• Title/Summary/Keyword: heat tolerance

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Monthly Changes of Skin Temperature in Koreans by Sexes and Ages (성별, 연령별로 본 한국인의 월별 피부온)

  • 김명주;최정화
    • Journal of the Korean Society of Clothing and Textiles
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    • v.21 no.2
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    • pp.314-324
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    • 1997
  • The purpose of this study was to evaluate the thermoregulatory response level on the heat and cold tolerance with investigating monthly changes of skin temperature in Koreans and to obtain the basal information for standard amount of clothing weight, indoor climate and working condition. Forty eight subjects in 5 age groups (6~11, 12~19, 20~44, 45~64, 65~76 years old) with both sexs were taken from Seoul and Kyunggi probince. All subjects were measured skin temperature, mean skin temperature, Total clothing weight in neutral condition in each month from June 1994 to May 1995. The results obtained are summarized as follows: 1. Skin temperature of all subjects was low on February, March and April, and was high on June, July and August. Temperatures of the torso (forehead and abdomen) and the lower limbs (leg and foot) were remarkably different. In general, most of skin temperatures except of hand, thigh and foot were higher in males. 2. Mean skin temperature was 0.5'c higher in males than female with ranging 32.5~34.5$^{\circ}C$ in males and 32.1~34.1$^{\circ}C$ in females. Also, mean skin temperature of 6~11 age group were higher than that of 45~ 64 age group in both sexs.

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Arsenic-Induced Differentially Expressed Genes Identified in Medicago sativa L. roots

  • Rahman, Md. Atikur;Lee, Sang-Hoon;Kim, Ki-Yong;Park, Hyung Soo;Hwang, Tae Young;Choi, Gi Jun;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.3
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    • pp.243-247
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    • 2016
  • Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

Clinical Result of Combined Radiotherapy and Hyperthermia Induced by 915 MHz Microwave and Ultrasound in Locally Advanced Malignant Tumors of Head and Neck (915 MHz 극초단파 및 초음파를 이용한 온열치료와 방사선치료 병합치료에 의한 두경부암의 치료성적)

  • Koh Kyoung-Hwan;Park Young-Hwan;Cho Chul-Koo;Yoo Seong-Yul
    • Korean Journal of Head & Neck Oncology
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    • v.6 no.1
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    • pp.40-45
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    • 1990
  • Thirty five lesions of 35 patients with locally advanced malignant tumors of head and neck were received thermoradiotherapy with ultrasound and/or 915 MHz microwave. Most of all patients were failed with previous conventional therapeutic trial. Hyperthermia had been done immediately after radiotherapy, twice a week, $43^{\circ}C$ for one hour and radiotherapy had been done 5 fractions per week with a fraction size of 2 Gy up to total 30 to 60 Gy. Conclusions are as follows; 1) Total response rate (CR+PR) of thermoradiotherapy with microwave and ultrasound was 80%. 2) Tumor depth, minimum temperature of tumor center, number of heat fraction and irradiation dose were statistically significant factors affecting response. 3) Hyperthermia with microwave and ultrasound can be used efficiently to control locally advanced malignant tumors in head and neck whether previously received near tolerance dose of radiotherapy or not.

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A Safety Evaluation of Genetically Modified Feedstuffs for Livestock Production; the Fate of Transgenic DNA and Proteins

  • Beever, D.E.;Glenn, K.;Phipps, R.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.5
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    • pp.764-772
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    • 2003
  • Two genetic constructs used to confer improved agronomic characteristics, namely herbicide tolerance (HT) in maize and soyabean and insect resistance (Bt) in maize, are considered in respect of feeding to farm livestock, animal performance and the nutritional value and safety of animal products. A review of nucleic acid (DNA) and protein digestion in farm livestock concludes that the frequency of intact transgenic DNA and proteins of GM and non-GM crops being absorbed is minimal/non existent, although there is some evidence of the presence of short fragments of rubisco DNA of non-GM soya in animal tissues. It has been established that feed processing (especially heat) prior to feeding causes significant disruption of plant DNA. Studies with ruminant and non-ruminant farm livestock offered GM feeds demonstrated that animal performance and product composition are unaffected and that there is no evidence of transgenic DNA or proteins of current GM in the products of animals consuming such feeds. On this evidence, current HT and Bt constructs represent no threat to the health of animals, or humans consuming the products of such animals. However as new GM constructs become available it will be necessary to subject these to rigorous evaluation.

