• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.2
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• pp.85-99
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• 2004

Purpose: This study observed the changes in the telomerase activity, it's developmental regulation, PCNA expression, and their correlation in rat's upper digestive organs during growth and aging. Materials and Methods: Upper digestive organs(buccal mucosa, gingiva, palate, submandibular and parotid glands, and tongue) were aseptically removed from Sprague-Dawley rats of fetal(gestational 20 days), growing(1, 2, 3, 5, and 7 weeks after birth) and adult(12 week old). Samples for telomerase activity were frozen on liquid nitrogen immediately after sacrifice, and stored until the use at $-75^{\circ}C$ in order to measure it. Telomerase activity was measured by a PCR-based telomeric repeat amplication protoco(TRAP) assay and quantitated with Photometric Telo TAGGG Telomerase PCR ELISA plus(Roche Diagnostics GmbH. Mannheim. Germany). PCNA expression were measured immunohistochemistry with anti PCNA Ab-1, Clone PC10(NeoMark. California. USA). Results: 1. Telomerase activities in buccal mucosa, palate and gingiva were the highest in fetus and decreased gradually or rapidly after birth and then diminished, but In salivary gland and tongue were the highest in fetus and also high at 1 week and then decreased rapidly. 2. PCNA expression in buccal mucosa, gingiva, Tongue and salivary gland was the highest in fetus and decreased gradually and then diminished. but only in palate decreased rapidly after birth and then diminished. Conclusion: The highest telomerase activity of embryonic stage decreased rapidly after birth in rat's upper digestive organs. There may be a developmental regulation of telomerase activity, but not a tissue-specific. This telomerase activity seems correlated closely with PCNA expression in rat's upper digestive system.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.1
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• pp.63.2-63.2
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• 2014

As a part of the "Dust, Ice, and Gas In Time" (DIGIT) key program on Herschel, we observed GSS30-IRS1, a Class I protostar located in Ophiuchus (d =125 pc), with Herschel/Photodetector Array Camera and Spectrometer (PACS). More than 70 lines were detected within a wavelength range from 50 ${\mu}m$ to 200 ${\mu}m$: CO lines from J = 14-13 to 41-40, several $H_2O$ lines of Eup = 100 K to 1500 K, 16 transitions of OH rotational lines, and two atomic [O I] lines at 63 and 145 ${\mu}m$. The [C II] line, known as a tracer of externally heated gas by the interstellar radiation field, is also detected at 158 ${\mu}m$. All lines, except [O I] and [C II], are detected only at the central spaxel of $9^{\prime\prime}.4{\times}9^{\prime\prime}.4$. The [O I] emission is extended along a NE-SW orientation, which is consistent with the known outflow direction, while the [C II] line is detected over all spaxels. One possible explanation of the detection of the [C II] line and no correlation of its spatial distribution with any other molecular emission is the existence of the enhanced ISRF nearby GSS30-IRS1. One interesting feature of GSS30-IRS1 is that the continuum emission is extended beyond the point-spread function (PSF), unlike the molecular line emission, indicative of significant external heating. The best-fit continuum model of GSS30-IRS1 with the physical structure including flared disk, envelope, and outflow shows that the internal luminosity is 11 $L_{\odot}$, and the region is also externally heated by a radiation field enhanced by a factor of 25 compared to the local standard interstellar field.

• Journal of East-West Nursing Research
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• v.6 no.1
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• pp.37-45
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• 2001

The purpose of this study was to identify the effect of hand acupuncture for reduction of dysmenorrhea. 71 female middle school and high school students were selected as subjects from April to May 2001, among which 38 students consisted of the experimental group while 22 students were the control group by convenient assignment. The experimental group participated in hand acupuncture treatment. The experimental treatment was to puncture corresponding points(A4, A5, J1, J3, J23, H2, F7) on both the palm and back of a hand with disposable acupuncture needles and to remove needles after 20-30 minutes' recess during which the subjects reposed themselves on bed. The collected data were processed using the SPSS PC Program and analyzed using $X^2test$ and The results of this study were as follows 1. The symptoms of premenstrual syndrome have decreased, but no significant differences were statistically observed between hand-acupunctured group and non-hand acupunctured group. 2. Subjective pain degree has decreased after hand acupuncture. 3. As for objective pain degree, a significant reduction in voice change was observed between two groups. Facial expressional changes and perspiration degree reduced but showed no significant statistically changes between two groups. 4. Comfort has increased after hand acupuncture. These results suggest that the hand acupuncture therapy works effectively on easing menstrual pain and symptoms as well as partially on reducing discomfort. It is particularly efficacious for a reduction in subjective pain and facial expressional changes in positive respect. Therefore the hand acupuncture therapy can be considered as an independent nursing intervention for dysmenorrhea reduction.

