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Effects of Simulated Acid Rain on Growth and Physiological Characteristics of Ginkgo biloba L. Seedlings and on Chemical Properties of the Tested Soil -III. Effects on Chemical Properties of the Tested Soil- (인공산성우(人工酸性雨)가 은행(銀杏)나무 Ginkgo biloba L. 유묘(幼苗)의 생장(生長), 생리적(生理的) 특성(特性) 및 토양(土壤)의 화학적(化學的) 성질(性質)에 미치는 영향(影響) -III. 토양(土壤)의 화학적(化學的) 성질(性質)에 미치는 영향(影響)-)

  • Kim, Gab Tae;Lee, Don Koo
    • Journal of Korean Society of Forest Science
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    • v.77 no.1
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    • pp.43-52
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    • 1988
  • One-year-old seedlings of Ginkgo biloba were treated with various simulated acid rains(pH 2.0, pH 3.0, pH 9.0 and pH 5.0) to examine the effects of simulated acid rain on the chemical properties of the tested soil. The seedlings were grown in a pot($4500cm^3$)containing one of three different soils(nursery soil, mixed soil and sandy soil). Simulated acid rain was made by diluting sulfuric and nitric acid solution($H^1SO^4$ : $HNO^3$ =3 : 1, V/V) with tap water and tap water(pH 6.4), and treated by 5mm each time for three minutes during the growing seasons(April to October 1985). Acid rain treatments were done three times per week to potted seedlings by spraying the solutions. The chemical properties of potting media were compared among three soil types as well as among the various pH levels. The results obtained in this study were as follows : 1. Exchangeable calcium and magnesium contents and base saturation of the soil decreased with decreasing pH levels of acid rain, and their decreasing rates were as follows : sandy soil was the highest, followed by mixed and nursery soils, However, exchangeable aluminum content rather increased as the pH levels decreased. 2. Available phosphate in the soil decreased as the pH levels of acid rain decreased. Its content increased in nursery soil, compared with those before acid gain treatment, Gut decreased in mined and sandy soils. 3. Soil sulfate and nitrate contents increased remarkably as the pH levels decreased, and the only significant difference in the sulfate was found among the pH levels. Soil sulfate content was the highest in nursery soil, followed by mixed and sandy soils.

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Comparative Evaluation of Colon Cancer Stemness and Chemoresistance in Optimally Constituted HCT-8 cell-based Spheroids (적정 구성 배양 HCT-8 기반 대장암 스페로이드의 암 줄기세포능 및 항암제 내성 평가의 비교 평가 연구)

  • Lee, Seung Joon;Kim, Hyoung-Kab;Lee, Hyang Burm;Moon, Yuseok
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1313-1319
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    • 2016
  • Cancer is a complex disease heterogeneously composed of various types of cells including cancer stem-like cells responsible for relapse and chemoresistance in the tumor microenvironment. The conventional two-dimensional cell culture-based platform has critical limitations for representing the heterogeneity of cancer cells in the three-dimensional tumor niche in vivo. To overcome this insufficiency, three-dimensional cell culture methods in a scaffold-dependent or -free physical environment have been developed. In this study, we improved and simplified the HCT-8 colon cancer cell-based spheroid culture protocol and evaluated the relationship between cancer stemness and responses of chemosensitivity to 5- Fluorouracil (5-FU), a representative anticancer agent against colon cancer. Supplementation with defined growth factors in the medium and the culture dish of the regular surface with low attachment were required for the formation of constant-sized spheroids containing $CD44^+$ and $CD133^+$ colon cancer stem cells. The chemo-sensitivities of $CD44^+$ cancer stem cells in the spheroids were much lower than those of $CD44^-$ non-stem-like cancer cells, indicating that the chemoresistance to 5-FU is due to the stemness of colon cancer cells. Taken together, the inflammation and oncogenic gut environment-sensitive HCT-8 cell-based colon cancer spheroid culture and comparative evaluation using the simplified model would be an efficient and applicable way to estimate colon cancer stemness and pharmaceutical response to anticancer drugs in the realistic tumor niche.

