• 한국자원식물학회지
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• 제25권6호
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• pp.670-681
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• 2012

Herb extracts commercially used in Korea were screened for PPAR-${\gamma}$ agonist test and ${\alpha}$-glucosidase inhibition assay. Total 16 herb plants had a PPAR-${\gamma}$ agonist activity. Specially, Alisma orientale Juz (108.41%), Ephedra sinica (98.22%), Sasa japonica Makino var. purpurascens Nakai (140.68%), Astragalus membranaceus Bunge (106.79%) and Cnidium officinale Makino (113.00%) showed high PPAR-${\gamma}$ agonist activity rate compared with rosiglitazone's (167.46%). And Cornus officinalis S. et Z. (90.3%), Cinnamomum cassia Blume (89.2%), Psoralea corylifolia L. (89.8%), Paeonia japonica (Makino) Miyabe (92.4%) and Paeonia suffruticosa Andr (93.2%), showed high ${\alpha}$-glucosidase inhibition rates. These results support previous reports of the efficacy of Oriental medicinal plants used for diabetes mellitus.

• 한국자원식물학회지
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• 제26권2호
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• pp.214-226
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• 2013

본 연구는 희귀식물로 지정되어 있는 애기송이풀의 자생지 환경을 조사하여 보전 및 복원 시 기초자료를 제공하고자 한다. 조사결과 애기송이풀의 자생지는 해발고도 107~494m 범위와 경사 $5{\sim}20^{\circ}$의 계곡부에 주로 생육하는 것으로 조사되었다. 식생 및 관속식물 조사결과 6개 지역의 18개 방형구 내에서 조사된 관속식물은 총 243분류군이 출현하였으며, 각 조사구 내 초본층의 피도와 빈도를 기초로 한 애기송이풀의 중요치를 산출한 결과 평균 13%로 나타났다. NMS 분석결과, 애기송이풀 자생지에 결정적 영향을 미치는 인자는 목본층의 종구성, 초본층의 종구성보다는 환경적 영향이 더 지배적이라는 말할 수 있다. 이에 자생지의 안정적인 유지를 위해서는 서식지 보전이 중요하며 서식지 주변에 보호구역을 설정하는 것이 필요하다고 판단된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2005년도 춘계 학술발표대회
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• pp.5-16
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• 2005

The herb of Allium victorialis var. platyphyllum (Liliaceae) has been used as an edible wild herb and to treat heart failure and gastritis. We have already reported antihyperlipidemic anti-tumor effects of this plant. To enlarge the commercial availability of this food, it was investigated whether the extracts of A. victorialis var.platyphyllum reduce hyperlipidemia and obesity or not. The plants tested in this experiment were collected from two eco-types of IS. Ullung and Mt. Odae cultivated at Pyongchang. Extracts were prepared by extracting the fresh leaves and those dried at $36^{\circ}C$ and $90^{\circ}C$, respectively. Pretreatment with the ethanolic extracts for two weeks (p.o.) reduced serum triglyceride-, total cholesterol- and LDL-cholesterol contents in rats induced by Triton WR-1339, respectively. Furthermore, oral administration of the extracts also inhibited the hyperlipidemia induced with oral diet of 30% corn oil. In the other attempt to find to alleviate the obesity, the model rats with obesity were induced by the high fat-diet for six weeks. Post-treatment with the extracts for two weeks significantly reduced the hyperlipidemia. Retroperitoneal-, epidymal- and total abdominal fat pad weights were considerably reduced at 100 mg/kg oral administration of the extracts. Increased feces lipid contents were also found in the rat treated with the extracts. The extract of Mt. Odae eco-type showed more potent activity than that of Is. Ullung one. These results suggest that use of the fresh leaves may lead to the higher activity in treatment of hyperlipidemia and obesity than of the dried one.

