• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.2
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• pp.397-402
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• 2008

The purpose of this study is to prepare black Panax ginseng leaf (PGL) and evaluate its antioxidative effect. In order to make black PGL, the raw PGL was successiely steamed at $95^{\circ}C$ for 3 hr nine times. The antioxidant activities of total saponins (Sa) from PGL and black PGL against peroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. Specific TOSC values for black PGL-Sa against peroxyl radicals and peroxynitrites were 2.3-fold and 2.1-fold of PGL-Sa, respectively, and 2.2-fold and 5.2-fold of glutathione, a positive control antioxidant, respectively. The black PGL-Sa exhibited stronger antioxidative effect than PGL-Sa. The main ginsenosides of black PGL were $Rg_3,\;Rk_1\;and\;Rg_5$. Among the saponins in black PGL, the amount of ginsenoside $Rg_3$ was examined by HPLC. 22.12 mg of ginsenoside $Rg_3$ was obtained from 1g of dried black PGL.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.5
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• pp.888-892
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• 2002

Nae-Kyung says the puberty is corresponded to the age of 16～24(male) and 14～21 (female). And that time they are promoted the growth. However, Kidney qi doesn't make average situation during that time. So, the function of five viscera and six entrails fails to be perfect and symptoms appear. I came to the conclusion through the survey of about 200 high school students(male and female) and the theory of oriental medicine the distinctive features (like migraine, amnesia, depression, dysphoria, inappetence, irregular menstruation) came out that time. I defined this distinctive symptoms in boys and girls at puberty ‘synthetic symptoms of puberty’. For the medical cure and prevention I prescribed ‘Sachun-Nyung’ and that has medical benefits for ‘Replenishing qi and relieving the spleen, Soothing the liver and promoting blood circulation’

• Journal of Korean Traditional Oncology
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• v.14 no.1
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• pp.75-84
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• 2009

Gekko has been used for several diseases including cancer in Oriental medicine and fork herbalogy. Nevertheless, its origin as herbal medicine and its efficacy and mechanism as anti-tumor drug have not yet been thoroughly reported in Korea. This study aimed to investigate anti-tumor effect of Gekko through selected articles from cqvip database in China. In vitro and In vivo, Gekko could obviously inhibit tumor growth, induce tumor cells apoptosis, reduce micro-vessel density in tumor tissue through down regulating VEGF & bFGF protein expression, promote cytotoxicity of lymphocyte. Gekko could improve survival rate, relive clinical symptoms, improve quality of life, and relieve anti-tumor treatment reaction, suggesting that Gekko might be a effective anti-tumor drug.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.1
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• pp.115-121
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• 2011

This study was performed to investigate the antioxidant activity of extracts of black Panax ginseng (BGE) and its crude saponin (BGECS). The antioxidant activities of BGE and BGECS were evaluated for free radical scavenging activity against stable free radical (1,1-diphenyl-2-picrylhydrazyl) DPPH, nitrite, hydrogen peroxide and superoxide. In addition, the antioxidant activity of BGE and BGECS against peroxyl radicals, hydroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. As a result, BGE and BGECS were found to have a strong inhibitory activity with >90% against the DPPH radical at $1000{\mu}g/m{\ell}$ concentrations. Also, BGE and BGECS exhibited strong inhibitory activity with >80% against hydrogen peroxide at lower concentration ($125{\mu}g/m{\ell}$). Moreover, specific TOSC values (405 and 473 TOSC/mM) of BGE and BGECS against peroxynitrites were higher than GSH (347 TOSC/mM) used a positive control. These results suggest that BGE and BGECS could be useful to develop functional foods against disease related oxidative stress.

• The Korea Journal of Herbology
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• v.23 no.1
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• pp.45-51
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• 2008

Objectives : For the purpose of developing Korean herbalogy of the plants belonging to Polypodiaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. The results of this study are as follows : Methods : The examined herbalogical books and research papers which published at home and abroad. Results : 1. There are totaled to 8 genera and 24 species in Polypodiaceae in Korea and among them medicinal plants are 7 genera, 11 species, some 46% in total. 2. Pyrrosia genus is main kind enough that it has 5 species among 24 species in Polypodiaceae, of which medicinal plants are 3 species. 3. The herb is the main medicinal parts if medicinal plants in the Polypodiaceae, which is used in 9 species. 4. According to nature and flavor of medicinal plants in the Polypodiaceae, they were classified into cool 9 species, and cold 5; sweet taste 10 and bitter taste 8 in the order. 5. According to meridian propism of medicinal plants in the Polypodiaceae, they were classified into bladder meridian 5 species, lung meridian 4 species. 7.The number of toxic species in the Polypodiaceae was examined to be none. Conclusions : There are totaled to 8 genera and 24 species in Polypodiaceae in Korea and among them medicinal plants are 7 genera, 11 species, some 46% in total.

