• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.836-840
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• 2011

In order to isolate thermophilic compost-promoting bacteria with high activity of cellulase and xylanase, spent mushroom substrates with sawdust were collected from mushroom cultivation farm, Jinju, Gyeongnam in Korea. Among of the isolates, one strain, designated SJ21 was selected by agar diffusion method. The strain SJ21 was identified as members of the Bacillus lincheniformis by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain SJ21 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rDNA gene sequence similarity of 99%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain SJ21 was classified within the genus Bacillus, for which the name Bacillus sp. SJ21 is proposed. The cellulase and xylanase activity of Bacillus sp. SJ21 was slightly increased according to bacterial population from exponential phase to stationary phase in growth curve for Bacillus sp. SJ21.

• Mass Spectrometry Letters
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• v.14 no.4
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• pp.178-185
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• 2023

QuEChERS is used worldwide as a universal sample preparation method with many benefits, such as being quick, easy, cheap, effective, rugged and safe. This study examined whether QuEChERS can be employed in isotope dilution mass spectrometry (ID-MS) for accurate analysis of pesticides in food. The ratios of fortified values and measured values of malathion and fenitrothion using the QuEChERS method were compared with those using the solid phase extract (SPE) method which was previously used in this laboratory. The separations of the two pesticides on DB-5MS and VF-1701MS columns were compared. Malathion and fenitrothion were fortified into kimchi cabbage and pretreated with the QuEChERS method and the SPE method. The results obtained using the DB-5MS column varied according to the sample preparation method, column and pesticide level. Using the VF-1701 column, ratios were 98-102% by both QuEChERS and Carb/NH₂ SPE method for all fortification level. Malathion and fenitrothion were fortified into strawberry samples for comparison with kimchi cabbage. The results for the strawberry samples indicated that the ratios were not influenced by the sample preparation methods or GC column. The QuEChERS method could be acceptable in the ID-MS method for pesticide residue analysis in food, however other conditions should be carefully considered for accurate determination, such as the column, amount of analyte and food matrix.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.55 no.4
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• pp.202-206
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• 2006

In this paper, an auto detection method of stator winding fault of small induction motor is suggested. The Park's vector pattern which is obtained from 3-phase current signal by d-q transforming, is very good to detect winding fault. Comparing the Park's vector pattern of testing motor with its of healthy motor, the Park's vector pattern of fault motor is became an ellipse and the asymmetry is increased by the winding fault series. So for detecting the dis-symmetry, id-filtered function, Min-value, and Max-value are suggested for auto detecting. Using LabVIEW programing, 3-phase healthy motor and several kind of winding fault motors are tested and the test results are shown that the suggested method can gives us a possibility of an auto detecting winding fault.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.10
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• pp.5100-5119
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• 2018

Most of existing privacy-preserving multi-authorities attribute-based encryption schemes (PP-MA-ABE) only considers the privacy of the user identity (ID). However, in many occasions information leakage is caused by the disclosing of his/her some sensitive attributes. In this paper, we propose a collusion-resisting ciphertext-policy PP-MA-ABE (CRPP-MACP-ABE) scheme with hiding both user's ID and attributes in the cloud storage system. We present a method to depict anonymous users and introduce a managerial role denoted by IDM for the management of user's anonymous identity certificate ($AID_{Cred}$). The scheme uses $AID_{Cred}$ to realize privacy-preserving of the user, namely, by verifying which attribute authorities (AAs) obtain the blinded public attribute keys, pseudonyms involved in the $AID_{Cred}$ and then distributes corresponding private keys for the user. We use different pseudonyms of the user to resist the collusion attack launched by viciousAAs. In addition, we utilize IDM to cooperate with multiple authorities in producing consistent private key for the user to avoid the collusion attack launched by vicious users. The proposed CRPP-MACP-ABE scheme is proved secure. Some computation and communication costs in our scheme are finished in preparation phase (i.e. user registration). Compared with the existing schemes, our scheme is more efficient.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2001.11a
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• pp.28-35
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• 2001

The heat transfer and pressure drop characteristics of R718 flowing in smooth horizontal copper tubes with inner diameter of 3.36 mm, 5.35 mm, 6.54 mm and 8.12 mm were investigated. The test section is a counterflow heat exchanger with refrigerant flowing in the inner tube and water flowing in the annulus. Experiments were peformed for the flowing range of variables : Reynolds number (1000 to 20000), mass flow rate of brine (450 kg/h) and refrigerant temperature (5$0^{\circ}C$). The main results were summarized as follows : (1) The heat transfer coefficient of 3.36 mm ID was about 10% to 30% higher than that of 5.35 mm, 6.54 mm and 8.12 mm ID, and the heat transfer coefficients for small diameter. tubes are about 20% to 27% higher than these predicted by Gnielinski. The new correlation is proposed to predict the experimental data. (2) As a result of comparison with correlation prosed by Blasius. the deviation of the experimental data slightly increased as the tube diameter decreased. (3) The ratio of heat transfer to friction factor (j/f) correlated by all experimental data increased as the tube diameter decreased.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.8
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• pp.1430-1435
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• 2007

We developed a 440MHz surface acoustic wave (SAW) microsensor integrated with pressure-temperature sensors and ID tag. Two piezoelectric substrates were bonded, in which ${\sim}150\;{\mu}m$ cavity was structured. Four sides were completely sealed by JSR photoresist (PR). Pressure sensor was placed on the top substrate, whereas ill tag and temperature sensor were placed on the bottom substrate. Using network analyzer, the developed microsensor was wirelessly tested. Sharp reflection peaks with high S/N ratio, small signal attenuation, and small spurious peaks were observed. All the reflection peaks were well matched with the coupling of mode (COM) simulation results. With a 10mW RF power from the network analyzer, a ${\sim}1$ meter readout distance was observed. Eight sharp ON reflection peaks were observed for ID tag. Temperature sensor was characterized from $20^{\circ}C$ to $200^{\circ}C$. A large phase shift per unit temperature change was observed. The evaluated sensitivity was ${\sim}10^{\circ}/^{\circ}C$.