Characteristics of Enterobacteria from Harmonia axyridis and Effects of Staphylococcus spp. on Development of H. axyridis (무당벌레(Harmonia axyridis ) 장내세균의 특성 및 Staphylococcus spp. 장내세균이 무당벌레의 발육에 미치는 영향)

  • Moon, Chung-Woun;Kim, Ki-Kwang;Whang, Kyung-Sook;Seo, Mi-Ja;Youn, Young-Nam;Yu, Yong-Man
    • Korean journal of applied entomology
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    • v.50 no.2
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    • pp.157-165
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    • 2011
  • Enterobacteria were isolated in the gut of the predacious multicolored Asian ladybird beetle, Harmonia axyridis, and their effects to the development of H. axyridis were examined. Populations of H. axyridis in this experiment were collected from Kimjae at Cheonbuk province (JK population), Geumsan at Chungnam province (CK population) and laboratory population at Laboratory of Insect Physiology in Chungnam National University, Daejeon. Thirty-four enterobacteria isolates were purified and isolated from the digestive tract of H. axyridis, and a total of 4 strains were classified into group by analysis of 16S rRNA gene sequences. About 70% of total isolates were phylogenetic groups of Bacillus genus and Staphylococcus genus, and they were commonly separated from the digestive tract of H. axyridis. After investigating their susceptibility against antibiotics with 18 representative enterobacteria isolates, ofloxacin and penicillin were selected for examination in this study of their ability to inhibit the growth of all of isolates. In order to remove the enterobacteria from the aphids, ofloxacin and penicillin were given to the green peach aphid, Myzus persicae, and the turnip aphid, Lipaphis erysimi. These aphids were provided to H. axyridis as prey. The weight of pupa, developmental periods of each larval instar, the number of eggs and their hatching ratio of H. axyridis with treatment aphids were lower compared with non-treatment aphids. Staphylococcus saprophyticus is a representative enterobacteria and commonly isolated from the digestive tract of H. axyridis. In the absence of S. saprophyticus, the developmental periods of each larval instar increased; however, the weights of pupa, the number of eggs, and their hatching ratio decreased.

Clinical Manifestations of Norovirus Infection in Korean Pediatric Cancer Patients (한국 소아 암환자에서 노로바이러스 감염증의 임상 양상)

  • Choi, Hyunshin;Choi, Young Bae;Hwang, Ji-Young;Cheon, Doo-Sung;Jeong, Hye Sook;Choe, Yon Ho;Yoo, Keon Hee;Sung, Ki Woong;Koo, Hong Hoe;Kim, Yae-Jean
    • Pediatric Infection and Vaccine
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    • v.18 no.1
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    • pp.40-47
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    • 2011
  • Purpose : Norovirus infection, a common cause of community-acquired gastroenteritis, can also lead to severe illness in immunocompromised patients. We investigated clinical manifestations of norovirus infection in pediatric cancer patients. Methods : Stool specimens were collected from pediatric patients with gastrointestinal symptoms between November 2008 and September 2009 at Samsung Medical Center, Seoul, Korea. Norovirus infection was identified by reverse-transcription polymerase chain reaction (RT-PCR). A retrospective chart review was performed in pediatric cancer patients who were diagnosed with norovirus infection. Results : Ten patients were diagnosed with norovirus infection by RT-PCR in stool samples. The median age was 0.83 years (range 0.25-5.5 years) and the male to female ratio was 1.5:1 (6 males and 4 females). Underlying diseases were hematologic malignancies (4/10, 40%), neuroblastoma (4/10, 40%), and brain tumors (2/10, 20%). Three patients were infected before hematopoietic cell transplantation (HCT) and four patients after HCT. All patients had diarrhea (10/10, 100%), with a median frequency of diarrhea of 8.5 times/day (range 4-22 times/day). Median virus shedding duration was 72.5 days (range 19-299 days). Four patients with pneumatosis intestinalis were conservatively treated with bowel rest and total parenteral nutrition. One patient with severe diarrhea and bloody stool had concomitant chronic gut graft-versus-host disease (GVHD). Norovirus infection-related mortality was not observed. Conclusion : Norovirus infection can cause significant clinical manifestations with prolonged viral shedding in immunocompromised patients. Norovirus should be considered in pediatric cancer patients with severe gastrointestinal symptoms.

