Bradyrhizobium japonicum is a soil bacterium with a unique ability to infect the roots of leguminous plants and establish a nitrogen-fixing symbiosis, which has been used as a microbial manure. In this study, we examined the stress response after pretreatment of cells with cold temperature. When pre-treated with cold temperature ($4^{\circ}C$) for 16 hr, B. japonicum increased the viability in subsequent stress-conditions such as alcohol, $H_2O_2$, heat, and dehydration. For cold adpatation, cultured B. japonicum was exposed to $4^{\circ}C$. Upon subsequent exposure to various conditions, the number of adapted cells pretreated by cold adaptation was 10-1000 fold higher than that of non-adaptated ones. It appeared de novo protein synthesis occurred during adaptation, because a protein synthesis inhibitor, chloramphenicol abolished the increased stress tolerance. By using a degenerate PCR primer set, a csp homolog was amplified from B. japonicum genome and sequenced. The deduced partial amino acid sequence of the putative Csp (Cold shock protein) shares a significant similarity with known Csp proteins of other bacteria.
Korean Journal of Agricultural and Forest Meteorology
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v.3
no.4
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pp.220-237
/
2001
Over the 20th century global temperature increase has been 0.6$^{\circ}C$. The globally averaged surface temperature is projected to increase by 1.4 to 5.8$^{\circ}C$ over the period 1990 to 2100. Nearly all land areas will have higher maximum temperature and minimum temperature, and fewer cold days and frost days. More intense precipitation events will take plate over many areas. Over most mid-latitude continental interiors will have increased summer continental drying and associated risk of drought. By 2100, if the annual surface temperature increase is 3.5$^{\circ}C$, we will have 15.9$^{\circ}C$ from 12.4$^{\circ}C$ at present. Also the annual precipitation will range 1,118-2,447 mm from 972-1,841 mm at present in Korea. Consequently the average crop periods for summer crops will be 250 days that prolonged 32 days than at present. In the case of gradual increase of global warming, an annual crop can be adapted to the changing climate through the selection of filial generations in breeding process. The perennial crops such as an apple should be shifted the chief producing place to northern or high latitude areas where below 13.5$^{\circ}C$ of the annual surface temperature. If global warming happens suddenly over the threshold atmospheric greenhouse gases, then all ecosystems will have tremendous disturbance. Agricultural land-use plan, which state that farmers decide what to plant, based on their climate-based advantages. Therefore, farmers will mitigate possible negative imparts associated with the climate change. The farmers will have application to use agricultural meteorological information system, and agricultural long-range weather forecast system for their agroecosystems management. The ideal types of crops under $CO_2$ increase and climate change conditions are considered that ecological characteristics need indispensable to accomplish the sustainable agriculture as the diversification of genetic resources from yield-oriented to biomass-oriented characteristics with higher potential of $CO_2$ absorption and primary production. In addition, a heat-and-cold tolerance, a pest resistance, an environmental adaptability, and production stability should be also incorporated collectively into integrated agroecosystem.
Selection of oxygen-tolerant strains and elucidation of their oxygen tolerance mechanism were crucial for effective use of bifidobacteria. Oxygen-tolerant bifidobacteria were able to significantly remove environmental oxygen (oxygen removal activity) as compared to oxygen-sensitive strains. Most oxygen removal activity was inhibited by heat treatment and exposure to extreme pH (2.0) of bifidobacterial cell. NADH oxidase was major enzyme related to oxygen removal activity. Oxygen-tolerant bifidobacteria possessed high NADH peroxidase activity level to detoxify $H_2O_2$ formed from reaction of NADH oxidase. Addition of oxygen to anaerobic culture broth significantly increased activities of HADH oxidase and NADH peroxidase within 1hr and rapid increment of oxygen concentration was prevented. Results showed NADH oxidase and NADH peroxidase of oxygen-tolerant bifidobacteria played important roles in elimination of oxygen and oxygen metabolite $(H_2O_2)$.
Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and $TNF-{\alpha}$) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.
