• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.89-93
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• 2004

Expression of a fusion protein between B. thuringiensis crystal protein, Cry1Ac1 and a scorpion insect toxin (AaIT, Androctonus australis Hector insect toxin) in acrystalliferous B. thuringiensis strain (Cry-B strain) was examined. The cry 1Ac1 gene was cloned in B. thuringiensis-E coli shuttle vector, pHT3101, under the control of the native cry 1Ac1 gene promoter (pProAc) and a gene encoding AaIT was inserted in XhoI site in the middle of the cry 1Ac1 gene (pProAc-ScoR). B. thuringiensis Cry-B strain carrying pProAc-ScoR (PyoAc-ScoR/CB) produced an inclusion body of irregular shape and the expressed fusion protein is approximately 65 kDa in size. Sporulated cells and spore-crystal mixtures of ProAc-ScoR/CB had insecticidal activity against Plutella xylostella larvae, showing $LT_50$ of ProAc-ScoR/CB (22.59 hrs) lower than that of ProAc/CB (30.06 hrs) at $1{\times}{10^7} {CEU/cm^2}$. These results suggest that the fusion protein including a B. thuringiensis crystal protein and an AaIT may be functionally expressed in B. thupingiensis. Moreover, we verified the additive toxicity of AaIT, which is a new feasible candidate for insect control.

• Mycobiology
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• 제48권2호
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• pp.139-147
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• 2020

Root-feeding Scarabaeidae, particularly white grubs are considered among the most harmful coleopteran insect pests in turfgrass. In this work, sixteen entomopathogenic fungal species were assayed against flower chafer beetle, Protaetia brevitarsis (Coleoptera: Scarabaeidae) and Metarhizium anisopliae JEF-314 showed high virulence. The control ability of the isolate JEF-314 has been in detail tested for a model insect flower chafer beetle. Further analyses showed insect stage-dependent virulence where the fungal virulence was the highest against smaller instar larvae. Additionally, we confirmed that millet-based solid cultured granule was effective against the soil-dwelling larval stage. The isolate also showed a similar ability for a representative pest (Popillia spp.) in laboratory conditions. Our results clearly suggest a high potential of M. anisopliae JEF-314 to control the flower chafer beetle, possibly resulting in controlling of root-feeding white grubs in turfgrass. Based on the insect life cycle and susceptibility to the fungus, late spring and summer time would be the optimum time to apply JEF-314 granules for an effective control. Further characterization of the efficacy of the fungus under field conditions against the Scarabaeidae beetles might provide an efficient tool to control this beetle in an environment-friendly way.

• 한국응용곤충학회지
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• 제22권2호
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• pp.98-105
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• 1983

The rapid increase in cases of insect resistance to insecticides indicates that the contribution of present chemical control practices inevitably leads to exhaustion of available insecticide resources against key insect species. Now the problem of insecticide resistance exists worldwide among insects and mites affecting field crops and animals including human beings, ranging from minimal or absent in some developing countries, where use of insecticides has been low, to extremely severe in many developed countries. Since the occurrence of insect resistance to insecticides was firstly recognized in 1908, the increase in recent decades has been almost linear and now the number of species of insects and acarines in which resistant strains have evolved have been increased to a total of 432. Of these, $261(60\%)$ are agricultural importance and $171(40\%)$ of medical/veterinary importance. The phenomenon of insecticide resistance is asserting itself as the greatest challenge to effective chemical control of many important insect pests. Resistance of insects to insecticides has a history of nearly 80 years, but its greatest increase and its strongest impact have occurred during the last 40 years following the discovery and extensive use of synthetic organic insecticides and acaricides. The impact of resistance should be considered not only in terms of greater cost of pest control due to increased dosages and number of applications but also in terms of the ecological disruption of pest-beneficial species density relationships, the loss of investment in the development of the insecticides concerned, and socio-economic disruption in agricultural communities. Despite its grave economic consequences, the phenomenon of insecticide resistance has received surprisingly little attention in Korea. Since the study of insecticides started firstly in 1963, many entomologists have been concerned with this study. According to their results, some of the rice pests and some of the mites on orchard trees, for example, have developed worrisome level of resistance in several areas of this peninsula. With many arthropods, considerable advances in the developed countries have been made in the study of the biochemical and physiological mechanisms of resistance. Progress involves the biochemical characteristics of specific defense mechanisms, their genetics, interactions, and their quantitative and qualitative contribution to resistance. But their studies arc still inadequately known and relatively little have been contributed in terms of unique schemes of population management in achieving satisfactory pest control. It is apparent that there is no easy solution to resistance as a general phenomenon. For future challenging to effective control of insect pests which are resistant to the insecticides concerned, new insecticide groups with distinctly novel mode of action are urgently needed. It is clear, however, that a great understanding of the factors which govern the intensity of selection of field population for resistance could lead to far more permanently successive use of chemicals within the framework of integrated pest management than heretofore practiced.

