• 한국발생생물학회지:발생과생식
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• 제4권2호
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• pp.195-201
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• 2000

점농어, Lateolabrax maculatus의 성분화 과정을 밝히기 위해 부화 직후부터 365일령까지 생식소의 분화 및 발달을 조사하였다. 원시생식세포와 생식융기는 부화 후 30일령(전장: 11.7~13.2 mm)에 나타났으며,40일령 자어(12.5~14.0 mm)에서 서로 융합되어 미분화 생식소를 형성하였다. 60일령 치어(23.6~27.0 mm)에서는 생식소 양쪽 끝의 체세포조직이 분열ㆍ신장되어 난소의 분화가 개시되었고, 80일령 치어(33.1~42.5 mm)에서는 완전한 난소강이 나타났다. 70일령 치어(24.8~31.6 mm)에서는 생식소 중앙에 정소관 원기가 출현하여 정소의 분화가 시작되었다. 168일령 치어(88.0~l15.4 mm)의 난소내 생식세포는 감수분열을 시작하였으며, 287일령 치어(175.1 ~233.6 mm)에서는 염색 인기와 주변인기 의 난모세포가 출현하였다. 245일령 치어(124.4~168.3 mm)에서는 정소내 생식세포의 감수분열이 시작되었고, 365일령 치어(162.5~253.8 mm)의 세정관은 정자로 충만되었다 암ㆍ수 성비는 1:1.38이었으며 성분화 양상은 자웅이체형 중 분화형이었다.

• 대한한방내과학회지
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• 제30권2호
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• pp.270-287
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• 2009

Objective : Yinjinchunggan-tang(YJCGT) is reported previously as having theraputic effects on hepatitis such as anti-implammatory, anti-apoptotic, anti-viral(HBV), etc. Though this prescription is not studied on its anti-fibrogenic effect, it is still expected to have the effect in the liver. Thus, this study was performed to investigate the anti-fibrogenic effect of YJCGT on thioacetamide(TAA)-induced liver fibrosis in rats. Method: Rat liver fibrosis was induced by intraperitoneal TAA injection(150mg/kg) 3 times a week for 5 weeks. After the YJCGT (YJCGT 1g/kg, YJCGT 2g/kg)-treatment, body weight, liver and spleen weights, liver function test, the complete blood count and the portal pressure were studied. In addition, gene expressions of ASMA, procollagen type Ia2, MMP2, TIMP1 and TIMP2, all of which are known to be associated with liver fibrosis, were analyzed by RT-PCR. After YJCGT (YJCGT 1g/kg, YJCGT 2g/kg) treatment, percentages of collagen in TAA-induced rat liver tissue were measured by image analyzer. Results : The body weight of the normal group increased more than that of the control and YJCGT-treated groups. The AST level of the YJCGT lg/kg-treated group significantly decreased compared to that of the control. The ALT and the GGT levels of the YJCGT 2g/kg-treated group significantly increased compared to those of the control. In the YJCGT-treated groups. WBC, RBC and Hgb elevated by TAA injection decreased but platelet count increased. In the YJCGT lg/kg-treated group, the portal pressure elevated by TAA injection significantly decreased. The significant decreases in the gene expressions of procollagen type Ia2, MMP2 and TIMP2 were observed in the YJCGT-treated groups. In histological findings. TAA injections caused severe liver fibrosis, but the YJCGT treatment significantly reduced the amounts of hepatic collagens. Conclusions: These results suggest that YJCGT has beneficial effects on the treatment of patients with liver cirrhosis as well as chronic hepatitis. Further study should be done to decide the optimal concentration of the YJCGT for the treatment of liver cirrhosis.

• 동의생리병리학회지
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• 제17권6호
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• pp.1453-1462
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• 2003

