• Journal of Microbiology and Biotechnology
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• v.24 no.8
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• pp.1065-1072
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• 2014

Doxorubicin, produced by Streptomyces peucetius ATCC 27952, is tightly regulated by dnrO, dnrN, and dnrI regulators. Genome mining of S. peucetius revealed the presence of the IclR (doxR) type family of transcription regulator mediating the signal-dependent expression of operons at the nonribosomal peptide synthetase gene cluster. Overexpression of doxR in native strain strongly repressed the drug production. Furthermore, it also had a negative effect on the regulatory system of doxorubicin, wherein the transcript of dnrI was reduced to the maximum level in comparision with the other two. Interestingly, the overexpression of the same gene also had strong inhibitory effects on the production of actinorhodin (blue pigment) and undecylprodigiosin (red pigment) in Streptomyces coelicolor M145, herboxidiene production in Streptomyces chromofuscus ATCC 49982, and spinosyn production in Saccharopolyspora spinosa NRRL 18395, respectively. Moreover, DoxR exhibited pleiotropic effects on the production of blue and red pigments in S. coelicolor when grown in different agar media, wherein the production of blue pigment was inhibited in R2YE medium and the red pigment was inhibited in YEME medium. However, the production of both blue and red pigments from S. coelicolor harboring doxR was halted in ISP2 medium, whereas S. coelicolor produced both pigmented antibiotics in the same plate. These consequences demonstrate that the on and off production of these antibiotics was not due to salt stress or media compositions, but was selectively controlled in actinomycetes.

• Journal of Mushroom
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• v.13 no.1
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• pp.56-62
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• 2015

A lipase producing bacterium was isolated from button mushroom bed, which showing high clear zone on agar media containing Tributyrin as the substrate. The strain was identified as Burkholderia cepacia by analysis of 16S rDNA gene sequence. Crude lipase (CL) was partially purified from 70% ammonium sulfate precipitation using the culture filtrate of B. cepacia. Immobilized lipases were prepared by cross-linking method with CL from B. cepacia and Novozyme lipase (NL) onto silanized Silica-gel as support. Residual activitiy of the immobilized CL (ICL) and immobilized NL (INL) was maintained upto 61% and 72%, respectively. Biodiesel (Fatty acid methyl ester, FAME) was recovered by transesterification and methanolysis of Canola oil using NaOH, CL and ICL as the catalysts to compare the composition of fatty acids and the yield of FAME. Total FAME content was NaOH $781mg\;L^{-1}$, CL $681mg\;L^{-1}$ and ICL $596mg\;L^{-1}$, in which the highest levels of FAME was observed to 50% oleic acid (C18:1) and 22% stearic acid (C18:0). In addition, the unsaturated FAME (C18:1, C18:2) decreased, while saturated FAME (C16:0, C18:0) increased according to increasing the reaction times with both CL and ICL, supporting CL possess both transesterification and interesterification activity. When reusability of ICL and INL was estimated by using the continuous reaction of 4 cycles, the activity of ICL and INL was respectively maintained 66% and 79% until the fourth reaction.

• Proceedings of the Materials Research Society of Korea Conference
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• 1999.11a
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• pp.69-69
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• 1999

• Journal of the Korean Society for information Management
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• v.5 no.1
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• pp.31-52
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• 1988

The information policy in Japan have bccir growing rapldlr 111 recent vttars. \'arioi~s level of govc~riimerital organrzatlons, ir~bt~tution arid agrircics have undt~rtak~~rr iriforrnatiori polrcy 111 order to i r l - crease information flow toward tlie society in Japan. At tlie same trme, computerized library systems also h a w uridergoirc rapid expansion. NACSIS(National Ceriter for- Science Informat~on System) wl~icl~ was cstablisl~ed bv Mirristr> of Etlr~c~arion, Sc~ence arid Culture haw witnessed dramatic adv;rnces i r i promoting for corrstructiori of database so as to provide their 11rf'3r.mat1orr nu nationwide scale. T l r i h articlr inrroduccs tlie actr\rtle> and ~mplcmeritat~o~~ of it format in^^ pol~cy wl~i(~li taktas a place hy goverrirnrlit of Japar~ aid arraly~c,.; thi~ ~ I . O I . P S I of deciiioii maltiiig iri polit~cal procedures. So far as b:ast-A\~ari riatioris art, ~ . n r i v t ~ r ~ ~ , e.;pc,ciall\. Kr,rc.an a~rd Japair !rave ,imilar cultural and liistorical backjir. ourii1, ard could t)t. tliif most a p p r o p r i a t ~ ~ caac stlldh for political ~nq~lerncritat~orr. Thus the topic of this artrclc will be useful to r t ~ a l l ~ t . Ore, trail ur~d c r r o r prorex, iri iiiior.matior~ pnlic\ulcorner.

