• Journal of Microbiology and Biotechnology
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• 제2권2호
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• pp.141-151
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• 1992

Physiological changes of the murine hybridoma cell line $S3H5/\gamma{2bA2}$ during adaptation to RPMI 1640 medium with 1%(v/v) fetal bovine serum were characterized in terms of cell growth, antibody production, morphology, and metabolic quotients. Cells adapted to 1% serum medium in T-flasks became sensitive to shear induced by mechanical agitation and required at least 5% serum in the medium or spent medium for cell growth in spinner flasks, while cells adapted to 10% serum medium in T-flasks could grow in 1% serum medium in spinner flasks. Consequently, long-term adaptation to low serum media may not give the expected growth enhancement. After adaptation to 1% serum medium, changes in cell morphology were observed. The cells in 10% serum medium were uniform and circular, while cells in 1% medium were irregularly shaped. The DNA contents, which were measured by flow cytometry, were almost constant among the cells in the range of 1% to 10%. Further, no significant changes in energy metabolism and specific monoclonal antibody production rate were observed among these cells.

• 한국균학회지
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• 제11권2호
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• pp.79-84
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• 1983

Armillariella mellea의 2차균사를 potato dextrose medium, yeast extract medium, malt extract medium Hamada medium, 등에 배양해서 생리 생태적 성질을 연구했다. 그 결과를 요약하면 아래와 같다. 1. 사용했던 4종의 배치 중에서 malt extract medium에서 가장 잘 자랐고, 그 다음 yeast extract medium, potato dextrose medium 그리고 Hamada medium 순으로 생육율이 높였다. 2. $27^{\circ}C$에서 가장 잘 자랐고, $36^{\circ}C$에서는 집락의 지름이 $27^{\circ}C$와 비슷하나 균사의 밀도가 매우 낮았다. 3. pH6에서 가장 높은 생육율을 나타내었다. 4. 식물 생장 호르몬을 첨가한 경우에는 Heteroauxin의 5ppm 농도에서 대조군보다 26.2% 증가로 가장 많은 생육 증량을 보이고, 다음으로 gibberellin 100ppm, Tomatoton 10,000배액, 그리고 Adoton 5,000배액의 순서였다. 식물 생장 호르몬을 적당 농도로 사용하면 생장 증량 효과를 얻을 수 있으나, 과량 사용할 경우, 오히려 생장 억제 효과를 나타내었다. 5. Vitamin 첨가경우에 있어 200ppm 농도의 균일한 조건하(下)에서 Vit.A 첨가군에서 대조군보다 18.2%의 가장 많은 생육 증량을 보였고, $Vit{\cdot}D_2\;Vit{\cdot}E,\;Vit{\cdot}B_1\;folic\;accid\;Vit{\cdot}C$ 그리고 $Vit{\cdot}B_2$의 순으로 그 증가가 나타나 지용성 비타민 첨가 배지에서 상당한 생육율을 나타내었다.

• 한국약용작물학회지
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• 제20권3호
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• pp.190-194
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• 2012

Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 mg/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 mg/L BA and 0.5~1.0 mg/L IAA. Sucrose added to MS medium with 2.0 mg/L BA and 0.5 mg/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks' culture of micro-tuber sections on the medium with 2.0 mg/L BA, 0.5 mg/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 $m{\ell}$ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.

• KSBB Journal
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• 제14권2호
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• pp.167-173
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• 1999

