• Journal of Institute of Control, Robotics and Systems
• /
• v.12 no.12
• /
• pp.1173-1177
• /
• 2006

Using an inner P controller, a tuning rule useful for processes with wide ratios of time delay over time constant is proposed. Internal model control method and pole assignment method are utilized. It can be used for processes with wide range of the ratio of time delay to time constant without incovenience to choose different tuning rules.

• Animal Bioscience
• /
• v.35 no.7
• /
• pp.1010-1020
• /
• 2022

Objective: The experiment was conducted to evaluate the effects of maternal undernutrition during late pregnancy on the expressions of genes involved in growth and development in ovine fetal perirenal brown adipose tissue (BAT). Methods: Eighteen ewes with singleton fetuses were allocated to three groups at day 90 of pregnancy: restricted group 1 (RG1, 0.33 MJ metabolisable energy [ME]/kg body weight [BW]^0.75/d, n = 6), restricted group 2 (RG2, 0.18 MJ ME/kg BW^0.75/d, n = 6), and a control group (CG, ad libitum, 0.67 MJ ME/kg BW^0.75/d, n = 6). The fetuses were removed at day 140 of pregnancy. All data were analyzed by using the analysis of variance procedure. Results: The perirenal fat weight (p = 0.0077) and perirenal fat growth rate (p = 0.0074) were reduced in RG2 compared to CG. In fetal perirenal BAT, the protein level of uncoupling protein 1 (UCP1) (p = 0.0001) was lower in RG1 and RG2 compared with CG and UCP1 mRNA expression (p = 0.0265) was decreased in RG2. The protein level of myogenic factor 5 (Myf5) was also decreased in RG2 (p = 0.0001). In addition, mRNA expressions of CyclinA (p = 0.0109), CyclinB (p = 0.0019), CyclinD (p = 0.0015), cyclin-dependent kinase 1 (CDK1) (p = 0.0001), E2F transcription factor 1 (E2F1) (p = 0.0323), E2F4 (p = 0.0101), and E2F5 (p = 0.0018) were lower in RG1 and RG2. There were decreased protein expression of peroxisome proliferator-activated receptor-γ (PPARγ) (p = 0.0043) and mRNA expression of CCAAT/enhancer-binding protein-α (C/EBPα) (p = 0.0307) in RG2 and decreased PPARγ mRNA expression (p = 0.0008) and C/EBPα protein expression (p = 0.0015) in both RG2 and RG1. Furthermore, mRNA expression of bone morphogenetic protein 4 (BMP4) (p = 0.0083) and BMP7 (p = 0.0330) decreased in RG2 and peroxisome proliferator-activated receptor co-activator-1α (PGC-1α) reduced in RG2 and RG1. Conclusion: Our observations support that repression of regulatory factors promoting differentiation and development results in the inhibition of BAT maturation in fetal perirenal fat during late pregnancy with maternal undernutrition.

• Animal Bioscience
• /
• v.35 no.9
• /
• pp.1351-1359
• /
• 2022

Objective: The objective of this study was to analyse the differentially abundant proteins caused by freeze-thawing of ram sperm and explore candidate proteins of interest for their ability to improve ram sperm cryopreservation outcomes in vitro. Methods: Sperm were from three mature Dorper. Fresh and frozen sperm proteins were extracted, and the differentially abundant proteins were analysed by mass spectrometry. Among these proteins, lactoferrin (LTF) was selected to be added before cryopreservation. Next, sperm samples were diluted in Tris extender, with the addition of 0, 10, 100, 500, and 1,000 ㎍/mL of LTF. After thawing, sperm quality was evaluated by motility, plasma membrane integrity, mitochondrial activity and reactive oxygen species (ROS). Results: Cryopreservation significantly altered the abundance of 40 proteins; the abundance of 16 proteins was increased, while that of 24 proteins was decreased. Next, LTF was added to Tris extender applied to ram sperm. The results showed that sperm motility and plasma membrane integrity were significantly improved (p<0.05) by supplementation with 10 ㎍/mL LTF compared to those in the control group. There was no significant difference in mitochondrial activity between the 0 ㎍/mL group and other groups (p>0.05). Supplementation of the cryoprotective extender with 10 ㎍/mL LTF led to decreased ROS levels compared with those in the control and other groups (p<0.05). Conclusion: The LTF is an important protein during cryopreservation, and the addition of 10 ㎍/mL LTF to a cryoprotective extender can significantly improve the function of frozen ram sperm.

