• Journal of Microbiology and Biotechnology
• /
• 제15권3호
• /
• pp.551-559
• /
• 2005

The recent rapid increase in genomic data related to many microorganisms and the development of computational tools to accurately analyze large amounts of data have enabled us to design several kinds of simulation approaches for the complex behaviors of cells. Among these approaches, dFBA (dynamic flux balance analysis), which utilizes FBA, differential equations, and regulatory events, has correctly predicted cellular behaviors under given environmental conditions. However, until now, dFBA has centered on substrate concentration, cell growth, and gene on/off, but a detailed hierarchical structure of a regulatory network has not been taken into account. The use of Boolean rules for regulatory events in dFBA has limited the representation of interactions between specific regulatory proteins and genes and the whole transcriptional regulation mechanism with environmental change. In this paper, we adopted the operon as the basic structure, constructed a hierarchical structure for a regulatory network with defined fundamental symbols, and introduced a weight between symbols in order to solve the above problems. Finally, the total control mechanism of regulatory elements (operons, genes, effectors, etc.) with time was simulated through the linkage of dFBA with regulatory network modeling. The lac operon, trp operon, and tna operon in the central metabolic network of E. coli were chosen as the basic models for control patterns. The suggested modeling method in this study can be adopted as a basic framework to describe other transcriptional regulations, and provide biologists and engineers with useful information on transcriptional regulation mechanisms under extracellular environmental change.

• 청정기술
• /
• 제28권2호
• /
• pp.97-102
• /
• 2022

Lithium sulfur (Li-S) batteries have attracted considerable attention as a promising candidate for next-generation power sources due to their high theoretical energy density, low cost, and eco-friendliness. However, the poor electrical conductivity of sulfur and its insoluble discharging products (Li₂S₂/Li₂S), large volume changes, severe self-discharge, and dissolution of lithium polysulfide intermediates result in rapid capacity fading, low Coulombic efficiency, and safety risks, hindering Li-S battery commercial development. In this study, a three-dimensionally (3D) connected hierarchical porous carbon framework (HPCF) derived from waste sunflower seed shells was synthesized as a sulfur host for Li-S batteries via a chemical activation method. The natural 3D connected structure of the HPCF, originating from the raw material, can effectively enhance the conductivity and accessibility of the electrolyte, accelerating the Li⁺/electron transfer. Additionally, the generated micropores of the HPCF, originated from the chemical activation process, can prevent polysulfide dissolution due to the limited space, thereby improving the electrochemical performance and cycling stability. The HPCF/S cell shows a superior capacity retention of 540 mA h g^-1 after 70 cycles at 0.1 C, and an excellent cycling stability at 2 C for 700 cycles. This study provides a potential biomass-derived material for low-cost long-life Li-S batteries.

• 대한전자공학회논문지SD
• /
• 제46권9호
• /
• pp.14-24
• /
• 2009

System on panel 프레임 버퍼를 위한 메모리 셀 어레이와 주변회로가 설계되었다. 또한, system on panel 공정의 낮은 yield를 극복하기 위해, 블럭 단위의 parallel test 방안이 제안되었다. 기존의 메모리 테스트 보다 빠르게 fault detection이 가능하며, 다양한 embedded memory나 일반 SRAM 테스트 분야에도 적용 가능하다. 또한 기존의 다양한 test vector pattern이 그대로 적용될 수 있어 fault coverage가 높고, 최근의 추세인 hierarchical bit line과 divided word line 구조에도 적용될 수 있다.

• Plant Biotechnology Reports
• /
• 제3권1호
• /
• pp.87-93
• /
• 2009

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate cultivars metabolically, leaves and fruits of five commercial strawberry cultivars were subjected to Fourier transform infrared (FT-IR) spectroscopy. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Fisher's linear discriminant function analysis. The dendrogram based on hierarchical clustering analysis of these spectral data separated the five commercial cultivars into two major groups with originality. The first group consisted of Korean cultivars including 'Maehyang', 'Seolhyang', and 'Gumhyang', whereas in the second group, 'Ryukbo' clustered with 'Janghee', both Japanese cultivars. The results from analysis of fruits were the same as of leaves. We therefore conclude that the hierarchical dendrogram based on PCA of FT-IR data from leaves represents the most probable chemotaxonomical relationship between cultivars, enabling discrimination of cultivars in a rapid and simple manner.

• Journal of Plant Biotechnology
• /
• 제34권3호
• /
• pp.201-205
• /
• 2007

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts of higher plants is applied to discriminate plants genetically, leaf samples of eight cultivars of Catharanthus roseus were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR fingerprint region data were analyzed by principal component analysis (PCA). Major peaks as biomarkers were identified as the most significant contributors to distinguish samples by using genetic programming. A hierarchical dendrogram based on the results from PCA separated the eight cultivars into two major groups in the same manner as the dendrograms based on genetic fingerprinting methods such as RAPD and AFLP. A slight difference between the dendrograms was found only in branching pattern within each subgroup. Therefore, we conclude that the hierarchical dendrogram based on PCA of the FT-IR data represents the most probable chemotaxonomical relationship between cultivars, which is in general agreement with the genetic relationship determined by conventional DNA fingerprinting methods.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2003년도 학술회의 논문집 정보 및 제어부문 A
• /
• pp.163-166
• /
• 2003

In recent years, there has been increased to access multimedia service through the Internet due to rise of the access speed. We suppose the co-existence of the various access networks in adjacent area in the future, such as wireless LAN, Bluetooth, and 3G Cellular So, the size of the cell will be smaller and the number of access point will be increased. In other words, the potable devices need the efficient mobility scheme due to move between cells frequently Currently, the several of the scheme, which is Cellular IP, HAWAII, Mobile IPv4 regional registration, and hierarchical Mobile IPv6, is suggested to support micro-mobility but Mobile IPv4 is standard scheme to support the mobility of the mobile node. MPLS, which forwards the packet through switching instead of routing using IP address, has begun to deploy in the internet backbone to increase the performance of mobility protocol. The integration of both MPLS and Mobile IP improves the scalability of the Mobile IP data forwarding process by leveraging on the features MPLS which are fast switching and high scalability. Moreover it abbreviates IP-in-IP tunneling between HA and FA. In this paper, we suggest efficient mobility scheme through integration of both MPLS and hierarchical Mobile IPv6.

