Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

THE EFFECTS OF UNDIFFERENTIATED MESENCHYMAL STEM CELLS ON SINUS BONE GRAFTING IN RABBIT

가토의 상악동 골이식술시 미분화 간엽 줄기세포의 골형성 효과

  • Oh, Sung-Hwan (Department of Oral & Maxillofacial Surgery, School of Dentistry, Wonkwang University) ;
  • Choi, Young-Won (Department of Oral & Maxillofacial Surgery, School of Dentistry, Wonkwang University) ;
  • Kim, Bum-Soo (Department of Oral & Maxillofacial Surgery, School of Dentistry, Wonkwang University) ;
  • Yeo, In-Bum (Department of Oral & Maxillofacial Surgery, School of Dentistry, Wonkwang University) ;
  • Jo, Pil-Kwy (Department of Oral & Maxillofacial Surgery, School of Dentistry, Wonkwang University)
  • 오승환 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 채영원 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 김범수 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 여인범 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 조필귀 (원광대학교 치과대학 구강악안면외과학교실)
  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Undifferentiated mesencymal stem cells(UMSCs) have been thought to be multipotent cells that can replicate as undifferentiated cells and that have the potential to differentiate into lineages of mesenchymal tissue including the bone, cartilage, fat, tendon, muscle, and marrow stroma. It can be used to sinus lifting, Guided bone regeneration, other bone graft in dental part. The purpose of this study is to evaluate the effect of mesencymal stem cells on sinus augmentation with autogenous bone, fibrin glue mixture in a rabbit model. 8 New Zealand white rabbits were divided randomly into 4 groups based on their time of sacrifice(1, 2, 4 and 8 weeks). First, undifferentiated mesenchymal stem cells were isolated from iliac crest marrow of rabbits and expanded in vitro. cell culture was performed in accordance with the technique described by Tsutsumi et al. In the present study, The animals were sacrificed at 1, 2, 4 and 8 weeks after transplantation, and the bone formation ability of each sides was evaluated clinically, radiologically, histologically and histomorphologically. According to the histological observations, Stem cell group showed integrated graft bone with host bone from sinus wall. At 2 and 4weeks, It showed active newly formed bone and neovascularization. At 8 weeks, lamella bone was observed in sinus graft material area. Radiologically, autobone with stem cell showed more radiopaque than autobone without stemcell. there were significant differences in bone volume between 2 and 4 weeks (p<0.05). In summary, the autobone with stem cells had well-formed, newly formed bone and neovasculization, compared with the autobone without stem cells (esp. 2 weeks and 4 weeks) The findings of this experimental study indicate that the use of a mixture of mesenchymal stem cell yielded good results in osteogenesis and bone volume comparable with that achieved by autogenous bone. Therefore, this application of this promising new sinus floor elevation method for implants with tissue engineering technology deserves further study.