In order to study the effect of oral administration of Mawhangyounpye-tang against to asthma, astham was induced to allergy-sensitive Balb/c mouse with ovalbumin using method of Hatfield et al (1997). The changes of diameter lumen of upper portion of the trachea, lung weight, gross appearance of lung, histological profiles of lung and trachea, numbers of cellular compartments in the bronchoalveolar lavage fluid (BALF), numbers and morphology of the mast cells in the trachea, numbers of mucus-secretory cell in the broncus, morphology of the bronchus, ultramicroscopical appearance of surface of trachea and number of cilia and mucous-secretory cells by scanning electron microscope. Obtained results were as follows. 1. The diameters of trachea lumen were significantly decreased in asthma induced control groups and these decreasing were result from hypertrophy of mucous membrane. However, these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 2. Lung weights and black spots, which were result from infiltration of inflammatory cells, were significantly increased in asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 3. Hypertrophy of mucous membrane of trachea and bronchus and !bronchioles in the lung, peritracheal, peribronchus and peribronchiolar inflammatory cell infiltration, and mucoid exudate deposit in the lumen were observed in asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 4. Cellular compartments including neutrophil and eosinophil were dramatically increased in the BALF of asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 5. Mast cell degranulation and decreasing of the numbers of mast cells were detected in the trachea of asthma induced control groups. However, these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 6. Shed, decreasing of cilia cell and increasing of mucous-secretory cells in the surface of the trachea of asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. In conclusion, it Is considered that Mawhangyounpye-tang has somewhat favorable effect on the asthma because the asthma specific series of abnormalities in respiratory system were decreased after oral administratin of Mawhangyounpye-tang in this study. In future, it is needed that the toxicological and dosagespecific study of Mawhangyounpye-tang to use against asthma with safe.

• 한국임상수의학회지
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• 제28권4호
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• pp.452-456
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• 2011

5 year-old female Siberian husky which was 27 kg had presented with a recurrent rhinitis with chronic discharge and cough. A nasal foreign material had been suggested by a finding of a bone density ($0.3{\times}0.3$ cm) in the left nasal cavity on X-ray and CT-scanning. Soft tissue opacity in frontal sinus and nasal cavity was increased and foreign material was located beside turbinate bone in the left nasal. We found that there was the increase in the number of eosinophil and mast cell by the nasal cytology test. These results mentioned above indicated that the rhinitis by nasal foreign body was suspicious. We decided that the transfrontal rhinotomy could be the proper procedure to approach the material in this case. After rhinotomy, the foreign body and severe sticky discharge were removed. Drain was placed through the hole and into the frontal sinus and nasal cavity which were flushed two times a day for 7 days. The clinical signs such as cough and nasal discharge were shown to be improved in the every visiting for the re-check. On the $40^{th}$ day after surgery, we could confirm that the most of soft tissue density in the frontal sinus and nasal cavity was decreased by CT-scanning. However, foreign body was not identified by histological examination. For the treatment of chronic rhinitis caused by foreign body, the surgical method such as rhinotomy can be applied, when it is difficult to remove it in the guide of the nasal endoscope.

• 대한수의학회지
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• 제41권4호
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• pp.467-475
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• 2001

Cardiac arrest, hypoxia, shock or seizure has been known to induce cerebral ischemia. This study was designed to investigate the effect of ischemia on hippocampal pyramidal layer induced by transient bilateral occlusion of the common carotid arteries. Mature Mongolian gerbils were sacrificed at days 2, 4, and 7 after carotid occlusion for 10 minutes. Sham-operated gerbils of control group were subjected to the same protocol except for carotid occlusion. During operation for ischemia, body temperature was maintained $37{\pm}0.5^{\circ}C$ in all gerbils. Paraffin-embedded brain tissue blocks were cut into coronal slices and stained with H-E stain or immunostain by TUNEL method. Neurons with the oval and prominent nucleus and without the eosinophilic cytoplasm in the subfield of hippocamapal pyramidal layer were calculated as to be viable neurons. Their chromatins were condensed or clumped. Their nuclei appeared multiangular or irregularly shrinked. The width of the pyramidal layer was reduced due to the loss of nuclei. At day 2 after reperfusion, some neurons in the CA1 subfield were slightly eosinophilic. But most neurons in the CA2 subfield were strongly eosinophilic. At day 4 day, most neurons in the CA1 subfield were severely damaged and at day 7 day, only a few survived neurons were observed. Survived neurons per longitudinal 1mm sector in the CA1, CA2, CA3, and CA4 subfields of pyramidal layer were investigated. At day 2, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 104.5/mm (54.3%), 51.0/mm (33.8%), 105.5/mm (85.6%), and 124.3/mm (93.5%) compared to the nonischemic control group, respectively. At day 4, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfields were 3.2/mm (1.7%), 51.5/mm(34.2%), 95.3/mm (77.4%), and 112.5/mm (84.6%), respectively. At day 7, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 0.8/mm (0.4%), 5.7/mm(3.8%), 9.8/mm (8.0%), and 5.0/mm (3.7%), respectively. The mean numbers of apoptotic positive neurons in the CA1 subfield at day 2, 4, and 7 after reperfusion were 67.8/mm, 153.2/mm and 123.7/mm, respectively. These results suggest that the transient cerebral ischemia cause severe damages in most neurons at day 7 and that the prosminent apoptotic positive neurons in hippocampal pyramidal layer are the delayed neuronal death induced by ischemia.