Genomic Organization of Heat Shock Protein Genes of Silkworm Bombyx mori

  • Velu, Dhanikachalam;Ponnuvel, Kangayam M.;Qadri, Sayed M. Hussaini
    • International Journal of Industrial Entomology and Biomaterials
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    • v.15 no.2
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    • pp.123-130
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    • 2007
  • The Hsp 20.8 and Hsp 90 cDNA sequence retrieved from NCBI database and consists of 764 bp and 2582 bp lengths respectively. The corresponding cDNA homologus sequences were BLAST searched in Bombyx mori genomic DNA database and two genomic contigs viz., BAAB01120347 and AADK01011786 showed maximum homology. In B. mori Hsp 20.8 and Hsp 90 is encoded by single gene without intron. Specific primers were used to amplify the Hsp 20.8 gene and Hsp 90 variable region from genomic DNA by using the PCR. Obtained products were 216 bp in Hsp 20.8 and 437 bp in Hsp 90. There was no variation found in the six silkworm races PCR products size of contrasting response to thermal tolerance. The comparison of the sequenced nucleotide variations through multiple sequence alignment analysis of Hsp 90 variable region products of three races not showed any differences respect to their thermotolerance and formed the clusters among the voltinism. The comparison of aminoacid sequences of B. mori Hsps with dipteran and other insect taxa revealed high percentage of identity growing with phylogenetic relatedness of species. The conserved domains of B. mori Hsps predicted, in which the Hsp 20.8 possesses ${\alpha}-crystallin$ domain and Hsp 90 holds HATPase and Hsp 90 domains.

Development of Multi Forming Product Progressive Die for STS 304 Marine Part Sheet Metal (Part 2)

  • Sim, Sung-Bo;Sung, Yul-Min;Song, Young-Seok
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2000.04a
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    • pp.151-156
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    • 2000
  • Ultra precision progressive die have used for above one million's lot size of production part. In the field of design and making tool for press working, the progressive die for sheet metal (STS 304, thickness : 0.5mm) is a specific division. In order to prevent the defects, the optimum design of the production part, strip layout, die design, die making and tryout etc. are necessary. They require analysis of many kinds of important factors, i.e. theory and practice of metal press working and its phenomena, die structure, machining condition for die making, die materials, heat treatment of die component, know-how and so on. In this study, we designed and constructed a progressive die of multi-stage and performed try out. Out of these processes the die development could be taken for advance. Especially the result of tryout and its analysis become the characteristics of this paper (part 1 and part 2) that nothing might be ever seen before such as this type of research method on all the processes. In the part 2 of this study we treated die making and tryout mostly.

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Conformational Lock and Dissociative Thermal Inactivation of Lentil Seedling Amine Oxidase

  • Moosavi-Nejad, S. Zahra;Moosavi-Movahedi, Ali-Akbar;Rezaei-Tavirani, Mostafa;Floris, Giovanni;Medda, Rosaria
    • BMB Reports
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    • v.36 no.2
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    • pp.167-172
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    • 2003
  • The kinetics of thermal inactivation of copper-containing amine oxidase from lentil seedlings were studied in a 100 mM potassium phosphate buffer, pH 7, using putrescine as the substrate. The temperature range was between $47-60^{\circ}C$. The thermal inactivation curves were not linear at 52 and $57^{\circ}C$; three linear phases were shown. The first phase gave some information about the number of dimeric forms of the enzyme that were induced by the higher temperatures using the "conformational lock" pertaining theory to oligomeric enzyme. The "conformational lock" caused two additional dimeric forms of the enzyme when the temperature increased to $57^{\circ}C$. The second and third phases were interpreted according to a dissociative thermal inactivation model. These phases showed that lentil amine oxidase was reversibly-dissociated before the irreversible thermal inactivation. Although lentil amine oxidase is not a thermostable enzyme, its dimeric structure can form "conformational lock," conferring a structural tolerance to the enzyme against heat stress.

