• KSBB Journal
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• v.27 no.4
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• pp.257-262
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• 2012

The gene encoding Thermus thermophilus HJ6 laccase (Tt-laccase) was cloned, sequenced, and comprised of 1,389 nucleotides encoding a protein (462 amino acids) with a predicted molecular mass of 51,049 Da. The deduced amino acid sequence of Tt-laccase showed 99.7% and 44.3% identities to the Thermus thermophilus HB27 laccase and Synechococcus sp. RS9917 laccase, respectively. Tt-laccase gene was expressed as a fusion protein with six histidine residues in E. coli Rosetta-gami (DE3) cells, and the recombinant protein was purified to homogeneity. UV-Vis spectrum analysis revealed that the enzyme has copper atoms, a type I Cu(II) and a type III binuclear Cu(II). The optimum pH for the oxidation of guaiacol was 5.0 and the optimum temperature was $90^{\circ}C$ The half-life of heat inactivation was about 120 min at $90^{\circ}C$ The enzyme reaction was inhibited by sodium azide, L-cystein, EDTA, dithiothreitol, tropolone, and kojic acid. The enzyme oxidized various known laccase substrates, its lowest $K_m$ value being for 4-hydroxyindole, highest $k_{cat}$ value for syringaldazine, and highest $k_{cat}/K_m$ for guaiacol.

• Journal of Korea Foundry Society
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• v.37 no.1
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• pp.1-13
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• 2017

The purpose of this study is to increase the part recovery rate (to more than 70%) during the casting of a ductile cast iron turbo charger housing using a heater around the riser. Before creating a casting mold, various runner and riser systems were designed and analyzed with a casting simulation analysis tool. The design variables were the heater temperature, top insulation, riser location, riser diameter and the riser shape. During the feeding from the riser to the part, the reverse model was better than the forward model. When heating the riser (above $600^{\circ}C$), solidification of the riser was delayed and the feeding effect was suitable compared to that without heating. At a higher heating temperature, less solidification shrinkage and porosity were noted inside the part. On the basis of a casting simulation, eight molds were fabricated and casting experiments were conducted. According to the experimental conditions, external and internal defects were analyzed and mechanical properties were tested. The ultimate tensile strength and elongation outcome were correspondingly more than 540MPa and 5% after a heat treatment. In addition, a maximum part recovery rate of 86% was achieved in this study.

• Polymer(Korea)
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• v.38 no.4
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• pp.457-463
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• 2014

In this study, the changes in the chemical structure and the physical property of fluoropolymer films (PTFE, FEP, PFA, PVDF, and ETFE) induced by $Co^{60}$ gamma ray in air, $N_2$, and vacuum environments were investigated. FTIR spectra of the irradiated fluoropolymers indicate that the oxidation proceeded by the reaction of radicals generated by irradiation with oxygen in air. The changes in the heat of fusion and the degree of crystallinity of the irradiated fluoropolymers were investigated using DSC and the results indicate that the scission and crosslinking reactions of the irradiated fluoropolymers were largely influenced by the chemical structure. It was also found that the mechanical property of the irradiated fluoropolymer films under an air atmosphere was significantly decreased.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.4
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• pp.413-421
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• 2010

In this study, residual-stress distributions in welds with different strength used in natural gas pipelines are calculated by using finite-element analysis and simulating a realistic welding process. The temperature and residual-stress analysis results are compared with the real fusion profile and the application results of the Fitness-For-Service assessment code, API 579 in order to validate the finite-element analysis model and procedure. Parametric study is performed to assess the effect of welding and material variables such as mechanical strength mismatch, the strength of weld metal, reinforcement, and heat input on the residual stress distributions. Finally, on the basis of the parametric study results, the effects of these variables on residual stress distributions are investigated. In particular, the strength mismatch between base metals has an insignificant effect on residual-stress distributions.

• Journal of Sensor Science and Technology
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• v.9 no.1
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• pp.44-50
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• 2000

Every object emits some energy from its surface. The emission energy forms surface heat distribution which we can capture by using an infrared thermal imager. The infrared thermal image may include valuable information regarding to the subsurface anomaly of the object. Since a thermal image reflects surface clutter and subsurface anomaly, we have difficulty in extracting the information on the subsurface anomaly only with thermal images taken under a wavelength. Thus, we use visible wavelength images of the object surface to remove exterior clutter. We, therefore in this paper, visualize the infrared image for overlaying it with a visible wavelength image. First, we make an interpolated image from two ordinary images taken from both sides of an infrared sensor. Next, we overlay the intermediate image with an infrared image taken from the infrared camera. The technique suggested in this paper can be utilized for analyzing the infrared images on non-destructive inspection against disaster and for safety.

• Progress in Superconductivity and Cryogenics
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• v.24 no.2
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• pp.7-18
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• 2022

European efforts to design superconducting conductors for a future tokamak have involved Nb₃Sn cable-in-conduit conductor (CICC). Nb₃Sn coils which undergo heat treatment to activate the Nb₃Sn material are mostly produced through the wind-then-react route. However, some Nb₃Sn coils have been proposed with CICCs of the react-then-wind route. The latter CICCs are physically constrained due to handling limitations which if not adhered to will result in irrecoverable damage to the Nb₃Sn cable inside, nullifying any performance advantage. A group at the Swiss Plasma Center has proposed such CICC designs, constructing samples and testing them for performance. The characteristics and performance of these react & wind (R&W) CICCs are compared with the more common wind & react (W&R) CICCs, and it is found that the R&W designs show more extreme characteristics than typical W&R Nb₃Sn CICCs for some parameters that are known to influence CICC performance. Where the R&W CICCs extend the range of those parameters, they also continue trends formed by the W&R CICCs with the parameters. The main observation, however, is that although the current sharing temperature performances of the R&W samples are above the average of the W&R samples they were compared to, they are not the highest. A similar observation applies to a cost comparison of the superconducting material where the R&W CICCs are found to be relatively cheap but not the cheapest. Given these results, clear practical advantages to the R&W CICC design is not evident.

