• Title/Summary/Keyword: infection method

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A study to identify an occupational hazards for hospital working health care providers from needle stick injury as an occupational hazards of health care providers in hospital and prevention (의료요원의 주사바늘 상해 실태와 예방대책을 위한 일 연구)

  • Han, Jung-Suk;Kang, Kyu-Sook;Kim, Hea-Sook
    • The Korean Nurse
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    • v.34 no.2
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    • pp.45-57
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    • 1995
  • A study .to identify an occupational hazards for hospital working health care providers from needle stick injury as an occupational hazards of health care providers in hospital and prevention A survey of 2430 health professionals (2184 nurses, 182 doctors, and 64 Lab technicians) was conducted to describe and provide information about 1) the experience of needle stick. 2) the number of needle stick, 3) the treatment after needle stick. 3) the situation of needle stick, 4) the report of needle stick, 5) the cause of needle stick, 6) the discard method of used needles, and 7) how to worry about getting infection disease after needle stick. Data were collected using questionnarires constructed by the authors and tested by a pilot study. Results of the study showed that 96.7% of the sample had an experience of needle stick (96.8% of the nurses, 96.7% of the doctors, and 92.2% of Lab technicians). Seventy seven percent of the sample experienced less than 10 needle sticks, 19% of the sample experienced 11 to 20 needle sticks, and the rest of the sample experienced more than 20 needle sticks. The situations where needle sticks occurred include intrvenous injection (36.5%), intramuscular injection (21.6%), blood withdraw (17.8%), and preparation(11.8%). The study showed that needle sticks (67.5%) usually occurred after client treatment. Health professionals used recapping method (55%) after they used needles. Needle sticks were predominantly caused by the carelessness of health professionals (61%), After needle sticks, 88.2% of the sample subjects treated needle sticks using disinfection technique by themselves. Most of health professionals (92.6%) did not report the accident. and 95.6% of them did not receive any test or further treatment. After needle sticks, 87.8% of nurses, 83.6% of doctors, and 96.6% of lab technicians worried about hepatitis infection. 'Health professionals also worried about AIDS infection, tetanus, venereal infection, and skin injury. These findings suggest that health professionals are at high-risk of needle stick and fail to report needle stick accidents. They should pay more attention to needle stick in order to avoid unwanted infection.

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Prediction of HIV and AIDS Incidence Using a Back-calculation Model in Korea (후향연산 모형 (Back-calculation model)을 이용한 국내 HIV 감염자와 AIDS 환자의 추계)

  • Lee, Ju-Young;Goh, Un-Yeong;Kee, Mee-Kyung;Kim, Jee-Yun;Hwang, Jin-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.35 no.1
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    • pp.65-71
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    • 2002
  • Objective : To estimate the status of HIV infection and AIDS incidence using a back-calculation model in Korea. Methods : Back-calculation is a method for estimating the past infection rate using AIDS incidence data. The method has been useful for obtaining short-term projections of AIDS incidence and estimating previous HIV prevalence. If the density of the incubation periods is known, together with the AIDS incidence, we can estimate historical HIV infections and forecast AIDS incidence in any time period up to time t. In this paper, we estimated the number of HIV infections and AIDS incidence according to the distribution of various incubation periods Results : The cumulative numbers of HIV infection from 1991 to 1996 were $708{\sim}1,426$ in Weibull distribution and $918{\sim}1,980$ in Gamma distribution. The projected AIDS incidence in 1997 was $16{\sim}25$ in Weibull distribution and $13{\sim}26$ in Gamma distribution. Conclusions : The estimated cumulative HIV infections from 1991 to 1996 were $1.4{\sim}4.0$ times more than notified cumulative HIV infections. Additionally, the projected AIDS incidence in 1997 was less than the notified AIDS cases. The reason for this underestimation derives from the very low level of HIV prevalence in Korea, further research is required for the distribution of the incubation period of HIV infection in Korea, particularly for the effects of combination treatments.