• 한국유기농업학회:학술대회논문집
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• 한국유기농학회 2009년도 하반기 학술대회
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• pp.45-56
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• 2009

2005년부터 2008년에 걸쳐 무농약 유기 농산물 인증을 받은 사과원의 변동상황을 조사하였고, 무농약 유기재배 사과원에서 병해충 발생과 관리 실태 파악을 목적으로, 2006년에는 17명을, 2009년에는 13명을 대상으로 심각한 피해를 야기하는 문제병해충과 병해충 관리를 위해서 사용하는 친환경 농자재에 대해서 설문조사를 실시하였다. 또한, 2007년에는 무농약 유기 농산물 인증을 받은 음성 무주 장성 영주 의성 청송 칠곡 지역의 10개 사과원을 대상으로, 2009년에는 충주 예산 영덕 지역의 3개 사과원을 추가한 13개 사과원을 대상으로 피해를 많이 주고 있는 병해충의 발생상황과 병해충 관리를 위해서 사용하는 친환경 농자재에 대해서 방문조사를 실시하여 다음과 같은 결과를 얻었다. 2005~2008년에 걸쳐 사과에 무농약농산물 인증을 받은 농가는 각각 9, 24, 42, 48농가였으며, 유기농산물(전환기유기농산물 포함) 인증을 받은 농가는 각각 5, 4, 11, 30농가로서, 2005년 대비 2008년에 무농약농산물은 5.3배, 유기농산물은 6배가 증가하였다. 무농약 유기 농산물 인증 사과원에서 문제병해는 갈색무늬병, 탄저병, 겹무늬썩음병, 그을음(점무늬)병이었는데, 석회유황합제와 석회보르도액을 사용하는 사과원의 경우에 2006년과 2007년에 비하여 2009년에는 갈색무늬병, 탄저병, 겹무늬썩음병의 피해가 적었고, 그을음(점무늬)병은 심하지는 않았지만 문제가 되었다. 문제해충은 2006년과 2007년에는 사과혹진딧물, 복숭아순나방, 복숭아심식나방이었으나, 2009년은 교미교란제, 난황유, 기계유유제를 사용하는 사과원의 경우에 이들 해충의 피해는 크게 적어진 반면에, 일부 사과원에서 사과유리나방과 사과면충이 새롭게 문제 되었다.

• 한국응용곤충학회지
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• 제45권3호
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• pp.241-259
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• 2006

폴리드나바이러스는 고치벌 및 맵시벌류에 공생하는 DNA 바이러스로 기주 염색체에 프로바이러스 형태로 존재한다. 이 바이러스의 복제는 기주 용발육시기에 난소받침 상피세포에서 시작되어 유리 바이러스 형태의 입자 구조를 이루게 된다. 바이러스 입자는 기주가 피기생체에 산란할 때 알과 함께 혈강으로 옮겨진다. 이 바이러스 게놈의 염기서열을 바탕으로 여러 폴리드나바이러스 유전자군이 동정되었으며, 이들의 생리적 기능도 알려지고 있다. 본 종설은 기생 생리적 견지에서 폴리드나바이러스 게놈을 특성화하고, 이를 토대로 생리 교란 유전자들을 응용할 수 있는 새로운 해충 방제 전략을 소개한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.59-62
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• 2016

Many insects have gained increasing attention as an alternative protein for humans. Among those, the two-spotted cricket, Gryllus bimaculatus (Orthoptera: Gryllidae), was recently approved as a general food ingredient by the Korean Ministry of Food and Drug Safety. For industrial utilization of G. bimaculatus, mass rearing techniques and production system should be standardized first. In this study, we investigated the effects of five different feeds on the growth and development of G. bimaculatus. Feed is the one of the key factors that has considerable effects on rearing insects. With five different kinds of feed on $1^{st}$, $3^{rd}$, and $5^{th}$ instar nymphs, the change of survival rate, body weight were monitored up to eight wk after hatching. We concluded that 50% of soybean flour, 20% of corn powder, 10% of rice bran, 9% of milk serum, 10% of rice flour, 0.5% of microorganisms, and 0.5% of multivitamins and minerals (diet C) was the best mix for promoting growth and development of $3^{rd}$ instar nymphs compared to the control diet.