• 폴리머
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• 제27권3호
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• pp.226-234
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• 2003

이프리플라본은 이소플라본의 파생물로서 골의 재흡수를 방지하여 골의 재형성을 방해함으로써 골 형성에 도움을 준다. 이프리플라본은 칼슘의 양을 안정적으로 증가시킴과 함께 골수 줄기 세포의 작용으로 세포층에 칼슘을 침착시킨다. 조직공학적 골을 형성시키기 위해 락타이드-글리를라이드 공중합체 (PLGA)에 이프리플라본을 함유시킨 지치체를 용매 캐스팅/염 추출법으로 제조하였다. 수은 다공도계, 주사 전자 현미경, 시차 주사 열량계, X선 회절기를 이용하여 특성결정을 수행하였고, 이프리플라본이 함유된 지지체와 이프리플라본이 함유되지 않은 지지체를 면역이 결핍된 쥐의 피하에 삽입하여 이들의 골 형성을 비교하였다. 조직을 hematoxylin & eosin, 본쿠사 염색과 면역화학적 염색법인 콜라겐 I 형과 오스테오칼신 염색을 하였다. 이프리플라본이 함유된 담체의 다공도는 91.7% 이상이었고, 평균 다공 크기도 101 $\mu\textrm{m}$였다. PLGA로만 제조된 지지체와 이프리플라본을 50% 함유시킨 지지체를 동물 실험을 수행한 결과 이프리플라본은 피하 층과 다른 연조직에서 미분화 줄기 세포가 칼슘 침착, 골아 세포, 골상으로의 유도에 더 많은 영향을 주는 것을 관찰하였다. 결론적으로 이프리플라본을 함유한 지지체에서 이프리플라본이 골형성에 중요한 요인으로 작용한다고 사료된다.

• 한국양식학회지
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• 제19권1호
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• pp.19-24
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• 2006

감성돔 아가미에 기생하는 Alella의 형태 및 조직학적 구조를 관찰하고, 삼투압 충격에 의한 기생충의 구제 가능성을 조사하였다. 숙주당 Alella의 기생수는 평균 5개체였으며, 모든 개체가 암컷이었다. 이들은 아가미 연골조직에 bulla를 삽입하여 몸을 고정하고 있었다. 이 기생충의 몸은 두흉부$(1825.0{\pm}495.8{\mu}m)$, 몸통$(1822.1{\pm}521.5{\mu}m)$ 부착기인 bulla $(134.2{\pm}43.1{\mu}m)$ 구성되어져 있었다. 기생충은 아가미 2차새변을 섭취하였으며, 이로 인해 감성돔은 출혈과 빈혈이 발생하였고, 숙주의 폐사는 빈혈에 의한 것으로 추정된다. 감성돔에 기생한 Alella성체는 염분농도 $0{\sim}15\%_{\circ}$에서 48시간 이내에 100% 폐사하여, 삼투압 충격에 의하여 구제할 수 있는 것으로 판단되었다.

• 대한관절경학회지
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• 제6권2호
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• pp.161-170
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• 2002

