Fire blight, caused by Erwinia amylovora, is one of the major bacterial disease of apple and pear, causing enormous economic losses worldwide. Several control measures against E. amylovora have been reported till date, however, none of them have proved to be effective significantly against the pathogen. In this study, mechanisms of the copper-based control agents (CBCAs): copper oxychloride (COCHL), copper oxide (COX), copper hydroxide (CHY), copper sulfate basic (CSB), and tribasic copper sulfate (TCS) and their disease severity reduction efficacy against E. amylovora were analyzed. Bis-1,3-dibutylbarbituric acid trimethine oxonol, carboxyl fluorescein diacetate succinimidyl ester, and 5-cyano-2,3-ditolyl tetrazolium chloride staining were used to check the damage of membrane potential, cytoplasmic pHin, and respiration of CBCAs-treated E. amylovora, respectively. High disturbance in the membrane potential of E. amylovora was found under COX and COCHL treatments. Similarly, higher significant changes in the inner cytoplasmic pHin were observed under COX, COCHL, and TCS treatment. CHY and COCHL-treated E. amylovora showed a significant reduction in respiration. In vitro bioassay results revealed that CHY, CSB, and TCS at 2,000 ppm reduced the severity of fire blight both in pre- and post-treatment of CBCAs in immature apple fruits and seedlings. Overall, the most effective CBCAs against E. amylovora could be CHY at 2,000 ppm as its showed inhibition mechanisms and disease severity reduction.
Objective: This experiment was conducted to evaluate the effects of a corn- or cassava- based diet on the production of meat-type ducks. Methods: Four hundred day-old ducks were used in this experiment. They were divided into five groups with each group replicated eight times. The ducks fed the corn-based diets served as the control group. The four other groups comprised different treatments, with each one given the cassava-based diet based on phase-feeding. Three treatments were fed the cassava-based diet from 16, 28, and 35 d; respectively up to 42 d of age and the other group was fed the cassava-based diet from 1 to 42 d of age. Results: The results indicated that ducks on either the corn- or cassava-based diets were similar in growth during 1 to 9 d of age. However, toward 35 to 42 d, the cassava-diet produced a higher weight gain (p<0.05). The cassava-based diet was better than the corn-based diet at increasing the outer and inner breast weights at 28, 35, or 42 d (p<0.05). In contrast, the corn-based diet was better at increasing abdominal fat (p<0.05). The two diets did not differ in their effects on the serum triglyceride, cholesterol, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, very-low-density lipoprotein-cholesterol, and liver cholesterol. The corn-based diet, however, caused a highly significantly greater level of liver triglyceride (p<0.01). Conclusion: The results of this study suggest that both the cassava- and corn- based diets are similar in their effect on meat-type ducks during the starter stage but toward the finisher stage, the cassava-based diet has a better influence on weight gain and carcass characteristics.
Zhang, Wei;Wang, Runlian;Zhu, Xiaoping;Kleemann, David O;Yue, Chungwang;Jia, Zhihai
Asian-Australasian Journal of Animal Sciences
/
v.20
no.12
/
pp.1843-1848
/
2007
Thirty-six 1.5 year-old Inner Mongolian White Cashmere wether goats (body weight $28.14{\pm}1.33$ kg) were used to determine the effects of dietary copper (Cu) concentration on ruminal fermentation, nutrient digestibility and cashmere fibre characteristics. Wethers were fed a basal diet (containing 7.46 mg Cu/kg DM) that was supplemented with either 0 (control), 10, 20 or 30 mg Cu/kg DM. To ensure full consumption, animals were fed restrictedly with 0.75 kg feed (DM) in two equal allotments per day. The results indicated that: (1) supplemental 10 mg Cu/kg DM in the basal diet significantly (p<0.05) decreased ruminal fluid pH value and total VFA concentrations were significantly (p<0.05) increased on all Cu treatment groups. (2) Cu supplementation had no influence on DM intake and digestibility of DM, CP and ADF (p>0.05); however, NDF digestibility of groups supplemented with 10 and 20 mg Cu/kg DM were significantly higher than that of the control group (p<0.05). Apparent absorption and retention of copper were decreased with increasing level of supplementation. (3) 20 mg Cu/kg DM treatment significantly (p<0.05) improved cashmere growth rate, but cashmere diameter was not affected by Cu supplementation (p>0.05). In conclusion, supplementation of cashmere goats with Cu at the rate of 10 to 20 mg/kg DM in the basal diet resulted in some changed rumen fermentation and was beneficial for NDF digestibility, while supplementation of 20 mg Cu/kg DM improved cashmere growth. Collectively, the optimal supplemental Cu level for cashmere goats during the fibre growing period was 20 mg/kg DM (a total dietary Cu level of 27.46 mg/kg DM).
Muthukaruppasamy, S.;Abudhahir, A.;Saravanan, A. Gnana;Gnanavadivel, J.;Duraipandy, P.
