• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.137-142
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• 2015

Antifreeze proteins and glycoproteins [AF(G)Ps] constitute a group of proteins that lower the freezing but not the melting points of aqueous solutions, enabling polar and north-temperate fish to survive in ice-laden environments. However, little is known about antifreeze activity in temperate fish; such work would extend our knowledge on the functions and evolution of AF(G)Ps. In the present study, we screened for antifreeze activity in temperate fish caught off the coast of Jumunjin ($37.89^{\circ}N$), Gangneung, Korea. Thermal hysteresis (TH) and the ability to inhibit ice recrystallization (IR) in blood, liver, and muscle samples from nine fish were examined to assess antifreeze activity. As the East Sea off the coast of Jumunjin is ice-free year round, we thought it most unlikely that the fish would express antifreeze proteins. Surprisingly, the blood of Pleurogrammus azonus and three types of tissue from Gymnocanthus herzensteini, Zoarces gilli and Kareius bicoloratus exhibited measurable TH values together with the ability to trigger characteristic morphological changes in ice crystals. Blood samples from the three species also evidenced ice recrystallization (IR) inhibition. This implies that AF(G)Ps or other antifreeze-like substances are present in temperate fish even under nonfreezing conditions. These results contribute to our understanding of the functions and origins of antifreeze activity in fish.

• Journal of Industrial Technology
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• v.26 no.B
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• pp.63-71
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• 2006

The concerned object of the construction industry is environment. It is one of the main purposes of today's construction that human can live reasonably in nature. This study is about the fish-road connecting hydraulic structure in the river with nature. Chapter 2, starting with the general investigation about fish-road and helps comprehension for relatively general fish-road such as necessity, establishment purpose, history, study example, relating domestic law, design condition, consideration and form of fish-road. Chapter 3, containing the present condition of fish-road facilities in Gangwon-Do and thoughts of the problems and general countermeasures of existing fish-road. Chapter 4, Having Examined compatibility by amount of flowing water through hydraulic model test in the Ice harbor I-type model. chapter 5, Analyzation and arrangement to efficient establishment plan of fish-road and the result of the hydraulic model experiment to be beaconed a little in forward fish-road study. In the hydraulic model test result, when we suppose establishment of 1~3 in slope of 1/20, could know that applicable drought flow of the river is $0.06{\sim}3.0m^3/sec$ in case of Ice harbor I-type which transforming Ice harbor that was invented as studying project of the Ministry of Environment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.2 no.1
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• pp.49-54
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• 1973

In this paper, the effect of Ever-Fresh, a kind of preservatives for fish and shellfish, on fish and shrimp during ice storage were investigated. The results are as follows: 1. K value representing the ratio of optical densities of inosine and hypoxanthine fraction and total nucleotide fraction at 250nm could be useful as an index to measure the freshness of fish and shrimp during ice storage. 2. Volatile basic nitrogen could be also useful as an index to measure the freshness of shrimp during ice storage. 3. Ever-Fresh treatment of conger eel, half-beak, and shrimp appeared effective on keeping freshness during the early stage of ice storage. 4. Ever-Fresh treated shrimp showed lower thiobarbituric acid value as compared with the control during the early stage of ice storage.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2003.10a
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• pp.635-638
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• 2003

This study was studied in Tamjin liver that is situated jangheung-gun Jeollanm-do. Fishways that was studied with the emphasis on Ice harbour type fishway analyzed efficiencies. In result, Ice harbor type fishway discovered 12species of fish among 13species in Tamjin liver. But, most other fishways have discovered a few fishes. Because most fishways established ill station and managed badly.

• Ocean and Polar Research
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• v.25 no.4
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• pp.607-615
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• 2003

Many organisms are known to survive in icy environments. These include both over wintering terrestrial insects and plants as well the marine fish inhabiting high latitudes. The adaptation of these organisms is both a fascinating and important topic in biology. Marine teleosts in particular, can encounter ice-laden seawater that is approximately $1^{\circ}C$ colder than the colligative freezing point of their body fluids. These animals produce a unique group of proteins, the antifreeze proteins (AFPs) or antifreeze glycoproteins (AFGPs) that absorb the ice nuclei and prevent ice crystal growth. Presently, there are at least four different AFP types and one AFGP type that are isolated from a wide variety of fish. Despite their functional similarity, there is no apparent common protein homology or ice-binding motifs among these proteins, except that the surface-surface complementarity between the protein and ice are important for binding. The remarkable diversity of these proteins and their odd phylogenetic distribution would suggest that these proteins might have evolved recently in response to sea level glaciations just 1-2 million years ago in the northern hemisphere and 10-30 million years ago around Antarctica. Winter flounder, Pleuronectes americanus, has been used as a popular model to study the regulation of AFP gene expression. It has a built-in annual cycle of AFP expression controlled negatively by the growth hormone. The signal transduction pathways, transcription factors and promoter elements involved in this process have been studied in our laboratory and these studies will be presented.

