• The Journal of Engineering Geology
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• v.5 no.3
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• pp.277-288
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• 1995

Fracture detection has always been very attractive to the log, because it is important in many of our prospecting activities, e.g. in understanding the underground rock formation and also the fluid flow as a high permeability path. This paper demonstrates the use of high resolution borehole acoustic scanner for the detection of fractures. The tool, known as Televiewer, is the first acoustic borehole imaging system to use a focussed beam. The acoustic beams generated by a single transducer are sent toward the borehole wall, scanning the wall in a tight helix as the tool moves along the borehole. The amplitudes and travel times of the reflected signals are then measured, which produces the corresponding images. The highly resolved amplitude image allows to recognize various size of fractures and in addition to derive the rock strength from the image. Meanwhile, the travel time image itself can be directly converted to a precise caliper image, providing detailed information of deviations of the borehole shape. It also allows correction of and explanations for amplitude variations. Field measurements were carried Out at the Cheongyang study sites in Korea to illustrate the efficiency of the televiewer log.

• Applied Biological Chemistry
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• v.33 no.4
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• pp.343-348
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• 1990

E. coli LE392, transformed with CDDP-treated pBR322 DNA, was plated on ampicillin containing media. The number of colonies formed on ampicillin containing agar plate was reduced to undetectable level after treat the DNA with 13.3 ${\mu}M$ CDDP. The CDDP-treated pBR322 DNA was susceptible to sing1e strand DNA specific S1 nuclease and it's migration Pattern in agarose gel electrophoresis was changed. These results suggest that CDDP adduction to pBR322 DNA resulted in denaturation of the double helix and changes in it's conformation which ultimately leads In the inactivation of the ampicillin resistant sere.

• Journal of the Economic Geographical Society of Korea
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• v.19 no.1
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• pp.18-32
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• 2016

In the Hyper-connected society, each country set up its own policy and central government as well as provincial government makes a basic plan of developing IoT. Gyeonggi provincial government needs to cope actively with the changing international and national circumstances. The purpose of this paper is to frame policy as a provincial government with analysis IoT industry-academia-institute-governments ecosystem and in-depth interview. There are IoT related SMEs in Gyeonggi, especially manufacturing business and device fields. Universities are doing IoT researches by R&D funds from central as well as provincial governments. Central government-affiliated Institutions are researching. It is necessary for Gyeonggi provincial government to establish policy in order to actively operate IoT ecosystem while each innovation actors are cooperated in doing IoT; system/governce maintenance, environments and test-bed for the application.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.4
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• pp.289-295
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• 2005

In this study, the cultural medium used for the efficient production of $\gamma$-PGA with a newly isolated Bacillus sp. RKY3 was optimized. It was necessary to supplement the culture medium with L-glutamic acid and an additional carbon source in order to induce the effective production of $\gamma$-PGA. The amount of $\gamma$-PGA increased with the addition of L-glutamic acid to the medium. The addition of 90 g/L L-glutamic acid to the medium resulted in the maximal yield of $\gamma$-PGA (83.2 g/L). The optimum nitrogen source was determined to be peptone, but corn steep liquor, a cheap nutrient, was also found to be effective for $\gamma$-PGA production. Both the $\gamma$-PGA production and cell growth increased rapidly with the addition of small amounts of $K_2HPO_4$ and $MgSO_4\cdot7H_{2}O$. Bacillus sp. RKY3 appears to require $Mg^{2+}$, rather than $Mn^{2+}$, for $\gamma$-PGA production, which is distinct from the production protocols associated with other, previously reported bacteria. Bacillus sp. RKY3 may also have contributed some minor $\gamma$-PGA depolymerase activity, resulting in the reduction of the molecular weight of the produced $\gamma$-PGA at the end of fermentation.