Journal of Electrical Engineering and Technology
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v.13
no.5
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pp.1886-1900
/
2018
This paper proposes a confronting feedback control structure and controllers for positive output elementary super lift Luo converters (POESLLCs) working in discontinuous conduction mode (DCM). The POESLLC offers the merits like high voltage transfer gain, good efficiency, and minimized coil current and capacitor voltage ripples. The POESLLC working in DCM holds the value of not having right half pole zero (RHPZ) in their control to output transfer function unlike continuous conduction mode (CCM). Also the DCM bestows superlative dynamic response, eliminates the reverse recovery troubles of diode and retains the stability. The proposed control structure involves two controllers respectively to control the voltage (outer) loop and the current (inner) loop to confront the time-varying ON/OFF characteristics of variable structured systems (VSSs) like POESLLC. This study involves two different combination of feedback controllers viz. the proportional integral controller (PIC) plus sliding mode controller (SMC) and the fuzzy logic controller (FLC) plus SMC. The state space averaging modeling of POESLLC in DCM is reviewed first, then design of PIC, FLC and SMC are detailed. The performance of developed controller combinations is studied at different working states of the POESLLC system by MATLAB-Simulink implementation. Further the experimental corroboration is done through implementation of the developed controllers in PIC 16F877A processor. The prototype uses IRF250 MOSFET, IR2110 driver and UF5408 diodes. The results reassured the proficiency of designed FLC plus SMC combination over its counterpart PIC plus SMC.
Purpose: To evaluate an effect of additional firing process after sintering of monolithic zirconia crown on marginal and internal fit through three-dimensional analysis. Materials and methods: Ten monolithic zirconia crowns were fabricated using titanium abutment model. Monolithic zirconia crowns were designed, milled, and sintered as a control group, and additional firing with coloring was performed as a test group. Three dimensional analysis were performed by using triple-scan protocol, and cross-section analysis on mesio-distal and disto-lingual section was evaluated to measure marginal and internal fitness. Then, three-dimensional surface difference on between two groups was evaluated (${\alpha}=.05$). Results: There was statistically significant difference between the control group ($32.0{\pm}24.3{\mu}m$) and the test group ($17.0{\pm}10.8{\mu}m$) in the mesial axial wall (P < .02) and the control group ($60.2{\pm}24.3{\mu}m$) and the test group ($71.8{\pm}21.5{\mu}m$) in the distal axial wall (P < .01). There was no statistically significant difference at the remaining point. Conclusion: There was no statistical significance on the deviation of inner surface of crown according to firing number, and the results of both group were considered clinically acceptable.
Ordered single-walled hollow aluminogermanate (ALGE) nano-balls(NBs) and nano-tubes(NTs) have been self-assembled from the ALGE precursors having an Al/Ge ratio of 1.33 using simple pH-control. The hollow ALGE NBs with average monodisperse diameters of 5 nm and chemistry of Al/Ge=1.5~1.6 were formed through structural assembly in the ALGE solution (Al/Ge=1.33) highly basified to pH=13(Na/Al=28~30) and followed by immediate acidification to pH=9. When the basified solution(pH=13) were acidified to pH=4, ALGE S-NTs (Short-fiber nano-tubes) with diameters of 3.3 nm, 15~20 nm in length, and chemistry of Al/Ge=2.6~2.8 were successfully synthesized. Whereas the solution was basified to pH=9, and subsequently acidified to pH=4, L-NTs(Long-fiber nano-tubes) with >100 nm in length were synthesized for the first time. The self-assembly of the hollow NBs, S-NTs, and L-NTs form the ALGE precursors can be explained by the degree of $H^+$-dissociation of the -Ge-OH inner surfaces, which was controlled by amount of $Na^+$ and pH conditions of ALGE precursor solutions. This results indicate that target forms of ALGE nanomaterials can be synthesized by simple pH controls.
The purpose of this study is to find out how well the middle school students are practicing the health promotion behaviors and the factors relating their health promotion behavior. Questionnaire survey on 922 middle school students attending 6 middle schools (three middle schools for each sex) located in Taegu City from the 7th through 19th of Feb. 2000 were conducted. The following were as follows; 1. The perceived health status is higher in male students than in female students(p〈0.01). And the ratio of the students' feeling that they are healthy becomes also high in proportion to their economic status, and their mothers' educational level, and their parents' interest in health(p〈0.01). The perceived importance of health is high in proportion to the students' economic status, and their parent's interest in health. 2. In case of the Health Locus of Control in Personality, the students with both parents have higher trend of inner control than the students with single mother or single father. The perceived self efficacy is significantly higher in male students than in female students(p〈0.01). And it becomes significantly high in-proportion to the students' economic status and their parents' educational level and interest in health(p〈0.01). It is also higher in the students who had no diseases. 3. In case of the perceived benefit of the health promotion behavior, the ratio of the students responding that it is high is higher in male students than in female students(p〈0.01). It also becomes high in proportion to the students' economic status, and their parent's educational level and interest in health(p〈0.01). The barriers of the health promotion behavior was found to have no variables that are related to itself. 4. According to the data from Multiple Regression of Analysis which has the health promotion behavior practice as a subordinate variable, in male students' case the degree of health promotion behavior practice becomes high in proportion to their parents' interest in health, and the perceived health status. Their degree of health promotion behavior practice is also in proportion to their perceived self efficacy and the perceived benefit of the health promotion behavior practice. But in case of the barriers of the health promotion behavior practice, the result is the opposite. As to the female students, their health behavior practice becomes high in proportion to their parents' interest in health. It also becomes high in proportion to the perceived health status, the understanding of the importance of the health, the perceived self efficacy and the perceived benefit of the health promotion behavior. But in case of the barriers of the health promotion behavior, it was the same as the male students' case.