• Ecology and Resilient Infrastructure
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• v.7 no.4
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• pp.320-326
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• 2020

The present study aimed to assess the longitudinal connectivity owing to migrant characteristics of the target fish. The study area was Wonju-cheon Stream, and the target species were Zacco platypus and Minnows. The HEC-RAS model was used for the computation of the flow, and the ICE (Information sur la Continuite Ecologique) method was used to analyze the longitudinal connectivity. The longitudinal connectivity was assessed using the minimum overflow height, velocity, and depth of the cross sectional structure of a plunge pool and considering the swimming speed of the target fish. Simulation results indicated that the longitudinal connectivity scores for the Zacco platypus and Minnows were approximately 76 and 23, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.1
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• pp.44-51
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• 1985

The changes of the get forming ability of common carp (Cyprinus carpio) and conger eel (Astroconger myriaster) meat during ice and frozen storage were investigated in connection with the other quality indices, such as pH, K-value, VBN, viable cell count and sensory evaluation. The shelf-life of iced common carp and conger eel as the raw materials for fish jelly product was considered to be about 16 and 13 days, respectively. Little change in gel forming ability of two species was found during frozen storage at $-30^{\circ}C$ for 3 months. From the results obtained in the examination on the gel forming abilities of the two species stored at $-30^{\circ}C$ for 3 months were superior to those stored in ice for 3 to 4 and 4 to 6 days individually. Fish jelly from the common carp was much more elastic than that of conger eel meat. The overall qualities of two species held in frozen storage as the raw material for fish jelly product were excellent.

• Ocean and Polar Research
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• v.33 no.2
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• pp.159-169
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• 2011

Antifreeze proteins (AFPs) have ice binding affinity, depress freezing temperature and inhibit ice recystallization which protect cellular membranes in polar organisms. Recent structural studies of antifreeze proteins have significantly expanded our understanding of the structure-function relationship and ice crystal growth inhibition. Although AFPs (Type I-IV AFP from fish, insect AFP and Plant AFP) have completely different fold and no sequence homology, they share a common feature of their surface area for ice binding property. The conserved ice-binding sites are relatively flat and hydrophobic. For example, Type I AFP has an amphipathic, single ${\alpha}$-helix and has regularly spaced Thr-Ala residues which make direct interaction with oxygen atoms of ice crystals. Unlike Type I AFP, Type II and III AFP are compact globular proteins that contain a flat ice-binding patch on the surface. Type II and Type III AFP show a remarkable structural similarity with the sugar binding lectin protein and C-terminal domain of sialic acid synthase, respectively. Type IV is assumed to form a four-helix bundle which has sequence similarity with apolipoprotein. The results of our modeling suggest an ice-binding induced structural change of Type IV AFP. Insect AFP has ${\beta}$-helical structure with a regular array of Thr-X-Thr motif. Threonine residues of each Thr-X-Thr motif fit well into the ice crystal lattice and provide a good surface-surface complementarity. This review focuses on the structural characteristics and details of the ice-binding mechanism of antifreeze proteins.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.5
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• pp.1841-1850
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• 2013

The various types of fishways are installed at the multi-functional weirs in the four major rivers to minimize the negative effect due to the construction of the transverse structures. The movable weir was installed at the upstream of the ice-harbor type artificial fishway of the Dalseong weir in the Nakdong river, which can control the fishway flowrate regardless of the river flowrate. The incoming flowrate to the artificial fishway is closely related with the hydraulic characteristics that dominate the fish passage efficiency. Thus, it is crucial to find out the weir operation rule for properly sustaining efficient fish-passage, such as the optimized flowrate. In this study, the FLOW-3D was used to analyze and compare the various hydraulic characteristics associated with the passage efficiency, based upon the given different flowrate, and subsequently provide the optimized flowrate for the fishway movable weir to maintain the best efficient flow condition for the fish-passage.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.20 no.3
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• pp.91-96
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• 2014

TTI was applied to monitor the quality changes of fish displayed at a mock store. Chilled fishes were displayed with TTI on a styrofoam box filled with crushed ice. The ice was periodically refilled to maintain the fish freshness. The color of TTI and the qualities of mackerel and Alaska pollack were measured during displaying. VBN and Pseudomonas spp. were used as the quality factors of mackerel and Pollack, respectively. The spoilage time was regarded as when the factors reached their critical levels. The fishes were spoiled when the color of TTI reached an end-point. It was therefore found out that it is possible to predict the fish spoilage by observing the TTI color change.