• BMB Reports
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• v.43 no.8
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• pp.547-553
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• 2010

Biochemical tests such as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are useful for diagnosing patients with liver disease. In this study, we tested the association between copy number variation and the hepatic biomarkers AST and ALT based on 8,842 samples from population-based cohorts in Korea. We used Affymetrix Genome-Wide Human 5.0 arrays and identified 10,534 CNVs using HelixTree software. Of the CNVs tested using univariate linear regression, 100 CNVs were significant for AST and 16 were significant for ALT (P < 0.05). We identified 39 genes located within the CNV regions. DKK1 and HS3ST3B1 were shown to play roles in heparan sulfate biosynthesis and the Wnt signaling pathway, respectively. NAF1 and NPY1R were associated with glycoprotein processes and neuropeptide Y receptor activity based on GO categories. PTER, SOX14 and TM7SF4 were expressed in liver. DPYS and CTSC were found to be associated with dihydropyrimidinuria and Papillon-Lefevre syndrome phenotypes using OMIM. NPY5R was found to be associated with dyslipidemia using the Genetic Association Database.

• Molecules and Cells
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• v.27 no.6
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• pp.673-680
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• 2009

Conversion of the normal soluble form of prion protein, PrP ($PrP^C$), to proteinase K-resistant form ($PrP^{Sc}$) is a common molecular etiology of prion diseases. Proteinase K-resistance is attributed to a drastic conformational change from ${\alpha}$-helix to ${\beta}$-sheet and subsequent fibril formation. Compelling evidence suggests that membranes play a role in the conformational conversion of PrP. However, biophysical mechanisms underlying the conformational changes of PrP and membrane binding are still elusive. Recently, we demonstrated that the putative transmembrane domain (TMD; residues 111-135) of Syrian hamster PrP penetrates into the membrane upon the reduction of the conserved disulfide bond of PrP. To understand the mechanism underlying the membrane insertion of the TMD, here we explored changes in conformation and membrane binding abilities of PrP using wild type and cysteine-free mutant. We show that the reduction of the disulfide bond of PrP removes motional restriction of the TMD, which might, in turn, expose the TMD into solvent. The released TMD then penetrates into the membrane. We suggest that the disulfide bond regulates the membrane binding mode of PrP by controlling the motional freedom of the TMD.

• Molecules and Cells
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• v.27 no.1
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• pp.39-45
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• 2009

Ligand-dependent or independent oligomerization of receptor protein tyrosine kinase (RPTK) is often an essential step for receptor activation and intracellular signaling. The novel oncogene with kinase-domain (NOK) is a unique RPTK that almost completely lacks an ectodomain, expresses intracellularly and activates constitutively. However, it is unknown whether NOK can form oligomer or what function oligomerization would have. In this study, two NOK deletion mutants were generated by either removing the ectodomain ($NOK{\Delta}ECD$) or including the endodomain (NOK-ICD). Co-immunoprecipitation demonstrated that the transmembrane (TM) domain of NOK was essential for its intermolecular interaction. The results further showed that NOK aggregated more closely as lower order oligomers (the dimer- and trimer-sized) than either deletion mutant did since NOK could be crosslinked by both Sulfo-EGS and formaldehyde, whereas either deletion mutant was only sensitive to Sulfo-EGS. Removing the NOK TM domain (NOK-ICD) not only markedly promoted higher order oligomerization, but also altered the subcellular localization of NOK and dramatically elevated the NOK-mediated constitutive activation of extracellular signal-regulated kinase (ERK). Moreover, NOK-ICD but not NOK or $NOK{\Delta}ECD$ was co-localized with the upstream signaling molecule RAS on cell membrane. Thus, TM-mediated intermolecular contacting may be mainly responsible for the constitutive activation of NOK and contribute to the autoinhibitory effect on RAS/MAPK signaling.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.159-161
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• 2005

