• Reproductive and Developmental Biology
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• v.30 no.2
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• pp.81-85
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• 2006

This study was conducted to examine the effect of IRES controlled reporter gene on screening and production of recombinant human erythropoietin (EPO) proteins from cultured CHO cells. The cDNA was cloned for EPO from human liver cDNA Using site-directed mutagenesis, we generated recombinant human EPO (rhEPO) with two additional N-glycosylations (Novel erythropoiesis-stimulating protein: NESP). Wild type hEPO and NESP were cloned into expression vectors with GFP reporter gene under regulatory control of CMV promoter and IRES so that the vectors could express both rhEPO and GFP. The expression vectors were transfected to cultured CHO-K1 cells. Under microscopy, expression of GFP was visible. Using supernatant of the culture, ELISA assay, immunocytochemistry and in vitro assay using EPO dependant cell line were performed to estimate biological activity to compare the production characteristics (secretion levels, etc.) between rhEPO and NESP. The activity of NESP protein, obtained by mutagenesis, was described and compared with its rhEPO counterpart produced under same conditions. Although NESP had less secretion level in CHO cell line, the biological activity of NESP was greater than that of rhEPO. These results are consistent with previous researches. We also demonstrated that rhEPO and GFP proteins expressed simultaneously from transfected CHO cell line. Therefore we conclude that use of GFP reporter gene under IRES control could be used to screen and produce rhEPO in cultured CHO cells.

• Journal of the Korean Society of Food Culture
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• v.24 no.2
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• pp.199-205
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• 2009

The purpose of this study was to investigate the quality characteristics of cookies prepared with shrimp powder (0, 3, 5, 7, 9%) substituted for flour as a snack food for Kindergarteners. The pH of the cookie dough decreased significantly in response to the addition of all levels of shrimp powder. However, there were no significant differences in the dough values among the test groups. Furthermore, when the spread factor values were compared among groups, they were found to be inversely proportional to the shrimp powder concentration. In addition, the Hunter's color L, a and b values decreased significantly as the level of shrimp powder increased. Moreover, the addition of 3${\sim}$9% shrimp powder resulted in increased hardness, cohesiveness, springiness, and brittleness when compared to the control. Finally, the results of acceptance test showed that the cookies containing 5% shrimp powder had the highest scores.

• Electronics and Telecommunications Trends
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• v.39 no.4
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• pp.63-72
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• 2024

Vision is a process in which the brain and eyes collaborate to enable sight by analyzing light reflected from objects. Vision is also the most crucial among the five basic human senses for recognizing environments. The eyes contain 70% of the sensory receptors in the body, and 90% of the information processed by the brain is visual. Currently, approximately 2.2 billion people worldwide have vision impairments. A recent study estimated that the global economic productivity losses due to vision impairment and blindness amount to approximately $410 billion. Additionally, as people age, their ability to control their vision declines, leading to presbyopia, which typically starts in their 40s. Since people heavily rely on vision in their daily lives, vision problems can significantly reduce the quality of life. Approaches to solving vision problems can be broadly categorized into visual prostheses requiring surgery, sensory substitution based on neuroplasticity, and smart glasses for presbyopia. We present the trends and future development prospects for three key areas of research: visual prostheses, visual substitution technologies, and smart glasses technologies. These areas are being explored with the aim of addressing visual impairments and blindness.

• BMB Reports
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• v.53 no.7
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• pp.379-384
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• 2020

Exposure to Ultraviolet (UV) light induces photoaging of skin, leading to wrinkles and sunburn. The perennial herb Humulus japonicus, widely distributed in Asia, is known to have anti-inflammatory, antimicrobial, and antioxidant effects. However, the physiological activities of isolated compounds from H. japonicus have rarely been investigated. This study focused on the isolation of active compounds from H. japonicus and the evaluation of their effects on photoaging in UVB-irradiated human fibroblast (Hs68) cells. When the extract and four fractions of H. japonicus were treated respectively in UVB-irradiated Hs68 cells to investigate anti-photoaging effects, the ethyl acetate (EtOAc) fraction showed the strongest inhibitory effect on MMP1 secretion. From EtOAc fraction, we isolated luteolin-8-C-glucoside (1), apigenin-8-C-glucoside (2), and luteolin-7-O-glucoside (3). These compounds suppressed UVB-induced MMP-1 production by inhibiting the phosphorylation of the mitogen-activated protein kinases (MAPKs) and activator protein-1 (AP-1). When the antioxidant activity of the compounds were estimated by conducting western blot, calculating the bond dissociation energies of the O-H bond (BDE) at different grade, and measuring radical scavenging activity, we found luteolin-8-C-glucoside (1) showed the strongest activity on the suppression of UVB-induced photoaging. These results demonstrate the inhibitory effect of three flavone glycosides derived from H. japonicus on MMP-1 production, MAPK and AP-1 signaling, and oxidative stress; this could prove useful in suppressing UVB induced photoaging.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.97-102
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• 2005