• The Plant Pathology Journal
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• v.29 no.3
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• pp.249-259
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• 2013

Burkholderia glumae causes rice grain rot and sheath rot by producing toxoflavin, the expression of which is regulated by quorum sensing (QS). The QS systems of B. glumae rely on N-octanoyl homoserine lactone, synthesized by TofI and its cognate receptor TofR, to activate the genes for toxoflavin biosynthesis and an IclR-type transcriptional regulator gene, qsmR. To understand genome-wide transcriptional profiling of QS signaling, we employed RNAseq of the wild-type B. glumae BGR1 with QS-defective mutant, BGS2 (BGR1 tofI::${\Omega}$) and QS-dependent transcriptional regulator mutant, BGS9 (BGR1 qsmR::${\Omega}$). A comparison of gene expression profiling among the wild-type BGR1 and the two mutants before and after QS onset as well as gene ontology (GO) enrichment analysis from differential expressed genes (DEGs) revealed that genes involved in motility were highly enriched in TofI-dependent DEGs, whereas genes for transport and DNA polymerase were highly enriched in QsmR-dependent DEGs. Further, a combination of pathways with these DEGs and phenotype analysis of mutants pointed to a couple of metabolic processes, which are dependent on QS in B. glumae, that were directly or indirectly related with bacterial motility. The consistency of observed bacterial phenotypes with GOs or metabolic pathways in QS-regulated genes implied that integration RNAseq with GO enrichment or pathways would be useful to study bacterial physiology and phenotypes.

• Journal of Electrical Engineering and Technology
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• v.9 no.6
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• pp.2114-2117
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• 2014

Joint pain is generally a common disorder not only for the old aged people but also for the immunocompromised patients. The present proposed study reveals the presence of inflammatory diseases in joint generally diagnosed by removing synovial fluid and changes in the volume and composition are examined for the presence of WBC and crystals. This study implement a non-invasive approach to identify the changes in joint fluid by measuring the changes in electrical property of the synovial tissue under the influence of electrical current signal with frequency range between 100 kHz to 300 kHz. The response of tissue for the current signal was measured in terms of potential drop across the tissue. The hardware system design consists of input and output sections. The input section which applies current signal to upper limb joint region is made of ICL8038 function generator IC with amplifier and voltage to current converter. The output section picks voltage variation using metal surface electrode, amplifier, ADC, PIC microcontroller and LCD interface. 100 patient inclusive of normal and disease affected patients where examined for upper limb synovial fluid variation and inflammatory diseases were identified.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.41.3-42
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• 2021

Coma cluster (Abell 1656) is one of the most massive local galaxy clusters such as Virgo, Fornax, and Perseus, which holds a large collection of globular clusters. Globular cluster systems (GCSs) in a galaxy cluster tell us a history of hierarchical cluster assembly and intracluster GCs (ICGCs) are known to trace the gravitational potential of the galaxy cluster. Previous studies of GCSs in Coma mainly utilized data obtained using Hubble Space Telescope (HST) with high spatial resolution. However, most of the data were based on narrow-field pointing observations. In this study we present the widest survey of GCSs in the Coma cluster using the archival Subaru/Hyper Suprime-Cam (HSC) g and r images, supplemented with the archival HST images. The Coma GCSs are largely extended in E-W and SW direction, along the general direction of Coma-Abell 1367 filament. This global structure of the GCSs is consistent with the spatial distribution of the intracluster light (ICL). ICGC spatial distribution is largely extended to almost ~50% of the virial radius. Most of these ICGCs are blue and metal-poor, which supports the scenario that ICGCs are mainly originated from dwarf galaxies and some proportion from brighter galaxies. Implications of the results will be discussed.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.121-121
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• 2011