Hypovirus infection of the chestnut blight fungus Cryphonectria parasitica is a useful model system to study the hypoviral regulation of fungal gene expression. The hypovirus is known to downregulate the fungal laccase1 (lac 1), the modulation of which is tightly governed by the inositol triphosphate ($IP_3$) and calcium second messenger system in a virus-free strain. We cloned the gene cplc1 encoding a phosphatidyl inositol-specific phospholipase C (PLC), in order to better characterize the fungal gene regulation by hypovirus. Sequence analysis of the cplc1 gene indicated that the protein product contained both the X and Y domains, which are the two conserved regions found in all known PLCs, with a 133 amino acid extension between the 2nd ${\beta}$-strand and the ${\alpha}$-helix in the X domain. In addition, the gene organization appeared to be highly similar to that of a ${\delta}$ type PLC. Disruption of the cplc1 gene resulted in slow growth and produced colonies characterized by little aerial mycelia and deep orange in color. In addition, down regulation of lac1 expression was observed. However, temperature sensitivity, osmosensitivity, virulence, and other hypovirulence-associated characteristics did not differ from the wild-type strain. Functional complementation of the cplc1-null mutant with the PLC1 gene from Saccharomyces cerevisiae restored lac1 expression, which suggests that the cloned gene encodes PLC activity. The present study indicates that the cplc1 gene is required for appropriate mycelial growth, and that it regulates the lac1 expression, which is also modulated by the hypovirus. Although several PLC genes have been identified in various simple eukaryotic organisms, the deletion analysis of the cplc1 gene in this study appears to be the first report on the functional analysis of PLC in filamentous fungi.

• Journal of Plant Biology
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• v.30 no.1
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• pp.43-57
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• 1987

The epidermal structure and stomatal types in vegetative and reproductive organs of three species of Bryophyllum(B. crenatum, B. diagremontion, B. tubiflorum) were described. The epidermal cells were polygonal, isodiametric, and rectangular in the leaves and stems, and elongated cells in the stamens, styles, and ovaries. These cells were commonly thick, and arched or sinuous in the leaves, epiphylous bunds, petals and ovaries. They were straight in the stems, petioles, pedicels, and peduncles. In both vegetative and reproductive organs, the subsidiary cell walls were commonly thin and mostly arched in all the organs. The great majority of the mature stomata in all the organs were helicocytic type with a helix of four to six subsidiary cells. The mature stomata varied from organ to organ with regard to the number and arangement of subsidiary cells. The ontogenetic type of stomata in all the organs was mostly helico-eumesogenous type. This type was subdivided into three subtypes such as parahelico-eumesogenous, anomohelico-eumesogenous, and diahelico-eumesogenous stomata on the basis of the division angle fo the guard mother cell. Sometimes, the anisoeumesogenous type was found in various organs. This type was subdivided into three subtypes such as paraniso-eumesogenous, anomoaniso-eumesogenous, and dianisoeummesogenous stomata. The tetra-eumesogenous and duplotetra-eumesogenous types were rarely found; the former in the leaf of B. crenatum and the latter in the leaf of B. diagremontiana. Anomometric patterns in the mesogenous categorry of stomatal types was observed in a few organs of all the materials. A new stomatal type with tetra-eumesogenous stoma within a girdle of three subsidiary cells fo aniso-eumesogenous in the leaf of B. diagremontiana was firstly observed in the vascular plants. This stoma was termed the cotetra-aniso-eumesogenous type. Anormal stomata such as aborted stomata, single guard cells, stoma with a constricted part in the middle of large guard cells, and arrested stomata were found in the various organs of all the materials.

• BMB Reports
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• v.33 no.2
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• pp.120-125
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• 2000

M2PI is an active single chain mini-proinsulin with a 9-residue linker containing the turn-forming sequence 'YPGDV' between the B- and A-chains, but which retains about 50% of native insulin receptor binding activity. The refolding efficiency of M2PI is higher than proinsulin by 20-40% at alkaline pH, and native insulin is generated by the enzymatic conversion of M2PI. The solution structure of M2PI was determined by NMR spectroscopy. The global structure of M2PI is similar to that of native insulin, but the flexible linker between the B- and A-chains perturbed the N-terminal A-chain and C-terminal B-chain. The helix in the N-terminal A-chain is partly perturbed and the ${\beta}$-turn in the B-chain is disrupted in M2PI. However, the linker between the two chains was completely disordered indicating that the designed turn was not formed under the experimental conditions (20% acetic acid). Considering the fact that an insulin analogue, directly cross-linked between the C-terminus of the B-chain and the N-terminus of the A-chain, has negligible binding activity, a flexible linker between the two chains is sufficient to keep binding activity of M2PI, but the perturbed secondary structures are detrimental to receptor binding.