Detection of Dirofilaria immitis by Reverse Transcription Polymerase Chain Reaction in Canine (역전사중합효소연쇄반응을 이용한 개심장사상충의 검출)

  • 이영준;박진호;권오덕;이주목
    • Journal of Veterinary Clinics
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    • v.16 no.1
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    • pp.177-181
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    • 1999
  • This study was undertaken to clarify the more accurate detecting method of Dirofilaria immitis. Seven dogs, average 7.47 years old, confirmed with Dirofilaria immitis infection by modified Knott's method were used as the experimental animals. cDNA was constructed using oligodT(15) primer after extracting total RNA from the blood of dogs that were confirmed with Dirofilaria immitis infection. As a result of polymerase chain reaction with template using constructed cDNA, the predicted products of a 378 base-pair DNA fragment was amplified. From these results, RT-PCR was more sensitive and effective than modified Knott's method to detect Dirofilaria immitis in dogs.

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Development of an Efficient Mechanical Inoculation Technique to Screen Barley Genotypes for Resistance to Barley mild mosaic virus Disease and its Comparison to Natural Infection

  • Jonson, Gilda;Park, Jong-Chul;Noh, Tae-Hwan;Kim, Mi-Jung;Hyun, Jong-Nae;Kim, Jong-Gon
    • The Plant Pathology Journal
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    • v.22 no.4
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    • pp.348-352
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    • 2006
  • Barley mild mosaic virus(BaMMV) is a soilborne Bymovirus vectored by root-infecting fungus, Polymyxa graminis. Mechanism of cultivar's resistance to BaMMV in field tests are difficult to assess since resistance could be either due to the virus or to P. graminis, or both. Whereas, available mechanical inoculation methods for BaMMV and other related viruses are labor intensive, give inconsistent results and generally result in low infection rates. Inoculation method using stick with gauze(SWG) was developed for BaMMV. The improved method proved to be simple, efficient, and reliable. The infected leaf tissues were preserved by drying in a frozen state under high vaccum(freeze dried barley infected leaves) to circumvent reduction of virus infectivity during storage. Five Korean barley cultivars were mechanically inoculated with BaMMV-infected sap by the improved method. Infection rates obtained were compared with natural infection. Cultivar Naehanssalbori showed resistance to BaMMV in the field trials but was found highly susceptible in the greenhouse tests by mechanical inoculation, indicating that the field resistance may be possibly due to resistance to P. graminis.

Status of Nosocomial Urinary Tract Infections in the ICU: Molecular Epidemiology of Imipenem Resistant P. aeruginosa (중환자실내 병원성 요로감염 실태와 전파경로: Imipenem Resistant P. aeruginosa[IRPA]의 분자역학적 특성을 중심으로)

  • Yu, Seong-Mi;Jeon, Seong-Sook;Kang, In-Soon;An, Hye-Gyung
    • Journal of Korean Academy of Nursing
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    • v.36 no.7
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    • pp.1204-1214
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    • 2006
  • Purpose: This retrospective study was done to evaluate the status of nosocomial urinary tract infections and to determine the risk factors and transmission route of causal IRPA through molecular epidemiology. Method: Two hundred ninety-nine of 423 patients admitted to the internal medicine and surgery ICU at a university hospital incity B had a positiveurine culture. Twelve of the 299 patients who had a urinary tract infection had IRPA strains. The data was collected from November 1, 2004 to January 31, 2005. The following results were obtained after the data was analyzed using percentile and UPGMA. Result: The rate of nosocomial urinary tract infections in the ICU was 10.8%. Therewere 16.8 cases of infection based on the period of hospitalization. There were 16.9 cases of infection based on the use of a foley catheter. The rate of nosocomial urinary tract infection in the ICU and urinary tract infections related to IRPA were higher in patients with the following characteristics: men, old age, admission through the emergency room, longer than seven days admission, severity of admitting causes, disturbance of consciousness, hydration less than 300cc in 24hours, a long course of antibiotics, a long period of foley catheterization and perineal care. Most of the microorganisms that caused the urinary tract infection were gram negative bacilli, among which P. aeruginosa was found in 70 patients (18.5%) and IRPA in 12 (4.0%). Among the 12 IRPA strains that were tested with PFGE, eight showed a dice coefficient higher than 80%, suggesting a genetic relationship. They were related with the period of hospitalization in the same ICU. These patients all received direct care for a urinary tract infection. Conclusion: Through these results, IRPA can be consideredas a contributing factors to urinary tract infections thus, active preventative measures are needed by the medical staff.

