritional feed source to promote larval growth and produce quality insects.

• Food Science of Animal Resources
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• v.40 no.5
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• pp.795-812
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• 2020

Novel food sources have enormous potential as nutritional supplements. For instance, edible insects are considered as an alternative food source due to their higher protein content; moreover, they are economically efficient reproducers and have high in nutritional value. In this study, we investigated the toxicity of the freeze-dried powder of Locusta migratoria (fdLM), known to contain rich proteins as well as fatty acids. The objective of the present study was to evaluate the subacute toxicity of fdLM in male and female Sprague-Dawley (SD) rats. The SD rats were divided into four groups based on the dosage of fdLM administered: dosage of 0 (vehicle control), 750, 1,500, and 3,000 mg/kg/day were administered for 28 days. Toxicological assessments including observations on food consumption, body and organ weights, clinical signs, mortality, ophthalmologic tests, urinalyses, hematologic tests, clinical chemistry tests, gross findings, and histopathology tests were performed. Clinical signs, urinalyses, hematology, serum biochemistry tests, and organ weight examinations revealed no fdLM-related toxicity. The no-observed-adverse-effect level for fdLM was higher than 3,000 mg/kg/day in rats of both sexes; therefore, fdLM, in conclusion, can be considered safe as an edible alternative human and animal food source material.

• Korean journal of applied entomology
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• v.13 no.4 s.21
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• pp.209-216
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• 1974

Life history and control methods of Aphanostigma iakusuiense Kishida were studied during April to November, 1974, in the Naju pear orchard area, Cholla Namdo. The results are summarized as follows: 1. More than $60\%$ of wintering eggs were found at the height of 1.5 to 2.0m from the soil surface, with approximately $10\%$ at 0.5 to 1.5m and 1.5 to 2.5m respectively. 2. There was little difference in the size of egg masses and hatching ratio $(12.7-13.7\%)$ between the varieties of host trees, Mansamkil and Kooksoo. 3. Hatching was not observed until April 6, but hatches of $12.8\%$ on April 13 and $90.6\%$ on May 4 were found. 4. During the hatching season in early spring, 8 chemicals were applied to test effectiveness of control. C8514, Metasystox, Acar 338, and Parathion were more effective than either Sevin or Malix for control of this insect. 5. Highest populations of pear phylloxera ocurred from August 20 to September 20 at which time levels of eggs and larvae were simillar in abundance. 6. In tests for control of this insect after in paper bags, DDVP, Loxon, C8514, Sevin, and Binapacryl were more effective than either EPN or Otran. 7. Predacious mite, Protolaelape pygmaeus Miller, and several species of lady beetles (Coccinellidae) preying on this insect as natural enemies were collected. The predation ratio of mites varied according to region: that is 0.77 to $18.6\%$ in Bia Orchard; $7.88-48.7\%$ in Noan Orchard and almost none in Youngsanoo Orchard. 8. The varietal difference in fruit damage by this insect was in the order of Choseng Chuck, Kooksoo, followed by Sin Ko, Mansamkil, Keumchon Choo, and Changsimrang.

• BMB Reports
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• v.41 no.5
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• pp.388-393
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• 2008

The Pphsc70 (heat shock cognate 70) gene was isolated from the endoparasitoid Pteromalus puparum and then characterized. The full-length cDNA was 2204 base pair (bp) and contained a single 1968 bp ORF that encoded a polypeptide of 656 amino acids with a predicted molecular mass of 71.28 kDa. Phylogenetic analysis based on Hsc70 amino acid sequences from fifteen insect species agreed with the present phylogeny. In addition, genomic DNA confirmed the presence of three introns located at the coding region as well as the 5'UTR. A significant elevation of Pphsc70 expression was observed following heat treatment, however, continued exposure to heat shock or recovery caused the expression of induced mRNA to gradually decline to levels that were significantly lower than those of control pupae (P < 0.05). In addition, a significant increase was observed in the emergence rate of pupae that were preheated at $40^{\circ}C$ and then exposed to $50^{\circ}C$ for 1 h when compared with the pupae that were not preheated, but instead directly exposed to $50^{\circ}C$. Taken together, these results revealed that exposure to gradually increasing temperatures can enhance an insects thermo-tolerance.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.407-414
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• 1997

The resistance of a non-transgenic poplar clone, 'Ogy' and three transgenic poplar lines to the cottonwood leaf beetle, Chrysomela scripta F., was evaluated by in vitro feeding. The lines were transformed with neomycin phosphotransferase II(NPT II) as a selectable marker, proteinase inhibitor II(pin2) as a resistance gene, and CaMV 35S as a promoter. An efficient method of sterilizing the beetle eggs and introducing them into plant tissue cultures was developed. The resistance of the transgenic lines was investigated in terms of effects tin leaf area consumed, insect weight, insect developmental stages, and plantlet root dry weight after feeding. Also, leaf area consumed was examined by leaf age as measured through leaf plastochron index(LPI). The leaf area consumed and insect weight were highly significant between transformants and control, and insect development in vitro was significant among the transgenic lines. Larval infestation was the most severe around LPI 4 to 5 which were young leaves. The system provided a quick, highly controlled method to screen developing transgenic plantlets directly.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.624-633
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• 2021

This study was conducted to determine the supplemental effects of two insect meals, mealworm (MW) and black soldier fly (BSF), with high or low lipid levels in diets, on Pacific white shrimp Litopenaeus vannamei. Sardine and tuna by-product meals were used as the fish meal source in a control (Con) diet. The fish meals were replaced with MW, defatted MW (deMW), BSF or defatted BSF (deBSF), respectively. The shrimp (body weight: 0.47 g) were stocked into 20 acryl tanks (215 L) and fed the diets six times a day. After 45 days of the feeding trial, the shrimp that were fed insect meals had significantly higher phenoloxidase and superoxide dismutase activities than the shrimp fed Con diet. The gene expressions of prophenoloxidase, crustin and penaeidine-3c in shrimp hepatopancrease were also higher in shrimp that were fed the insect diets, regardless of defatting than those in shirmp that were fed Con diet. The survival against Vibrio parahaemolyticus was higher in shrimp that were fed the diets containing defatted insect meals than in shrimp that were fed Con diet. These results indicate that MW and BSF, regardless of lipid levels, could be good protein sources for the enhancement of innate immunity and anti-oxidant capacity of the shrimp.