A new synthetic medium was developed for the submerged mycelial cultures of Phellinus linteus. The medium for maximum mycelial growth of Phellinus linteus (3 days incubation, 28$^{\circ}C$, pH 5) consisted of (per 1 L): glucose, 90 g peptone, 10 g soluble starch, 10 g yeast extract, 3 g KH2PO4, 1 g MgSO4.7H2O, 1 g and CaCl2, 0.1 g. The concentrations of glucose, peptone, yeast extract, KH2PO4, MgSO4.7H2O, and CaCl2 were examined in the ranges of 10~90 g/L, 0~10 g/L, 0~15 g/L, 0~2 g/L, 0~1 g/L, and 0~0.5 g/L, respectively. The dry weight of mycelium in 3 days increased to 16.79 mg/mL using the new synthetic medium. The optimum temperature for mycelial growth of Phellinus linteus was 28$^{\circ}C$. The concentrations of KH2OP4, CaCl2, and yeast extract, which gave the maximum mycelial growth of Phellinus linteus, existed in the concentration ranges examined in this study. But, in the cases of other compositions (MgSO4.7H2O, peptone, and glucose), the mycelial growth of Phellinus linteus increased with the concentration in the ranges.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.849-854
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• 2006

The effects of Haematococcus pluvialis extracellular products on microbial growth and bacteriocin production were investigated to improve bacteriocin synthesis during the growth cycle of Lactobacilli. Lactobacillus pentosus KJ-108, L. plantarum KJ-10311, and L. sakei KJ-2008 were cultured in MRS and enriched medium (ERM) with or without supplement of the extracellular products obtained from a late exponential phase culture of Haematococcus pluvialis in modified Bold's basal medium (MBBM). In both MRS and ERM, the extracellular products strongly enhanced the growth as well as the bacteriocin production of all the lactic acid bacteria tested. The enhancing effect was observed in ERM with pH adjusted at 5 and 6. In addition, some difference in growth effects with the extracellular products of H. pluvialis was observed between pH 5 and 6 in ERM, but no effect was observed in the minimal medium. The final biomass and the final concentration of bacteriocin activity were associated with the cell growth that was promoted by the extracellular products of H. pluvialis, and the enhanced cell growth of the three lactic acid bacterial strains induced the increase of the specific bacteriocin production. Therefore, bacteriocin production and activity were influenced by the addition of the extracellular products of H. pluvialis in the culture medium.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.87-90
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• 2004

An efficient plant regeneration system in alfalfa (Medicago sativa L.) through somatic embryogenesis was established. Embryogenic callus was obtained by culture of hypocotyl segments on MS medium with 0.02mg $L^{-1}$ IAA and 1.0mg $L^{-1}$ zeatin after 45 days of culture. Embryogenic calli were converted to the somatic embryos when transferred to either MS medium without plant growth regulators (PGRs) or MS medium containing various cytokinin (BA, kinetin and zeatin). Most of the somatic embryos were developed into plantlets on MS medium supplemented with 0.1 mg $L^{-1}$ kinetin. Also, secondary embryos appeared on the surface of primary embryo but they showed abnormal growth. Regenerated plantlets were transplanted to pots containing vermiculite and perlite for further analysis.

• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.149-153
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• 1995

A simple and low-cost medium was developed for the growth of Bifidobacterium longum KFRI 977. Of three bifidobacterial strains, B. longum KFRI 977 (ATCC 15707) showed the best growth in MRSC broth containing 0.3% oxgall, grew well in partially anaerobic condition, exhibited highest $\beta$-galactosidase activity, and was inhibitory against Clostridium perfringens KFRI 434. Of three developed media, the population of B. longum KFRI 977 was highest (1.9$\times$$10^9$/ml) in ISP based medium. The composition of ISP based medium is ISP (5%), glucose (1%), L-cysteine HCI (0.05%), Trypticase peptone (0.5%), yeast extract (0.5%), $MgSO_4$ (0.05%), Tween-80 (0.1%), and phosphate buffer (pH 7.0). Hydrolysis of ISP by Protease A was unnecessary, and the use of phosphate buffer (pH 7.0) prevented the formation of protein precipitate. Associative culture of B. longum KFRI 977 with Lactobacillus acidophilus KFRI 233 was proven to be deleterous to the growth of B. longum KFRI 977.