Effects of Dietary Mulberry Leaf on Loperamide-Induced Constipation in Rats (식이뽕잎이 흰쥐의 Loperamide로 유도된 변비에 미치는 영향)

  • Lee, Jae-Joon;Lee, Yu-Mi;Jung, Seoung-Ki;Kim, Keun-Young;Lee, Myung-Yul
    • Food Science and Preservation
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    • v.15 no.2
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    • pp.280-287
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    • 2008
  • We investigated the effect of dietary mulberry leaf powder (MP) on loperamide-induced constipation in rats. Male Sprague-Dawley rats were given MP in their diets at a concentration of 0% 5% and 10% for 33 days. Rats were divided into 4 groups: normal diet group (NOR), normal diet and loperamide treated group (MPL0), 5% MP and loperamide treated group (MPL5), and 10% MP and loperamide treated group (MPL10). Constipation was induced by subcutaneous injection of loperamide (1.5 mg/kg body weight/day) for the final 5 days of the experiment Supplemental MP had no effect on the food efficiency ratio, but it reduced body weight gain and food intake in a concentration dependent manner. Administration of loperamide decreased food intake. MP had a concentration-dependent effect on decreasing total cholesterol, LDL-cholesterol, and triglycerides and on increasing HDL-cholesterol. Loperamide had no significant effect on serum lipid profiles. Loperamide decreased the number and wet weight of fecal pellets and fecal water content MP increased the number and wet weight of fecal pellets and fecal water content in a dose-dependent manner. In addition, MP increased gut transit time and transit speed, and the guts of mts treated with MP plus loperamide were longer than those of mts treated with loperamide alone. These results indicate that MP is an effective treatment for constipation.

A study model standardization by he body types of Jugori of Hanbok for middle-aged women (중년 여성을 위한 한복 저고리의 체형별 원형 연구)

  • 진현선;권미정
    • Journal of the Korea Fashion and Costume Design Association
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    • v.5 no.1
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    • pp.13-24
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    • 2003
  • The purpose of this study is to design Jugori model compatible with the body types of the middle-aged women especially from 40 to 59 years old. The result is as follows: We decided five items as the necessary items for designing jugori model : the bust girth (the breast & shoulder width), the B.P length, the neck width, the armhole circumference, and Hwa-jang. The breast & shoulder width are the size that comes out if the bust is divided by the breast & shoulder width on the basis of the side line, and Hwa-jang is a length measured with arms stretched out to 0° direction. With each person's physical characteristics considered, the application of the size of each body types and body parts is as follows: 1. The breast & shoulder width (1/4 portion) : We decided B/4+2cm as a standard size and, we adjusted the extra room on the basis of the discrepancy between the breast width and the shoulder width to make it fit well to the each body type. For the breast width (1/2 portion), we bisected the difference between the breast width and the shoulder width of the bust, and moved Gut-sup to the center of the Sup and Sup-sun for An-sup. According to the body type, the movement of the Sup for the people with big breasts gets bigger because there should be a big difference between the breast width and the shoulder width for them, and for the people with small breasts the movement will be relatively smaller. For the shoulder width (1/2 portion), we curved the back center line after we shortened as much as the difference between the amount of the shoulder width/2+1cm and of B/4+2cm. The movement of back center line will be bigger for a person with leaned-backward body type. 2. The front & back length: We made the front length to B.P length+2.5cm to have Jugori cover the breast point fully around the bust line, which is a vogue nowadays. For an upright body type, we decided the back length as (AH/2.2)+5cm. And for a bent-forward and a leaned-backward body type, we adjusted the calculation formulae differently taking the physical characteristics into account. We decided the back length (A) as (A.H/2.2)+5cm, and the front length (B) as the back length+5cm. So, (A+B) is the sum of the front length and the back length. Going back to the original formula, the front length is B.P+2.5cm. So, we can decide the back length if we subtract B.P+2.5cm from the sum of the front length and the back length. To make well-fit Jugoris, the front & back length are areas that we should pay attention to if we take each person's physical characteristics into consideration. 3. Go-dae (1/2 portion) : We decided Go-dae as the neck width/2+0.5cm. For an upright body type, because the base line which went down vertically from the tragion was straight, we generally decided Go-dae Dalim line as 1.0cm. But we decided Go-dae Dalim line down to 1.5cm for bent-forward type and up to 0.2cm for leaned-backward type because the upper half of the body of them was bent forward or leaned backward from the base line. 4. The armhole : We decided the armhole circumference as A.H/2+2cm with the whole extra room of 4cm. 5. The side line length : We can calculate the side line length to (the back length-the armhole)/2, and, in terms of the trend, 2.5cm will be appropriate.

