Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Optimal Method for Fertilization of Surf Clam, Spisula sachalinensis

북방대합, Spisula sachalinensis 수정을 위한 최적방법

  • 이정용 (부경대학교 양식학과) ;
  • 장윤정 (국립수산진흥원 강릉수산종묘시험장) ;
  • 전민지 (국립수산진흥원 강릉수산종묘시험장) ;
  • 장해진 (국립수산진흥원 강릉수산종묘시험장) ;
  • 장영진 (국립수산진흥원 강릉수산종묘시험장)
  • Published : 2002.03.01
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Abstract

In order to obtain the basic information for the seedling production of surf clam, Spisula sachalinensis, sperm motility and optimal method for fertilization were investigated. Sperm concentration of S. sachalinensis milt was$ 2.02{\times}10^{10}\;cell/mL$ and approximately $96.0\%$ of sperm showed forward movement after exposure to seawater. When sperm and eggs obtained by incision method were fertilized in 1 hour and 4 hours, respectively, high fertilization and hatching rate were achieved. The optimal sperm concentrations and egg density for fertilization and hatching were 10$\~$100 inds./egg and 100$\~$200 inds./mL sea water, respectively.

이 연구에서 북방대합의 정자 운동성 및 수정률과 부화율 향상을 위한 수정방법을 조사하였다. 북방대합 원정액의 정자 농도는 $2.02{\times}10^{10}/mL$였으며, 채정 직후에는 원정액이나 해수중의 정액에서 모두 $96.0\%$의 정자가 전진운동을 하였다. 절개법으로 얻어진 알과 정자는 각각 채란 4시간 이내에, 채정 1시간 이내에 수정하는 것이 효과적이었다. 인공수정을 위한 알 1개에 대한 정자수는 10$\~$100개에서 수정률과 부화율이 모두 높았으며, 수정 후 1시간 이내에 세란하는 것이 효과적이었다. 최적의 수정 및 발생을 위한 알의 수용밀도는 100$\~$200 개/mL였다.

Keywords

References

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