• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2002년도 추계학술대회초록집
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• pp.148-148
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• 2002

수분부족 및 hyperoxaluria가 유도된 랫드에서 enrofloxacin 투여로 인한 신장손상을 알아보기 위하여 본 실험을 실시하였다. 랫드는 모두 4 그룹으로 나누어 실험에 공하였다. 실험은 72시간 탈수를 유발시킨 랫드 (Group 1), 3％ sodium oxalate를 음수로 7 일간 급여해서 hyperoxaluria 상태를 유발시킨 랫드 (Group 2, 3) 그리고 sodium oxalate 30mg/Kg을 1회 복강내 투여로 급성 hyperoxaluria 상태를 유발시킨 랫드(Group 4)에 각각 enrofloxacin을 용량별 (0mg/Kg, 50mg/Kg, 500mg/Kg)로 1일 혹은 7일 동안 투여한 후 임상 및 병리조직학적 소견을 추구하였던 바 다음과 같은 결과를 얻었다. 수분부족이나 hyperoxaluria 가 유도된 상태의 전 그룹에서 enrofloxacin 투여용량이 증가할수록 식욕감소, 음수량 감소, 행동둔화 등의 임상증상과 사구체의 손상, 신장피질내 충혈, 세뇨관의 변성, 공포화, 괴사 등의 변화가 현저히 진행되는 것을 알 수 있었고, Group 2, 3, 4 실험군의 소변을 원심분리하여 침전된 뇨침사를 현미경으로 검사한 결과 모든 군에서 calcium oxalate crystal이 검출되었고 일부는 magnesium ammonium phosphate crystal이 검출되었다. 이상의 결과를 종합해 볼 때, 탈수상태나 혈중 oxalate함량이 높은 랫드에서 enrofloxacin의 투여는 신장에 손상을 주어 결석을 형성할 수도 있는 것으로 사료되며 이러한 개체에서 enrofloxacin의 사용상 주의가 요구된다.

• 한국임상수의학회지
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• 제20권4호
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• pp.449-457
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• 2003

To investigate the renal effects of enrofloxacin administration on rats induced with dehydration or hyperoxaluria, male rats were treated with enrofloxacin of 50 mg to 500 mg/kg b.w.. The microscopical observations of kidney and urine sediment were carried out in the experimental groups. The result obtained were as follows; The male rats deprived of water for 72 hours and administered with enrofloxacin. As enrofloxacin administration dose was increased, clinical signs such as loss of appetite, depression, weakness, and loss of urine output became more severe. In the histopathological findings, there were hyperemia and hemorrhage in renal cortex, vacuolation and necrosis of renal tubular epithelia, proteinous casts within renal tubules. The male rats were orally administered with sodium oxalate and injected with enrofloxacin for 7days. As enrofloxacin administration dose was increased, clinical signs such as the loss of appetite and water consumption, and weakness became more severe. In the histopathological findings, there are hemorrhage of glomeruli and cortical hyperemia, vacuolation and necrosis of tubular epithelia, proteinous casts in renal tubules. In the microscopical findings of urine sediment, there are calcium oxalate crystal (diamond-like type) and magnesium ammonium phosphate crystals (rhomboid). The male rats were intraperitoneally injected with sodium oxalate and administered with enrofloxacin for 7days. As enrofloxacin administration dose was increased, clinical signs such as the loss of appetite and water consumption, weakness were more severe. In the histopathological findings, there were hyperemia and hemorrhage in both glomeruli and renal cortex. Severe necrosis of renal tubular epithelia, bluish materials within renal tubules were also found. In the microscopical findings of urine sediment, there were many calcium oxalate crystals. The present results suggest that enrofloxacin has some injurious effects in rats having dehydration or hyperoxaluria, and clinically, we should consider these renal injury effects when we use enrofloxacin in patients accompanied renal disease, dehydration and hyperoxaluria conditions.

• Childhood Kidney Diseases
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• 제23권2호
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• pp.59-66
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• 2019

Background: Primary hyperoxaluria (PH), a rare inborn error of glyoxylate meta bolism causing overproduction of oxalate, is classified into three genetic subgroups: type 1-3 (PH1-PH3) caused by AGXT, GRHPR, and HOGA1 gene mutations, respectively. We performed a retrospective case series study of Korean pediatric patients with PH. Methods: In total, 11 unrelated pediatric patients were recruited and their phenotypes and genotypes were analyzed by a retrospective review of their medical records. Results: Mutational analyses revealed biallelic AGXT mutations (PH1) in nine patients and a single heterozygous GRHPR and HOGA1 mutation in one patient each. The c.33dupC was the most common AGXT mutation with an allelic frequency of 44%. The median age of onset was 3 months (range, 2 months-3 years), and eight patients with PH1 presented with end stage renal disease (ESRD). Patients with two truncating mutations showed an earlier age of onset and more frequent retinal involvement than patients with one truncating mutation. Among eight PH1 patients presenting with ESRD, five patients were treated with intensive dialysis followed by liver transplantation (n=5) with/without subsequent kidney transplantation (n=3). Conclusion: Most patients presented with severe infantile forms of PH. Patients with two truncating mutations displayed more severe phenotypes than those of patients with one truncating mutation. Sequential liver and kidney transplantation was adopted for PH1 patients presenting with ESRD. A larger nation-wide multicenter study is needed to confirm the genotype-phenotype correlations and outcomes of organ transplantation.

