• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.668-673
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• 2013

A recombinant E. coli co-expressing ALA synthase (hemA), NADP-dependent malic enzyme (maeB), and dicarboxylic acid transporter (dctA) was reported to synthesize porphyrin derivatives including iron-containing heme. To enhance the synthesis of bacterial heme, five genes of the porphyrin biosynthetic pathway [pantothenate kinase (coaA), ALA dehydratase (hemB), 1-hydroxymethylbilane synthase (hemC), uroporphyrinogen III synthase (hemD), and uroporphyrinogen III decarboxylase (hemE)] were amplified in the recombinant E. coli co-expressing hemA-maeB-dctA. Pantothenate kinase expression enabled the recombinant E. coli to accumulate intracellular CoA. Intracellular ALA was the most enhanced by uroporphyrinogen III synthase expression, porphobilinogen was the most enhanced by ALA dehydratase expression, uroporphyrin and coproporphyrin were the most enhanced by 1-hydroxymethylbilane synthase expression. The strain co-expressing coaA, hemA, maeB, and dctA produced heme of $0.49{\mu}mol/g$-DCW, which was twice as much from the strain without coaA expression. Further pathway gene amplifications for the porphyrin derivatives are discussed based on the results.

• Journal of Microbiology and Biotechnology
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• v.17 no.9
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• pp.1579-1584
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• 2007

5-Aminolevulinate (ALA) synthase (E.C. 2.3.1.37), which mediates the pyridoxal phosphate-dependent condensation of glycine and succinyl-CoA, encoded by the Rhodobacter sphaeroides hemA gene, enables Escherichia coli strains to produce ALA at a low level. To study the effect of the enhanced C4 metabolism of E. coli on ALA biosynthesis, NADP-dependent malic enzyme (maeB, E.C. 1.1.1.40) was coexpressed with ALA synthase in E. coli. The concentration of ALA was two times greater in cells coexpressing maeB and hemA than in cells expressing hemA alone under anaerobic conditions with medium containing glucose and glycine. Enhanced ALA synthase activity via coupled expression of hemA and maeB may lead to metabolic engineering of E. coli capable of large-scale ALA production.

• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.976-983
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• 2003

Seven Escherichia coli cells defective with aerobic growth were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created a transcriptional fusion to lacZY. These insertion mutant cells were tested on an XG ($5-bromo-4-chloro-3-indolyl-{\beta}-D-galactopyranoside$) medium for anaerobic expression of lacZ by fusion to a promoter. The chromosomal DNA from these strains were digested by EcoRI, and the EcoRI fragments that contained the fused gene and lacZ sequence were identified by Southern hybridization, using lacZ containing plasmid as a probe. The EcoRI fragment from each strain was cloned and sequenced. The sequence data were compared with the GenBank database. The mutated gene of three strains, CYT4, CYT5, and OS11, was found to be identical, and it was nrdAB that encoded ribonucleoside diphosphate reductase. The gene nrdAB was at min 50.5 on the Escherichia coli linkage map and 2,348,084 on the physical map, and is involved in hemAe-related reduction-oxidation reaction. OS6 and OS14 mutant strains had insertion at min 8.3 and the mutated gene was hemB. The hemB encodes 5-aminolevulinate dehydratase or porphobilinogen synthase. The OS3 mutant had insertion in cydB at min 16.6. The cydAB encodes cytochrome d oxidase. In the case of OS1, the fusion was made with sucA, the E1 component of ${\alpha}-ketoglutarate$ dehydrogenase.

• Journal of the Korea Fashion and Costume Design Association
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• v.17 no.2
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• pp.223-237
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• 2015

This study is to compare the pattern making methods of men's jeans and to analyze sizes of parts while also evaluating the appearance and fit according to a 3D virtual garment simulation. Five types of patterns are selected for the analysis. For the appearance evaluation, the study conducted a virtual clothing simulation, created a perspective drawing, and applied Clothing pressure to each test outfit. First, according to the pattern comparisons, A and D had the shortest crotch length, while B had the longest; B had the narrowest crotch width, while C had the widest; C had the greatest waist circumference, and B had the smallest; C had the greatest knee and hem circumference, while E had the smallest. Second, according to the appearance evaluation, E pants had the highest rating overall for the waist, crotch, knee, and hem comfort; A pants had the highest rating for the yoke line end and placement; and C pants had the highest rating for the side seam line. According to the correlation analysis, ease of the waist, hips, hem and thighs were important factors (in that order) in determining the overall appearance of the jean pants.