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Seedling Production of Rabbitfish, Siganus canaliculatus (흰점독가시치, Siganus canaliculatus의 종묘생산)

  • Hwang, Hyung-Kyu;Lee, Jung-Uie;Rho, Sum;Yang, Sang-Geun;Kim, Seong-Cheol;Kim, Kyong-Min
    • Journal of Aquaculture
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    • v.13 no.4
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    • pp.277-284
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    • 2000
  • Rabbitfish hatchlings were given a mixed food of rotifers (Brachionus rotundiformis) and trochophore larvae of oyster. Only the oyster-trochophore larvae were found in the gut of 62-h old fish larvae. The fish larvae, fed on rotifer and ciliate alone did not survive. However, their survival increased to 3.3 % on the 10th day after hatching, when trochophore was supplemented. Corresponding with the accelerated growth, the number of rotifers consumed increased from 11 in a 5-day old fish to 165 in a IS-day old fish. In a field ecosystem containing live diatom, Nannochloropsis oculata, rotifers and copepods, fish larvae were shocked and the oyster's trochophore larvae were fed from 2 to 7 days after hatching. A total of 76,000 seedling were produced after 50 days of hatching with 12.7 % survival. Mean total length and body weight were 65.6 mm and 3.4 g, respectively. Growth of body length (BL), body height (BH), body weight (BW) and head length (HL) as a function of the total length (TL) showed regressional relationships as follows; BL=0.8565 TL+0.0852 ($t^2$=0.9996); BH=0.3207 TL - 0.5052 (($t^2$=0.9641) BW=0.0652 TL2.3508 (($t^2$=0.9925); HL=0.2595 TL - 0.1898 (($t^2$=0.9901)

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Die $Luftfrachtersatzbef{\ddot{o}}rderung$ (항공화물대체운송(航空貨物代替運送)에 관한 법적(法的) 문제(問題))

