• Asian Journal of Turfgrass Science
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• v.7 no.1
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• pp.5-12
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• 1993

This experiment was conducted to study on the morphological change and growth retardation of Korean Lawograss (Zoysia japonica Steud.) under various shading degrees. Dada of plant height, No. of tiller, No. of stolon, No. of rhizome and fresh weight were measured on the l47days after transplanting in 1991. The results are summarized as follows. Plant height was increased at 30% shading degree or over, Maximum plant height was observed at 60% shadeing degree which was 24.2 cm. while full sunlight(control) was most shorted as 10.1 cm. Plant height rapidly was increased as to shading degree increase. The decreasing rate was lower in tiller number and stolon number(top parts of plant) compared with the rhizome number(under ground part) was severely decreased at 30% to 60% of shading. To increase the shading degree, rhizome number was most severely decreased under shading degrees. As degree of shading increase to 30%, 60% and 90%, fresh weight decrease to 66%, 44%, 22% to full sunlight, respectively. Relative growth retadation of Korean lawngrass was decreased by the order of number of rhizome, fresh weight, number of tiller, number of stolon. And these characters were highly significant accord-ing to the different shading degrees.

• Asian Journal of Turfgrass Science
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• v.6 no.1
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• pp.29-37
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• 1992

This experiment was carried out in order to study the changes of morphological characters of the growth and thatch accumulation in :3 varieties of Kentucky bluegrass under removing clipping residues as affected by cutting management. The varieties used were Park, Kenhiuc and Newport.The results obtained are as follows; 1. The dry weight of leaf. stem and number of tiller was highest at 22. June and lowest at 21. Aug in alt varieties. And then the dry weight of rhizome, root and thatch increased with growth progressed) Tabte 3). 2. The relationship of the dry weight of biological yield and number of tillers was quadratic ally increased in the growth stage of spring($R^2$= 0.982**), and linearlly increased in the growth stage of autumn(r 0.944*)(Fig. 1). :3. The dry weight of thatch increased as an exponentially equation in alt :3 varieties of Kentucky blue-grass(Fig. 2). Thatch increased rate(TIR) can used to estimate the specifying a quantity of thatch accumulation from the turf. Thatch increase rate equation as follows. where TH$_2$ is the dry weightof thatch at T$_2$ survey time and TH$_1$ is the dry weight of thatch at $TH_1$ is the dry weight of thatch at $T_1$ survey time. $TIR(mg/cm^2/day)=\frac{Ln\;TH_2 - Ln\; TH_1}{T_2 - T_1}$ 4. Correlation coefficients between the dry weight of thatch and leaf weight was -0.633(P>0.05), and number of tiller of tillers was -0.666(P>0.5), respectively. It means that thatch accumu-lation increased with growth depression of leaf and stem.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.130-136
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• 2006

Growth and quality of rice are affected by various factors including the location of cultivation. This study was conducted to investigate the effects of altitudinal locations on the growth and yield-related components of rice. Nineteen Japonica type varieties were grown at Iksan (altitude, 10 m), Imsil (altitude, 150 m), Jinan (altitude, 275 m) and Jangsu (altitude, 430 m) at a similar latitude in Jeonbuk province in the south western Korea. Minimum air temperature showed a strong negative correlation with altitude. The morphological traits and yield-and quality-related components were analyzed. Longer days to heading was required at higher altitudes. However, culm length, panicle length, panicle number, grain number and rice yield were reduced at higher altitudes. Protein content of brown rice increased but fatty acid content decreased at higher altitudes. Amylose content was affected by neither the altitude nor the ecotype. Palatability of polished rice tends to be improved at higher altitudes and in early-maturing ecotypes but its relationships with altitude and ecotype were not significant. Head rice ratio was lower at higher altitudes but broken rice ratio vise versa. These results indicate that growth and quality of rice are affected significantly by changes in temperatures at the locations of different altitude. Also, the characters related to yield and quality of rice often respond incompatibly to the changes in altitudes. These results could provide valuable information for the strategic planning of rice production in geographically diverse areas.