• 한국축산식품학회지
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• 제41권2호
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• pp.185-195
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• 2021

The objective of this study was to determine the effects of high pressure to investigate the technical functional properties of the protein solution extracted from an edible insect, Protaetia brevitarsis seulensis. High pressure processing was performed at 0 (control), 100, 200, 300, 400, and 500 MPa at 35℃. The essential amino acid index of the control was lower (p<0.05) than that of the P. brevitarsis seulensis extract treated with 100 MPa. The SDS-PAGE patterns tended to become faint at approximately 75 kDa and thicker at approximately 37 KDa after high pressure treatment. The protein solubility and pH of the protein tended to increase as the hydrostatic pressure levels increased. The instrument color values (redness and yellowness) of the P. brevitarsis seulensis protein treated with high pressure were lower (p<0.05) than those of the control. The forming capacity of the protein solution with P. brevitarsis seulensis treated with high pressure was higher (p<0.05) than that of the control. In conclusion, we confirmed that the technical functional properties of edible insect proteins extracted under high pressure of 200 MPa are improved. Our results indicate that high pressure can improve the technical functional properties of proteins from edible insects.

• 식물병연구
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• 제21권4호
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• pp.273-279
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• 2015

딸기 육묘기에 발생하는 주요 병해충은 탄저병, 흰가루병, 시들음병, 점박이응애, 진딧물 등이 있다. 친환경과 약제 처리 방제력은 병해충이 발생할 때 처리하는 방제프로그램과 비교하여 적은 유기농업자재와 살균 살충제 사용량으로 병해충방제 효과를 높일 수 있다. 2012년과 2013년 딸기 육묘기에 설향 품종을 대상으로 병해충 방제를 위한 친환경 방제력(EFSC)과 약제 방제력(CSC)효과 시험을 실시하였다. EFSC는 육묘기간 동안 무처리 대비 병해충 발생이 감소되었다. 탄저병과 흰가루병 발생은 EFSC와 CSC처리에서 2012년과 2013년 시험동안 비슷한 이병율을 보였다. 점박이응애 발생은 2013년에 EFSC와 CSC처리간 비슷하였고 6월 중순에 높은 피해율을 보였다. 진딧물 발생은 EFSC에서 6월 초중순에 높은 발생율을 보였다. 이러한 결과로 EFSC처리는 친환경으로 딸기에 발생하는 병해충을 효과적으로 방제하는 데 CSC를 대체할 수 있을 정도로 도움을 줄 수 있을 것이다.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 2004년도 춘계학술대회 논문집
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• pp.263-272
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• 2004

• Nutrition Research and Practice
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• 제15권1호
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• pp.122-135
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• 2021

BACKGROUND/OBJECTIVES: The purpose of this study was to measure consumer acceptance of edible insect foods (EIFs) while applying the extended theory of planned behavior (ETPB). Insects as food have attracted interest as potential possible sources of nutrition for the future. This study investigated consumers' perception toward insect food and future purchase behaviors. SUBJECTS/METHODS: A survey was conducted among citizens of Korea. About two thirds of respondents had tried an EIF previously, and the mean value for food neophobia was 3.1 on a 5-point Likert scale. Respondents were divided into 2 groups of those experienced with EIFs and those not and by level of food neophobia. An independent t-test, multiple regression and descriptive analyses were conducted on the data. RESULTS: The primary reason for not eating EIFs was that their insect form induced disgust. Comparisons of ETPB attributes by experience with EIFs showed significant differences between groups for food neophobia, subjective norm, attitude, and behavioral intention. In addition, significant differences were found for subjective norm, perceived behavioral control, attitude, and behavioral intention between 2 neophobia groups. Finally, the results of measuring the relationships between ETPB attributes and behavioral intention showed only subjective norm and attitude affected behavioral intention. CONCLUSIONS: These results suggest that enhanced subjective norms producing a publicly accepted EIFs environment would help encourage the purchase of EIFs. The results of this study can help the development of EIF products for future food markets.