  • Jeon, Sam-Hyeon
    • The Korean Journal of Air & Space Law and Policy
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    • v.9
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    • pp.327-349
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    • 1997
  • Luftfrachtersatzverkehr ist der Transport von Luftfrachtgut von Flughafen zu Flughafen unter einem Luftfrachtbrief im $Oberfl{\ddot{a}}chentransport$ urn die erste und/oder letzte Teilstrecke einer als Gesamtstrecke vereinbarten $Luftfrachtbef{\ddot{o}}rderung$. These Strecken $f{\ddot{u}}hren$ von nationalen oder intemationalen $Flugh{\tilde{a}}fen$ zurn Zentralflughafen ($_"Hub"$) der Luftverkehrsgesellschaft bzw. in urngekehrter Richtung vom Zentralflughafen hin zu den einzelnen nationalen oder intemationalen $Flugh{\ddot{a}}fen$. Die Vorteile des Luftfrachtersatzverkehrs sind offenkundig, daher auch sein $st{\ddot{a}}ndiges$ Anwachsen. Allerdings bestand bei den Luftverkehrsgesellschaften jahrelang eine $gro{\ss}e$ Unsicherheit, wie dieser Luftverkehr, der eben nicht in der Luft $durchgef{\ddot{u}}hrt$ wird, rechtlich zu qualifizieren sei. Stellte die $Bef{\ddot{o}}rderung$ von $Luftfrachtg{\ddot{u}}tem$ auf der $Stra{\ss}e$ einen Bruch des $Luftbef{\ddot{o}}rderungsvertrages$ dar, so wie im Schadensfall die $Gesch{\ddot{a}digten$, Absender oder $Empf{\ddot{a}}nger$ des betreffenden Gutes, der Luftverkehrgesllschft auf Art. 18 Abs. 3 oder Art. 31 Warschauer Abkommen(WA) berufen? Folge dieser Unsicherheit war, $da{\ss}$ ${\ddot{u}}ber$ lange Jahre hinweg die Luftverkehrsgesellschaften sich scheuten, diese Frage einer gerichtlichen $Kl{\ddot{a}}rung$ $zuzuf{\ddot{u}}hren$, Eher war man geneigt, sich mit dem Anspruchsgegner $au{\ss}ergerichtlich$ zu vergleichen, selbst wenn dies bedeutete, $da{\ss}$ man sich nicht auf die $Haftungsbeschr{\ddot{a}}nkungen$ des Art. 22 WA berufen konnte, als ein Urteil zu erstreiten, welcher $m{\ddot{o}}glicherweise$ der Praxis der $Luftfrachtersatzbef{\ddot{o}}rderung$ einen - rechtlichen - Riegel vorgeschoben $h{\ddot{a}}tte$. Diese Unsicherheit ist jedoch durch die Entscheidung die erste und wohl auch bislang einzige $h{\ddot{o}}chstrichterliche$ Entscheidung zur $Luftfrachtersatzbef{\ddot{o}}rderung$ nicht nur in Deutschland, sondem soger in Europa. Die Luftverkehrsgesellschaften $k{\ddot{o}}nnen$ mit dieser Entscheidung gut leben. Bei emer $vertragsgem{\ddot{a}}{\ss}en$ $Luftfrachtersatzbef{\ddot{o}}rderung$ haftet der $Luftfrachtf{\ddot{u}}hrer$ $f{\ddot{u}}r$ $Sch{\ddot{a}}den$ $anl{\ddot{a}}{\ss}lich$ der mit dem Luftfahrzeug $ausgef{\ddot{u}}hrten$ Teilstrecke nach den Vorsschriften des Warschauer Abkommens und $f{\ddot{u}}r$ $Sch{\ddot{a}}den$ $anl{\ddot{a}}{\ss}lich$ der mit einem $Oberfl{\ddot{a}}chenbef{\ddot{o}}rderungsmittel$ $ausgef{\ddot{u}}hrten$ Teilstrecke nach den Vorschriften, welche $f{\ddot{u}}r$ das $tats{\ddot{a}}chlich$ eingesetzte ransportmittel $einschl{\ddot{a}}gig$ sind. Bei unbekanntem Schadensort haftet der $Luftfrachtf{\ddot{u}}hrer$ nach dem jeweils $sch{\ddot{a}}rfsten$ der in Betracht kommenden Rechte. Bei emer vertragwidrigen $Luftfrachtersatzbef{\ddot{o}}rderung$ haftet der $Luftfrachtf{\ddot{u}}hrer$ nach dem Recht des vereinbarten $Bef{\ddot{o}}rderungsmittels$, da sich auf dieses Recht der Vertragspartner des $Luftfrrachtf{\ddot{u}}hrers$ eingerichtet hatte. Der $Luftfrachtf{\ddot{u}}frer$ haftet aber auch nach dem Recht des $tats{\ddot{a}}chlich$ eingesetzten $Bef{\ddot{o}}rderungsmittels$, da er sich in dessen Haftungsordnung durch die einseitige Wahl des $Bef{\ddot{o}}rderungsmittels$ selbst hineingestellt hat. Bei unbekanntem Schadensort haftet der $Luftfrachtf{\ddot{u}}hrer$ nach dem jeweils $sch{\ddot{a}}rfsten$ der in Betracht kommenden Rechte.

