Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

A Spirulina maxima-derived peptide inhibits HIV-1 infection in a human T cell line MT4

  • Jang, In-Seung (Department of Chemistry, Pukyong National University) ;
  • Park, Sun Joo (Department of Chemistry, Pukyong National University)
  • Received : 2016.08.23
  • Accepted : 2016.11.05
  • Published : 2016.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Human immunodeficiency virus (HIV) is the causative agent of acquired immune deficiency syndrome (AIDS). Anti-HIV agents targeting various steps in HIV life cycle have been developed; however, so far, no effective drugs have been found. We show here that a peptide isolated from Spirulina maxima (SM-peptide) inhibits HIV-1 infection in a human T cell line MT4. SM-peptide inhibited $HIV-1_{IIIB}$-induced cell lysis with a half-maximal inhibitory concentration ($IC_{50}$) of 0.691 mM, while its 50 % cytotoxic concentration ($CC_{50}$) was greater than 1.457 mM. Furthermore, the SM-peptide inhibited the HIV-1 reverse transcriptase activity and p24 antigen production. This suggests that SM-peptide is a novel candidate peptide, which may be developed as a therapeutic agent for acquired immunodeficiency syndrome patients.

Keywords

References

  1. Ay E, Banati F, Mezei M, Bakos A, Niller HH, Buzas K, et al. Epigenetics of HIV infection: promising research areas and implications for therapy. AIDS Rev. 2013;15:181-8.
  2. Broder S. The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic. Antiviral Res. 2010;85:1-18. https://doi.org/10.1016/j.antiviral.2009.10.002
  3. Lifson MA, Ozen MO, Inci F, Wang S, Inan H, Baday M, et al. Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring. Adv Drug Deliv Rev. 2016;103:90-104. https://doi.org/10.1016/j.addr.2016.05.018
  4. Migueles SA, Connors M. Success and failure of the cellular immune response against HIV-1. Nat Immunol. 2015;16:563-70. https://doi.org/10.1038/ni.3161
  5. Ngo DH, Vo TS, Ngo DN, Wijesekara I, Kim SK. Biological activities and potential health benefits of bioactive peptides derived from marine organisms. Int J Biol Macromol. 2012;51:378-83. https://doi.org/10.1016/j.ijbiomac.2012.06.001
  6. Oku N, Gustafson KR, Cartner LK, Wilson JA, Shigematsu N, Hess S, et al. Neamphamide A, a new HIV-inhibitory depsipeptide from the Papua New Guinea marine sponge Neamphius huxleyi. J Nat Prod. 2004;67:1407-11. https://doi.org/10.1021/np040003f
  7. Plaza A, Gustchina E, Baker HL, Kelly M, Bewley CA. Mirabamides A-D, depsipeptides from the sponge Siliquariaspongia mirabilis that inhibit HIV-1 fusion. J Nat Prod. 2007;70:1753-60. https://doi.org/10.1021/np070306k
  8. Plaza A, Bifulco G, Keffer JL, Lloyd JR, Baker HL, Bewley CA. Celebesides A-C and theopapuamides B-D, depsipeptides from an Indonesian sponge that inhibit HIV-1 entry. J Org Chem. 2009;74:504-12. https://doi.org/10.1021/jo802232u
  9. Vo TS, Kim SK. Potential anti-HIV agents from marine resources: an overview. Mar Drugs. 2010;8:2871-92. https://doi.org/10.3390/md8122871
  10. Vo TS, Ngo DH, Kang KH, Park SJ, Kim SK. The role of peptides derived from Spirulina maxima in downregulation of FcepsilonRI-mediated allergic responses. Mol Nutr Food Res. 2014;58:2226-34. https://doi.org/10.1002/mnfr.201400329
  11. Zampella A, Sepe V, Luciano P, Bellotta F, Monti MC, D'Auria MV, et al. Homophymine A, an anti-HIV cyclodepsipeptide from the sponge Homophymia sp. J Org Chem. 2008;73:5319-27. https://doi.org/10.1021/jo800583b

Cited by

  1. Novel Antiretroviral Structures from Marine Organisms vol.24, pp.19, 2016, https://doi.org/10.3390/molecules24193486
  2. Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds vol.10, pp.6, 2016, https://doi.org/10.3390/antibiotics10060746