• Communications for Statistical Applications and Methods
• /
• 제29권5호
• /
• pp.547-559
• /
• 2022

For a chi-squared test, which is a statistical method used to test the independence of a contingency table of two factors, the expected frequency of each cell must be greater than 5. The percentage of cells with an expected frequency below 5 must be less than 20% of all cells. However, there are many cases in which the regional expected frequency is below 5 in general small area studies. Even in large-scale surveys, it is difficult to forecast the expected frequency to be greater than 5 when there is small area estimation with subgroup analysis. Another statistical method to test independence is to use the Bayes factor, but since there is a high ratio of data dependency due to the nature of the Bayesian approach, the low expected frequency tends to decrease the precision of the test results. To overcome these limitations, we will borrow information from areas with similar characteristics and pool the data statistically to propose a pooled Bayes test of independence in target areas. Jo et al. (2021) suggested hierarchical Bayesian pooling models for small area estimation of categorical data, and we will introduce the pooled Bayes factors calculated by expanding their restricted pooling model. We applied the pooled Bayes factors using bone mineral density and body mass index data from the Third National Health and Nutrition Examination Survey conducted in the United States and compared them with chi-squared tests often used in tests of independence.

• 한국정보통신학회논문지
• /
• 제13권1호
• /
• pp.67-74
• /
• 2009

본 논문에서는 이동통신 네트워크의 계층 셀 구조에서 음성호와 데이터호의 트래픽을 분산시킴으로서 서비스의 이용성을 개선시키는 방안을 제안한다. 계층 셀 구조의 트래픽 모델을 기술하고, 호 처리에 대한 성능 분석이 수행된다. 본 논문에서 제안된 호 처리 방식에서, 빠른 속도로 이동하는 호는 상위 계층 셀로 이동하여 서비스된다. 오버플로우가 발생하는 경 우에도 호는 상위 계층으로 이동한다. 다른 방식들과 달리, 오버플로우가 발생되어 상위 계층으로 이동한 호는 하위 계층에서 가장 오래 동안 서비스된 호이다. 오버플로우가 발생하여 상위 계층 셀로 이동된 호가 일정시간 이상상위 계층에서 서비스되면, 상위 계층 셀의 한정된 자원을 절약하기 위해 하위 계층으로 호를 이동시킨다. 제안된 방식의 호 처리 성능분석을 위해 M/M/C/K모델을 이용하여 평가하고, 호 블록킹 확률과 강제종료 확률이 개선됨을 보여준다.

• International Journal of Oral Biology
• /
• 제34권2호
• /
• pp.115-121
• /
• 2009

Bone morphogenetic protein (BMP) 2 is a potent osteogenic factor. Although both Smad1/5 and mitogenactivated protein kinases (MAPKs) are activated by BMP2, the hierarchical relationship between them is unclear. In this study, we examined if BMP2-stimulated MAPK activation is regulated by Smad1/5 or vice versa. When C2C12 cells were treated with BMP2, the activation of extracellular signal-regulated kinase (ERK), p38 MAPK and c-Jun-N-terminal kinase was evident within 5 min. The knockdown of both Smad1 and Smad5 by small interfering RNA did not affect the activation of these MAPKs. In addition, neither the overexpression of Smad1 nor Smad5 induced ERK activation. When ERK activation was induced by constitutively active MEK1 expression, the protein level and activation of Smad1 increased. Furthermore, the inhibition of constitutively active BMP receptor type IB-induced ERK activation significantly suppressed Smad1 activation. These results indicate that Smad1/5 activation is not necessary for BMP2-induced MAPK activation and also that ERK positively regulates Smad1 activation.

• 동의생리병리학회지
• /
• 제20권4호
• /
• pp.853-860
• /
• 2006

This study was performed to investigate genes which are differently expressed in human blood by administrating of OLT-2. OLT-2 was medical precipitation composed of three medicinal herbs, Ginseng Radix, Astragali Radix, Glycyrrhizae Radix, and anti-leukemia effect of it was evaluated from Byung Hun Jeon of Wonkwang University this study was approved by Institutional Review Board of Korea Institute of Oriental Medicine (Taejeon, Korea) and four male subjects participated in this study. Gene expressions were evaluated by cDNA chip, in which 24,000 genes were spotted. Hierarchical cluster and biological process against the genes, which expression changes were more than 1.6 fold, were constructed by cluster 3.0 providing Stanford University and EASE(http://apps1 .maid.nih.gov/DAVID). Five groups were clustered according to their expression patterns. Group A contained gene decreased by OLT-2 and increased genes by OLT-2 were involved in Group B, C, D. In biological process, expression of genes involved in cytokine or cell calcium signaling, such as interleukin 18 and G-protein beta 4 were increased, but protein tyrosine phosphatase receptor type c, which function is cell adhesion between antigen-presenting cell and T or B-cell, was decreased by OLT-2. This study provides the most comprehensive available survey of gene expression changes in response to anti-leukemia effect of OLT-2 in human blood.