• Animal Bioscience
• /
• v.35 no.7
• /
• pp.989-998
• /
• 2022

Objective: This study investigated the effects of intrauterine growth restriction (IUGR) during late pregnancy on the cell growth, proliferation, and differentiation in ovine fetal thymuses. Methods: Eighteen time-mated Mongolian ewes with singleton fetuses were allocated to three groups at d 90 of pregnancy: restricted group 1 (RG1, 0.18 MJ ME/body weight [BW]^0.75/d, n = 6), restricted group 2 (RG2, 0.33 MJ ME/BW^0.75/d, n = 6) and control group (CG, ad libitum, 0.67 MJ ME/BW^0.75/d, n = 6). Fetuses were recovered at slaughter on d 140. Results: The G0/G1 phase cell number in fetal thymus of the RG1 group was increased but the proliferation index and the expression of proliferating cell nuclear antigen (PCNA) were reduced compared with the CG group (p<0.05). Fetuses in the RG1 group exhibited decreased growth hormone receptor (GHR), insulin-like growth factor 2 receptor (IGF-2R), and their mRNA expressions (p<0.05). For the RG2 fetuses, there were no differences in the proliferation index and PCNA expression (p>0.05), but growth hormone (GH) and the mRNA expression of GHR were lower than those of the CG group (p<0.05). The thymic mRNA expressions of cyclin-dependent protein kinases (CDKs including CDK1, CDK2, and CDK4), CCNE, E2-factors (E2F1, E2F2, and E2F5) were reduced in the RG1 and RG2 groups (p<0.05), and decreased mRNA expressions of E2F4, CCNA, CCNB, and CCND were occurred in the RG1 fetuses (p<0.05). The decreased E-cadherin (E-cad) as a marker for epithelial-mesenchymal transition (EMT) was found in the RG1 and RG2 groups (p<0.05), but the OB-cadherin which is a marker for activated fibroblasts was increased in fetal thymus of the RG1 group (p<0.05). Conclusion: These results indicate that weakened GH/IGF signaling system repressed the cell cycle progression in G0/G1 phase in IUGR fetal thymus, but the switch from reduced E-cad to increased OB-cadherin suggests that transdifferentiation process of EMT associated with fibrogenesis was strengthened. The impaired cell growth, retarded proliferation and modified differentiation were responsible for impaired maturation of IUGR fetal thymus.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.5
• /
• pp.698-704
• /
• 2014

This study was conducted to investigate the effects of chestnut inner shell powder on antioxidant activities and quality characteristics of pork patties. Patties were prepared with different amounts of chestnut inner shell powder (0, 1, 3, and 5% of pork quantity). The total phenol contents and DPPH free radical scavenging activities of the patties significantly increased with increasing amount of chestnut inner shell powder. The moisture content and pH of row patties were not significantly different between the control group and treatment groups. The cooking loss rate significantly decreased with increasing chestnut inner shell powder amount. Reduction ratio of the diameter of experimental patties was lower than that of the control patties, although it was not significant. As the content of chestnut inner shell powder increased, L-value decreased while a-value increased in both row patties and cooked patties. However, b-value changed upon heating. The b-value of row patties also increased with increasing chestnut inner shell powder content, whereas the b-value of cooked patties decreased. The hardness, chewiness, gumminess, and cohesiveness of the control group were lower than those of the treatment group. The consumer acceptability score for patties with 1% chestnut inner shell powder was higher than that for patties in terms of overall preference, appearance, color, and texture. Further, overall preference, appearance, and color for patties with chestnut inner shell powder 3% were higher than those of the control group. We suggest that chestnut inner shell can be improve the quality characteristics and consumer acceptability of pork patties, as well as the development of health-oriented meat products.