• 한국약용작물학회지
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• 제22권6호
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• pp.489-496
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• 2014

Polygonatum is a genus placed in the family Liliaceae, distributed throughout the Northern Hemisphere and 16 of the species are grown naturally in Korea. In oriental medicine, the rhizomes of Polygonatum have been used as two different medicines, Okjuk (Polygonati odorati Rhizoma) and Hwangjeong (Polygonati Rhizoma). However, it is difficult to identify the morphological and chemical differences between the medicinal groups and thus easy to confuse the one with the other. Therefore, a clear classification standard needs to be established so as to be able to discriminate between them. In the study, the morphological characteristics of the plants, Polygonatum spp., were examined. Then, the differences in SNPs among the DNA sequences of 7 of the Polygonatum spp. and 1 of the Disporum spp. were analyzed by DNA barcoding with rpoC1, rpoB2, matK, and psbA-trnH of the cpDNA region. In the results, three regions, rpoC1, rpoB2, and matK were useful for discriminating the species, P. stenophyllum and P. sibiricum. Furthermore, it was possible to discriminate the individual germplasm within the species by using the combination of the results obtained from rpoB2, rpoC1, and matK.

• Journal of Animal Science and Technology
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• 제54권3호
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• pp.185-190
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• 2012

A total of 800 one-day-old chickens (Cobb 500) were obtained from a local hatchery and allocated randomly to five dietary treatments. This study conducted for 42 d. Treatment groups included 1) CON (control), 2) T0.3 (0.3% of thyme extract), 3) T0.6 (0.6% of thyme extract), 4) G0.3 (0.3% of garlic extract) and 5) G0.6 (0.6% of garlic extract). Thyme extract included $90{\mu}g/ml$ Carvacrol and 400 mg/ml thymol while garlic extract included 100 mg/ml elastin. There were no significant effects among the treatments on weight gain, feed intake and feed conversion ratio over the entire trial. Also, no significant differences were observed between CON group and other treatments in plasma parameters (cholesterol, triglyceride and HDL) and hematocrit. Sex of chickens had no effect on blood parameters. Supplementation of herb extracts did not affect carcass characteristics including carcass yield, breast, thigh and abdominal fat. There were no significant differences observed in carcass characteristics between males and females fed with different levels of herb extracts. In conclusion, supplementation of garlic and thyme extracts as feed additives at two levels of 3 and 6% in the present study did not improve performance and carcass traits of broiler chicks.

• 한국환경복원기술학회지
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• 제10권2호
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• pp.16-25
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• 2007

The purpose of the study is to analyse and classify the characteristics of Gongsanseong forest through phytosociological and quadrat method with 20 plot samples. In result, the forest was classified as Quercus acutissima community. The subcommunity was composed of Quercus aliena and Zelkova serrata. The average vegetation coverage of Quercus aliena subcommunity was upper tree layer (83%), lower tree layer (48%), shrub layer (39%), and herb layer (30%), while the average vegetation coverage of Zelkova serrata subcommunity was upper tree layer (76%), lower tree layer (52%), shrub layer (40%), and herb layer (45%). The order of importance value of the forest community with DBH 2cm above plants was Quercus acutissima (72.62), Quercus aliena (40.52), Prunus sargentii (19.81), Styrax japonica (19.39), Zelkova serrata (15.78), Robonia pseudoacacia (14.76), Quercus variabilis (13.83), Sorbus alnifolia (13.71), Platycarya strobilacea (10.74), Pinus densiflora (10.08), and Quercus serrata (9.31). According to breast diameter analysis results, it is expected that the importance value of Prunus sargentii and Zelkova serrata will be continuously increased while Quercus sp. is dominating the forest. With the result of ordination analysis, the relationship between the forest community and environmental factors could be affected by soil nutrition, moisture, and elevation etc.

• 한국축산식품학회지
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• 제31권2호
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• pp.304-310
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• 2011

본 연구에서는 한국전통 음식인 쇠고기와 돼지고기 떡갈비의 레시피에 묵은지 배추김치를 첨가하여 제품을 개발하였다. 묵은지 김치 첨가 떡갈비 제조방법을 확정하기 위한 연구로서, 특히 묵은지의 냄새를 완화하고, 기능성을 강화시키기 위하여 묵은지 요리에 허브를 첨가하였다. 대표적인 허브 종류별로 관능검사를 실시한 결과 허브의 선호도는 rosemary > lemon balm > thyme 순으로, rosemary가 가장 적합한 것으로 나타났다. 묵은지 배추김치를 이용한 요리의 대상으로 쇠고기와 돼지고기 떡갈비를 선정하였으며, 관능검사를 통하여 최적의 김치의 첨가 함량을 알아내었고 최적 배합비를 확립하였다. 묵은지 배추김치를 첨가한 쇠고기와 돼지고기 떡갈비를 제조하여 관능검사를 실시한 결과 종합적인 기호도 측면에서 쇠고기 떡갈비의 묵은지 김치 첨가 함량은 10% 첨가군이 가장 우수하였으며. 돼지고기 떡갈비의 묵은지 김치 첨가 함량은 20% 첨가군이 가장 우수하였다.

