This work was to determine the microbial and physico-chemical changes of HTST-pasteurized soymilk using microwave energy. Soymilk was HTST pasteurized$(at\;90^{\circ}C\;for\;20\;sec)$ by three methods: by heating in a stainless steel tube immersed in a hot water bath(MP0), by heating in a microwave cavity to a defiled temperature and then holding in a hot water bath(MP1), and by both heating and holding in a microwave cavity(MP2). The microbial quality based on the total plate count was in the order of MP0, MP2 and MP1. The three samples pasteurized by different methods showed the similar microbial quality with respect to the coliform count, psychrotrophic bacterial count and phosphatase activity. The destruction of trypsin inhibitor was in the order of MP0, MP1 and MP2. There were no significant differences in pH, titratable acidity, viscosity and vitamin $B_2$ content before and after pasteurization and among the different pasteurization methods. The similar or higher quality retention of the MP1 or MP2 supports the possibility of using microwave energy for the HTST pasteurization of soymilk and other fluid food products.
A dielectric barrier discharge plasma (DBDP) treatment system was fabricated and the optimum operating conditions for the plasma generation were determined in order to explore the potential of cold plasma as a non-thermal proessing technology. The microbial inactivation performance of the system was also evaluated against Staphyloocus aureus. The system consisted of power supply, transformer, electrode assembly and sample treatment plate. The input power was 220 V single phase AC and amplified to 10.0-50.0 kV on a transformer. A pulsed sine wave of frequency 10.0-50.0 kHz was introduced to the electrode embedded in ceramic as a dielectric barrier material in order to generate plasma at atmospheric pressure. Higher currents and consequently greater power were required for the plasma generation as the frequencies increased. A homogeneous and stable plasma was generated at currents of 1.0-2.0, and frequencies of 32.0-35.3 kHz. The optimum electrode-gaps for the plasma generation were 1.85 mm without loaded samples. More power was consumed as the electrode-gaps increased. The practically optimum electrode- gap was, however, 2.65 mm when samples were treated on slide-glasses for microbial inactivation. The maximum temperature increase after 10 min treatment was less than 20$^{\circ}C$, indicating no microbial inactivation effect by heat and thereby insuring a non-thermal method. The DBDP inactivation effect against Staphyloocus aureus increased linearly with treatment time up to 5 min, but plateaued afterward. More than 5 log reduction was achieved by 10 min treatment at 1.25 A.
Lee Yong Ha;Park Kyung Ran;Lee Jong Young;Lee Ik Jae;Park Young Woo;Lee Kang Kyoo
Radiation Oncology Journal
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v.21
no.4
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pp.322-329
/
2003
Purpose: It is difficult to exactly determine the surface dose and the dose distribution In buildup region of high energy X-rays by using the conventional ion chamber. The aim of this study Is to evaluate the accuracy of widely used dosimetry systems to measure the surface dose and the depth of maximum dose (d$_{max}$). Materials and Methods: We measured the percent depth dose (PDD) from the surface to the d$_{max}$ in either a water phantom or in a solid water phantom using TLD-100 chips, thimble type ion chamber, diode detector, diamond detector and Markus parallel plate ion chamber for 6 MV and 15 MV X-rays, 10$\times$10 cm$^{2}$, at SSD=100cm. We analysed the surface dose and the d$_{max}$. In order to verify the accuracy of the TLD data, we executed the Monte Carlo simulation for 5 MV X-ray beams. Results: The surface doses In 6 MV and IS MV X-rays were 29.31% and 23.36% ior Markus parallel plate ion chamber, 37.17$\%$ and 24.01$\%$ for TLD, 34.87$\%$ and 24.06$\%$ for diamond detector, 38.13$\%$ and 27.8$\%$ for diode detector, and 47.92$\%$ and 35.01$\%$ for thimble type ion chamber, respectively. in Monte Carlo simulation for 6 MV X-rays, the surface dose was 36.22$\%$, which Is similar to the 37.17$\%$ of the TLD measurement data. The d$_{max}$ In 6 WV and 15 MV X-rays was 14$\~$16 mm and 27$\~$29 mm, respectively. There was no significant difference in the d$_{max}$ among the detectors. Conclusion: There was a remarkable difference in the surface dose among the detectors. The Markus parallel plate chamber showed the most accurate result. The surface dose of the thimble ion chamber was 10$\%$ higher than that of other detectors. We suggest that the correction should be made when the surface dose of the thimble ion chamber Is used for the treatment planning ion the supeficial tumors. All the detectors used In our study showed no difference in the d$_{max}$.