• The Journal of Korean Academy of Prosthodontics
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• v.27 no.1
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• pp.83-102
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• 1989

This study was done to analyze the sound distortion, before and after insertion of the palatal palates. For this study, 4 healthy subjects (3 males and 1 female, each 24-year-old), who were born in Seoul were recruited from K university, and 3 type palatal plates were fabricated, each palatal thickness being 1.0mm, 2.5mm, dentoalveolar portion 2.5mm and elsewhere 1.0mm, named B,C,D-type repectively, and informants's sounds of /사(sa), 서(se), 소(so), 수(su), 스($s\.{+}$), 시(si)/ were recorded, without plate, and with palatal plates of different types, in succession. A series of analysis were adminstered through a 16 Bit IBM PC/AT using linear combination methods. These experiments were analyzed by the Cepstrum (Weighted and Euclidian), Log Area Ratio, Linear prediction correlation methods The findings led to the following conclusions : 1. It was confirmed that the same consonant, /ㅅ(s)/, variously distorted by the following vowel. 2. By and large, 시($s\.{+}$) was the most distorted in all conditions, and (sa), 소(so) were the least distorted in each condition. 3. There were no persistant correlation of the palatal plate types, and sound distortions of each informant were diverse with no regularities. 4. There were persistent correaltion to the Cepstrum (Weighted, Euclidian), Log Area Ratio. However, Linear prediction correlation has a different alteration pattern.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.17 no.32
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• pp.255-266
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• 1994

This study does present things to suggest to foreign multinational corporation or domestic corporation through the empirical study on the standardization level. If we summary this study, it is as follows. In chapter I, we did institute the problems and explain the objects and methods and system of the study. In chapter II, we did explain the universality of the culture and international advertisement. Next, we did explain the background of doing specialization strategy and standardization strategy. Also we did study the previous study about the arguements for and against these. Lsat we did institute the hypothesises as follows. - Hypothesis 1 $H_0$ : The use of westerner appeal means has been increased than 11 years before and is able to increase continuously. - Hypothesis 2 $H_0$ : The use of westerner model and knowned has been increased than 11 years before and is able to increase continuously. - Hypothesis 3 $H_0$ : The use of the English language has been increased than 11 years before and is able to increase continuously. - Hypothesis 4 $H_0$ : The use of the west short piece and background has been increased than 11 years before and is able to increase continuously. In chapter III, we did refer to the method of this study. First, we did adjust the measure of standardization index, and we did work coding it, such after the two coder did check the freguency of the appeal meanstype about the advertisement of four daily report. Then we did select eight production as high involvement production and seven production as low involvement. We did test the coder's trust degree on the such sample to be selected through the 't-test' of the SAS statistic program and regression analysis through the spss/$pc^+$ statistic program to approve the this study's hypothesises. In chapter IV, we did analysis the results of the empirical study on the high or low involvement productions. First, the results of t-test to approve the coder's trust degree is that it is same statistically attentionly the distribution of the sample between coder at 10% attention level. Next, the results of the regression analysis on eight hypothesises are as fellows. In case of high involvement production we are able to speak that the use of the English language appeal among the eight appeals is only standardized. And incase of low involvement production, we are able to speak that the use of the hard-sell appeal among the eight appeds is only standardized. Therefore we did conclude that the standardization level of Korea newspaper advertisement is low. Also this suggests that it will do plan and do the specialzation strategy or localization strategy foreign multinational corporation or domestic corporation in Korea newspaper advertisement. In chapter V, we do present the boundary of this study and new study methods.