• Analytical Science and Technology
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• v.23 no.6
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• pp.524-530
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• 2010

Chloramphenicol is one of the most effective antibiotics for treatment of food-producing animals for typhoid fever. However, it has been reported that it caused severe side effects such as aplastic anemia in human, therefore the use of chloramphenicol for food-producing animal is prohibited by European Union and other countries. In this study, the analytical method using isotope dilution liquid chromatography-mass spectrometry (ID-LC/MS) was established for accurate determination of chloramphenicol in meat. Chloramphenicol was extracted with ethylacetate from porcine and solid phase extraction cartridge was used for enhancing the recovery. The residue of chloramphenicol in porcine was analyzed using the liquid chromatography mass spectrometer (LC/MS) interfaced with electrospray ionization source. Analysis was performed in negative mode with selected reaction mornitoring mode at m/z 321${\rightarrow}$257 of $[M-H]^-$ ${\rightarrow}$ $[M-H-(HCOCl)]^-$ and m/z 326 ${\rightarrow}$ 262 channels for its isotope. The established method was tested using fortified samples at the level of 0.2 1, 10, $25\;{\mu}g$/kg and analytical results agreed with the gravimetrically fortified values within their uncertainties. This method was validated by analyzing a certified reference material, BCR 445, from IRMM (Institute for Reference Materials and Measurement). Our measurement values agreed with the certified value within their uncertainties. The uncertainty of our measured value was much lower than that the certified value from IRMM.

• Journal of Pharmaceutical Investigation
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• v.22 no.4
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• pp.317-321
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• 1992

A high-performance liquid chromatographic (HPLC) method was developed for the determination of diltiazem (DTZ) and its major metabolite, deacetyldiltiazem (DAD), in rat plasma. DTZ, DAD and imipramine, the internal standard, were selectively fractionated from plasma on a $C_{18}$ reversedphase column $({\mu}-Bondapak,\;10\;{\mu}m\;silica,\;300{\times}3.9\;mm\;ID)$. The composition of the mobile phase was methanol: acetonitrile: 0.04 M ammonium bromide: triethylamine (40:24:36:0.06 in volume). The pH of the mobile phase of their method was lowered to 6.4. The eluents from the column were detected for DTZ and DAD using a UV detector at 237 nm. The recovery was >85% for DTZ and DAD, and average intra-day and inter-day coefficients of variation were <6% for DTZ and DAD at the concentration ranges of 20-1000 ng/ml. Detection limit of DTZ and DAD in plasma was 20 ng/ml with signal-to-noise ratio of 3. This method would be applicable to practical pharmacokinetic studies without detriment to the HPLC column.

• Journal of the Optical Society of Korea
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• v.20 no.6
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• pp.722-732
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• 2016

A new optical one-time password (OTP) authentication method using digital holography is proposed, which enhances security strength in the authentication system. A challenge-response optical OTP algorithm based on two-factor authentication is presented using two-step phase-shifting digital holography, and two-way authentication is also performed using challenge-response handshake in both directions. Identification (ID), password (PW), and OTP are encrypted with a shared key by applying phase-shifting digital holography, and these encrypted pieces of information are verified by each party by means of the shared key. The encrypted digital holograms are obtained by Fourier-transform holography and are recorded on a CCD with 256 quantized gray-level intensities. Because the intensity pattern of such an encrypted digital hologram is distributed randomly, it guards against a replay attack and results in higher security level. The proposed method has advantages, in that it does not require a time-synchronized OTP, and can be applied to various authentication applications. Computer experiments show that the proposed method is feasible for high-security OTP authentication.

• Journal of Mushroom
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• v.15 no.4
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• pp.249-253
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• 2017

Twelve strains of bacteria with cellulase and xylanase activities were isolated from spent mushroom substrates collected from button mushroom cultivation farm, Buye, Chungcheongnam-do in Korea. Among them, one strain, designated NO12, with higher cellulase and xylanase activities was selected by agar diffusion method. The strain NO12 was identified to be a Bacillus sp. by biochemical characteristics using Bacillus ID kit and MicroLog system. Comparative 16S rDNA gene sequence analysis showed that strain NO12 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rDNA gene sequence similarity of 99.2%. Based on its physiological properties, biochemical characteristics, and phylogenetic distinctiveness, strain NO12 was classified within the genus Bacillus, for which the name Bacillus subtilis NO12 was proposed. The cellulase and xylanase activities of B. subtilis NO12 were slightly increased according to bacterial population from exponential phase to stationary phase in the growth curve for B. subtilis NO12. The xylanase activity continuously increased from the beginning of the exponential phase and exhibited maximum activity in the middle of the exponential phase.