International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Expression of a Fusion Protein with Cry1Ac Protein and a Scorpion Insect Toxin in Acrystalliferous Bacillus thuringiensis Strain

  • Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National University) ;
  • Li, Ming-Shun (School of Agricultural Biotechnology, Seoul National University) ;
  • Chang, Jin-Hee (School of Agricultural Biotechnology, Seoul National University) ;
  • Park, Jae-Young (School of Agricultural Biotechnology, Seoul National University) ;
  • Shim, Hee-Jin (School of Agricultural Biotechnology, Seoul National University) ;
  • Shin, Sang-Chul (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Boo, Kyung-Saeng (School of Agricultural Biotechology, Seoul National University) ;
  • Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2004.03.01
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Abstract

Expression of a fusion protein between B. thuringiensis crystal protein, Cry1Ac1 and a scorpion insect toxin (AaIT, Androctonus australis Hector insect toxin) in acrystalliferous B. thuringiensis strain (Cry-B strain) was examined. The cry 1Ac1 gene was cloned in B. thuringiensis-E coli shuttle vector, pHT3101, under the control of the native cry 1Ac1 gene promoter (pProAc) and a gene encoding AaIT was inserted in XhoI site in the middle of the cry 1Ac1 gene (pProAc-ScoR). B. thuringiensis Cry-B strain carrying pProAc-ScoR (PyoAc-ScoR/CB) produced an inclusion body of irregular shape and the expressed fusion protein is approximately 65 kDa in size. Sporulated cells and spore-crystal mixtures of ProAc-ScoR/CB had insecticidal activity against Plutella xylostella larvae, showing $LT_50$ of ProAc-ScoR/CB (22.59 hrs) lower than that of ProAc/CB (30.06 hrs) at $1{\times}{10^7} {CEU/cm^2}$. These results suggest that the fusion protein including a B. thuringiensis crystal protein and an AaIT may be functionally expressed in B. thupingiensis. Moreover, we verified the additive toxicity of AaIT, which is a new feasible candidate for insect control.

Keywords

References

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