Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Development of Microsatellite Markers and their Use in Genetic Diversity and Population Analysis in Eleutherococcus senticosus

  • Lee, Kyung Jun (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • An, Yong-Jin (Ginseng & Medicinal Herb Experiment Station) ;
  • Ham, Jin-Kwan (Ginseng & Medicinal Herb Experiment Station) ;
  • Ma, Kyung-Ho (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Jung-Ro (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Cho, Yang-Hee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Gi-An (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
  • Received : 2017.03.27
  • Accepted : 2017.05.04
  • Published : 2017.06.30
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Abstract

Eleutherococcus senticosus (Siberian ginseng) is an important medicinal tree found in northeast Asia. In this study, we analyzed the genome-wide distribution of microsatellites in E. senticosus. By sequencing 711 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in E. senticosus accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 131 E. senticosus accessions in Korea and China. The number of alleles ranged from 2 to 11, with an average of 7.4 alleles. The mean values of observed heterozygosity ($H_O$) and expected heterozygosity ($H_E$) were 0.59 and 0.56, respectively. The average polymorphism information content (PIC) was 0.51 in all 131 E. senticosus accessions. E. senticosus accessions in Korea and China showed a close genetic similarity. Significantly low pairwise genetic divergence was observed between the two regions, suggesting a relatively narrow level of genetic basis among E. senticosus accessions. Our results not only provide molecular tools for genetic studies in E. senticosus but are also helpful for conservation and E. senticosus breeding programs.

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