Thanks to KMTNet's wide field of view, it is time to implement imaging survey of extensive area of clusters of galaxies in the southern sky with modern instrument. As part of potential long-term survey of nearby (D < 50 Mpc) well-known clusters of galaxies, we propose a wide-field and deep survey of Fornax cluster as a first step of the project. By imaging the 400 square deg region (100 fields) enclosed within the five times virial radius of the Fornax cluster, in three SDSSfilters(g', r', i'), we can provide an unprecedented view of structure of Fornax cluster using sample from giant to dwarf galaxies. We will secure galaxies with brightness comparable to the limiting magnitude (r'=23.1 AB mag) of SDSS. Furthermore, we also request extremely deep (limiting surface brightness of ~ 28 mag $arcsec^{-2}$forr'band) survey for the central region (16 square degree, i.e., four fields) of Fornax cluster. This will allow us to detect the diffuse intracluster light (ICL) that permeates clusters as a valuable tool for studying the hierarchical nature of cluster assembly. In order to complete whole survey, about 285 hr observing time (without overhead) is required. By combining data available at other wavelengths, it will offer unique constraints on the formation of large-scale structure and also provide important clues for theories of galaxy formation and evolution. Our proposed survey will be implemented in the close collaboration with researchers in various countries (Germany, Australia, UK, USA) and ongoing project (e.g., SkyMapper).

• Fashion & Textile Research Journal
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• v.23 no.2
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• pp.261-272
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• 2021

The purpose of the present study was to evaluate the thermal insulation of air-filled winter jackets according to the amount of air-filler using a thermal manikin. The insulation of these jackets' was compared to a down padded jacket with an identical design and size. The amounts of air-filler were 100% (26,219 cm³), 70% (18,645 cm³), 50% (13,110 cm³), and 0% (0 cm³). The results showed that a clothing insulation (Icl) of 0%, 50%, 70%, and 100% air, and 100% down jackets was 0.208, 0.243, 0.207, 0.176, and 0.315 clo, respectively. In addition, the down jacket with waisttaped had a clothing insulation of 0.369 clo. However, the highest value of clothing insulation per clothing weight was the 50% air-filled jacket in all conditions. In terms of regional power consumption of the thermal manikin, the down jacket consumed less power for the shoulder and chest than the air-filled jackets. In conclusion, in order to maximize the thermal insulation of air-filled jackets, an optimal amount of air-filler, that is, an amount which does not compromise (break) the layer of inner air between the surface of manikin and the lining of the jacket, should be explored. Further studies on lining materials, end-closed design, and changes in thermal insulation under the conditions of strong wind or heavy snow are recommended.

• Bulletin of the Korean Chemical Society
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• v.35 no.5
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• pp.1516-1522
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• 2014

A series of 20 wt % $(NH_4)_2SO_4$ and 3 wt % $Al_2O_3$ surface treatments were applied to $Li[Li_{0.2}Mn_{0.54}Co_{0.13}Ni_{0.13}]O_2$ substrates. The $Li[Li_{0.2}Mn_{0.54}Co_{0.13}Ni_{0.13}]O_2$ substrates were synthesized using a co-precipitation method. Sample (a) was left pristine and variations of the 20 wt % $(NH_4)_2SO_4$ and 3 wt % $Al_2O_3$ were applied to samples (b), (c) and (d). XRD was used to verify the space group of the samples as R$\bar{3}$m. Additional morphology and particle size data were obtained using SEM imagery. The $Al_2O_3$ coating layers of sample (b) and (d) were confirmed by TEM images and EDS mapping of the SEM images. 2032-type coin cells were fabricated in a glove box in order to investigate their electrochemical properties. The cells were charged and discharged at room temperature ($25^{\circ}C$) between 2.0V and 4.8V during the first cycle. The cells were then charged and discharged between 2.0V and 4.6V in subsequent cycles. Sample (d) exhibited lower irreversible capacity loss (ICL) in the first charge-discharge cycle as compared to sample (c). Sample (d) also had a higher discharge capacity of ~250 mAh/g during the first and second charge-discharge cycles when compared with sample (c). The rate capability of the $Al_2O_3$-coated sample (b) and (d) was lower when compared with sample (a) and (c). Sample (d), coated with $Al_2O_3$ after the surface treatment with $(NH_4)_2SO_4$, showed an improvement in cycle performance as well as an enhancement of discharge capacity. The thermal stability of sample (d) was higher than that of the sample (c) as the result of DSC.