• 한국식품과학회지
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• 제10권2호
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• pp.189-193
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• 1978

상기(上記) 실험(實驗)에서는 Agaricus campestris의 basal medium에 몇종(種)의 생즙(生汁)을 첨가(添加)하여 균사생장(菌絲生長)에 미치는 영향(影響)을 조사(調査)하였다. 이때 실험실(實驗室) 온도(溫度)는 $23{\pm}1^{\circ}C$, 습도(濕度)는 $70{\pm}5%$, medium pH $5.4{\sim}5.5$로 하였다. 위 환경(環境) 조건(條件)에서 자란 결과(結果)는 다음과 같다. (이상(以上)은 144hrs후(後) 성적(成積)이다) 1. Carrot basal medium에서는 100ml의 basal medium에 4ml 의 carrot extract를 첨가(添加)할때가 control에 비(比)해 가장 좋은 유의적(有意的)인 성적(成積)을 나타냈고 Cucumber basal medium에서는 100ml basal medium에 3ml의 cucumber extract를 첨가(添加)했을 때가 우세(優勢)하였으며 radish basal medium에서는 100ml의 basal medium에 radish extract 3ml 첨가시(添加時) 성적(成積)이 양호(良好)하게 나타남으로써 유의적(有意的)인 차이(差異)를 보였다. 2. cabbage basal medium에서는 100ml의 basal medium에 2ml의 cabbage extract를 첨가(添加)했을 경우(境遇)에 control에 비(比)해 약간(若干) 우위(優位)였으나 대차(大差)없었고 Lettuce basal medium 역시(亦是) 100ml의 basal medium에 2ml의 lettuce extract를 첨가(添加)했을 경우(境遇)만 조금 성적(成積)이 좋았을 뿐이다.

• 한국자원식물학회지
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• 제11권
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• pp.40-47
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• 1998

When the leaves, roots and stem segments of seedling of Polygonatum odoratum were cultured on Murashige and Skoog medium with 2.0mg/l BAP, stem segments were the most efficient explants for adventitious shoot inductino. To observe the efficient combination of growth regulators on the adventitious shoot formation , stem segments were cultured on MS medium with various kinds of cytokinins (BAP, kinetin, zeatin). From this experiment, cytokinin treatement was prerequisite for theadventitious shoot formatino,especially BAP was the most effective. Auxin (NAA or IBA) in combination with cyotokinin highly enhanced the adventitious shoot formation. Twenty five percents of explants produced the adventitious shoots on medium with 2.0mg/l BAP solely, while 83% of explants produced the adventitious shoots on medium with 2.0mg/l BAP and 0.1mg/l IBA. Root formationform adventitious shoot was promoted after transfer to 1/2 MS medium supplemented with 0.1mg/l IBA and 0.5mg/l zeatin, thereafter the plantlets with shoots and roots were cultured on 1/2MS medium lacking growth regulators. When the stem segments were cultured to MS medium with 1.0mg/l 2,4 NAA and IBA , yellow and nodulous cali were formed from the stem segments which were developed into adventitious roots. These roots were actively grew after transferred to MS liquid medium lacking growth regulators.

• Fisheries and Aquatic Sciences
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• 제3권2호
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• pp.151-155
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• 2000

Phaeodactylum tricornutum (Bacillariophyceae) is a marine diatom which has been supplied as a food of bivalves. In this study, growth responses of P. tricornutum to some nitrogen sources and sodium were investigated by measuring cell number and contents of chlorophyll a in culture. In medium with nitrogen and sodium, brisk cell division occurred and maximum growth rate was respectively found in the medium with 150 mg/l of nitrate and 10 mg/l of ammonium and urea. At 10-500 mg/l ammonium and urea and 200-500 mg/l nitrate, specific growth rate decreased slightly. However, no cell division observed in sodium-deficient medium, regardless of presence or absence of nitrogen. This suggests that sodium is required for the nitrogen uptake of P. tricornutum, resulting nitrogen uptake leading to cell division. Also the upper limits of ammonium and nitrate for the growth of P. tricornutum seem to be 10 mg/l and 500 mg/l, respectively.