Kim, Hye Jin;Lee, Ki Hwan;Park, Sung Baek;Choi, Young Bae;Yang, Jung Bo
Clinical and Experimental Reproductive Medicine
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v.42
no.3
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pp.94-100
/
2015
Objective: The goal of this study was to ascertain optimal assisted hatching (AH) method in frozen embryo transfer. We compared the effect of depending on whether mechanical or laser-AH was performed before or after the vitrification of embryo development rate and blastocyst cell numbers. Methods: In order to induce superovulation, pregnant mare's serum gonadotropin followed by human chorionic gonadotropin were injected into 4- to 5-week-old female mice. 2-cell embryos were then collected by flushing out the oviducts. The Expanded blastocysts were recovered after the collected embryos were incubated for 48 hours, and were then subjected to artificial shrinkage (AS) and cross-mechanical AH (cMAH) or quarter-laser zona thinning-AH (qLZT-AH) were carried out using the expanded blastocysts before or after vitrification. After 48 hours of incubation, followed by vitrification and thawing (V-T), and blastocysts were fluorescence stained and observed. Results: The rate of formation of hatched blastocysts after 24 and 72 hours of incubation was significantly higher in the AS/qLZT-AH/V-T group than in the other groups (p<0.05). The cell number of the inner cell mass was higher in AS/V-T/non-AH and AS/V-T/cMAH groups than those of others (p<0.05). In the control group, the number of trophectoderm and the total cell number were higher than in the AS-AH group (p<0.05). Conclusion: The above results suggest that AS and AH in vitrification of expanded blastocysts lead to the more efficient formation of hatched blastocysts in mice.
Background: Idiopathic sudden sensorineural hearing loss(ISSHL) has been generally defined as an abrupt loss of auditory acuity without precipitating factors. Since ISSHL was first described as a disease entity, numerous reports assessing a variety of treatment regimens have been published. But its etiology, pathogenesis, treatment, and prognosis are controversial at the present time. Stellate ganglion block(SGB) has been used for the treatment of ISSHL by vasodilatation and increased blood flow to the inner ear. Methods: We reviewed the records of 152 patients to investigate the efficacy of SGB for ISSHL. The control group was managed with medications such as hypaque, heparin, steroid, nicotinic acid, and vitamins. The SGB group was managed with SGB and the same medications. SGB was performed with 8 ml of 1.0% mepivacaine. The efficacy of treatment was evaluated by pure-tone average following therapy. The recovery of hearing was defined as Siegel's criteria I, II, or III. Results: The recovery rate of the SGB group was higher than that of the control group(61.5 vs. 42.9%, p<0.05). The recovery rate was higher in patients who were treated early, within 7 days from the onset of symptoms, especially in the SGB group(79.7%). And in the case that initial hearing loss was severe (>70dB), the SGB group had a higher recovery rate(64.2%) than the control group(42.5%). Conclusions: SGB is thought to be a useful therapy for ISSHL, especially in the patients who were treated soon after onset or whose initial hearing loss was severe.
This study was carried out to investigate the quality characteristics and antioxidative activity of bread containing lotus leaf powder (in ratios of 0, 1, 2, and 3% of the total flour). The pH of dough and bread were decreased as the concentration of lotus leaf powder increased. The volume of dough during fermentation did not show significant difference between 1% lotus leaf powder containing dough and control. The weight of bread increased by addition of lotus leaf powder. The volume, specific volume and baking loss rate of the bread decreased as lotus leaf powder levels increased. Redness and yellowness of the inner crumb were decreased by the addition of lotus leaf powder, but lightness was increased. 2,2-diphenyl-1-picrylhydrazyl-radical scavenging activity and total polyphenol contents were increased significantly as the concentration of lotus leaf powder increased. The overall acceptability of bread containing 1% lotus leaf powder showed no significant difference compared with control. The result exhibited that adding the lotus leaf powder into the bread increased antioxidant activity, and the highest quality improvement was obtained by incorporating 1% (w/w) lotus leaf powder into the bread formula.
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