• Journal of Periodontal and Implant Science
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• v.25 no.3
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• pp.529-538
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• 1995

It has been believed that the increased release of free oxygen radicals ($O_2^-,H_2O_2$, and $OH^-$) might be a factor in the pathogenesis of periodontal diseases. Antioxidant enzymes such as glutathione peroxidase(GSH-PX) and catalase can protect the tissue damage from the $H_2O_2$. In order to investigate the GSH-PX and catalase activity in the blood plasma and red blood cells(RBCs) of the patients with periodontitis, 19 patients who had good general health, attachment loss more than 6 mm and bone loss were selected as periodontitis group, 7 patients who had severely inflamed gingiva were selected as gingivitis group, and 15 volunteers with good general and periodontal health were selected as normal group. 17 of 26 patients were performed scaling and root planing to reduce the gingival inflammation for gingivitis and periodontitis groups, and were selected as posttreatment group. After blood plasma and RBCs were collected and separated 1 ml of peripheral blood from each subject, GSH-PX activity in blood plasma and RBCs was measured by the same method that Stefan et al. did, and catalase activity in RBCs was measured by the same method that Beers et al. did. The difference of GSH-PX and catalase activity between normal, gingivitis, and periodontitis groups was statistically analyzed by ANOVA with SPSS/PC+ program, and the difference between pretreatment and posttreatment groups was analyzed by Student t-test. The results were as follows : 1. GSH-PX activity in blood plasma was significantly lower in the gingivitis group($0.8683{\pm}0.0658$), periodontitis group($0.7130{\pm}0.1333$) than in the normal group($1.0241{\pm}0.0801$)(p<0.05), and GSH-PX activity in RBCs was significantly lower in the gingivitis groupt. $0.8156{\pm}0.1167$), periodontitis group($0.7533{\pm}0.1185$) than in the normal group($l.1963{\pm}0.2044$)(P<0.05), but there was no statistical significance in the difference of GSH-PX activity in RBCs between the gingivitis group and periodontitis group(p>0.05). 2. Catalase activity in RBCs was siginficantly lower in the periodontitis group($117.34{\pm}35.01$) than in the normal group($l52.38{\pm}32.09$)(p<0.05). 3. GSH-PX activity in blood plasma was significantly increased in the posttreatment groupe $1.0376{\pm}0.2820$) compared to the pretreatment group(0.7608 0.1600) (p<0.05), and GSH-PX activity in RBC was significantly increased in the posttreatment group($1.0421{\pm}0.2330$) compared to the pretreatment group($0.7728{\pm}0.1210$)(p<0.05). 4. There was no statistical significance in the difference of catalase activity in RBCs between the pretreatment group($112.04{\pm}43.65$) and posttreatment group($l33.41{\pm}39.16$)(p>0.05).The results, within the limits of the present experiment, suggest that the lowered activity of GSH-PX and catalase in blood plasma and RBCs may be related with periodontopathogenesis.

• BMB Reports
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• v.39 no.4
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• pp.432-440
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• 2006

A tuber lectin from Arisaema jacquemontii Blume belonging to family Araceae was purified by employing a single step affinity chromatography using column of asialofetuin-linked amino activated silica beads and the bound lectin was eluted with 100 mM glycine-HCl buffer pH 2.5. The purified A. jacquemontii lectin (AJL) showed a single protein band with an apparent molecular mass of 13.4 kDa when submitted to SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The native molecular mass of AJL determined by gel filtration on a Biogel P-200 column was 52 kDa and its carbohydrate content was estimated to be 3.40%. Thus AJL is a tetrameric glycoprotein. The purified lectin agglutinated erythrocytes from rabbit but not from human. Its activity was not inhibited by any of the mono- and disaccharides tested except N-acetyl-D-lactosamine having minimal inhibitory sugar concentration (MIC) 25 mM. Among the glycoproteins tested only asialofetuin was found to be inhibitory (MIC $125\;{\mu}g/mL$). A single band was obtained in native PAGE at pH 4.5 while PAGE at pH 8.3 showed two bands. Isoelectric focusing of AJL gave multiple bands in the pI range of 4.6-5.5. When incorporated in artificial diet AJL significantly affected the development of Bactrocera cucurbitae (Coquillett) larvae indicating the possibility of using this lectin in a biotechnological strategy for insect management of cucurbits. Larvae fed on artificial diet containing sub-lethal dose of AJL showed a significant decrease in acid phosphatase and alkaline phosphatase activity while esterase activity markedly increased as compared to larvae fed on diet without lectin. Out of various human cancer cell lines employed in sulphorhodamine B (SRB) assay, this lectin was found to have appreciable inhibitory effect on the in vitro proliferation of HCT-15, HOP-62, SW-620, HT-29, IMR-32, SKOV-3, Colo-205, PC-3, HEP-2 and A-549 cancer cell lines by 82, 77, 73, 70, 41, 41, 37, 29, 21 and 21% respectively.

