• Journal of Family Resource Management and Policy Review
• /
• v.27 no.3
• /
• pp.67-75
• /
• 2023

This study will examine the changing nature of housework by analyzing participation in domestic work among 30-somethings according to generational and life cycle characteristics. To this end, 2,687 men and women in their 30s were taken from the 2020 Family Status Survey data, and a latent class analysis was conducted to categorize their participation in housework. The subjects were categorized into three groups: overall non-participation (18.05%), overall participation (59.96%), and intensive cleaning participation (21.99%). Gender, employment status, family life cycle, and attitudes about gender roles were significantly related to participation in housework. Men were more likely to be in the overall non-participation group, while women were more likely to be in the overall participation group. Individuals in the pre-formative period of the family life cycle were more likely to be in the overall non-participation group, while those in the formative and expanding periods were more likely to be in the overall participation group. The results of this study suggest that gender inequality in housework is common in the younger generation; the results also show that, in the same generation, individual participation in housework differs according to family life cycle.

• Journal of Family Resource Management and Policy Review
• /
• v.28 no.1
• /
• pp.13-25
• /
• 2024

The rate of single-person households has been increasing all over the world, and there has been a particularly rapid increase in them in Korea. Single-person households show unique and various characteristics related to the reasons for becoming a single-person household, gender, life cycle, and so on. Thus, research needs to focus on the specific groups of single-person households in order to provide tailored policies and programs. This study segmented single-person households in three groups based on life cycle: young, middle-aged, and older adults. Differences in the level of healthy lifestyle, as well as factors affecting that, were investigated according to the groups. The data were collected in 2022, with 237 respondents from single-person households in Kimpo. Descriptive statistics, t-test, ANOVA, and multiple regression analysis were conducted. The overall level of healthy lifestyle was found to be significantly higher for young adults compared to older adults. Results from multiple regression show that significant factors related to the healthy lifestyle of single-person households were gender, educational attainment, whether becoming a single-person household was voluntary, and whether the respondents had experienced discrimination as a single-person household. Significant factors differed by the sub-categories of the healthy lifestyle scale. This study has implications related to discovering differences in the level of healthy lifestyles of single-person households through examining the factors affecting it according to life cycle.

• Biomolecules & Therapeutics
• /
• v.19 no.2
• /
• pp.211-217
• /
• 2011

Panax ginseng CA Meyer, a herb from the Araliaceae, has traditionally been used as a medicinal plant in Asian countries. Ginseng extract fermented by ginsenoside-${\beta}$-glucosidase treatment is enriched in ginsenosides such as Rh2 and Rg3. Here we show that a fermented ginseng extract, BST204, has anti-proliferative and anti-invasive effects on HT-29 human colon cancer cells. Treatment of HT-29 cells with BST204 induced cell cycle arrest at $G_1$ phase without progression to apoptosis. This cell cycle arrest was accompanied by up-regulation of tumor suppressor proteins, p53 and p21$^{WAF1/Cip1}$, down-regulation of the cyclin-dependent kinase/cyclins, Cdk2, cyclin E, and cyclin D1 involved in $G_1$ or $G_1/S$ transition, and decrease in the phosphorylated form of retinoblastoma protein. In addition, BST204 suppressed the migration of HT-29 cells induced by 12-O-tetradecanoylphorbol-13-acetate, which correlated with the inhibition of metalloproteinase-9 activity and extracellular signal-regulated kinase activity. The effects of BST204 on the proliferation and the invasiveness of HT-29 cells were similar to those of Rh2. Taken together, the results suggest that fermentation of ginseng extract with ginsenoside-${\beta}$-glucosidase enhanced the anti-proliferative and the anti-invasive activity against human colon cancer cells and these anti-tumor effects of BST204 might be mediated in part by enriched Rh2.