• The Korea Journal of Herbology
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• v.23 no.1
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• pp.39-44
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• 2008

Objectives : Through surveying the vegetation of woody plants naturally growing in the Chonnam Area. we expect to make good use of the surveyed data for the conservation and utilization of woody plants in the Chonnam Area. And also we hope that we could make a good opportunity to love and conserve the Chonnam Area through this report. Methods : we inspected native and cultivated medicinal woody plants in the Chonnam Area by report and survey, and researched medicinal part through the herbalogy literature. Results : 1. According to the investigation of medicinal woody plant in the Chonnam area, it bears 189 species, 139 genera and 62 families. 2. Rosaceae and Quercus are the biggest share of medicinal woody plants in the Chonnam area. 3. In aspect of medicinal part, roots of 100 species, leaves of 102 species, fruits of 80 species, and barks of 67 species have been using. The leaves part of the most plants are abundantly used. 4. About 30 species of medicinal woody plants are distributed distinctively in Korea. Conclusions : According to the investigation of medicinal woody plant in the Chonnam area, it bears 189 species, 139 genera and 62 families.

• The Korea Journal of Herbology
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• v.27 no.5
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• pp.77-83
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• 2012

Objective : For the purpose of developing Korean herbalogy of the plants to Violaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods : The examined herbalogical books and research paper which published at home and abroad. Results : 1. Violaceae plants (raised in Korea) are devided into 1 classes with 57 species. Among them, 14 species in 1 classes were found serviceable, which indicates 32 % of all Violaceae plants. 2. The herb is main medicinal part if medicinal plants in the Violaceae, which is used in 14 species. 3. According to the nature and flavor of medicinal plants in the Violaceae, they were classified into cold 8 species, and cool 7; bitter taste 8; acrid taste and little bitter taste 6 in the order. 4. According to the meridian propism of medicinal plants in the Violaceae, they were classified into liver meridian 6 species, heart meridian 3 species in the order. 5. According to the properties and principal curative action, they were classified into drugs for clearing heat, removing toxins 12 species; drugs for alleviate edema 7, and drugs for arresting bleeding 6 in the order. 6. There were no toxic species in the Violaceae family. Conclusion : There were totaled to 1 genera and 57 species in Violaceae in Korea and among them medicinal plants are 1genera, 14 species, some 32% in total.

• The Korea Journal of Herbology
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• v.26 no.4
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• pp.59-65
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• 2011

Objective : For the purpose of developing Korean herbalogy of the plants to Aspleniaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods : The examined herbalogical books and research paper which published at home and abroad. Results : 1. There are 2 genera and 21 species in Aspleniaceae in Korea and among them medicinal plants are 2 genera and 10 species, some 48% in total. 2. Asplenium genus is a main kind in that it has 20 species among 21 species in Aspleniaceae of which medicinal plants are 9 species. 3. The Herb play medicinal parts if most plants in the Aspleniaceae have the effect of a medicine, the 10 species of which are used for medical care. 4. According to nature and flavor of medicinal plants in the Aspleniaceae, the cold medicinal plants and the bitter medicinal plants take the highest number of them 5. According to the properties and principal curative action, they were classified into drugs for hemostatic 10 species, drugs for clearing away heat 8 species, drugs for detoxification 7 species respectively. 6. None of the species of Aspleniaceae have been found to be toxic. Conclusions : There are totaled to 2 genera and 21 species in Aspleniaceae in Korea and among them medicinal plants are 2 genera and 10 species some 48% in total.

• The Journal of Korean Medicine
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• v.19 no.2
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• pp.326-336
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• 1998

면역 결핍 바이러스와 허피즈 바이러스 -1,2에 대한 한약의 항바이러스 작용을 관찰하기 위하여, 세포 바이러스 검색에 초기 독성과 감염 바이러스의 생존 세포 수를 비교적 단기간내 볼 수 있는 96-well plate를 이용한 MTT assay로 측정하였다. 본 실험은 예비 실험 단계에서 사용된 총 85가지의 한약중 단미제 6가지와 복합처방 44가지를 선정하였으며 복합처방은 유사한 치법으로 분류하였고 한약의 시료 추출은 순수하게 물로 전탕하여 여과하였다. 실험 결과 파두와 맥아가 면액 결핍 바이러스에 대해 감염초기 유의성이 있었으며 호장근, 갈근우방자탕과 형방패독산이 허피즈 바이러스-1,2에 대해 감염초기 유의성이 있었으나 실험의 시간 경과에 따른 지속적인 약효 안정성을 보이지는 못하였다. 이 실험을 통하여 한약을 이용한 우수한 바이러스 치료를 개발하기 위해서는 단미제나 복합 처방에서 항바이러스 작용이 큰 유효성분의 대량 분리와 세포 실험에 있어서 오차를 줄 일 수 있는 세포면역학적 실험의 도입 등이 필요할 것으로 사료된다.

• Korean Journal of Oriental Medicine
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• v.15 no.3
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• pp.99-105
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• 2009

In this research, the genotoxic effects of Pinelliae Rhizoma (PR) extracts, one of famous herbal agents in Korean medicine were evaluated using the mouse micronucleus test. PR extracts was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000, and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. No PR extracts treatment-related abnormal clinical signs, body weight changes and mortalities were detected. Significant (p<0.01) increases of the numbers of polychromatic erythrocytes contain micronucleus in prepared bone marrow cells were detected in CPA and PR extracts 2000 mg/kg treated groups as compared with intact control, respectively. The results of intraperitoneal dose mouse bone marrow cell micronucleus test of PR extracts were positive in the present study. It is considered that there were no problems from cytotoxicity of PR extracts tested in this study because the polychromatic erythrocyte ratio was detected as > 0.42 in all tested groups.