Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Morphological Characteristics and Phylogenetic Analysis of Polygonatum Species Based on Chloroplast DNA Sequences

한국산 둥굴레속 식물의 형태적 특성 및 엽록체 DNA 염기서열을 이용한 유연관계 분석

  • Kim, Jeong Hun (Department of Biological & Environmental Science, Dongguk University) ;
  • Seo, Jae Wan (Department of Biological & Environmental Science, Dongguk University) ;
  • Byeon, Ji Hui (Department of Biological & Environmental Science, Dongguk University) ;
  • Ahn, Young Sup (Department of Herb Crop Resources, NIHHS, RDA) ;
  • Cha, Seon Woo (Department of Herb Crop Resources, NIHHS, RDA) ;
  • Cho, Joon Hyeong (Department of Biological & Environmental Science, Dongguk University)
  • 김정훈 (동국대학교 바이오환경과학과) ;
  • 서재완 (동국대학교 바이오환경과학과) ;
  • 변지희 (동국대학교 바이오환경과학과) ;
  • 안영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조준형 (동국대학교 바이오환경과학과)
  • Received : 2014.10.24
  • Accepted : 2014.11.12
  • Published : 2014.12.30
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Abstract

Polygonatum is a genus placed in the family Liliaceae, distributed throughout the Northern Hemisphere and 16 of the species are grown naturally in Korea. In oriental medicine, the rhizomes of Polygonatum have been used as two different medicines, Okjuk (Polygonati odorati Rhizoma) and Hwangjeong (Polygonati Rhizoma). However, it is difficult to identify the morphological and chemical differences between the medicinal groups and thus easy to confuse the one with the other. Therefore, a clear classification standard needs to be established so as to be able to discriminate between them. In the study, the morphological characteristics of the plants, Polygonatum spp., were examined. Then, the differences in SNPs among the DNA sequences of 7 of the Polygonatum spp. and 1 of the Disporum spp. were analyzed by DNA barcoding with rpoC1, rpoB2, matK, and psbA-trnH of the cpDNA region. In the results, three regions, rpoC1, rpoB2, and matK were useful for discriminating the species, P. stenophyllum and P. sibiricum. Furthermore, it was possible to discriminate the individual germplasm within the species by using the combination of the results obtained from rpoB2, rpoC1, and matK.

Keywords

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