• Title/Summary/Keyword: homogeneous weight

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Purification and Characterization of Raw Starch-Digesting Enzyme from Rhizopus oryzae (Rhizopus oryzae가 생성하는 생전분 분해효소의 정제 및 특성)

  • Kim, Chan-Jo;Oh, Man-Jin;Lee, Jong-Soo
    • Korean Journal of Food Science and Technology
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    • v.18 no.4
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    • pp.288-293
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    • 1986
  • A raw starch-digesting enzyme from Rhizopus oryzae was purified by ammonium sulfate fractionation, DEAE-sephacel column chromatography and Sephadex G-150 gel filtration. The specific activity of purified enzyme was 45.2 Ulmg protein and the yield was 16.2%. The purified enzyme was found to be homogeneous bypolyacrylamide gel electrophoresis and its molecular weight was estimated to be 67,000 by SDS-polyacrylamide gel electrophoresis, and also the enzyme had Km value of 4.082 mg/ml for raw corn starch. The optimal temperature and pH for the enzyme activity were $50^{\circ}C$ and 4.0-5.0, respectively. Reaction product of raw corn starch by purified enzyme was glucose mainly.

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Densification Behaviour of Magnesium Powders during Cold Isostatic Pressing using the Finite Element Method (유한요소법을 이용한 마그네슘 분말의 냉간정수압 공정시 치밀화 거동 해석)

  • Yoon, Seung-Chae;Kwak, Eun-Jeong;Choi, Won-Hyoung;Kim, Hyoung-Kun;Kim, Taek-Soo;Kim, Hyoung-Seop
    • Journal of Powder Materials
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    • v.14 no.6
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    • pp.362-366
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    • 2007
  • Magnesium and magnesium alloys are promising materials for light weight and high strength applications. In order to obtain homogeneous and high quality products in powder compaction and powder forging processes, it is very important to control density and density distributions in powder compacts. In this study, a model for densification of metallic powder is proposed for pure magnesium. The mode] considers the effect of powder characteristics using a pressure-dependent critical density yield criterion. Also with the new model, it was possible to obtain reasonable physical properties of pure magnesium powder using cold iso-state pressing. The proposed densification model was implemented into the finite element method code. The finite element analysis was applied to simulating die compaction of pure magnesium powders in order to investigate the density and effective strain distributions at room temperature.

Purification of Internal Invertase in Rhodosporidium toruloides Mating Type A Cells (Rhodosporidium toruloides의 접합형 A 세포내 Invertase의 정제)

  • Jeong, Yong-Kee;Ryu, Beung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.6
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    • pp.725-730
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    • 1992
  • The internal invertase was purified from cell free extract of Rhodosporidium toruloides IFO 0559-M-919 by acid precipitation, ion-exchange chromatography and gel filtration to the unique enzyme protein on disc electrophoresis. We have found out that molecular weight of purified internal invertase was 90,000 by gel filtration and the purified enzyme was protein with 4 homogeneous subunits appearing as single band of 22,000daltons on SDS-polyacrylamide gel electrophoresis.

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Shell Powder Coating on the Surface of Concrete by Geopolymer Cement (지오폴리머 시멘트를 이용한 콘크리트 표면의 패각 분말 코팅)

  • Kim, Gab-Joong;Han, Hyun-Geun;Seo, Dong-Seok;Lee, Jong-Kook
    • Korean Journal of Materials Research
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    • v.20 no.1
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    • pp.1-6
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    • 2010
  • Geopolymer materials are attractive as inorganic binders due to their superior mechanical and eco-friendly properties. In the current study, geopolymer-based cement was prepared using aluminosilicate minerals from fly-ash with KOH as an alkaline-activator and $Na_2SiO_3$ as liquid glass. Then, calcium carbonate powder from a clam shell was mixed with the geopolymer and the mixture was coated on a concrete surface to provide points of attachment for environmental organisms to grow on the geopolymers. We investigated the effect of the shell powder grain size on the microstructure and bonding property of the geopolymers. A homogeneous geopolymer layer coated well on the concrete surface via aluminosilicate bonding, but the adhesiveness of the shell powder on the geopolymer cement was dependent on the grain size of the shell powder. Superior adhesive characteristics were shown in the shell powder of large grain size due to the deep penetration into the geopolymer by their large weight. This kind of coating can be applied to the adhesiveness of eco-materials on the surface of seaside or riverside blocks.