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The Philosophy of Good and Evil Engraved on Roof - End Tiles - A Contemplation of "The Smile of Silla" Roof-End Tiles (수막새에 새겨진 선악의 철학 -신라의 미소, 수막새를 통한 고찰-)

  • Yun, Byeongyeol
    • Korean Journal of Heritage: History & Science
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    • v.53 no.1
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    • pp.4-23
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    • 2020
  • This study examines the universal meaning of the roof-end tile, our cultural property, and especially focuses on an ontological interpretation of the "Smile of Silla" roof-end tile. In addition, the problem of good and evil read here is considered in connection with the universal problem of philosophy. The issue of good and evil is a theme in philosophy, theology, religion, and culture that will endure throughout human history in both the East and the West. Augustine and Schelling inquired deeply into the source of evil and obtained an answer to this question based on their methods, but their answer is not universal or absolute, or an answer that applies to everyone. This is because the issue of good and evil possesses both a direct relationship with every human being and a characteristic that will remain unresolved. That is to say, the metaphysical question regarding the source of evil will always be one that is open. Nietzsche, however, repudiated the morals handed down through Socrates and Christianity, and urged that we reside "beyond good and evil." This brief review argues that good and evil exists in the form of a being in itself, whether it is within our grasp or not, and reveals that good and evil is more "this-worldly" than it is "other-worldly". The roof-end tiles with facial markings passed on to us also presuppose that evil is in full force in this world and exerts its influence. This review taps into several folk methods for coping with the existence of an invincible evil that surpasses human capability and contemplates the extraordinary and creative ideas of the Silla people through their "Smile of Silla" roof-end tiles with facial markings that were used to counter evil.

Effect of starch extraction solutions on extraction and physicochemical property of Chinese yam (Dioscorea batatas) starch (마(Dioscorea batatas) 전분의 추출 및 물리화학적 특성에 대한 전분추출용액의 영향)

  • Lee, Hyunjeong;Seo, Dong-Ho;Kim, Hyun-Seok
    • Korean Journal of Food Science and Technology
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    • v.50 no.2
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    • pp.191-197
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    • 2018
  • This study investigated the influence of starch extraction (ST-EX) solutions on the extraction yield and characteristics of Chinese yam (CY) starches from domestic Dioscorea batatas. Ascorbic acid (VitC), $Na_2S_2O_3$, $NaHCO_3$, and $Na_2CO_3$ were used as ST-EX solutions (0.4%, w/v). The extracted CY starches were examined for ST-EX yield, chemical composition, size distribution, X-ray diffraction, solubility, swelling power, gelatinization, and pasting viscosity. The highest ST-EX yield was obtained from $NaHCO_3$, followed by VitC. Lower protein content, relative crystallinity, and gelatinization enthalpy were found in CY starches from alkaline ST-EX solutions ($NaHCO_3$ and $Na_2CO_3$). Size distribution and gelatinization temperature did not generally differ for CY starches from the different ST-EX solutions. Pasting viscosities increased in the order from $Na_2CO_3$ > $Na_2S_2O_3$ > $NaHCO_3$ > VitC ST-EX solutions. Thus, VitC may be most appropriate to extract CY starch from Dioscorea rhizomes, considering its ST-EX yield, total starch content, and variation in pasting viscosity.