Transactions of the Korean Society of Mechanical Engineers B
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v.22
no.9
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pp.1317-1324
/
1998
In general, DI gasoline engine has the advantages of higher power output, higher thermal efficiency, higher EGR tolerance and lower emissions due to the operation characteristics of increased volumetric efficiency, compression ratio and ultra-lean combustion scheme. In order to apply the concept of stratified charge into direct injection gasoline engine, some kinds of methodologies have been adapted in various papers. In this study, a reflector was adapted around the injector nozzle to apply the concept of stratified charge combustion which leads the air-fuel mixture to be rich near spark plug. Therefore, the mixture near the spark plug is locally rich to ignite while the lean mixture is wholly introduced into the combustion chamber. The characteristics of combustion is analyzed with the variations of fuel injection pressure and load in a stratified -charge direct injection single cylinder gasoline engine. The obtained results are summarized as follows ; 1. The MBT spark timing approached to TDC with the increase of load on account of the increase of evaporation energy, but has little relation with fuel injection pressure. 2. The stratification effects are apparent with the increase of injection pressure. It is considered by the development of secondary diffusive combustion and the increase of heat release of same region, but proceed rapidly than diesel engine. Especially, in the case of high pressure injection (l70bar) and high load (3.0kgf m), the diffusive combustion parts are developed excessively and results in the decrease of peak pressure than in the case of middle load. 3. The index of engine stability, COVimep value, is drastically decreased with the increase of load. 4. To get better performance of DI gasoline engine development, staged optimizaion must be needed such as injection pressure, reflector, intake swirl, injection timing, chamber shape, ignition system and so on. In this study, the I50bar injection pressure is appeared as the optimum.
Proceedings of the Korean Society of Crop Science Conference
/
1998.10a
/
pp.313-335
/
1998
If the atmospheric concentrations of greenhouse gases double, the annual temperature increase in mean surface temperature relative to 1990 will be about 2.0 to $2.5^{\circ}C$ and the annual precipitation increase about $15{\%}$ by 2100 in Korea. When the temperature rises $2^{\circ}C$, the annual temperature will be $13^{\circ}C,\;15^{\circ}C,\;and\;16^{\circ}C$ in Western Central, Yeongnam Basin, and Southern Coastal respectively. Consequently the crop period could be prolonged $10{\~}29$ days. In the case of gradual global warming, annual crops could be adapted to the changed environment by breeding, and the perennial crops should be shifted to ether area. If global warming happens suddenly over the threshold of atmospheric greenhouse gases concentration, then we shall have disturbance of ecosystem. When $2^{\circ}C$ higher than present, the optimum flowering date of rice plant delayed about 10 days, so it may not possible to adopt the fate with present japonica rices, therefore, the recommended characteristics of rice varieties are longer basic vegetative period, more late maturing and higher ripening temperature. Barley and wheat crops could be shifted to northern coastal areas and apple production areas should be shifted to those areas under $13.5^{\circ}C$ in annual mean temperature at global warming. Ideotypes of crops under climate changes should have such ecological characteristics that are indispensable to accomplish the sustainable agriculture under increased $CO_{2}$ and temperature condition as the diversification of genetic resources from yield-oriented to biomass-oriented characteristics with the higher potentials of $CO_{2}$ absorption and Primary production. In addition, a heat-tolerance, a pest resistance, an environmental adaptability and a production stability should be also incorporated collectively into our integrated agroecosystem.
Kim, Hyun-Ju;Kang, In-Soon;Lee, Chin-Bum;Lee, Choon-Hwan;Cho, Sung-Ho;Moon, Byoung-Yong
Journal of Photoscience
/
v.12
no.1
/
pp.33-39
/
2005
Plants possess the ability to dissipate the excitation energy for the protection of photosynthetic apparatus from absorbed excess light. Heat dissipation is regulated by xanthophyll cycle in thylakoid membranes of chloroplasts. We investigated the mechanistic aspects of xanthophyll cycle-dependent photoprotection against low-temperature photoinhibition in plants. Using barley and rice as chilling-resistant species and sensitive ones, respectively, chilling-induced chlorophyll fluorescence quenching, composition of xanthophyll cycle pigments and mRNA expression of the zeaxanthin epoxidase were examined. Chilled barley plants exhibited little changes in chlorophyll fluorescence quenching either of photochemical or non-photochemical nature and in the photosynthetic electron transport, indicating low reduction state of PS II primary electron acceptor. In contrast to the barley, chilled rice showed a marked decline in those parameters mentioned above, indicating the increased reduction state of PS II primary electron acceptor. In addition, barley plants were shown to have a higher capacity to elevate the pool size of xanthophyll cycle pigments in response to cold stress compared to rice plants. Such species-dependent regulation of xanthophyll cycle activity was correlated with the gene expression level of cold-induced zeaxanthin epoxidase. Chilled rice plants depressed the gene expression of zeaxanthin epoxidase, whereas barley increased its expression in response to cold stress. We suggest that chilling-induced alterations in the pool size of xanthophyll cycle pigments related to its capacity would play an important role in regulating plant's sensitivity to chilling stress.