• Current Research on Agriculture and Life Sciences
• /
• v.32 no.4
• /
• pp.199-202
• /
• 2014

The bioconversion of cellulosic biomass hydrolyzates consisting mainly of glucose and xylose requires the use of engineered Saccharomyces cerevisiae expressing a heterologous xylose pathway. However, there is concern that a fungal xylose pathway consisting of NADPH-specific xylose reductase (XR) and $NAD^+$-specific xylitol dehydrogenase (XDH) may result in a cellular redox imbalance. However, the glycerol biosynthesis and glycerol degradation pathways of S. cerevisiae, termed here as the glycerol cycle, has the potential to balance the cofactor requirements for xylose metabolism, as it produces NADPH by consuming NADH at the expense of one mole of ATP. Therefore, this study tested if the glycerol cycle could improve the xylose metabolism of engineered S. cerevisiae by cofactor balancing, as predicted by an in-silico analysis using elementary flux mode (EFM). When the GPD1 gene, the first step of the glycerol cycle, was overexpressed in the XR/XDH-expressing S. cerevisiae, the glycerol production significantly increased, while the xylitol and ethanol yields became negligible. The reduced xylitol yield suggests that enough $NAD^+$ was supplied for XDH by the glycerol cycle. However, the GPD1 overexpression completely shifted the carbon flux from ethanol to glycerol. Thus, moderate expression of GPD1 may be necessary to achieve improved ethanol production through the cofactor balancing.

• BMB Reports
• /
• v.47 no.11
• /
• pp.631-636
• /
• 2014

Aurora-A is a centrosome-localized serine/threonine kinase that is overexpressed in multiple human cancers. We previously reported an intramolecular inhibitory regulation of Aurora-A between its N-terminal regulatory domain (Nt, amino acids [aa] 1-128) and the C-terminal catalytic domain (Cd, aa 129-403). Here, we demonstrate that although both Aurora-A mutants (AurA-K250G and AurA-D294G/Y295G) lacked interactions between the Nt and Cd, they also failed to interact with Ajuba, an essential activator of Aurora-A, leading to loss of kinase activity. Additionally, overexpression of either of the mutants resulted in centrosome amplification and mitotic spindle formation defects. Both mutants were also able to cause G2/M arrest and apoptosis. These results indicate that both K250 and D294/Y295 are critical for direct interaction between Aurora-A and Ajuba and the function of the Aurora-A complex in cell cycle progression.

• Journal of Life Science
• /
• v.16 no.4
• /
• pp.589-597
• /
• 2006

Genistein, a natural isoflavonoid phytoestrogen, is a strong inhibitor of protein tyrosine kinase and DNA topoisomerase activities. There are several studies documenting molecular alterations leading to cell cycle arrest and induction of apoptosis by genistein as a chemopreventive agent in a variety of cancer cell lines; however, its mechanism of action and its molecular targets on human bladder carcinoma and leukemic cells remain unclear. In the present study, we have addressed the mechanism of action by which genistein suppressed the proliferation of T24 bladder carcinoma and U937 leukemic cells. Genistein significantly inhibited the cell growth and induced morphological changes, and induced the G2/M arrest of the cell cycle in both T24 and U937 cells with a relatively stronger cytotoxicity in U937. The G2/M arrest in T24 cells was associated with the inhibition of cyclin A, cyclin B1 and Cdc25C protein expression without alteration of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21(WAF1/CIP1). However, the inhibitory effects of genistein on the cell growth of U937 cells were connected with a marked inhibition of cyclin B1 and an induction of Cdk inhibitor p21 proteins by p53-independent manner. These data suggest that genistein may exert a strong anticancer effect and additional studies will be needed to evaluate the different mechanisms between T24 and U937 cells.