One of the key molecules involved in skin moisture is hyaluronan (hyaluronic acid, HA) with its associated water of hydration. The predominant component of the ECM (extracellular matrix) of skin is HA. It Is the primordial and the simplest of the GAGs (glycosaminoglycans), a water-sorbed macromolecule In extracellular matrix, Included between the vital cells of epidermis. In the skin, HA was previously thought to derive extlusively from dermis. But, recent studies revealed that HA could be synthesized in epidermis. Flavonoids are polyphenolic compounds that is found mainly in foods of plant origin. Kaempferol was known to increase glutathione synthesis in human keratinocyte. And quercetin blocked PPAR-meidated keratinocyte differentiation as lipoxygenase inhibitors. In this study, we sought to evaluate the effect of flavonid, kaempferol and quercetin on production HA in keratinocyte. We examined the changes of three human hyaluronan synthase genes (HASI, HAS2, HAS3) expression by semi-quantitative RT-PCR when kaempferol or quercetin was added to cultured human keratinocytes. We found that these flavonoids slightly upregulated HAS2, HAS3 mRNA after 24 h. And we investigated the effect on HA production by ELISA. When we evaluated the level of HA in culture medium after 24 h incubation. We found enhanced accumulation of HA in the culture medium. Although the effects of above flavonoids are less than retinoic acid, the data indicate that kaempferol, quercetin can dose-dependently increase the level of HA in epidermis cell line. It suggested that flavonoid, kaempferol, and quercetin increased production of HA in skin and it helped to elevate skin moisture and improve facial wrinkle.

• Journal of the Korean Society of Industry Convergence
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• v.21 no.2
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• pp.87-93
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• 2018

This Study Propose A New Approach To Impliment A Intelligent Robot Control Based on Voice Recognition For Smart Factory Automation Since human usually communicate each other by voices, it is very convenient if voice is used to command humanoid robots or the other type robot system. A lot of researches has been performed about voice recognition systems for this purpose. Hidden Markov Model is a robust statistical methodology for efficient voice recognition in noise environments. It has being tested in a wide range of applications. A prediction approach traditionally applied for the text compression and coding, Prediction by Partial Matching which is a finite-context statistical modeling technique and can predict the next characters based on the context, has shown a great potential in developing novel solutions to several language modeling problems in speech recognition. It was illustrated the reliability of voice recognition by experiments for humanoid robot with 26 joints as the purpose of application to the manufacturing process.

• Korean Journal of Environmental Agriculture
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• v.28 no.3
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• pp.289-294
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• 2009

Concerning about the negative impact of chemical pesticides on human health and the environment has been leading to dramatic increase of research in natural product-based pesticides. An antagonistic bacterium Streptomyces sp. S-1110 was isolated from apple farm soil. The culture filtrate of the strain showed growth inhibition effects to apple pathogenic fungi, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Rhizoctonia solani. The unidentified antibiotic substances from the strain kept antagonistic activity either after heat treatment at $121^{\circ}C$ for 1 h or pH treatment at range of pH 3 - pH 12 for 24 h. The substances also prevented apple fruit from spoiling by inoculated two pathogenic molds, B. dothidea and C. gloeosporioides. These results suggested that the isolated strain would be useful as a biocontrol agent to control apple spoiling occurred from mold.

