Korean Journal of Oriental Medicine (한국한의학연구원논문집)

Korean Institute of Oriental Medicine (한국한의학연구원)
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Micronucleus Test of Pinella Rhizoma Aqueous Extracts in Bone Marrow Cells of Male ICR Mice - In Vivo Genotoxicity

반하 물 추출물의 ICR 마우스 골수세포를 이용한 소핵실험

  • Park, Ji-Ha (Department of Herbalogy, College of Oriental Medicine, Daegu Haany University)
  • 박지하 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2009.10.26
  • Accepted : 2009.12.22
  • Published : 2009.12.31
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Abstract

In this research, the genotoxic effects of Pinelliae Rhizoma (PR) extracts, one of famous herbal agents in Korean medicine were evaluated using the mouse micronucleus test. PR extracts was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000, and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. No PR extracts treatment-related abnormal clinical signs, body weight changes and mortalities were detected. Significant (p<0.01) increases of the numbers of polychromatic erythrocytes contain micronucleus in prepared bone marrow cells were detected in CPA and PR extracts 2000 mg/kg treated groups as compared with intact control, respectively. The results of intraperitoneal dose mouse bone marrow cell micronucleus test of PR extracts were positive in the present study. It is considered that there were no problems from cytotoxicity of PR extracts tested in this study because the polychromatic erythrocyte ratio was detected as > 0.42 in all tested groups.

Keywords

References

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