목적 : 관절경을 이용한 거골 박리성 골연골염의 단순 절제술 후 그 결과를 알아보고 이차 관절경을 통하여 결손 부위의 육안 및 조직학적 변화를 알아보고자 하였다. 대상 및 방법 : 관절경으로 유리체 및 괴사 골 조직을 제거한후 출혈되는 기저부를 남겨두고 치료한 22예를 대상으로 하였으며, 임상적 및 기능적 평가를 하여 수술의 유용성을 알아보았다. 7예에서 이차 관절경을 술 후 3개월에서 8년 사이에 시행하여 병변 부위의 관찰 및 타 부위의 이차적인 병변을 관찰하였으며, 생검을 시행하여 결손부 재생 조직에 대한 조직학적 검사를 시행하였다. 추시 관찰은 평균 42개월(14개월$\~$8년) 이었다. 결과 : 최종 추시 관찰에서 Ankle-hindfoot(100점) 점수는 술 전 67점에서 술 후 82점으로 의미있는 호전을 보였고(p<0.003), 주관적 및 기능적 평가(100점)상 술 후 평균 82점으로 만족할 만한 결과를 얻었다. 이차 관절경상 결손부는 섬유성 조직으로 채워지는 양상을 보였으며, 시간의 경과에 따라 섬유성 조직보다는 섬유 연골 조직이 증가되는 소견을 보였다. 술 후 특이한 이차적인 병변의 발생은 관찰되지 않았다. 결론 : 관절경을 이용한 거골 박리성 골연골염에 대한 단순 절제술은 결손 부위를 남겨 놓고 치료하는 방법이나 이차적 병변의 발생없이 결손부가 채워지는 소견을 보여 유용한 방법으로 사료되었다.

• 한국수산과학회지
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• 제30권4호
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• pp.627-633
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• 1997

1995년 11월부터 1996년 10월까지 부산 수영만 인근해역에서 채집된 쭈굴감펭, Scorpaena miestoma의 생식소 구조, 생식세포의 발달 및 생식주기 등이 광학현미경 조직표본을 기초로 연구되었다. 정소의 내부조직상은 다수의 곡정세관으로 구성된다. 각각의 곡정세관은 여러개의 소낭 구조를 가지는데 각 소낭내의 생식세포들은 같은 단계의 발달상태를 보인다. 난소내부는 난소외막으로 부터 시작된 여러장의 난소박판으로 구성되어 있다. 난원세포는 난소박판의 내부상피에서 유래하며, 난모세포로 성장하면서 난소강쪽으로 돌출되어 난병에 의해 난소박판에 연결된다. 생물학적최소형은 암${\cdot}$수 모두 전장 12.5cm로 조사 되었다. 생식소숙도지수는 암 (3.81)${\cdot}$수(0.23) 모두 10월에 연중 최고치를 나타냈다. 암컷의 생식주기는 성장기 ($5\~8$월), 성숙기 ($9\~10$월), 완숙 및 산란기 ($11\~12$월) 그리고 회복 및 휴지기 ($1\~4$월)로 나눌 수 있다. 수컷의 생식주기는 성장기 ($6\~8$월), 성숙기 ($9\~10$월), 완숙 및 방정기 ($11\~l$월) 그리고 회복 및 휴지기 ($2\~5$월)로 나눌 수 있다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권6호
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• pp.520-530
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• 2006

Undifferentiated mesencymal stem cells(UMSCs) have been thought to be multipotent cells that can replicate as undifferentiated cells and that have the potential to differentiate into lineages of mesenchymal tissue including the bone, cartilage, fat, tendon, muscle, and marrow stroma. It can be used to sinus lifting, Guided bone regeneration, other bone graft in dental part. The purpose of this study is to evaluate the effect of mesencymal stem cells on sinus augmentation with autogenous bone, fibrin glue mixture in a rabbit model. 8 New Zealand white rabbits were divided randomly into 4 groups based on their time of sacrifice(1, 2, 4 and 8 weeks). First, undifferentiated mesenchymal stem cells were isolated from iliac crest marrow of rabbits and expanded in vitro. cell culture was performed in accordance with the technique described by Tsutsumi et al. In the present study, The animals were sacrificed at 1, 2, 4 and 8 weeks after transplantation, and the bone formation ability of each sides was evaluated clinically, radiologically, histologically and histomorphologically. According to the histological observations, Stem cell group showed integrated graft bone with host bone from sinus wall. At 2 and 4weeks, It showed active newly formed bone and neovascularization. At 8 weeks, lamella bone was observed in sinus graft material area. Radiologically, autobone with stem cell showed more radiopaque than autobone without stemcell. there were significant differences in bone volume between 2 and 4 weeks (p<0.05). In summary, the autobone with stem cells had well-formed, newly formed bone and neovasculization, compared with the autobone without stem cells (esp. 2 weeks and 4 weeks) The findings of this experimental study indicate that the use of a mixture of mesenchymal stem cell yielded good results in osteogenesis and bone volume comparable with that achieved by autogenous bone. Therefore, this application of this promising new sinus floor elevation method for implants with tissue engineering technology deserves further study.