• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.5
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• pp.856-862
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• 1994

In salt-preserved foods of every kinds, it was examined the growth condition of halophilic bacteria that induced a change of colour, taste, nutritive substance, a production condition of enzyme and a character of crude enzyme. Used bacteria is H. halobium a kind of extremely halophilic bacteria, and the required of optimum culture needed a quite long time of crude enzyme production is 168 hours. Optimum pH is about 7-7.5, so the traditional food of such neutrality pH as soybean paste and soy sauce particularly come into trouble because the growth can flourish in neutrality or alkaliescence, and the crude enzyme also appeared that best activation between pH 6 and pH 8. The optimum temperature is about 37$^{\circ}C$, the optimum temperature of enzyme is about 40 $^{\circ}C$ and the temperature stability is settled for 15 minutes and it is completely inactivated at 10 minutes. In the influence of each metal ion, Fe++ and Mn++ a stimulated the growth of H.halobium and the activation of enzyme, Cu++ and Zn++ were identified that made the growth and the activation of enzyme inhibit.

• Journal of the Korean Applied Science and Technology
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• v.35 no.1
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• pp.157-162
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• 2018

The partial molar volumes of an antidepressant in Halobacteriun Halobium and in suspensions of several lipids have been determined at $25^{\circ}C$ it using a excess volume dilatometer. The potency of general antidepressant, Fluoxetine has long been known to correlate with lipid solubility. Denaturations of the vesicle, which is a sole membrane protein in the purple membrane of Halobacteriun Halobium, were studied by absorption changes at 280 nm and fluorescence changes at 330 nm with excess volume dilatometer. The 1H NMR analysis of viscous polymer solutions by diffusion interchange is the important step by measurement. The partial molar volume and particle size of Fluoxetine in Halobacteriun Halobium were measured to be positive. An antidepressant can prevent diseases that produce a variety of cognitive and mental symptoms based on low morale and depression, resulting in poor daily performance.

• KSBB Journal
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• v.13 no.3
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• pp.268-271
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• 1998

Bacteriorhodopsin in the purple membrane (PM) of halobacteria has recently been attracting much attention to be used as a component of molecular electron device and optical computers. In order to increase the productivity of bacteriorhodopsin in high cell density cultures of Halobacterium halobium R1, an internal membrane cell-retention bioreactor system was employed. As a result, the production of cell mass at OD660 of 12 and of bacteriorhodopsin at 125-130 mg/L were obtained using the internal membrane bioreactor system at a dilution rate of 0.066 hr-1. The productivity achieved by the internal membrane system (0.7 mg/L$.$hr) was 3.5-fold higher than that obtained by the corresponding batch cultivations (0.2 mg/L$.$hr).

• Journal of the Korean Applied Science and Technology
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• v.13 no.3
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• pp.119-125
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• 1996

The excess volumes of mixing of benzyl alcohol, ethyl alcohol, halothane, and procaine in vesicle and suspensions of several lipids have been determined at $25^{\circ}C$ using a excess volume dilatometer. The potency of general anesthetics has long been known to be correlated with lipid solubility. Denaturations of the bacteriorhodopsin, which is a sole membrane protein in the purple membrane of Halobacteriun Halobium, were studied by UV/Vis absorption changes. The excess volumes of mixing of benzyl alcohol and procaine in egg lecithin were all found to be negative and this result was confirmed as Miller's supposition.

• Journal of the Korean Applied Science and Technology
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• v.28 no.2
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• pp.247-250
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• 2011

The excess volumes of mixing of benzyl alcohol and halothane in vesicle and in suspensions of several lipids have been determined at $25^{\circ}C$ it using a excess volume dilatometer. The potency of general anesthetics has long been known to correlate with lipid solubility. Denaturations of the vesicle, which is a sole membrane protein in the purple membrane of Halobacteriun Halobium, were studied by absorption changes at 280 nm and fluorescence changes at 330 nm and excess volume dilatometer. The particle size analysis of viscous polymer solutions by diffusional interchange is the key step by measurement. The excess volume of mixing in chitosan was found to be negative, whereas them of purple membrane, Halobacteriun Halobium and red membrane were positive in benzyl alcohol and halothane. This result was confirmed as Miller's supposition.

• Proceedings of the Optical Society of Korea Conference
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• 2000.02a
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• pp.114-115
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• 2000

Halobacterium halobium에서 추출한 Bacteriorhodopsin(bR)을 고분자 물질에 첨가해 박막화하였다. 이 단백질 박막 소자의 photodynamic특성을 이용하여 광 switching system을 구성하였다. 광 switch system은 pump광을 이용해 B상태와 M상태에서 축퇴4광파 혼합방식으로 구성했다. Red, blue, violet 광원을 사용하여 B상태와 M상태 격자를 형성하고 on-off되는 위상공액신호를 측정했다. (중략)

• Journal of the Korean Chemical Society
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• v.23 no.5
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• pp.320-324
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• 1979

Purple membrane from Halobacterium halobium was incorporated into 7.5% polyacrylamide gels. Absorption and circular dichroic spectra of purple membrane incorporated with gels were obtained at various pH. The spectra of these gels measured at pH 7.0 were essentially identical with those obtained in the aqueous suspension of purple membrane. Acid titration of the gels showed the transition to a form absorbing at 605nm $(bR_{605}^{acid}$) at pH 2.6, and to a second form at 565nm $(bR_{565}^{acid})$ at pH 0.8. Dark-adapted gels showed an isosbestic point for each transition whereas light-adapted gels did not. Visible CD spectra of $bR_{570}^{LA},\;bR_{305}^{acid}\;and\;bR_{565}^{acid}$ all showed the typical bilobed pattern. CD spectra measured at UV wavelength region were also independent of the variation of pH in terms of molar ellipticity and spectral shape. The protonated species $bR_{605}^{acid}$ may be one of the intermediates formed during the normal photochemical cycle of purple membrane. Most probably, the species $bR_{605}^{acid}$ is considered to be $O^{640}$ in the cycle.

• Journal of Photoscience
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• v.2 no.2
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• pp.69-72
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• 1995

Dry orderly oriented purple membrane from Halobacterium halobium was obtained by a new technique of preparation. This oriented purple membrane film was very stable, nearly permanently, and showed long term reproducibility with respect to its photochemical behavior. In addition, we have investigated the photooptical properties in terms of the M$_{412}$ intermediate of the bacteriorhodopsin photocycle with respect to the humidity of the film. The relative optical density, i.e. its apparent concentration of the M$_{412}$ intermediate was decreased with the humidity increase as a function of the intensity of the exciting flash within our experimental range. It is suggested that the bound water molecules play an important role in the structure of the bacteriorhodopsin.