Purpose: The aim of this study was to investigate the effect of implant thread profile on the marginal bone stresses which develop during implant insertion. Materials and methods: Four experimental implants were created by placing four different thread systems on the body ($4.1mm{\times}10mm$) of the ITI standard implant. The thread types studied in this study included the buttress, v-shape, reverse buttress, and square shape threads. In order to examine the insertion stress generation, 3D dynamic finite element analysis was performed which simulated the insertion process of implants into a 1.2 mm thick cortical bone plate (containing 3.5 mm pilot hole) using a PC-based DEFORM 3D (ver 6.1, SFTC, Columbus, OH, USA) program. Results: Insertion stresses higher than human cortical bone developed around the implants. The level of insertion stresses was much different depending on the thread. Stress level was lowest near the v-shape thread, and highest near the square shaped thread. Difference in the interfacial bone stress level was more noticeable near the valley than the tip of the threads. Conclusion: Among the four threads, the v-shape thread was turned out to minimize the insertion stress level and thereby create better conditions for implant osseointegration.
This study was to evaluate the biological activity of flavor components extracted from E. ciliata and E. splendens in order to survey the possibility applicable to tobacco and food industry. Flavor components were extracted with dividing into three parts; essential oil, absolute, oleoresin. In the nonenzymatic lipid peroxidation system, the inhibition rate($\%$) of essential oil were $67.3\;\pm\;20.7\%,\;58.1\;\pm\;19.3\%$ at the concentration of 50 ${\mu}g/mL$ of E. ciliata and E. splendens, respectively. The inhibition rate($\%$) of the oleoresin in E. ciliata was higher than one in E. splendens. In the enzymatic lipid peroxidation system, the inhibition rate($\%$) of essential oil and oleoresin was$14.28\;\pm\;2.38\%,\;and\;65.93\;\pm\;0.01\%,\;and\;was\;22.58\;\pm\;2.84\%\;and\;40.73\;pm\;6.04\%$. The oleoresin of two species were showed above $90\%$ of the inhibition rate($90\%$) against autooxidative lipid peroxidation system. $EC_{50}$ values in neutral red uptake assays 24 h of exposure times were $23.3\;{\mu}g/mL,\;341.0\;{\mu}g/mL\;and\;17.2\;{\mu}g/mL$ in essential oil, absolute and oleoresin from E. ciliata respectively, and were $46.4\;{\mu}g/mL,\;681.7\;{\mu}g/mL\;17.6\;{\mu}g/mL$ in three extractions of E. splendens. Oleoresin of two species showed high rate in the cytotoxic effect by neutral red uptake assay. Absolute and oleoresin did not show antibiotic and mutagenic activity. On the contrary, essential oil with over 500 ug/plate showed antibiotic and mutagenic activity in Ames test. Essential oil and oleoresin have a prolongating effect the ciliostasis of rat trachea. This results indicate that flavor components extracted from E. ciliata and E. splendens can be considered to be toxicological safe and to be the possibility applicable the cigarette, food and drug industry as a flavor for expectoration.
KSCE Journal of Civil and Environmental Engineering Research
/
v.32
no.1A
/
pp.11-18
/
2012
This paper deals with the numerical determination of the stress intensity factors of cracked aluminum plates under the mixed mode of $K_I$ and $K_{II}$ in glass-epoxy fiber reinforced composites. For the stress intensity factors, two different models are reviewed such as VCCT and two-step extension method. The p-convergent partial layerwise model is adopted to determine the fracture parameters in terms of energy release rates and stress intensity factors. The p-convergent approach is based on the concept of subparametric element. In assumed displacement field, strain-displacement relations and 3-D constitutive equations of a layer are obtained by combination of 2-D and 1-D higher-order shape functions. In the elements, Lobatto shape functions and Gauss-Lobatto technique are employed to interpolate displacement fields and to implement numerical quadrature. Using the models and techniques considered, effects of composite laminate configuration according to inclined angles and adhesive properties on the performance of bonded composite patch are investigated. In addition to these, the out-of-plane bending effect has been investigated across the thickness of patch repaired laminate plates due to the change of neutral axis. The present model provides accuracy and simplicity in terms of stress intensity factors, stress distribution, number of degrees of freedom, and energy release rates as compared with previous works in literatures.
Interleukin (IL)-32 is a recently identified proinflammatory cytokine that is one of the IL-18 inducible genes, and plays an important role in autoimmune and inflammatory diseases. We produced antibodies against IL-32 and studied the expression of IL-32 in human stomach cancer. We detected IL-32 secreted from K-562 cells which were stably transfected with IL-32 and in the sera of stomach cancer patients by a sandwich ELISA using a monoclonal antibody KU32-52 and a polyclonal antibody. In order to optimize a sandwich immunoassay, recombinant IL-32a was added, followed by the addition of a biotinylated KU32-52 into microtiter plate wells precoated with a goat anti-IL-32 antibody. The bound biotinylated KU32-52 was probed with a streptavidin conjugated to HRP. This sandwich ELISA was highly specific and had a minimal detection limit of 80 pg/ml (mean${\pm}$SD of zero calibrator) and measuring up to 3,000 pg/ml. This ELISA showed no cross-reaction with other cytokines such as hIL-1$\alpha$, hIL-1$\beta$, hIL-2, hIL-6, hIL-8, hIL-10, hIL-18, and hTNF-$\alpha$. Intra-assay coefficients of variation were 18.5% to 4.6% (n=10), and inter-assay coefficients were 23% to 9% (n=10). The average IL-32 level in the sera of 16 stomach cancer patients (189 pg/ml) was higher than that of 12 healthy control men (109 pg/ml). Our results indicate that serum IL-32 level can be detected by using an established ELISA, and that this immunoassay and mAb KU32-09 specific for immunohistochemistry can be used in the detection of expressed and secreted IL-32 in stomach cancer patients.
