• Korean Journal of Veterinary Research
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• v.31 no.2
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• pp.201-208
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• 1991

In studies on Erysipelothrix rhusiopathiae infection of pigs, a series of investigations were conducted for isolation, biochemical properties, pathogenicity, serotyping and susceptibility to antimicrobial agents. The results could be summarized as follows: 1. A total of 14 isolates of E rhusiopathiae were made from an acute form of swine erysipelas and the incidence was high during the hot summer season. 2. The biochemical properties of the 14 isolates were identical to the reference strain of E rhusiopathiae. 3. Pathogenicity of the isolated strain(89368) were ascertained that $LD_{50}$ for mice was $1.8{\times}10 $ cfu and also typical urticarious lesions and acute septicemia for pigs were induced by intradermal and intravenous inoculation with isolates, respectively. 4. All isolates were serotyped by the agarose gel slide double-diffusion system and proved to be serotype 1. 5. In susceptibility test to antimicrobial agents, 14 isolates of E rhusiopathiae were highly sensitive to ampicillin, baytril, cephalothin, chloramphenicol, penicillin and tetracycline.

• Development and Reproduction
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• v.27 no.2
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• pp.67-75
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• 2023

Polymeric immunoglobulin receptor (pIgR) mediates the transfer of polymeric immunoglobulin to protect organisms and is one of the most important mucosal effectors. In this study, the developmental stage- and tissue-specific expression of pIgR were observed before virus inoculation in olive flounder. pIgR was gradually expressed until the formation of immune tissue, exhibiting high expression in the late juvenile period; thereafter, pIgR expression gradually decreased and exhibited high expression in the spleen and skin. Moreover, pIgR expression after viral hemorrhagic septicemia virus infection was high in the kidney and spleen tissues at high density and low at low density. The results of this study can provide a basis for future studies on breeding density, virus expression, and immune system studies in fish.

• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.79-84
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• 1990

A single inoculation of combined vaccines of Newcastle disease and avian infectious bronchitis of chicken, in a form of gel-oil emulsion type (gel-OEV) was tested their immunogenecity in chickens. The results were summerized as follows: 1. Average minimum and maximum ELISA antibody titers of ND were recorded 2407 and 13144 respectively. In the case of IB, 1824 and 4496 were recorded as minimum and maximum titers. 2. The distribution of average proportional groups, in the lowest and the highest, were 1.6 and 7.0 in ND ELISA and 1.4 and 2.8 in IB ELISA antibody titers. 3. ND ELISA antibody titers were significantly increased upto 7th week after the vaccination. On the other hand, IB ELISA antibody titers were raised upto 4th week after the vaccination.

• Toxicological Research
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• v.8 no.1
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• pp.17-27
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• 1992

Antigenicity of Recombinant Granulocyte macrophage colony stimulating factor(LBD-005), a newly developed drug for hematopoietic growth, was investigated in mice and guinea pigs. 1. Mice showed production of antibodies against LBD-005 (100mg/kg) with alumin]m hydroxide gel(alum) as an adjuvant, judged by the heterologous anaphylaxis(PCA) test using rats. On the other hand, antibodies against ovalbumin(OVA) inoculated with alum were definitely detected. 2. In the studies with guinea pigs, both the inoculation of LBD-005 only and of LBD-005 with complete Freund's adjuvant(CFA) as an adjuvant did not produce positive reactions in homologous passive cutaneous anaphylaxis (PCA).

• Biomedical Science Letters
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• v.16 no.3
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• pp.139-149
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• 2010

The present study was carried out to elucidate whether an environmental strain of Cryptococcus neoformans (environmental C. neoformans) isolated from an environmental source in a park of Busan has an acute pathophysiological effect in rats. On the second day after peritoneal inoculation of environmental C. neoformans, adverse effects occurred from the viewpoint of hematology and biochemistry. Eosinophil damages and crystal formations were found in the blood. Disturbances in cytokines production were observed in the cerebral and pulmonary tissues. Fungal budding existed in the brain, lung, liver and kidney. Tissue injury findings such as inflammation, leukocyte infiltration, bleeding, or degeneration were found in the brain, lung, liver and kidney. The present data suggest that the environmental C. neoformans can cause systematically harmful effects even for short periods of infection (two days of cryptococcal infection) and the adverse effects are summarized as immune derangements and biochemical and/or histological dysfunction and injury on major organ such as the brain, lung, liver and kidney in the immunocompetent hosts. Further studies should be focused on comparing the differences between environmental and clinical strains of C. neoformans.

