Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Isolation and Production of Antibiotic Substance from Streptomyces sp. S-1110 Antagonistic to Multiple Apple Mold Diseases

사과 곰팡이병에 길항하는 Streptomyces sp. S-1110의 분리 및 길항 물질의 생산

  • Shin, Jin-Ho (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Eun-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Sun-Ji (School of Applied Biosciences, Kyungpook National University) ;
  • Rhee, In-Koo (School of Applied Biosciences, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University)
  • 신진호 (경북대학교 응용생명과학부) ;
  • 김은정 (경북대학교 응용생명과학부) ;
  • 박선지 (경북대학교 응용생명과학부) ;
  • 이인구 (경북대학교 응용생명과학부) ;
  • 신재호 (경북대학교 응용생명과학부)
  • Published : 2009.09.30
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Abstract

Concerning about the negative impact of chemical pesticides on human health and the environment has been leading to dramatic increase of research in natural product-based pesticides. An antagonistic bacterium Streptomyces sp. S-1110 was isolated from apple farm soil. The culture filtrate of the strain showed growth inhibition effects to apple pathogenic fungi, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Rhizoctonia solani. The unidentified antibiotic substances from the strain kept antagonistic activity either after heat treatment at $121^{\circ}C$ for 1 h or pH treatment at range of pH 3 - pH 12 for 24 h. The substances also prevented apple fruit from spoiling by inoculated two pathogenic molds, B. dothidea and C. gloeosporioides. These results suggested that the isolated strain would be useful as a biocontrol agent to control apple spoiling occurred from mold.

사과 재배에 피해를 주고 있는 사과 겹무늬썩음병, 탄저병, 라이족토니아병을 복합적으로 방제하기 위한 기초연구로서 각 병원균에 대하여 우수한 길항력을 동시에 가지는 길항미생물을 선발하고 Streptomyces sp. S-1110으로 동정하였다. 분리된 길항미생물은 배양상등액으로 길항물질을 분비하였으며 우수한 열안정성과 pH 안정성을 가지는 특성이 있었으며 각 병원균에 대한 감수성이 조금씩 달라서 2 종 이상의 길항물질이 동시에 생산되어 길항작용을 하는 것으로 추정되었다. 또한 분리균이 생산하는 길항물질을 사과 과실에 직접접종된 사과 겹무늬썩음병과 탄저병의 방제를 위해 실험한 결과 높은 방제력을 보였다. 따라서 분리된 길항미생물은 사과 과수원 및 저장소에서 동시에 수종의 질병방제에 쓰일 수 있는 천연 복합방제제의 개발을 위한 기초 균주로의 가치가 높은 것으로 판단된다.

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