• Parasites, Hosts and Diseases
• /
• v.57 no.2
• /
• pp.93-99
• /
• 2019

Both Plasmodium spp. and Toxoplasma gondii are important apicomplexan parasites, which infect humans worldwide. Genetic analyses have revealed that 33% of amino acid sequences of inner membrane complex from the malaria parasite Plasmodium berghei is similar to that of Toxoplasma gondii. Inner membrane complex is known to be involved in cell invasion and replication. In this study, we investigated the resistance against T. gondii (ME49) infection induced by previously infected P. berghei (ANKA) in mice. Levels of T. gondii-specific IgG, IgG1, IgG2a, and IgG2b antibody responses, $CD4^+$ and $CD8^+$ T cell populations were found higher in the mice infected with P. berghei (ANKA) and challenged with T. gondii (ME49) compared to that in control mice infected with T. gondii alone (ME49). P. berghei (ANKA) + T. gondii (ME49) group showed significantly reduced the number and size of T. gondii (ME49) cysts in the brains of mice, resulting in lower body weight loss compared to ME49 control group. These results indicate that previous exposure to P. berghei (ANKA) induce resistance to subsequent T. gondii (ME49) infection.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.2
• /
• pp.207-212
• /
• 2012

The effects of sheep urine and dung patches on methane ($CH_4$) and carbon dioxide ($CO_2$) fluxes were investigated during the summer-autumn in 2010, to evaluate their contribution to climate change in a desert grassland in Inner Mongolia, China. Results indicate that the cumulative $CH_4$ emissions for dung patches, urine patches and control plots were -0.076, -0.084, and -0.114 g/$m^2$ and these were net $CH_4$ sinks during the measured period. The level of $CH_4$ intake from urine and dung plots decreased 25.7%, and 33.3%, respectively, compared with a control plot. $CO_2$ fluxes differed (p<0.01) in urine plots, with an average of 569.20 mg/$m^2$/h compared with control plots (357.62 mg/$m^2$/h) across all sampling days. Dung patches have cumulative $CO_2$ emissions that were 15.9% higher compared with the control during the 55-d period. Overall, sheep excrement weakened $CH_4$ intake and increased $CO_2$ emissions.

• 제어로봇시스템학회:학술대회논문집
• /
• 2004.08a
• /
• pp.444-447
• /
• 2004

A new approach to compute the control moves in Extended Dynamic Matrix Control (EDMC) is presented. In this approach, the number of variables, determined in the inner loop of the control algorithm using iterative methods, is reduced from P , the prediction horizon to M , the control horizon. Since M is usually much smaller than P , this modifies the control algorithm from computational point of view. To justify the modification, the computational requirements are compared to those of the existing EDMC algorithm.

• 제어로봇시스템학회:학술대회논문집
• /
• 2000.10a
• /
• pp.466-466
• /
• 2000

This paper presents the sinusoidal current ministep technique to drive stepping motor. The stepping motor is coupling to the increment encoder to detect the position and speed of the stepping motor. The data from the encoder is decoded to sine and cosine signal and fed to the driver system. The driver system has two loops control, the inner loop and the outer loop. The inner loop is used to control the rotating of the stepping motor and the outer is used to control the speed of the motor. The rotating of the stepping motor is controlled with the sinusoidal signal. The test results of the inner loop control can control the revolution of the stepping motor is smooth and continuously with similar to the DC motor. The outer loop uses to control the speed of the stepping motor with control the DC voltages apply to the driver. The DC voltages that apply to the driver is controlled by the AC-DC converter The test results of the outer loop control, it can control the speed of motor which is provide the any load in the design.

• Proceedings of the KIPE Conference
• /
• 2001.10a
• /
• pp.184-188
• /
• 2001

In this paper, a new fully digital control method for single-phase UPS inverter, which is based on the double control loop such as the outer voltage control loop and inner current control loop, is proposed. The inner current control loop is designed and implemented in the form of internal model control and takes the presence of computational time-delay into account. Therefore, this method provides an overshoot-free reference-to-output response. In the proposed scheme, the outer voltage control loop employing P controller with resonance model implemented by a DSP is introduced. The proposed resonance model has an infinite gain at resonant frequency, and it exhibits a function similar to an integrator for AC component. Thus the outer voltage control loop causes no steady state error as regard to both magnitude and phase. The effectiveness of the proposed control system has been demonstrated by the simulation and experimental results respectively.