• Korean Journal of Veterinary Service
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• v.21 no.2
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• pp.141-148
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• 1998

Eighteen strains of Clostpidium perfringens were isolated from the piglets with hemorrhagic enteritis. The characteristics of the outbreaks, clinical signs and lesions were examined. The biochemical properties, type of toxins and susceptibility to antimicrobial agents against the isolates were investigated. 1. The incidence of diarrhea was appeared in 97(22.4%) of 432 piglets examined. 2. The isolation rate of Cl perfingens from the 97 diarrheal faeces were 18.5%(18 strains) 3. The population of Cl perfingens in feces were ranged $10^{8-9}$cfu/g in 5(32.5%) and $10^{3-7}$cfu/g in 13(67.4%) of 18 samples. 4. The toxin type of the 18 isolates investigated by mouse inoculation test was all type C strains of Cl perfringens. 5. As a results of antimicrobial susceptibility test, 18 isolates were higly susceptible to cephalothin, tetracycline and penicillin.

• Journal of the Korean Home Economics Association
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• v.23 no.4
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• pp.69-78
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• 1985

The effects of the garlic preparation on the response of normal and cancer-stricken mice were studied. Food consumption of skin cancer-stricken group decrease more rapidly than that of normal group as cancer of skin develops, but here was no observable change in garlic groups. The rate of weight of increase was the highest in skin cancer-stricken group whereas that of garlic groups was reduced. The feed efficiency ratio was found to be very high in the normal group, whereas that in other goups after inculation of cancer cell was lower. The total protein content of serum was the highest in skin cancer-stricken group was observed to have more conspicuous tumor than other groups, the simultaneous administration of experimental diet and but inoculation of cancer cell produced more severe tumours. It appears that the garlic may exhibit the tendency to reduce the propagation of skin cancer of mice.

• Korean Journal of Veterinary Service
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• v.27 no.1
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• pp.31-39
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• 2004

This study was performed to investigate the rabies surveillance in Gangwon-do from 1997 to 2001. Of 185 laboratory submissions for rabies diagnosis, 65(35.1%) cases were determined to be infected with the rabies virus. Annual incidence of animal rabies was 10 cases in 1997, 20 cases in 1998, 16 cases in 1999, 3 cases in 2000, and 16 cases in 2001, respectively. The total number of the reported cases were increased 382% compared with that of the previous period from 1993 to 1996(7 cases). The relative contributions of infected animals in this period were as follows: cattles(24 cases), dogs(26 cases), and raccoons(15 cases). Rabies outbreak in raccoon were steadily expanded. Epidemiologic surveillance showed the rabies outbreaks spreaded to the southern area in Gangwon-do. All rabies cases were showed positive results to rabies tests such as clinical signs, histopathological findings, indirect flourescent antibody test, and mouse inoculation test. Further studies such as genetic and phylogenetic analysis of the isolates should be performed in order to figure out accurate rabies outbreak.

• Journal of environmental and Sanitary engineering
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• v.17 no.3
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• pp.14-20
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• 2002

In order to improve the quality and sensory evaluation of Fermented Soybean(Chungkugjang meju) were investigated. The samples were prepared and fermented by the inoculation of Bacillus strains (B. subtilis, number 1, B. natto, number 2) that the product made With sanitary canned food. 1. The water content of samples was 46.75~50.60%, pH 5.35~6.95 and total acidity 3.26~3.62. 2. The reducing sugar of content for sample was 9.49~10.05%, Amino-N, 67~396mg% and the activity of protease was 0.36~1.49unit/g. 3. The heavy metals analyzed from sample cans, iron, tin and lead content of sample were 5.32~5.84ppm, 27.31~29.04ppm and 0.019~0.021ppm. 4. Therefore, results that Chungkugjang-meju manufactured from B. natto starter induced better product Quality and sensory test than that of the B. subtilis strain.

• Korean Journal of Veterinary Service
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• v.20 no.4
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• pp.323-329
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• 1997

An outbreak of duck septicemia caused by Pasteurella anatipestifer was studied in Chonnam province. The outbreaks of duck septicemia has become an economically important disease in duck farm. The present study undertaken to identify the etiological agent of duck septicemia and to evaluate the efficacy of bacterin against P anatipestifer in Chonnam province during period from May to December 1996. The results obtained were as follows ; 1. The distribution of etiological agents from collected 328 duck samples were Pasteurella anatipestifer(57%), Escherichia coli(21%), duck viral hepatitis virus(4%), Salmonella sp(1%) and others (10%). 2. The agglutination titers of autogenous vaccine were increased after End vaccination and reached highest at 5-6 weeks post inoculation at the titers of 6.0-4.0.