• Proceedings of the KSME Conference
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• 2001.11a
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• pp.180-185
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• 2001

In the life assessment for plant structural component, the research on deterioration of toughness and material properties occurred in weldments has been considered as very important problems. In general, the microstructures composed in weldments are hugely classified with weld metal(W.M), fusion line(F.L), heat affected zone(HAZ), and base metal(B.M). It has been reported that the creep characteristics on weldments having variable microstructures could be unpredictably changed. Furthermore, it is also known that HAZ adjacent to F.L exhibits the decreased creep strength compared to those in base or weld metals, and promotes the occurrence of Type III and Type IV cracking due to the growth of grains and the coarsening carbides precipitated in ferritic matrix by welding and PWHT processes. However, the lots of works reported up to date on creep damage in power plant components have been mostly conducted on B.M and the creep properties on a localized microstructures in weldments have not as yet been throughly investigated. In this paper, for various microstructures such as coarse grain HAZ(CGHAZ), W.M and B.M in X20CrMoV121 steel weldment, the small punch-creep(SP-Creep) test using miniaturized specimen(t=0.5mm, 0.25mm) is performed to investigate a possibility for creep characteristics evaluation.

• Elastomers and Composites
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• v.45 no.1
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• pp.32-39
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• 2010

The mechanical properties of PTFE 100%, PTFT 90% + carbon black 10%, PTFE 85% + glass fiber 15%, PTFE 80% + glass fiber 15% + molybdenum disulfide ($MoS_2$) 5%, PTFE 75% + glass fiber 25%, and PTFE 75% + carbon black 18% + graphite 7% composites were investigated in this study. The differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) were used to examine the heat of fusion(${\Delta}H_f$) and thermal stability of the composites. Also, the wear surface and wear volume of PTFE lip seal were examined using the durability test. Wear surface was observed using scanning electron microscope (SEM). It was found that the hardness, wear resistance and durability were enhanced by adding glass fiber and molybdenum disulfide into pure PTFE, but tensile strength and elongation were decreased. According to the experimental results, the composite (PTFE + 15% glass fiber + 5% molybdenum disulfide) showed the best properties for applying to oil-seal among six types of PTFE composites.

• Journal of Energy Engineering
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• v.12 no.1
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• pp.1-10
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• 2003

Pressurized fluidized bed combustion unit is operated at pressures of 1~1.5 MPa with combustion temperatures of 850~87$0^{\circ}C$. The pressurized coal combustion system heats steam, in conventional heat transfer tubing, and produces a hot gas supplied to a gas turbine. Gas cleaning is a vital aspect of the system, as is the ability of the turbine to cope with some residual solids. The need to pressurize the feed coal, limestone and combustion air, and to depressurize the flue gases and the ash removal system introduces some significant operating complications. The proportion of power coming from the steam : gas turbines is approximately 80:20%. Pressurized fluidized bed combustion and generation by the combined cycle route involves unique control considerations, as the combustor and gas turbine have to be properly matched through the whole operating range. The gas turbines are rather special, in that the maximum gas temperature available from the FBC is limited by ash fusion characteristics. As no ash softening should take place, the maximum gas temperature is around 90$0^{\circ}C$. As a result a high pressure ratio gas turbine with compression intercooling is used. This is to offset the effects of the relatively low temperature at the turbine inlet.

• Fire Science and Engineering
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• v.34 no.2
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• pp.14-21
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• 2020

This paper presents the study of a fire risk to the backside of two miniatures of ISO 9705 2/5 using a lightweight partition for indoor space division and reproduction of the ISO 9705 test. An SGP partition, stud partition, glass wool panel, urethane foam panel, sandwich panel, and glass partition were selected as the test specimens, which are frequently used in construction. According to the ISO 9705 test standard, stabilization was achieved using a measuring device that recorded data before the ignition of a burner and continued recording for 120 s thereafter. After ignition was achieved, the power was increased to 300 kW for 600 s and then reduced to 100 kW for 600 s. The specimens were subsequently observed for 180 s, and the fire risk to the backside and the fire pattern of the wall unit were analyzed. Owing to the amount of heat generated by the ignition source, the maximum temperature of the backside was observed to be 67.7 ℃ for the SGP partition, 55.1 ℃ for the stud partition, 52.4 ℃ for the glass wool panel, 727.4 ℃ for the sandwich panel, 561 ℃ for the urethane foam panel, and 630.5 ℃ for the glass partition. In the cases of the sandwich and urethane foam panels, the explosion of flammable gas occurred by virtue of fusion of the interior materials. The reinforced glass was fractured owing to the temperature difference between the heat- and nonheat-responsive parts. Ultimately, the fire risk to the nearby section room was deemed to be high.