Quantifying rice spikelet sterility on Vietnamese cultivars (Oryza sativa L.) under high temperature and shading condition

  • Tran, Loc Thuy;Shaitoh, Kuniyuki
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.43-43
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    • 2017
  • During grain filling period, rice is affected by many environmental factors; including temperature, water, radiation and soil nutrition condition. In future climate, greater shading and heat tolerance will be required in rice. In this study, the effect of shading and high temperature on spikelet sterility was conducted on fourteen Vietnamese cultivars. Field experiments were studied in 2015 and 2016 to evaluate the response of Vietnamese cultivars under high temperature during grain filling stage. The high temperature and shading were applied by closing two sides of growth chamber and covered by a black cloth (50% reduced solar radiation) under the field condition after the first cultivar heading. The sterility increased significantly under high temperature and shading. The highest percentage sterile spiketlets was observed in 'Jasmine 85' (71.7%) under shading and in 'OM4900' (53.4%) under high temperature in 2015 and 2016, respectively. Among the treatments, the percentage of sterile spekelets in Vietnamese cultivars under shading was highest which was 54.9% and 41.8% in 2015 and 2016, respectively. Yield components reduced significantly in both of shading and high temperature. Corresponding with significantly decrease in yield components, the yield in high temperature and shading decreased strongly in both 2015 and 2016.

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Heterogeneous overexpression of Oryza sativa salt induced RING Finger protein OsSIRF1 positively regulates salt and osmotic stress in transgenic Arabidopsis

  • Chapagain, Sandeep;Jang, Cheol Seong
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.150-150
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    • 2017
  • Plants suffer from various abiotic stresses among them; soil salinity is one of major adverse factor in declining agricultural productivity. So, development of salt stress tolerance crops have potential role to increase crop production. The RING finger proteins are known to play crucial roles in abiotic stress environment to plants. In this study, we identified one Salt-responsive Really${\underline{I}nteresting}$ ${\underline{n}ew}$ ${\underline{g}ene}$ (RING) E3 ubiquitin ligase gene OsSIRF1 from rice root tissues during salt stress and studied its molecular function. Expression of OsSIRF1 was induced under various abiotic stress conditions, including salt, heat, drought, and ABA. Result of an in vitro ubiquitination assay clearly showed that OsSIRF1 Possess an E3 ligase activity. Moreover, OsSIRF1 was found to be localized to the nucleus within the cell. Heterogeneous overexpression of OsSIRF1 in Arabidopsis improved seed germination and increased root length under salt and Manitol stress conditions. Taking together, these results suggested that OsSIRF1 may be associated with plant responses to abiotic stressors and positively regulates salt and osmotic stress environment.

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An Experimental Study on the Effect of Repeated Hyperthermia on the Radiation Injury (반복 온열료법이 방사선조사효과에 미치는 영향에 관한 실험적 연구)

  • Choi, Young-Hi;Park, Charn-Il;Han, Man-Chung
    • Radiation Oncology Journal
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    • v.2 no.1
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    • pp.1-9
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    • 1984
  • To evaluate the influence of prior heat treatment on the thermal enhancement of irradiation effect after hyperthermia, an experimental study was carried out using a total of 80 mice. Hyperthermia was carried out at $43^{\circ}C$ for 40 minutes and was repeated with various intervals. A single dose of 3,000 rad was delivered on skin of mouse tail immediately after the second hyperthermia. The skin changes of the irradiated mouse tail were observed from 7th to 35th post-irradiation days, and the skin scores were analyzed. The results are as follows, 1. The radiation damage on mouse skin increased significantly when radiation was combined with hyperthermia. 2. The radiation damage after repeated hyperthermia is significantly less than that after single hyperthermia, when the interval is 1 to 6 days. 3. As a result, thermal tolerance persists from 1 through 6 days after the initial hyperthermia.

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