Predictors of Blood and Body Fluid Exposure and Mediating Effects of Infection Prevention Behavior in Shift-Working Nurses: Application of Analysis Method for Zero-Inflated Count Data (교대근무 간호사의 혈액과 체액 노출 사고 예측 요인과 감염예방행위의 매개효과: 영과잉 가산 자료 분석방법을 적용하여)

  • Ryu, Jae Geum;Choi-Kwon, Smi
    • Journal of Korean Academy of Nursing
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    • v.50 no.5
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    • pp.658-670
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    • 2020
  • Purpose: This study aimed to identify the predictors of blood and body fluid exposure (BBFE) in multifaceted individual (sleep disturbance and fatigue), occupational (occupational stress), and organizational (hospital safety climate) factors, as well as infection prevention behavior. We also aimed to test the mediating effect of infection prevention behavior in relation to multifaceted factors and the frequency of BBFE. Methods: This study was based on a secondary data analysis, using data of 246 nurses from the Shift Work Nurses' Health and Turnover study. Based on the characteristics of zero-inflated and over-dispersed count data of frequencies of BBFE, the data were analyzed to calculate zero-inflated negative binomial regression within a generalized linear model and to test the mediating effect using SPSS 25.0, Stata 14.1, and PROCESS macro. Results: We found that the frequency of BBFE increased in subjects with disturbed sleep (IRR = 1.87, p = .049), and the probability of non-BBFE increased in subjects showing higher infection prevention behavior (IRR = 15.05, p = .006) and a hospital safety climate (IRR = 28.46, p = .018). We also found that infection prevention behavior had mediating effects on the occupational stress-BBFE and hospital safety climate-BBFE relationships. Conclusion: Sleep disturbance is an important risk factor related to frequency of BBFE, whereas preventive factors are infection prevention behavior and hospital safety climate. We suggest individual and systemic efforts to improve sleep, occupational stress, and hospital safety climate to prevent BBFE occurrence.

Genotype Distribution and Behavioral Risk Factor Analysis of Human Papillomavirus Infection in Uyghur Women

  • Sui, Shuang;Jiao, Zhen;Niyazi, Mayinuer;Sulaiya, Sulaiya;Lu, Ping;Qiao, You-Lin
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.10
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    • pp.5861-5865
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    • 2013
  • We investigated the distribution of HPV genotypes in Uyghur women in Xinjiang region of China, and behavioral factors which could predispose them to HPV infection. In this cross-sectional study, women aged 15-59 years were recruited by cluster sampling method in Yutian region in 2009. Liquid-based cytology samples were analyzed centrally for HPV genotype with a linear array detector. Univariate and multivariate logistic regression analyses were performed to identify behavioral risk factors for HPV infection. A total of 883 Uyghur women were recruited successfully. The prevalence of high-risk HPV and low-risk HPV were 7.25% and 1.58%, respectively; the most common HPVs were HPV16, 51, 31, 39 and 58. We found that age of first sexual intercourse was a strong predictor for HPV infection (odds ratio of 4.01 for ${\leq}15$ years versus ${\geq}25$). Having sexual partners ${\geq}3$ was the second predictor (OR 3.69, 95% CI 2.24-7.16). Cleaning the vagina after sex showed an increased risk of HPV infection (OR 2.72; 95% CI 1.98-5.13); Using the condom showed protective factors for HPV infection (OR 0.36; 95%CI0.12-0.53). HPV16, 51, 31, 39 and 58 were the priority types; the age of first sexual intercourse was identified as a major risk factor for HPV infection. Other notable risks were number of sexual partners and cleaning the vagina after sex. Changing these behavioral risk factors could help to reduce the occurrence of cervical cancer in this population.