PREPARATION, PHYSICAL PROPERTIES AND MODEL ANALYSIS OF LINEAR HOMOGENEOUS POLYURETHANE

  • Laporta, M.;Pegoraro, M.;Bianchi, F.;Consolati, G.;Zanderighi, L.
    • Proceedings of the Membrane Society of Korea Conference
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    • 2003.07a
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    • pp.81-85
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    • 2003
  • PU block polymers are constituted by sequences of constitutional units CU which are : a diisocyanate T, a polyol or a polyamine V, an extensor E .Due to the CU different solubility parameters ,hard and soft micro domains are formed;they can aggregate into different phases. A linear PU can be described by two parameters R=T/N and $R_{E}$=(R-1)V/E.A stoichiometric model is proposed which gives the general formula of all the possible PU: X((TV)$_{k}$(TE)$_{n}$)$_{m}$X , where k and m can be calculated from the experimental molecular weights respectively of pre polymer and polymer and n= R-1.Physical properties depend on PU composition and on nature of CU. I.R. analysis is useful for discovering the hydrogen bonds location : DSC gives informations on the presence of the different phases .PALS analysis shows that nano cavities become tighter on increasing n. A series of particular Pus ,obtained from :TDI, a low molecular weight polyol, a cyclic extensor, at different R and RE was prepared and analysed. These PU are amorphous, monophasic and appear to be good candidates for membranes.nes.nes.

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Optimal Battery Pack Design Tool for the Delivery UAV (배송용 무인항공기를 위한 최적 배터리팩 설계 툴)

  • Jung, Sunghun;Jeong, Heon
    • Journal of the Korea Convergence Society
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    • v.8 no.6
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    • pp.219-226
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    • 2017
  • As the UAV industry is getting matured, various types of UAVs have appeared in many application fields, including filming, reconnaissance, rescue, and etc. and it requires the quick hardware designs, particularly a battery pack, of the UAV. We developed the automatic battery pack design tool for the convenient battery pack configuration design of the hoverable type delivery UAV. With inputs, including current profile, voltage profile, various kinds of cell specifications, desired battery pack voltage, and etc. the automatic battery pack design tool calculates a pack having the minimum weight and the maximum capacity by combining either homogeneous cells or heterogeneous cells. Also, the tool could predict the capacity fading trend of the designed battery pack configuration.

Development of Reduced Normal Dodecane Chemical Kinetics (축소 노멀 도데케인 화학반응 메커니즘 개발)

  • Lee, Sangyul;Kim, Gyujin;Min, Kyoungdoug
    • Transactions of the Korean Society of Automotive Engineers
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    • v.21 no.2
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    • pp.37-44
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    • 2013
  • Generally, a reduced chemical mechanism of n-heptane is used as chemical fuel of a 3-D diesel engine simulation because diesel fuel consists of hundreds of chemical components and various chemical classes so that it is very complex and large to use for the calculation. However, the importance of fuel in a 3-D simulation increases because detailed fuel characteristics are the key factor in the recent engine research such as homogeneous charged compression ignition engine. In this study, normal paraffin, iso paraffin and aromatics were selected to represent diesel characteristics and n-dodecane was used as a representative normal paraffin to describe the heavy molecular weight of diesel oil (C10~C20). Reduced kinetics of iso-octane and toluene which are representative species of iso paraffin and aromatics respectively were developed in the previous study. Some species were selected based on the sensitivity analysis and a mechanism was developed based on the general oxidation scheme. The ignition delay times, maximum pressure and temperature of the new reduced n-dodecane chemical mechanisms were well matched to the detailed mechanism data.