• 대한유전성대사질환학회지
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• 제5권1호
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• pp.27-32
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• 2005

• Advances in Traditional Medicine
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• 제9권3호
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• pp.259-267
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• 2009

Curcumin (CMN) is known to have beneficial role in anorexia, coryza, cough, diabetic wounds, and hepatic disorders apart from its inherent antioxidant effects. Therefore, the present study was aimed to evaluate antioxidant effect of CMN in prevention of nephrolithiasis in rats-induced by ethylene glycol (EG) and Vitamin D3 (Vit. D3). Male Wistar rats (175 - 200 g) were randomized in groups like control, EG + Vit. D3 induced nephrolithiatiatic rats, CMN treated rats, CMN + EG + Vit. D3 treated rats, Vit. E + EG + Vit. D3 treated rats. Urine was collected weekly throughout the experimental protocol and estimated for calcium oxalate (CaO) count. After completion of experimental protocol serum was estimated for blood urea nitrogen and creatinine. Both the kidneys were excised and used to evaluate levels of biomarkers of oxidative stress and calcium oxalate crystal deposition by histopathological studies. Administration of EG and Vit. D3 to rats resulted in increased oxidative stress, hyperoxaluria and renal deposition of CaO crystals. Supplementation with CMN improves kidney function, reduces elevated oxidative stress, urinary oxalate level and renal deposition of CaO which shows its protective action in nephrolithiasis. The increased deposition of stone in the kidney and stone forming constituents of nephrolithiatic rats were effectively lowered by treatment of CMN.

• 한국식품영양학회지
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• 제10권1호
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• pp.44-52
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• 1997

요석증은 비뇨기과에서 흔히 관찰되는 질환으로서 30∼50대에 많이 발생하고, 남자가 여자보다 발생빈도가 높고, 좌측보다는 우측이 많으며, 문화가 발달한 나라에서는 상부요로 결석이 많으나 후진국에서는 하부요로 결석이 많다. 결석성분은 수산칼슘, 인산칼슘, 인산마그네슘암모늄, 요산, 시스틴 결석과 그들의 혼합결석으로 분류된다. 병태 생리학에 따르면, 결석의 발생원인은 요중의 무기질 농도가 과포화 상태가 되면 결정체핵이 생성된다는 과포화/결정체설, 기질관여설, 억제물질의 결핍설, 에피택시설 등이 있고, 상피소체기능항진중, 과칼슘뇨증, 과요산뇨증, 과수산뇨증, 시스틴뇨증 외에도 원위세뇨관 산증, 과비타민 D증, 소화기계 질환이 통계적으로 유의성 있게 관여된다는 것이다. 본 연구에서는 미란성 위염 환자 K43에서 화학분석법, 적외선 흡수 분석법, image analysis법을 사용하여 재발성 상부요로 결석성분을 분석한 결과 제2 수산칼슘과 인산칼슘의 혼합결석으로 확인되었고, 혈액화학 검사와 요 검사 등 임상검사 성적은 정상치를 유지하고 있었다. 따라서 K43의 재발성 요로결석은 보고된 생성원인이나 인자들에 대한 통계수치와는 연관성이 없었으며 미란성 위염으로부터 영향을 받았다는 증거도 없었다.

• Natural Product Sciences
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• 제13권3호
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• pp.180-185
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• 2007

Dichrostachys cinerea (Linn.) is commonly known as Vadatalla and used as phytotherapeutic agent. Tender shoots of the plant bruised and applied to the eyes in case of ophthalmia. The root is astringent and used in rheumatism, urinary calculi and renal troubles. The effect of the Ethanolic and aqueous extract of the root of D. cinerea were studied for its anti-urolithiatic and diuretic activity at 200 mg/kg dose level in male Wistar albino rats. Ethylene glycol feeding resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. Supplementation with aqueous and ethanolic extract of the plant significantly reduced the elevated urinary oxalate, showing a regulatory action on endogenous oxalate synthesis. Compared to ethanolic extract, aqueous extract exhibited significant anti-urolithiatic activity. Both the extracts showed significant diuretic activity. The results of our present study supports folklore claim of D. cinerea.

• Journal of Preventive Medicine and Public Health
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• 제39권2호
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• pp.165-170
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• 2006

Objectives : The determination of oxalate in urine is required for the diagnosis and treatment of primary hyperoxaluria, idiopathic stone disease and various intestinal diseases. We examined the possibility of using Fourier transform near-infrared (FT-NIR) spectroscopy analysis to quantitate urinary oxalate. The practical advantages of this method include ease of the sample preparation and operation technique, the absence of sample pre-treatments, rapid determination and noninvasiveness. Methods : The range of oxalate concentration in standard urine solutions was $0-221mg/{\ell}$. These 80 different samples were scanned in the region of 780-1,300 nm with a 0.5 nm data interval by a Spectrum One NTS FT-NIR spectrometer. PCR, PLSR and MLR regression models were used to calculate and evaluate the calibration equation. Results : The PCR and PLSR calibration models were obtained from the spectral data and they are exactly same. The standard error of estimation (SEE) and the % variance were $10.34mg/{\ell}$ and 97.86%, respectively. After full cross validation of this model, the standard error of estimation was $5,287mg/{\ell}$, which was much smaller than that of the pre-validation. Furthermore, the MCC (multiple correlation coefficient) was 0.998, which was compatible with the 0.923 or 0.999 obtained from the previous enzymatic methods. Conclusions : These results showed that FT-NIR spectroscopy can be used for rapid determination of the concentration of oxalate in human urine samples.