• Food Science and Preservation
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• v.24 no.7
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• pp.1043-1051
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• 2017

Wild yeasts were isolated from domestic non-sterilized Makgeolli and their fermentation characteristics were analyzed to select the best fermentation seed culture. A total of 65 yeast strains isolated yeasts from non-sterilized Makgeolli and Nuruk. In order to select fermentable strains, hydrogen sulfide, $CO_2$ production ability, alcohol tolerance and aroma component production ability were analyzed. To screen the aromatic strains of isolates, media containing cerulenin, 5,5,5-trifluor-DL-leucine (TFL) and API ZYM kit were used. There were 36 strains resistance to cerulenin and all strains produced esterase and demonstrated tolerance against TFL. Hydrogen sulfide, which could degrade the quality of the fermented beverage, was not produced in 34 yeast. The correlation between alcohol tolerance of yeast and carbon dioxide production was analyzed by principal component analysis. YM22, YM31, YM32 and YM37 produced a total of 0.14-0.18 g/72 h of $CO_2$ indicating high fermentability. Alcohol tolerance was measured by alcohol concentration. YM32, YM37 yeast had 20% alcohol tolerance. As a result, alcohol and flavor characteristics of wild yeast isolated from non-sterilized Makgeolli were analyzed and it was confirmed that yeast was suitable for the production of alcohol.

• Food Science and Preservation
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• v.24 no.1
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• pp.84-92
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• 2017

Chicken breast jerky (CJ) was prepared by drying chicken breast at $50^{\circ}C$ for 9 hrs after marinating it in a various sweetening sauce including white sugar (WS), brown sugar (BS), rice syrup (RS), fructooligosaccharide (FO), pineapple concentrate (PC), Rubus coreanus extract (RCE), or honey (H), and its physicochemical and sensory properties were investigated. The CJ was found to contain 22.5-25.0% moisture, 41.0-46.6% protein, and 0.4-1.0% fat, which indicates that it could serve as a high-protein and low-fat snack. The type of sweeteners significantly affected the yield, pH, total viable cell count, and water activity of the CJ, showing ranges of 40.9-50.1%, 5.2-5.9, $2.5-6.2{\times}10^4CFU/g$, and 0.74-0.81, respectively. Both the water activity and pH were the lowest in CJ-RCE where of the highest in CJ-WS. The cohesiveness, springiness, and chewiness of the CJ significantly differed depending on the type of sweeteners (p<0.05). CJ-RCE showed the best taste and overall acceptability in a sensory test. After storage at $50^{\circ}C$ for 2 weeks, thiobarbituric acid reactive substance (TBARS) content (58.3 malondialdehyde (MDA) mg/kg) of CJ-RCE was much lower than those of control beef (75.6 MDA mg/kg) and pork jerky (98.0 MDA mg/kg), showing the good oxidative stability of CJ-RCE. Overall, marination in RCE sauce was suitable for the preparation of CJ with good quality in terms of its water activity, fat and protein contents, sensory property and oxidative stability.

• Journal of Digital Contents Society
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• v.9 no.2
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• pp.203-211
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• 2008

Due to the advance of H/W and S/W techniques to play 3D sound, the virtual space contented by 3D graphics and sounds can provide users more improved realities and vividness. However for the small 3D contents developers and companies, it is hard to implement 3D sound techniques because the implementation requires expensive sound engines, 3D sound technical understanding and 3D sound programming skills. Therefore 3D-sound-playing-interface is necessary to easy and cost-effective 3D sound implementation. Using this interface, graphics experts can easily add 3D sound techniques to their applications. In this paper, the followings are designed and implemented as a preliminary stage in the way of developing the 3D sound playing interface. First, we develop 3D sound S/W modules converting mono to 3D sound in PC based systems. Second, we develop the interconnection modules to map 3D graphic objects and sound sources. The developed modules in this paper can allow the user to percept sound source position and surround effect at the moving positions in the virtual world. In the coming works, we are going to develop the more completed 3D sound playing interface consisted of the synchronization technique for sound and moving objects, and HRTF.