Elective tracheostomy scoring system for severe oral disease patients

  • Kim, Yong-Hwan;Kim, Moon-Young;Kim, Chul-Hwan
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.40 no.5
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    • pp.211-219
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    • 2014
  • Objectives: The purpose of this research was to create a scoring system that provides comprehensive assessment of patients with oromaxillofacial cancer or odontogenic infection, and to statistically reevaluate the results in order to provide specific criteria for elective tracheostomy. Materials and Methods: All patients that had oral cancer surgery (group A) or odontogenic infection surgery (group B) during a period of 10 years (2003 to 2013) were subgrouped according to whether or not the patient received a tracheostomy. After a random sampling (group A: total of 56, group B: total of 60), evaulation procedures were observed based on the group classifications. For group A, four factors were evaluated: TNM stage, reconstruction methods, presence of pathologic findings on chest posterior-anterior (PA), and the number of systemic diseases. Scores were given to each item based on the scoring system suggested in this research and the scores were added together. Similarly, the sum score of group B was counted using 5 categories, including infection site, C-reactive protein level on first visit, age, presence of pathologic findings on chest PA, and number of systemic diseases. Results: The scoring system rendered from this research shows that there is a high correlation between the scores and TNM stage in oral cancer patients, or infection sites in odontogenic infection patients. However, no correlation between pathologic findings on chest PA could be found in either group. The results also indicated that for both groups, the hospital day increased with the tracheostomy score. The tracheostomy score cutoff value was 5 in oral cancer patients and 6 in odontogenic infection patients which was used for elective tracheostomy indication. Conclusion: The elective tracheostomy score system suggested by this research is a method that considers both the surgical and general conditions of the patient, and can be very useful for managing patients with severe oral disease.

Monitoring of emaciation disease in cultured Paralichthys olivaceus of Jeju island during 2014-2015

  • Kim, Seung Min;Jun, Lyu Jin;Lee, Da Won;Park, Hyun Kyung;Jeong, Hyun Do;Kim, Jong Sung;Jeong, Joon Bum
    • Fisheries and Aquatic Sciences
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    • v.21 no.6
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    • pp.17.1-17.7
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    • 2018
  • This study investigated the trend in emaciation infection outbreak in olive flounder (Paralichthys olivaceus) of Jeju island, South Korea, during 2014-2015. A total of 900 fish were systematically examined by PCR method using the EM-F/EM-R primer set in April, May, September, November, and December 2014, and the infection rate was recorded. In 2015, the same examination was conducted in March, May, July, and October but with 660 fish. It was found that the infection rate was 18.3~71.6% in 2014, which increased to 16.3~90.3% in 2015. Furthermore, September and December in 2014 and March, July, and October in 2015 showed a relatively higher infection rate. According to the infection trend analysis, which depended on the sample size, the infection occurred in all of fish sizes in this study and 11~30 cm fish group showed the highest infection rate. Histological examination confirmed that the kidney areas of the emaciating infected olive flounder contained several spores of $4{\sim}9{\mu}m$, and in severe cases, the elimination and destruction of tissue were confirmed by PCR. Thus, an important portion of farmed olive flounders in the Jeju region suffers from emaciation disease. This epidemiological survey serves as a useful reference on the emaciation disease of cultured olive flounders in Jeju

Seroprevalence of Mycoplasma hyopneumoniae infection in Korean swine herds (우리나라 양돈장에서 Mycoplasma hyopneumoniae 유병율 조사)

  • Kim, Hye-kwon;Kim, Tae-yung;Lim, Jong-sung;Lee, Yang-ho;Park, Bong-kyun
    • Korean Journal of Veterinary Research
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    • v.45 no.1
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    • pp.55-61
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    • 2005
  • Serum samples of 1,175 pigs from 148 Korean swine farms not using Mycoplasma hyopneumoniae (M. hyo) vaccines were collected for seroepidemiological study of M. hyo infection by indirect ELISA method. Informations of each farm were provided about province where the farm was located and season when blood samples were collected. Then, the selected farms were divided into farm units which had 5 serum samples according to production stages : sow, suckling piglet (<30 days old), nursery pig (30-70 days old), and growing pig (>70 days old). Seroprevalence of M. hyo infection according to production stages, province, and season was investigated by using ELISA-positve rate of the selected samples for each study. This study showed that 85.34% (78.94-91.78%, 95% CI) of farms were positive to M. hyo infection and 34.81% (32.09-37.53%, 95% CI) among pigs were sero-positive to M. hyo infection in Korean swine farms. In the study of seroprevalence by production stage, most farms had sows and growing pigs which were sero-positive to M. hyo infection (sow: 83.05%, growing pigs: 87.72%) and most pigs seemed to be naturally infected by M. hyo at 8-10 weeks of age. Also, M. hyo infection showed seasonal pattern that most pigs were infected in late fall to early winter. However, in the study of seroprevalence by province, there was no significant correlation between province and M. hyo sero-positive rate.