• Journal of Microbiology and Biotechnology
• /
• 제33권9호
• /
• pp.1170-1178
• /
• 2023

Food allergy represents a severe problem for many societies, including sensitive populations, academies, health authorities, and the food industry. Peanut allergy occupies a special place in the food allergy spectrum. To prevent consumption by consumers suffering from a peanut allergy, a rapid and sensitive detection method is essential to identify unintended peanut adulteration in processed foods. In this study, we produced four monoclonal antibodies (MAbs; RO 3A1-12, PB 4C12-10, PB 5F9-23, and PB 6G4-30) specific to thermo-stable and soluble proteins (TSSPs) of peanut and developed an enzyme-linked immunosorbent assay (ELISA) based on the MAbs. Among them, PB 5F9-23 MAb was firmly bound to Ara h 1, and other MAbs strongly reacted to Ara h 3 in the Western blot analysis. An antibody cocktail solution of the MAbs was used to enhance the sensitivity of an indirect ELISA, and the limit of detection of the indirect ELISA based on the antibody cocktail solution was 1 ng/ml and improved compared to the indirect ELISA based on the single MAb (11 ng/ml). The cross-reaction analysis revealed the high specificity of developed MAbs to peanut TSSPs without cross-reaction to other food allergens, including nuts. Subsequently, analyzing processed foods by indirect ELISA, all foods labeled as containing peanuts in the product description were confirmed to be positive. The results indicate that the developed antibodies exhibit high specificity and sensitivity to peanuts and can be used as bio-receptors in immunoassays or biosensors to detect intentional or unintentional adulteration of peanuts in processed foods, particularly heat-processed foods.

• 대한가정학회지
• /
• 제36권5호
• /
• pp.29-46
• /
• 1998

This study investigates how the consumers utilize the elements of information search and how consumers are satisfied with them. For this study, drives who have bought their own cars in two years were chosen for subjects of investigation. And samples were interviewed with an organized questionaire from July 8 to July 27, 1996. 300 samples were used for the final analysis. The major results from this study are as follows. 1) In general information search, consumers get a great deal of information from a talk their friends, neighbors, or colleagues, and they obtain information from an experienced person or a professional. In using sources of information search, they made the best use of their experiences, which is the sources of internal information, and for the second, they used advertising and the explanation of a salesman. In using the contents of information search, they made the best use of information about the merits and demerits in each attribute. In using the method of information search, the mixed search 1 method was used the most, and the attribute search method was used. 2) The casual relationship between the general satisfaction for the products and the relative variable was investigated. An age, the time pressure, the information of the products had a direct and indirect influence on the general for the products. The perceived risk, the number of inquiring activity, a shop and the buying method had a direct influence on the general for the products. An indirect influence was showed in the general satisfaction for the products through the medium of inquiring activity, a shop, and buying method.

• 정보처리학회논문지D
• /
• 제14D권4호
• /
• pp.363-372
• /
• 2007

100여 년 동안 사용자 확인의 한 축을 담당해오던 비밀번호가 비대면 거래가 활성화 되면서 점차 제 기능을 다하지 못하여 이제 바뀔 때가 되었으며, 그 한 방편으로써 간접패스워드인 브레인키를 패스워드시스템에 적용하면 사용자의 패스워드의 해킹 등 다양한 유출 경로가 없으므로, 보다 안전한 전자 상거래를 활성화시킬 수 있다. 비밀번호는 현대인에게 있어서 생활필수품이므로 이에 대한 독점적 지위와 시장선점은 중요한 의미를 갖는다. 또한 비밀번호에 대한 문제는 국내에 국한 되지 않으므로 국내의 상업성은 일개 기업에 국한되지 않고 국제표준화를 선도할 수 있으므로 국가적 이익에도 부합된다. 본 연구에서는 전자상거래에서 발생하는 보안 솔루션을 제시하고자 한다.

• 산업공학
• /
• 제8권3호
• /
• pp.241-248
• /
• 1995

Fa SI(Sysrem Intergration)분야에 있어서 PLC(Programmable Logic Controller)와 컴퓨터간의 접속은 필수 불가결한 요소기술로 자리잡고 있으며, 이러한 기술변화에 맞춰서 기존 유니트의 기술적 발전뿐 아니라 타 유니트와 접속등의 네트워킹에 관련된 내용이 하드웨어, 소트프웨어적으로 발전되고 있는 추세이다. 시스템통합을 하기 위해서는 기본적으로 PLC Networking을 하드웨어, 소프트웨어적으로 수행하여야 하나, 많은 연구들이 PLC 통신 유니트의 기술적 향상 및 표준화에 대한 부분으로 되어왔었다. 본 논문은 정보시스템을 구축할 때 PLC에서 처리하여야 하는 데이터, 혹은 컴퓨터와 송수신 받아야 하는 자료들에 대한 내용과 이들 자료를 PLC 내부에서 처리하는 방법론에 대해 기술코자 한다. 일반적인 Interface 방법으로 접점연결(Point to Point Connection)과 컴퓨터링크유니트에 대한 내용을 파악해보고, 설비고장진단 및 이상발생에 대한 추적이 가능하도록 PLC Memory내에 PLC접점데이타를 직접접근방식(Direct Accssing Method)과 간접접근방식 (Indirect Accssing Method)으로 구분하고, 간접접근방식을 요소(Element), 동작(Event)에 의한 방법론을 이용하여 PLC DATA를 처리토록 하는 내용을 설명하고자 한다.