• Journal of Life Science
• /
• v.18 no.6
• /
• pp.884-890
• /
• 2008

Genistein, an isoflavone in soybean products, is a potential chemopreventive agent against various types of cancer. There are several studies documenting molecular alterations leading to cell cycle arrest at G2/M phase and induction of apoptosis; however, its mechanism of action and its molecular targets on the prostaglandin $E_2$ ($PGE_2$) production and telomere length regulation in human cancer remain unclear. In this study, we investigated the effect of genistein on the levels of cyclooxygenases (COXs) and telomere regulatory components of several human cancer cell lines (T24, human bladder carcinoma cells; U937, human leukemic cells; AGS, human stomach adenocarcinoma cells and SK-MEL-2, human skin melanoma cells). Genistein treatment resulted in the inhibition of cancer cell proliferation in a concentration-dependent manner. It was found that genistein treatment markedly decreased the levels of COX-2 mRNA and protein expression without significant changes in the expression of COX-1, which was correlated with a decrease in $PGE_2$ synthesis. Genistein treatment also partly inhibited the levels of human telomerase reverse transcriptase (hTERT) as well as human telomerase RNA (hTR) and telomerase-associated protein (TEP)-1, and the activity of telomerase. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of genistein.

• Fisheries and Aquatic Sciences
• /
• v.19 no.9
• /
• pp.37.1-37.5
• /
• 2016

Human immunodeficiency virus (HIV) is the causative agent of acquired immune deficiency syndrome (AIDS). Anti-HIV agents targeting various steps in HIV life cycle have been developed; however, so far, no effective drugs have been found. We show here that a peptide isolated from Spirulina maxima (SM-peptide) inhibits HIV-1 infection in a human T cell line MT4. SM-peptide inhibited $HIV-1_{IIIB}$-induced cell lysis with a half-maximal inhibitory concentration ($IC_{50}$) of 0.691 mM, while its 50 % cytotoxic concentration ($CC_{50}$) was greater than 1.457 mM. Furthermore, the SM-peptide inhibited the HIV-1 reverse transcriptase activity and p24 antigen production. This suggests that SM-peptide is a novel candidate peptide, which may be developed as a therapeutic agent for acquired immunodeficiency syndrome patients.

• Korean Journal of Human Ecology
• /
• v.2 no.1
• /
• pp.52-67
• /
• 1999

The primary purpose of this study was to analyze the types of home managers in association with effects of resourcefulness, communication and the conditions for effective home management＇ proposed by Shape and Winter(1991). A survey was conducted by subjects of 300 wives in Kwangju city. The statistical data were obtained by cluster analysis, one-way ANOVA, Duncan＇s test and chi-square test. Manager types based on the four conditions, resourcefulness and communication were classified into four clusters. Educational background, job status, family types, family life cycle and age difference between the oldest and the youngest child of the subjects were different from manager types. In conclusion, this study identified the four conditions of performance-condition dichotomy, timing, high level and middle range, resourcefulness, and communications in order to develop managerial capability. (Korean J Human Ecology 2(1) : 52∼67, 1999)

• Korean Journal of Human Ecology
• /
• v.2 no.1
• /
• pp.25-37
• /
• 1999

The purposes of this research were to find out general trends of employed women's stress and to investigate the differences and effects of related variables. The data were obtained through 239 employed women living in Kwangju and Chonnam. The major findings were as follows : 1) Employed women's scores of stressor, stress cognition and distress were under medium but coping scores were relatively high. 2) Significant differences in employed women's stressors were found according to age, income, job satisfaction, and family life cycle. Stress cognition differed according to job adjustment conditions and self-esteem. Also distress related with job conditions and object. Stress coping levels were different according to income, job conditions, self-esteem, and sex-role attitude. 3) In analyzing the causal effects among related variables, employed women's stressors were affected by job satisfaction and age. Also stress cognition were influenced by job satisfation and self-esteem. Job conditions showed significant effects on distress and self-esteem, sex-role attitudes showed on coping. Conclusively job satisfaction, self-esteem and gender equality were most important variables for employed women's stress. (Korean J of Human Ecology 2(1) : 25-37 1999)