Lee, Cheol Ho;Kim, Dae Kyung;Han, Kyu Hong;Park, Chang Hee;Kim, Jin Ho;Lee, Seung Eun;Kim, Do Hwan
Journal of Korean Society of Steel Construction
/
v.26
no.5
/
pp.407-418
/
2014
As a continuing work of previously conducted standard tension tests, full-scale flexural tests were conducted in this study to assess the structural performance the CJP groove welded joints connecting thick HSA800 plates. Two welding electrodes were available at the time of this experimental research; one was GMAW-based electrode A and the other FCAW-based electrode B. Three full-scale box-type beam specimens with single bevel- and V-groove CJP welded joints were fabricated from 60mm and 25mm thick HSA800 plates according to the AWS-prequalified groove welded joint details. In designing the specimens, all possible limit states like local and lateral torsional buckling were carefully controlled in order to induce flexural plastic yielding or eventual joint fracture. All the CJP joints made by both welding electrodes showed satisfactory performance and were able to transfer the tensile flange forces higher than that corresponding to the measured tensile strength of HSA800 flange plates. However, it should be noted that, during fabrication, serious concerns about the welding efficiency and workability of the GMAW-based electrode were raised by a certified welder. The fracture occurred at the unbeveled (or vertical) interface between the weldment and the base metal when the GMAW-based electrode was used in the single-bevel joint, implying the possibility of insufficient melting. Thus, the FCAW-based electrode B is again recommended as the choice of welding electrode for HSA800 plates. The limited test data of this study implies that the V-groove CJP joint should be used in favor of the single bevel CJP joint, if possible.
Journal of the Society of Naval Architects of Korea
/
v.54
no.5
/
pp.406-414
/
2017
An experimental study in a recirculating water channel was carried out to investigate the effect of large coherent structures to the skin friction on a flat plate. Particle Image Velocimetry (PIV) technique was used to quantify characteristic features of coherent structures growing to the boundary layer. In the PIV measurement, it is difficult to calculate the friction velocity near the wall region due to laser deflection and uncertainty so that Clauser fitting method at the logarithmic region was adopted to compute the friction velocity and compared with the one directly measured by the dynamometer. With changing the free-stream velocity from 0.5 m/s to 1.0 m/s, the activity of coherent structures in the logarithmic region was increased over three times in terms of Reynolds stress. The flow field was separated by Variable Interval Time Averaging (VITA) technique into the weak and the strong structure case depending on the existence large coherent structures in order to validate its effectiveness. The stream-wise velocity fluctuation was scanned through at the boundary thickness whether it had a large deviation from background flow. With coherent structures connected from near-wall to the boundary layer, mean wall shear stress was higher than that of weak structure case. Proper Orthogonal Decomposition (POD) analysis was also applied to compare the energy budget between them at each free-stream velocity.
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
/
v.3
no.3
/
pp.149-157
/
1998
Seasonal changes of topography, sediment grain size and accumulation rate in the Gomso-Bay tidal flat, west coast of Korea, have been studied in order to understand the seasonal accumulation pattern and preservation potential of the tidal-flat sediments. Seasonal levelings across the tidal flat show that the landward movement of both intertidal sand shoals and cheniers accelerates during the winter and typhoon periods, but it almost stops in summer when mud deposition is instead predominant at the middle and upper tidal flats. Seasonal variations of mean grain size were largest on the upper part of middle tidal flat where summer mud layers were eroded during the winter and typhoon periods. Measurements of accumulation depths from sea floor to basal plate reveal that accumulation rates were seasonally controlled according to the elevation of tidal-flat surface. The upper tidal flat where the accumulation rate of summer was generally higher than that of winter was characterized by a continuous deposition throughout the entire year, whereas in the middle tidal flat, sediment accumulations were concentrated in winter relative to summer and were intermittently eroded by typhoons. The lower tidal flat were deposited mostly in winter and eroded during summer typhoons. Can cores taken across the tidal flat reveal that sand-mud interlayers resulting from such seasonal changes of energy regime are preserved only in the upper part of the deposits and generally replaced by storm layers downcore. Based on above results, it is suggested that the storm deposits by winter storms and typhoons would consist of the major part of the Gomso-Bay sediments.
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