• Journal of Korean Society of Forest Science
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• v.101 no.1
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• pp.158-162
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• 2012

The acclimatization of in vitro propagated plants is an important step to produce vigorous plants for clonal forestry and in vitro micro-environment may affect the growth in ex vitro condition. To monitor in vitro environmental effects on the growth in ex vitro condition, several culture systems such semi-solid medium(SS), temporary immersion bioreactor(TIB) and continuous immersion bioreactor(CIB) culture types were tested to compare for the growth of acclimated plants of Liriodendron tulipifera. Results suggested that morphological characters, stomatal conductance, evapotranspiration and chlorophyll contents of acclimated plants were affected by the different of in vitro culture conditions. CIB type of culture was resulted to the lowest value in the biomass of acclimated plants. Net photosynthsis rate of CIB was the same level as those of SS and TIB. However, stomatal conductance, evapotranspiration and $CO_2$ partial pressure in the intercellular air space were lower than those of SS and TIB. The amounts of chlorophyll a, b and carotenoids were also lower than those of the other two culture systems. TIB, showing a little lower or higher value than SS in many growth character, is recommended rather than CIB to produce healthy yellow poplar plants in ex situ condition.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.307-313
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• 1997

Seed germination test done in laboratory does not coincide with field emergence in general. The experiments were carried out to examine the effect of priming and $GA_3$, treatment to seeds of Platycodon grandiflorum; Codonopsis lanceolata and C. pilosula on lapsed time to first seedling emergence, seedling emergence, morphological characters and growth and the cause of poor emergence of C. pilosula. No-treatment as Control (water), priming or $GA_3$ treatment was done with only distilled water for 2 days, $CA(NO_3)_2$ 150 mM for 2 days or $GA_3$ 0.1 mM for 3 days, respectively. Seedling emergence rate was counted every 2 days but morphological characters and dry weight of shoot and root were measured on 38 days after sowing. Their internal seed structures were examined with Scanning Electron Microscope. C. pilosula had poorer seedling emergence rate than P grandiflorum and C. lanceolata showing nearly same rate: Compared to the other treatment (s) P. grandiflorum displayed higher rate in priming and $GA_3$, treatments but C. lanceolata or C. pilosula did the greatest rate in only $GA_3$ or priming treatment, respectively. $GA_3$ treatment to seeds of P. grandiflorum and C. lanceolata shortened the lapsed time to seedling emergence in comparison with Control, 2-days water imbibition before sowing. In all the species plant height and number of leaves per seedling became shorter and less in priming treatment than the other treatments except plant height of C. Pilosula while their hypocotyl length was nearly same in all treatments. Although priming treatment had nearly similar effect to morphological characters, $GA_3$ treatment forced greater shoot, root and aftermath total dry weight per seedling. Poor seedling emergence of C. pilosula was caused by its seed defect like cleavage or lack of embryo, poor development of embryo and endosperm or their separation.

• Journal of Forest and Environmental Science
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• v.31 no.3
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• pp.214-224
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• 2015

The economy of India and so also of many Asian countries depends on bamboos and their uses are not only in domestic items but also in rural housing and raw materials to several industries and germplasm characterization is an important link between the conservation and utilization of plant genetic resources. Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering coupled with different biotic agencies and environmental factors. Identification and genetic relationships among accessions of Thamnocalamus falconeri were investigated using morphology and random amplified polymorphic DNAs (RAPD) technique. Analysis started by using 51 vegetative characters and forty two 10-mer primers that allowed us to distinguish different genotypes hailing from different eco- zones of Garhwal Himalayas (India). The selected primers (12) were used for identification and for establishing a profiling system to estimate genetic diversity. A total of 79.33% polymorphism was estimated by using 12 selected primers. The genetic similar analysis was conducted based on binary digits i.e. presence (1) or absence (0) of bands, which revealed a wide range of variability among the species whereas genetic relatedness was quite high based on vegetative characters. Cluster analysis clearly showed two major clusters for both of the markers viz. morphology and RAPD belonging to 10 accessions of T. falconeri. Two major clusters were further divided into minor clusters. Cluster based on RAPD marker showed grouping of accessions of closed locality whereas analogy was reported for vegetative traits. The RAPD technique has the potential for use in species identification and genetic relationships studies of bamboo for breeding program.

• Journal of Mushroom
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• v.11 no.1
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• pp.1-8
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• 2013