CD5+/CD21-Chronic Lymphocytic Leukemia in a Cat

  • Choi, Sorin;Bae, Hyeona;Chun, Daseul;Kim, Jihu;Shin, Sun Woo;Cho, ARom;Jung, Dong-In;Yu, DoHyeon
    • Journal of Veterinary Clinics
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    • v.37 no.6
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    • pp.350-354
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    • 2020
  • Feline chronic lymphocytic leukemia (CLL) is a rare disease. Its diagnosis is not simple because of the absence of clinical signs and the presence of mature lymphocytosis. An 11-year-old female spayed Russian Blue cat was referred to the veterinary medical teaching hospital for lethargy, diarrhea, weight loss, and inappetence. Marked lymphocytic leukocytosis and a significantly increased number of small-to-intermediate-sized lymphocytes in the peripheral blood were found on hematological examination. The results of the feline leukemia virus and immunodeficiency virus test were negative. Further, mild splenomegaly was detected. Bone marrow aspirate analysis revealed mature lymphocytosis and a clonally rearranged T cell receptor gene with the polymerase chain reaction (PCR) for antigen receptor rearrangement assay. Flow cytometric immunophenotyping showed a homogeneous population of CD5+/CD21-T-cells in the peripheral blood and bone marrow. According to the results of the aforementioned examinations, CLL was diagnosed. Treatment was not initiated at the time of diagnosis because the clinical signs were mild and did not affect the quality of life. This report describes the clinical findings and use of advanced diagnostic tools such as molecular clonality analysis and immunophenotyping for the diagnosis of feline CLL.

Production and investigation of 3D printer ABS filaments filled with some rare-earth elements for gamma-ray shielding

  • Batuhan Gultekin;Fatih Bulut;Hatice Yildiz;Hakan Us;Hasan Ogul
    • Nuclear Engineering and Technology
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    • v.55 no.12
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    • pp.4664-4670
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    • 2023
  • Radiation is the main safety issue for almost all nuclear applications, which must be controlled to protect living organisms and the surrounding materials. In this context, radiation shielding materials have been investigated and used in nuclear technologies. The choice of materials depends on the radiation usage area, type, and energy. Polymer materials are preferred in radiation shielding applications due to their superior characteristics such as chemical inertness, resistivity, low weight, flexibility, strength, and low cost. In the presented work, ABS polymer material, which is possibly the most commonly used material in 3D printers, is mixed with Gd2O3 and Er2O3 nanoparticles. ABS filaments containing these rare-earth elements are then produced using a filament extruder. These produced filaments are used in a 3D printer to create shielding samples. Following the production of shielding samples, SEM, EDS, and gamma-ray shielding analyses (including experiments, WinXCOM, GEANT4, and FLUKA) are performed. The results show that 3D printing technology offers significant enhancements in creating homogeneous and well-structured materials that can be effectively used in gamma-ray shielding applications.

Characteristics of Foam Concrete with Application of Mineral Admixture (무기혼화재 적용에 따른 기포콘크리트의 특성)

  • Kim, Sang-Chel;Kim, Yun-Tae
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.4 no.2
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    • pp.97-106
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    • 2009
  • self-loading, various problems related to construction can be solved as well as the save of construction cost. Thus, this study has an aim of applying foam concrete to structural purpose by adding bottom ash as a reinforcing material like fine aggregate, in contrast to conventional non-structural usage such as soundproofing or insulating materials. In addition, it was evaluated in terms of unit volume weight, flow value, air void, water absorption and dosage of foam agent wether replacement of cement by granulated blast furnace slag or fly-ash has an effect on the material characteristics of foam concrete. As results of experiments, it can be found that the increase of fine aggregate ratio, that is to say, the increase of bottom ash results in the increase of unit volume weight, while decreasing air void and flow value. But, appropriate addition of bottom ash to foam concrete makes it easy to control a homogeneous and uniform quality in foam concrete due to less sensitive to bubbles. As the replacement ratio of mineral admixtures such as granulated blast furnace slag and fly-ash increases, as unit volume weight tends to decrease. In the meanwhile, serious effects were shown on fluidity of foam concrete when more than limit of replacement ratio was applied.

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