• The Research Journal of the Costume Culture
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• v.23 no.2
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• pp.284-293
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• 2015

This study intends to analyze the fit of directly-imported American casual pants for Korean female college students in terms of appearance and comfortableness. The authors carried out a wearing test with sample pants from 3 brands (DKNY: A, Gap: B, Polo: C) that had the most market share and that were mostly preferred by Korean female college students. Results showed that pants A had insufficient ease in the crotch, as the depth of the crotch was short. The hip girth received a low assessment in terms of appearance. Moreover, the hem widths were wide while the pants length was long. Pants B were expected to be most appropriate for Korean women's bodies, as they had the longest crotch depth among the three brands. However, the pants appeared to pull downward due to the narrow thigh and the knee girth and hem width. Moreover, the crotch girth and hip girth received a poor appearance assessment. Pants C were rated as satisfactory for their thigh girth, knee girth, and hem width. However, the crotch was uncomfortable, as it had a short crotch depth. The hip girth received a low appearance assessment. This study suggests that a pattern adjustment is required for directly-imported American pants to provide a better fit for the bodies of Korean women. The crotch depths should be approximately 17.5 cm. The hem widths should be approximately 20 cm. We also suggest a leg length of 75 cm, a waist girth of 86 cm, and a hip girth of 97 cm.

• Microbiology and Biotechnology Letters
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• v.49 no.2
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• pp.157-166
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• 2021

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

• Journal of Navigation and Port Research
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• v.33 no.3
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• pp.181-191
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• 2009

In general, seafarer's human error is considered to be the preponderant muse for the majority of maritime transportation accidents in a merchant ship. The implementation techniques for Human Error Model (HEM) to assess possible accident risk by deck officers including captain, chief officer, second mate and third mate are described in this study. The scope of this work is focused to 642 deck officers in the ship management company with 130 vessels. At first, HEM can be constructed through the statistical analysis and expert's brainstorming process with human data to 642 deck officers. Then the variables $\upsilon$ for the human factors, the evaluation level EP($\upsilon$) for $\upsilon$, the weight $\alpha$ of $\upsilon$, and the title weight $\beta$ of each deck officers can be decided. In addition, through the analysis of ship's accident history, the accident causation ratios by human error ${\gamma}_H$ and by external error ${\gamma}_B$ can be found as 0.517(51.7%) and 0.483(48.3%), respectively. The correlation coefficients to $\upsilon$ are also shown significant for a 95% confidence interval (p < 0.05) for each coefficient. And the validity of HEM is also surveyed by the analysis of normal probability distribution of risk level RL to each deck officer.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2007.06a
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• pp.569-571
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• 2007

In this paper, hem antenna composed of rectangular hern, circular polarizer and straight-type mode convertor is designed and fabricated. The circular polarizer is designed as a dielectric type having broadband characteristic, and it is inserted into the rectangular hem with $45^{\circ}$ inclination. The straight-type mode convertor has a terrace-type structure in order to convert the TEM mode signal of coaxial cable to the TE mode of rectangular waveguide, and this structure minimizes conversion loss. Fabricated hem antenna operates at $30.085\sim30.885GHz$ and has VSWR < 1.5, Axial ratio < 1.0dB and antenna gain of $6.7\sim7.0dBi$ in the band.

• Proceedings of the KIEE Conference
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• 1999.07e
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• pp.2086-2088
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• 1999

765kv Transmission lines of the contaminated areas need many insulators due to the contamination. So the length of the arcing hem is very long. Because it is very difficult to install the arcing hem on insulator string sets due to its length, we must install the arcing horn on the jumping devices, and studied about the jumper horn. This paper described the investigation and analysis of the installation status of the arcing horn, and the design of the jumper horn for 765 kV transmission lines.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09b
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• pp.1119-1120
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• 2006

High Energy Milling (HEM) is applied for the grinding of cement and this can lead to substantial refinement $(<2{\mu}m)$ and mechanically activation of the powder particles. The present paper reviews the preliminary studies, explains the novel technique and suggests the route into commercial application. Particular attention is paid to wear results with an applied $Si_3N_4-grinding$ unit where no substantial wear was found after 4000 h of operation.