In this study, twelve of Lepista nuda were collected from various localities in Korea. Also thirteen exotic L. nuda species were collected from Japan, France, Switzerland and Portugal. Spores were isolated under optical microscope. These spores were placed on the surface of YM medium for inducing to germination. Eleven mating-groups were selected by morphological characters of fruit body such as size, color and stipe patterns. Intra-isolate crosses were made between two single-spore isolates derived from mating-groups. Also, dikaryotic crossing using the isolates from L. nuda were carried out to evaluated tetrakaryon formation. Cross-mating compatibility tests also verified its dikaryotic state by microscopic or molecular genetic observation of clamp connection and Random Amplified Polymorphic DNA (RAPD) band pattern. To analyze the growth rate of hybrids and parents mycelium in dikaryons obtained from compatible mating groups were placed on PDA medium. Intra-isolate crosses determined eleven mating-groups within L. nuda. The typical clamp connection were mostly observed in mating-groups of Korean L. nuda in $K1{\times}K2$, $K1{\times}K3$, $K1{\times}K4$, $K1{\times}K6$, $K1{\times}K5$, $K2{\times}K4$, $K2{\times}K3$, $K2{\times}K6$, $K3{\times}K4$, $K4{\times}K5$, and $K4{\times}K6$. Korean L. nuda type of dikaryon, shown to cross-incompatibility with L. sordida, it seemed that mating induce more rapidly than wild types in a view of growth rate. In conclusion, it would be useful to improve mass production with better morphological characteristics through a special mating of L. nuda.

• The Plant Pathology Journal
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• v.33 no.6
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• pp.543-553
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• 2017

Sesame (Sesamum indicum) is an important oil seed crop of Asia. Yields can be negatively impacted by various factors, including disease, particularly those caused by fungi which create problems in both production and storage. Foliar diseases of sesame such as Alternaria leaf blight may cause significant yield losses, with reductions in plant health and seed quality. The work reported here determined the incidence of Alternaria species infecting sesame seeds grown in the Punjab, Pakistan. A total of 428 Alternaria isolates were obtained from 105 seed samples and grouped into 36 distinct taxonomic groups based on growth pattern and morphological characters. Isolation frequency and relative density of surface sterilized and non-surface sterilized seeds showed that three isolates (A13, A47 and A215) were the most common morphological groups present. These isolates were further identified using sequencing of the Internal Transcribed Spacer (ITS) region of ribosomal DNA (rDNA) and the Alternaria major allergen gene (Alt a 1). Whilst ITS of rDNA did not resolve the isolates into Alternaria species, the Alt a 1 sequences exhibited > 99% homology with Alternaria alternata (KP123850.1) in GenBank accessions. The pathogenicity and virulence of these isolates of Alternaria alternata was confirmed in inoculations of sesame plants resulting in typical symptoms of leaf blight disease. This work confirms the identity of a major source of sesame leaf blight in Pakistan which will aid in formulating effective disease management strategies.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.61-70
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• 2020

A eukaryotic microalga was isolated from seawater in Janghang Harbor, Korea and its morphological, molecular, and physiological characteristics were investigated. Due to its simple morphology, no distinctive characters were found by morphological observation, such as light microscope or scanning/transmission electron microscopy (S/TEM). However, molecular phylogenetic evidence inferred from the concatenated small subunit (SSU) 18S rRNA and internal transcribed spacer (ITS) sequence data indicated that the isolate belonged to the newly described Micractinium singularis. Furthermore, it was clustered with Antarctic Micractinium strains and it also showed a psychrotolerant property, surviving at temperatures as low as 5℃. However, its optimal growth temperatures range from 15℃ to 25℃, indicating that this microalga is a mesophile. Additionally, gas chromatography-mass spectrometry (GC/MS) analysis showed that the isolate was rich in nutritionally important omega-3 polyunsaturated fatty acid, and high-performance liquid chromatography analysis (HPLC) revealed that the high-value antioxidant lutein was biosynthesized as an accessory pigment by this microalga, with glucose as the major monosaccharide. Therefore, in this study, a Korean marine M. singularis species was discovered, characterized, and described. It was subsequently added to the national culture collections.

• Research in Plant Disease
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• v.26 no.2
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• pp.109-115
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• 2020

A disease causing leaf blight and vine rot was recognized on kudzu plants (Pueraria lobata) in Korea since 1991. A species of Phytophthora has been repeatedly isolated from the infected leaves. Identification in species level of the Phytophthora sp. remained unsolved. An isolate, KACC 47616 originally collected from Manchon Park in Daegu, has been kept in our laboratory. In 2013, three new isolates, KACC 47617 and KACC 47618 from Yeongyang and KACC 47619 from Gunwi in Gyeongbuk province, were collected and examined to classify up to species level by characterizing morphology, response to temperature and phylogenetic relationship. On the basis of morphological characters such as the nature of hyphal swelling, sporangia and sex organs, absence of chlamydospore production, optimum temperature for mycelial growth, and internal transcribed spacer rDNA and cytochrome oxidase subunit 1 sequence analysis of the pathogen, the causal fungus of kudzu plant was identified as Phytophthora asiatica.