• Journal of Power Electronics
• /
• 제19권6호
• /
• pp.1380-1392
• /
• 2019

Input filters are very important to matrix converters (MCs). They are used to improve grid side current waveform quality and to reduce the input voltage distortion supplied to the grid side. Due to the effects of the input filter and the output power, the grid side power factor (PF) is not at unity when the input power factor angle is zero. In this paper, the displacement angle between the grid side phase current and the phase voltage affected by the input filter parameters and output power is analyzed. Based on this, a new grid side PF unity compensation method implemented in the indirect space vector pulse width modulation (ISVPWM) method is presented, which has a larger compensation angle than the traditional compensation method, showing a higher grid side PF at unity in a wide output power range. Simulation and experimental results verify that the analysis of the displacement angle between the grid side phase current and the phase voltage affected by the input filter and output power is right and that the proposed compensation method has a better grid side PF at unity.

• 대한인간공학회지
• /
• 제6권1호
• /
• pp.33-40
• /
• 1987

To study the body trunk basic to Clothing construction, and study the peculiarities of visual spatial division, necessary items are measured indirectly from 216 unmarried women from 19 to 24 years old by a photographic net-work method. In so doing, the problem of Fashion Design in establishing the location of Darts for Basic Dress is not considered. The following results are obtained. 1) Indirect measuring method, is obtained approximate to actual size, with an error of .+-. 2.8cm. 2) In the modeling plan of Dart location viewed from the visual-spatial division in Basic Dress, it is concluded that Darts are to be placed at the point of 1/3k+1/5k form the waist. From the aesthetic point of wiew, it is more appealling for darts to be placed at the point of 6cm .+-. 0.6cm right or left of center. 3) From direct measurement dart location can be set based on bust point width, and from indirect measurement, dart location can be set based on waist width.

• 보건교육건강증진학회지
• /
• 제10권2호
• /
• pp.32-39
• /
• 1993

The authors have examimed 349 primary school children with questionare in order to appraise the oral health educational levels, one month later after performing oral health education as direct education and indirect education with video film, and compared the data from 350 uneducated children. The obtained results were as followings ; 1. It was estimated that the direct and indirect oral health education for school children were effective for in creasing the knowledge levels of oral health. 2. It was revealed that such items of oral health education as preventive measure for caries, tooth brushing method, etiology of dental caries and etiology of malocclusion were more effective for increasing the knowledge levels, compared to uneducated group. 3. Tooth Brushing Method should be educated in practical, not only to school children but also school teachers. 4. It should be established the goals and items for oral health education in practical as national level.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 2005년도 ICCAS
• /
• pp.262-264
• /
• 2005

We propose a filter design method to suppress the effect of gyroscope mixed random errors at INS system level. It is based on the result that mixed random errors can be represented by a single equivalent ARMA model. At first step, the time difference of equivalent ARMA process is performed, which consider the characteristic of indirect feedback Kalman filter used in INS filter. Next, a state space conversion of time differenced ARMA model is achieved. If the order of AR is greater than that of MA, the controllable or observable canonical form is used. Otherwise, we introduce the state equation of which the state variable is composed of the ARMA model output and several step ahead predicts of that. At final step, a complete form state equation is presented. The simulation results shows that the proposed method gives less transient error and better convergence compared to the conventional filter which assume the mixed random errors as white noise.

• 한국지반공학회:학술대회논문집
• /
• 한국지반공학회 2010년도 춘계 학술발표회
• /
• pp.722-729
• /
• 2010

In this study, we considered the development for degree of compaction for intelligent compaction. In practice, any direct or indirect method can be used as a intelligent compaction method. A series of field tests was conducted using an accelerometer. This is quick and simple indirect methods of measuring soil stiffness. Each result was compared with the results from a plate load test. A prototype device for intelligent compaction was developed, and we evaluated its performance.

• 제어로봇시스템학회논문지
• /
• 제8권6호
• /
• pp.524-532
• /
• 2002

This paper presents a new in-flight alignment method for an SDINS under large initial heading error. To handle large heading error, a new attitude error model is introduced. The attitude errors are divided into heading error and leveling errors using a newly defined horizontal frame. Some navigation error dynamic models are derived from the attitude error model for indirect feedback filtering of the in-flight alignment system. A Kalman filter with Position measurement is designed to estimate navigation errors as the indirect feedback filter Simulation results show that the proposed in-flight alignment method reduces the heading error very quickly from more than 40deg to about 5deg so as to apply a refined navigation filter. The total alignment process including leveling mode and navigation mode in addition to the proposed one allows large initial values not only in heading error but also in leveling errors.