• 한국자원식물학회지
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• 제28권5호
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• pp.582-590
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• 2015

본 연구는 HRL의 3T3-L1 지방전구세포의 분화과정 중에 HRL이 지방의 축적에 미치는 영향을 확인하였다. MTT assay를 이용하여 세포 독성을 측정한 결과 100 ㎍/㎖의 농도에서도 세포증식에 영향을 미치지 않는 것을 확인하였고, 이와 같은 결과를 토대로 Oil Red O 염색법을 이용하여 지방세포 분화 억제능을 측정하였다. 그 결과, HRL의 경우 100 ㎍/㎖의 농도에서 82.25% 지방 축적 억제능을 나타내었다. 지방생성에 영향을 미치는 유전자 발현량을 측정하기 위해 RT-PCR법과 western blot법을 시행하였다. HRL은 SREBP-1c, PPARγ와 C/EBPα의 mRNA 발현을 억제시켰고, 지방생성에 영향을 미치는 효소인 FAS의 생성을 조절하는 것으로 나타났다. 또한, HRL 처리로 AMPKα의 단백질 발현이 증가하였으며, PPARγ의 발현량이 감소하는 것을 확인하였다. 이상의 결과들로부터 HRL은 AMPKα의 활성화를 통한 지방 합성을 억제를 보유하고 있는 바, 향후 항비만 기능성 소재로 활용될 수 있을 것으로 생각한다.

• 한국자원식물학회지
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• 제30권3호
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• pp.323-330
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• 2017

Eleutherococcus senticosus (Siberian ginseng) is an important medicinal tree found in northeast Asia. In this study, we analyzed the genome-wide distribution of microsatellites in E. senticosus. By sequencing 711 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in E. senticosus accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 131 E. senticosus accessions in Korea and China. The number of alleles ranged from 2 to 11, with an average of 7.4 alleles. The mean values of observed heterozygosity ($H_O$) and expected heterozygosity ($H_E$) were 0.59 and 0.56, respectively. The average polymorphism information content (PIC) was 0.51 in all 131 E. senticosus accessions. E. senticosus accessions in Korea and China showed a close genetic similarity. Significantly low pairwise genetic divergence was observed between the two regions, suggesting a relatively narrow level of genetic basis among E. senticosus accessions. Our results not only provide molecular tools for genetic studies in E. senticosus but are also helpful for conservation and E. senticosus breeding programs.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.90-90
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• 2019

This study was carried out to investigate the vegetation and vegetation structure of Mt. Man in Hwacheon Gun from April to October 2018. The survey site is located in the northern part of South Korea where the climate is similar to that of North Korea. The purpose of this study is to provide basic information for efficient ecological forest management after unification. A total of 341 vascular plant taxa are recorded, representing 221 genera, 4 subspecies, 42 varieties and 6 forms in 84 families. Rare species, including Crataegus komarovii and Amitostigma gracile are found across 10 taxa; 8 families, 10 genera, 9 species, 1 variety. Naturalized Plants, including Ambrosia artemisiifolia and Erigeron annuus found across 11 taxa; 6 families, 10 genera, 11 species. Dominant tree species of the tree layer based on the highest importance values were: Quercus mongolica (23.47%), Larix kaempferi (11.43%), Fraxinus rhynchophylla (8.30%), in the subtree layer were: Lindera obtusiloba (11.38%), Acer pictum subsp. mono (11.33%), Fraxinus rhynchophylla (9.80%) and in the shrub layer were: Lindera obtusiloba (10.22%), Fraxinus rhynchophylla (8.43%), Rubus crataegifolius (8.00%), in the herb layer Pteridium aquilinum var. latiusculum (5.67%), Oplismenus undulatifolius (5.00%), Lysimachia clethroides (3.49%) respectively. The species diversity of Mt. Man was 1.1764 in the tree layer, 1.2403 in the subtree layer, 1.4135 in the shrub layer, and 1.7416 in the herb layer. In conclusion, if we utilize the flora and vegetation structure surveyed in Mt. Man, we can effectively manage the degraded forests with similar altitudes and climate zones in North Korea.