• Title/Summary/Keyword: hatchery

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Vertebral abnormality in Hatchery-reared River puffer, Takifugu obscurus fingerlings (인공종묘 생산 황복 (Takifugu obscurus) 치어의 척추 변형에 관하여)

  • Park, Sung-Woo;Kang, Kook-Hee
    • Journal of fish pathology
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    • v.20 no.2
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    • pp.129-137
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    • 2007
  • Skeletal deformity in hatchery-reared river puffer (Takifugu obscurus) fingerlings occurred. The vertebral abnormality was recognized with naked eyes 80 days after hatching. Affected fish had a good appetite but no clinical signs were found except the vertebral abnormality. As more than 90% of the hatchery-reared fish exhibited vertebral abnormality, the fingerlings could not be used for commercial seeds any more. Morphological changes in the vertebrate and the swim bladder were observed with a soft X-ray. Histopathological changes on the caudal muscle around the deformed vertebrae, gill, and spleen were also examined to clarify the cause of the deformity. Spinal curvature occurred between vertebrae 10 and 12, but any malfunction of the swim bladder was not found. The diameters of the muscle fibers around the deformed vertebrae were much smaller and more irregular than those in normal fish, and the gabs between the fiber bundles were greatly enlarged. No evidence of inflammation response was found in the muscle layer. In the hatchery, feed was putting at the position of water inflow, which might attract the fingerings to move toward the feed in spite of incomplete development of their caudal muscle fibers. From these results, it is suggested that the high speed of water current in rearing aquaria might be associated with the development of vertebral abnormality.

Optimal Method for Fertilization of Surf Clam, Spisula sachalinensis (북방대합, Spisula sachalinensis 수정을 위한 최적방법)

  • Lee Jeong Yong;Chang Yun Jeong;Chun Min Jee;Chang Hae Jin;Chang Young Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.2
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    • pp.135-139
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    • 2002
  • In order to obtain the basic information for the seedling production of surf clam, Spisula sachalinensis, sperm motility and optimal method for fertilization were investigated. Sperm concentration of S. sachalinensis milt was$ 2.02{\times}10^{10}\;cell/mL$ and approximately $96.0\%$ of sperm showed forward movement after exposure to seawater. When sperm and eggs obtained by incision method were fertilized in 1 hour and 4 hours, respectively, high fertilization and hatching rate were achieved. The optimal sperm concentrations and egg density for fertilization and hatching were 10$\~$100 inds./egg and 100$\~$200 inds./mL sea water, respectively.

Gametogenic Cycle and Fine Structure of Ripe Germ Cells in the Pacific Oyster, Crassostrea gigas on the South Coast of Korea

  • Choi Youn Hee;Kim Tae Ik;Hur Young Baek;Go Chang-Soon;Chang Young Jin
    • Fisheries and Aquatic Sciences
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    • v.6 no.2
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    • pp.51-58
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    • 2003
  • The gonadal development and the gametogenic cycle and the fine structure of ripe germ cells of the cultured Pacific oyster, Crassostrea gigas were investigated using oysters monthly collected from the southern coast of Korea from October 2000 to September 2001. Monthly changes in the condition index were similar to that of meat weight rate and the highest value was observed in between April and May, and the lowest value in August. The external colors of the testis and the ovary were milky white and yellowish, respectively. The spawning period of the Pacific oyster was continued from May to September, with a peak in July. The gametogenic cycle could be classified into five successive stages: multiplicative stage (December to March), growing stage (March and April), mature stage (April to June), spawning stage (June to August) and resting stage (August to January). Variety of egg yolk granules, lipid granules, mitochondria, and endoplasmic reticula were observed in cytoplasm of ripe oocyte. The spermatozoon consisted of the head, middle piece and tail; including cap-shaped acrosome with domed structure, elliptical shaped nucleus, four mitochondria, two centrioles and flagellum.

A Report on the Mass Mortality of the Farmed Japanese Scallop, Patinopecten yessoensis on the Korean Coasts of the East Sea

  • Jo, Q-Tae;Kim, Su-Kyoung;Lee, Chu;Rahman, Mohammad M.;Lee, Chae-Sung;Oh, Bong-Se
    • The Korean Journal of Malacology
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    • v.25 no.2
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    • pp.93-96
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    • 2009
  • Unexpected mass mortality has been one of the drawbacks in front of the stable production of Japanese scallop (Patinopecten yessoensis) on the Gangwon coasts of the East Sea. The preliminary data from our routine observation revealed that the mortality appeared to be related to variation of water temperature in the farming site and the degree of the mortality was dependent on scallop strain. The present study performed to verify the preliminary findings exhibited that the mortality was closely related to daily temperature variation rather than monthly variation. Daily temperature variation was particularly damageable to the scallop during the temperature elevation period. Scallops from hatchery seeds (Chinese strain) were more tolerant against the temperature variation over those from wild seeds. The hatchery scallop gain of the temperature tolerance was probably due to their larval experience to higher temperature in the hatchery as well as their maternal genetic acclimation to upper temperature extreme of the Chinese environment which was recently found.

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Vertebral abnormality in Hatchery-reared Black Sea Bream (Acanthoparus schlegelii) fingerlings (인공종묘 생산 감성돔 (Acanthoparus schlegelii) 치어의 척추 변형에 관하여)

  • Park, Sung-Woo;No, Yun-San;Yu, Jin-Ha;Kim, Jin-Do
    • Journal of fish pathology
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    • v.21 no.2
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    • pp.139-148
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    • 2008
  • Vertebral deformity in hatchery-reared black sea bream (Acanthoparus schlegelii) fingerlings occurred. Deformed fish had a good appetite but no clinical signs were found except the vertebral abnormality and darkness around the dorsal skin of the deformed vertebra. As more than 90% of the hatchery-reared fish exhibited vertebral abnormality, the fingerlings could not be used for commercial seeds any more. No morphological change in the swim bladder was observed. Histopathological changes on the deformed vertebra, gill, liver, and spleen were observed to clarify the cause of the deformity. The vertebra were irregularly deformed upward with the shape of "V" in an alphabetic character causing the spinal cord and dorsal aorta suppressed. The diameters of the muscle fibers around the deformed vertebra, especially, in the dorsal part, were much smaller and more irregular than those in normal fish, and the gabs between the fiber bundles were enlarged. No evidence of inflammatory responses in the lateral musculature were found. On the basis of normal inflation of the swim bladder, cultural environments for growth of the fingerlings, and histopathological alterations in the muscle, vertebra and gills, it is suggested that high speed of water current in the culturing aquaria between 23 to 30 days after hatching was responsible for the development of vertebral abnormality.