Keywords

References

  1. Block MS, Kent JN : Sinus augmentation for dental implants: the use of autogenous bone. J Oral Maxillofac Surg 55 : 1281, 1997 https://doi.org/10.1016/S0278-2391(97)90185-3
  2. Leonardis D, Pecora GE : Augmentation of the maxillary sinus with calcium sulfate: one year clinical report from a prospective longitudinal study. Int J Oral Maxillofac Implants 14 : 869, 1999
  3. Tatum H Jr : Maxillary and sinus implant reconstructions. Dent Clin North Am 30 : 207, 1986
  4. Boyne PJ, James RA : Grafting of the maxillary sinus floor with autogenous marrow and bone. J Oral Maxillofac Surg 38 : 613, 1980
  5. Lind M : Growth factors: Possible new clinical tools. Acta Orthop Scand 67 : 407, 1996 https://doi.org/10.3109/17453679609002342
  6. Cawood JI, Howell RA : A classification of the edentulous jaws. Int J Oral Maxillofac Surg 17 : 232, 1996 https://doi.org/10.1016/S0901-5027(88)80047-X
  7. Garg Ak : The future role of growth factors in bone grafting. Dent Implantol Update 10 : 5, 1999
  8. Ohgushi H, Goldberg VM, Caplan AI : Heterotopic osteogenesis in porous ceramics induce by marrow cells. J Orthop Res 7 : 568, 1989 https://doi.org/10.1002/jor.1100070415
  9. Ohgushi H, Dohi Y, Yoshikawa T, Inoue K. et al : Osteogenic differentiation of cultured marrow stromal stem cells on the surface of bioactive glass ceramics. J Biomed Mat Res 32 : 341, 1996 https://doi.org/10.1002/(SICI)1097-4636(199611)32:3<341::AID-JBM6>3.0.CO;2-S
  10. Ohgushi H, Okumura M, Yoshikawa T, Inoue K. et al : Droped apatite-wollaston-ite containing glass ceramic provokes osteogenic differentiation of marrow stromal stem cells. J Biomed Mater Res 44 : 381, 1999 https://doi.org/10.1002/(SICI)1097-4636(19990315)44:4<381::AID-JBM3>3.0.CO;2-E
  11. Tsutsumi S, Shimazu A, Miyazaki K, Pan H. et al : Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochemical and Biophysical Research Communications 288 : 413, 2001 https://doi.org/10.1006/bbrc.2001.5777
  12. Ueda M, Yamada Y, Naiki T, Takahachi M. et al : Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma.- Tissue engineered bone regeneration. Tissue Engineering 10 : 955, 2004 https://doi.org/10.1089/1076327041348284
  13. Ohgushi H, Dohi Y, Katuda T, Tamai S. et al : In vitro bone formation by rat marrow cell culture. J Biomed Mat Res 32 : 333, 1996 https://doi.org/10.1002/(SICI)1097-4636(199611)32:3<333::AID-JBM5>3.0.CO;2-T
  14. Kitamura S, Hirose M, Funaoka H, Takakura Y. et al : Osteogenic differentiation of human bone marrow derived mesenchymal cells cultured on alumina ceramics. Artif Organs 28 : 72, 2003
  15. Ohgushi H, Okumura M, Yoshikawa T, Inoue K. et al : Bone formation process in porous calcium carbonate and hydroxyapatite. J Biomed Mat Res 26 : 885, 1992 https://doi.org/10.1002/jbm.820260705
  16. Hasegawa Y, Ishimura M, Habata T, Tamai S. et al : Marrow cell culture on poly-L-lactic acid fabrics. Clin Orthop 358 : 235, 1999
  17. Harris CT, Cooper LF : Comparison of bone graft matrices for human mesenchymal stem cell-directed osteogenesis. J Dent Res 25 : 747, 2004
  18. Martin RB : Skeletal biology. In : 29, 1998
  19. Bruder SP, Fox BS : Tissue engineering of bone: Cell based strategies. Clin Orthop 367 : 68, 1999 https://doi.org/10.1097/00003086-199910001-00008
  20. Liechtye KW, Mackenzie TC, Shaaban AF : In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res 238 : 265, 2000
  21. Marx RE, Carson ER, Eichstaedt RM. et al : Platelet-rich plasma, growth factor enhancement for bone grafts. Oral Surg. Oral Med. Oral Patho 85 : 638, 1998 https://doi.org/10.1016/S1079-2104(98)90029-4
  22. Anitua E : Plasma rich in growth factors: preliminary results of use in the treatment of future sites for implants. Int J Oral Maxillofac Implants 14 : 529, 1999
  23. Noshi T, Gibran NS, Isik FF, Heimbach DM. et al : Recombinant human bone morphogenic protein-2 potentiates the in vivo osteogenic ability of marrow/hydroxyapatite composites. Artificial Organs 25 : 201, 2001 https://doi.org/10.1046/j.1525-1594.2001.025003201.x
  24. Rose LF, Rosenberg E : Bone grafts and growth and differentiation factor for regenerative therapy. Aesthet Dent 13 : 725, 2001
  25. Cochran DL, Woxney JM : Biological mediators for periodontal regenera tion. Periodontol 19 : 19, 2000
  26. Kadiyara S, Young RG, Thiede MA, Bruder SP. et al : Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplantation 6 : 125, 1997b https://doi.org/10.1016/S0963-6897(96)00279-5
  27. Younger EM, Chapman MW : Morbidity at bone graft donor sites. J Orthop Trau ma 3 : 192, 1989 https://doi.org/10.1097/00005131-198909000-00002
  28. Lundgren S, Moy P, Johansson C, Nilsson H : Augmentation of the maxillary sinus floor with particulated mandible. Int J Oral Maxillofac Implants 11 : 760, 1996
  29. Piatelli M, Favero GA, Scarano A, Orsini G, Piatelli A : Bone reactions to anorganic bovine bone (Bio-Oss) used in sinus augmentation procedures. Int J Oral Maxillofac Implants 14 : 835, 1999
  30. Jensen OT, Sennerby L : Tissue reaction and material characteristics of four bone substitutes. Int J Oral Maxillofac Implants 11 : 55, 1996
  31. Buser D, Dula K, Hess D, Hirt HP : Lateral ridge augmentation using different bone fillers and barrier membrane application. Clin Oral Impl Res 12 : 260, 2001 https://doi.org/10.1034/j.1600-0501.2001.012003260.x
  32. Cho MI, Lee DM : Platelet derived growth factor modulated guided tissue regenerative therapy. J Periodont 66 : 522, 1995 https://doi.org/10.1902/jop.1995.66.6.522