We have explored the importance of two ATP binding domains of Hsp104 protein in protection of yeast cells from cadmium exposure. In the previous study we have discovered that the presence of two ATP binding sites was essential in providing heat sh ock protection as well as rescuing cells from oxidative stress. In this paper we first report wild type cell with functional hsp104 gene is more resistant to cadmium stress than hsp104-deleted mutant cell, judging from decrease in survival rates as a result of cadmium exposure. In order to demonstrate functional role of two ATP binding sites in cadmium defense, we have transformed both wild type (SP1) and hyperactivated ras mutant (IR2.5) strains with several plasmids differing in the presence of ATP binding sites. When an extra copy of functional hsp104 gene with both ATP binding sites was overexpressed with GPD-promoter, cells showed increased survival rate against cadmium stress than mutants with ATP binding sites changed. The degree of protection in the presence of two ATP binding sites was similarly observed in ira2-deleted hyperactivated ras mutant, which was more sensitive to oxidative stress than wild type cell. We have concluded that the greater sensitivity to cadmium stress in the absence of two ATP binding sites is attributed to the higher concentration of reactive oxygen species (ROS) produced by cadmium exposure based on the fluorescence tests. These findings, taken all together, imply that the mechanism by which cadmium put forth toxic effects may be closely associated with the oxidative stress, which is regulated independently of the Ras-cAMP pathway. Our study provides a better understanding of cadmium defense itself and cross-talks between oxidative stress and metal stress, which can be applied to control human diseases due to similar toxic environments.
The sodium cooled fast reactor (SFR) is one of the Gen-IV reactors with the most operating experience accumulated. Although the technology level is the most mature among the Gen-IV reactors, there is still a safety problem that has not been solved, which is the sodium-water reaction. Since sodium and water are separated only by a heat transfer tube with a thickness of only a few mm, there is inherently a risk of a sodium-water reaction (SWR) accident in the SFR. In this study, it is attempted to quantitatively evaluate the resistance of SWR accidents by replacing the shell and tube steam generator with printed circuit steam generator (PCSG) as a method to mitigate the SWR accident. To do this, a CATS-S (Compact Accident Tolerance Steam Generator-SWR) facility was designed and built. And for the quantitative evaluation of accident resistance, a methodology for measuring the impingement wastage rate was established. As a result of this research, the impingement wastage rate caused by SWR generated in a PCSG was measured first time. It was confirmed that the impingement wastage phenomenon was suppressed in the PCSG, and the accident resistance was higher than that of the SWR through comparison with the experimental results performed in the existing shell and tube steam generator. In conclusion, a PCSG is more resistant to impingement wastage as a result of the SWR accident than existing shell and tube steam generators, and it is estimated that a PCSG can mitigate SWR accidents, an inherent problem of SFR.
Geon Hui Lee;Jae Hyun Park;Kyung Jin Jung;Sung-Man Kang;Seung-Kyu Choi;Jeong Min Woo
Journal of Wind Energy
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v.14
no.4
/
pp.50-56
/
2023
With the abnormal weather phenomena caused by global warming, the frequency and intensity of lightning strikes are increasing, and lightning accidents are becoming one of the biggest causes of failures and accidents in offshore wind turbines. In order to secure generator operation reliability, effective and practical measures are needed to reduce lightning damage. Because offshore wind turbines are tall structures installed at sea, the possibility of direct lightning strikes is very high compared to other structures, and the role of surge protection devices to minimize damage to the electrical and electronic circuits inside the wind turbine is very important. In this study, a varistor, which is a key element for a class 1 surge protection device for direct lightning protection, was developed. The current density was improved by changing the varistor composition, and the distance between the electrode located on the varistor surface and the edge of the varistor was optimized through a simulation program to improve the fabrication process. Considering the combined effects of heat distribution, electric field distribution, and current density on the optimized varistor surface, silver electrodes were formed with a gap of 0.5 mm. The varistor developed in this study was confirmed to have an energy tolerance of 10/350 ㎲, 50kA, which is a representative direct lightning current waveform, and good protection characteristics with a limiting voltage of 2 kV or less.
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