• International Journal of Human Ecology
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• v.10 no.1
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• pp.35-45
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• 2009

Six healthy female volunteers twice undertook an experiment with different types of clothing leaving the arms and legs covered or uncovered at $24{\pm}0.5^{\circ}C$ and $50{\pm}5%$ RH to study how different thermal stimulation to the distal extremities during the afternoon could modulate circadian parameters of body temperature rhythm. One type of clothing consisted of long-sleeved shirts and full-length trousers (Type I, 989 g, 0.991 clo); the other type consisted of half-sleeved shirts and knee-length trousers (Type II, 750 g, 0.747 clo). Subjects wore Type I or Type II clothing during the afternoon (14:00 h - 19:00 h), and Type I clothing during the evening (19:00 h - 22:30 h) and the night sleep (22:30 h - 06:00 h). Rectal temperature and skin temperatures at the arm and leg were measured continuously. Results were as follows: 1) The circadian amplitude of rectal temperature tended to be greater, and the acrophase was significantly earlier when wearing Type II rather than Type I clothing. 2) The circadian nadirs of skin temperatures of the arm and leg were significantly lower and the amplitudes were significantly greater with Type II clothing. In addition, the acrophase and bathyphase of the circadian rhythm of arm skin temperature were significantly earlier with Type II than Type I clothing. 3) The amplitude of rectal temperature was related closely with that of arm and leg skin temperature. These results suggest that a slightly cool thermal stress during the afternoon to the arms and legs exerted by wearing half-sleeved shirts and knee-length trousers induces a greater amplitude and a phase advance of the overt circadian rhythm of body temperature.

• Archives of Pharmacal Research
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• v.23 no.6
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• pp.605-612
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• 2000

Green tea polyphenols (GTP) have been demonstrated to suppress tumorigenesis in several chemical-induced animal carcinogenesis models, and predicted as promising chemopreventive agents in human. Recent studies of GTP extracts showed the involvement of mitogen-activated protein kinases (MAPKs) in the regulation of Phase II enzymes gene expression and induction of apoptosis. In the current work we compared the biological actions of five green tea catechins: (1) induction of ARE reporter gene, (2) activation of MAP kinases, (3) cytotoxicity in human hepatoma HepG2-C8 cells, and (4) caspase activation in human cervical squamous carcinoma HeLa cells. For the induction of phase IIgene assay, (-)-epigallocatechin-3-gallate (EGCG) and (-)-epicatechin-3-gallate (ECG) potently induced antioxidant response element (ARE)-mediated luciferase activity, with induction observed at 25 $\mu\textrm{m}$with EGCG. The induction of ARE reporter gene appears to be structurally related to the 3-gallate group. Comparing the activation of MAPK by the five polyphenols, only EGCG showed potent activation of all three MAPKs (ERK, JNK and p38) in a dose- and time-dependent manner, whereas EGC activated ERK and p38. In the concentration range of 25 $\mu\textrm{m}$ to 1 mM, EGCG and ECG strongly suppressed HepG2-ARE-C8 cell-growth. To elucidate the mechanisms of green tea polyphenol-induced apoptosis, we measured the activation of an important cell death protein, caspase-3 induced by EGCG, and found that caspase-3 was activated in a dose- and time-dependent manner. Interestingly, the activation of caspase-3 was a relatively late event (peaked at 16 h), whereas activation of MAPKs was much earlier (peaked at 2 h). It is possible, that at low concentrations of EGCG, activation of MAPK leads to ARE-mediated gene expression including phase II detoxifying enzymes. Whereas at higher concentrations of EGCG, sustained activation of MAPKs such as JNK leads to apoptosis. These mechanisms are currently under investigation in our laboratory. As the most abundant catechin in GTP extract, we found that EGCG potently induced ARE-mediated gene expression, activated MAP kinase pathway, stimulated caspase-3 activity, and induced apoptosis. These mechanisms together with others, may contribute to the overall chemopreventive function of EGCG itself as well as the GTP.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.6 no.2
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• pp.9-14
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• 2012

In this study, an advanced knee joint for active prosthesis leg driven by a linear actuator is suggested. The structure of knee joints of existing active prosthesis legs consists of three links. This kind of linkage requires large torque to drive the active prosthesis legs. Thus a new linkage structure is suggested to solve such problem in this paper. Motion characteristics of the suggested linkage are examined in the simulation. The motion simulation results show that the proposed linkage is able to imitate human gait cycles with the half of linear actuator speed in existing linkages.