• Development and Reproduction
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• v.16 no.4
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• pp.329-338
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• 2012

The proliferation of embryonic cells or adult stem cells in tissue is critically regulated during development and repair. How limited the proliferation of cells, so far, is not much explored. Cell-cell contact proliferation inhibition is known as a crucial mechanism regulating cell proliferation in vitro and in vivo. In this study we examined the characters of mouse subcutaneous adipose derived stem cells (msADSC) whether they lost or get contact inhibition during in vitro culture. The characters of msADSC growth after confluence were analyzed using confocal microscope and the expression profiles of contact inhibition related genes were analyzed according to the morphological changes using real-time PCR method. msADSC showed overlapping growth between them but not after passage 14. The cell shapes were also changed after passage 14. The expression profiles of genes which are involved in contact inhibition were modified in the msADSC after passage 14. The differentiation ability of msADSCs to adipocyte, chondrocyte and osteocyte was not changed by such changes of gene expression profiles. Based on these results, it is revealed that smADSC were characterized by getting of strong cell-cell contact inhibition after passage 14 but the proliferation and developmental ability were not blocked by the change of cell-cell contact proliferation inhibition. These finding will help to understand the growth of adipose tissue, although further studies are needed to evaluate the physiological meaning of the cell-cell contact proliferation inhibition during in vitro culture of msADSC.

• 한국해양학회지
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• v.13 no.2
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• pp.26-30
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• 1978

This work concerns morphological studies of Alaska pollak, Theragra chalcogramma(PALLAS), and of the Nogari which is generally known as an immature form of the Alaska pollack. Observations are made to see whether significant differences in the meristic and morphometric characters can be recognized between two groups. Four meristic characters, namely, first anal fin rays, first dorsal fin rays, gill rakers, vertebra and three morphometric characters, namely, head length, snout length, eye diameter are chosen for the study. Results of the analyses reveal that the differences in all the selected meristic characters between Nogari and Alaska pollack are found to be not significant. Differences in the snout length in relation to the head length are found to be not significant . However, the differences in the head length in relation to the body length and the differences in eye diameter in relation to the head length are both found to be significant. In spite of the lack of informations on genetypic variations, it is reasonable to state that the Nogari appears to be a juvenile form of the Alaska pollak, and the differences in certain morphometric measurements between two groups are likely to be associated with the differences in the relative growth rates during the life stages.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.23-23
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• 2023

The Hairy labrador tea (Ledum palustre L. var. diversipilosum Nakai) is a evergreen small shrub, belonging to the Ericaceae and mainly distributed along the alpine areas of Hamgyeong-do in North Korea. Through seed physiological research on North Korean plants, we intend to obtain basic data for the development of mass propagation methods and use them for vegetation restoration. The internal and external morphological characters of the seed were observed using scanning electron microscopy or stereoscopy, and full seeds were selected through X-ray test. Seeds were cultured on a growth condition at 15/6℃ and 25/15℃ to examine the germination response according to temperature, and the number of germinated individuals was examined every 24 hours to calculate germination percentage and mean germination time. Low-temperature treatment (4℃) and hormone treatment (GA3) were performed to investigate the effect of dormancy breaking, and analysis of variance (ANOVA) was performed on the experimental results using SAS 9.4. The seeds of the Hairy labrador tea were elliptical-narrow in shape, and the epidermal cell wall pattern was reticulated. The final germination rate (FGP) was the highest at 58.9% under the condition of low temperature treatment at 25/15℃. The average number of days to germination (MGT) was the highest at 25.1 days without treatment at 15/6℃, and the average of the last germination days was confirmed to be about 13 days. As a result of low temperature treatment, it was confirmed that the final germination rate (FGP) increased by 43.3%. The significance of the final germination rate (FGP) was verified according to the pretreatment at 25/15℃.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.211-215
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• 1983

Thirty eight varieties with high uniformity and clearance in grain quality among 70 colored soybeans collected from nation-wide provinces were investigated for their seed morphological and agronomic traits. As for seed morphological traits, grain volumetric size, grain weight, grain length, grain thickness, . hilum length and width were studied on the tendency of statistical dispersion and variations. As a result, four groups could be classified on the basis of 100 grains weight, so as small, medium, large, and super large groups, Also, as for agronomic traits, among others, the tendency of setting in flowering date, maturing date, yield components, and stem weight, stem length were studied on the basis of statistical inter-relationships between above mentioned characteristics. From the above, bigger variations were detected in weighing characters of soybean plants than in duration characters. And the flowering and maturing days showed significant relationships to the stem length, stem weight, also stem length and stem weight to the grain yield.

• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.525-530
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• 1998

These studies were carried out to select the active grow hairy root lines induced from various ginseng(Panax ginseng C. A. Meyer) parts. Hairy roots were induced in root explants, stem and petiole in vitro by A. rhizogenes R1000 or A. rhizogenes $A_4$. These hairy roots could be grown on the phytohormone free medium, and PCR analysis of rol C and vir C gene fragments confirmed that hairy roots were transgenic tissues. We have selected 11 hairy root lines with active growing characters among 300 hairy root lines selected based on growth and morphological characteristics on 1/2MS solid media with 250 mg/L carbenicillin. Morphological characteristics of selected 11 hairy root lines were thickness and thiness of main roots, and many projection for lateral roots, active grow of lateral roots. Among selected 11 hair root lines prominent characteristics of hairy roots with active growing characters were thiness of main roots and active grow of lateral roots. But characteristics of low growing hairy roots were thickness of main roots and low grow of lateral roots. Finally we have selected actively growing hairy roots, KGHR-1, KGHR-5, KGHR-8 among 11 hairy root lines.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.431-438
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• 2010

Anthers of sweet pepper $F_1$ cultivar 'Special' were cultured on Dumas De Vaulx (C medium), supplemented with $0.1mgL^{-1}$ 2, 4-D and $0.1mg{\cdot}L^{-1}$ kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and $9.8^{\circ}Brix$, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.

• Journal of Plant Biology
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• v.8 no.1_2
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• pp.11-18
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• 1965

Since Taquet's first discovery of wild P. yedoensis at Mt. Halla, Korea, in 1908, its morphological chracteristics and question of origin of cultivated yedoensis have given controversies to the botanists. Takenaka, through his experiments on the hybridity of cultivated P. yedoensis, recently holds the opinion that P. yedoensis might have originated in Izu peninsula, Japan. The author presents the summarized report on the wild P. yedoensis and its putative parents based on his 2 years' investigated carried out at Mt. Halla during his breeding experiment of genus Prunus. The species of cherry tree used in the present investigation were identified by Prof. Mankyu Park and Mr. Jonghyu Pu, Korean taxonomists. 1) Wild cherry trees which grow wild in Mt. Halla and whose blooming season is April are mostly P. subhirtella var. pendula form. ascendens and P. donarium P. yedoensis is rare in number, around 10 individuals, having been found in a half century. 2) Individuals of wild yedoensis are variable in some of their morphological characteristics. This is, also, true in other species of Prunus. 3) Wild yedoensis whose vigorous growth, sterility, and rarity in number suggest hybrid origin, has intermediate characteristics between the P. subhirtella and P. donarium. 4) Due to the abnormal weather of the island and various environmental factors such as havbitats, some of the early-blooming subhirtella and late-blooming donarium would bloom at the same time, giving these two species the changes to cross. 5) Wild yedoensis is slightly different in some of its quantitative characters from cultivated species.

• Journal of Environmental Science International
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• v.21 no.11
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• pp.1297-1305
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• 2012

Clonal plants combine sexual and clonal reproduction, which contribute differently to plant fitness. Reproductive analyses have highlighted the importance of clonal growth in shaping the spatial genetic structure in Potentilla conferta Bunge, a herbaceous rhizomatic clonal distributed in hot sand dunes. We investigated the reproduction system of P. conferta at two populations in Mongol and three natural populations in Uzbekistan. The measurements of 19 quantitative or qualitative morphological characters were taken on each of total individuals directly from their natural habitats. Some morphological characteristics between Mongolia and Uzbekistan populations showed a slight heterogeneity of variance. Especially, the length of internodes (LFI and LSI) and characteristics of root (LLR and NOR) were shown a significant difference between two countries (P<0.05). P. conferta of Uzbekistan has most ramets at short distance intervals 30~100 cm. In light conditions, P. conferta of Uzbekistan was significantly less resilience than P. conferta of Mogol. In drought conditions, although there was not shown significant difference, P. conferta in Uzbekistan was less resilience than that in Mogol. The core analysis indicates that P. conferta in Uzbekistan is the more resistant than that of Mongol and seems to do by sexual reproduction strategy during several strong environmental disadvantages such as drought events.

• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.259-263
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• 2003

This study was carried out to get basic information about the morphological and production characteristics of fennel populations different origin under different ecological conditions in Hungary. The Korean population can be registered as a medium high genotype and showed a more accelerated growth characters than Hungarian taxon and the two populations have a medium homogeneity $(CV\;=\;4{\sim}22%)$. In the second year plant height in Hungarian taxon was significantly higher than that of the Korean population. The shooting dynamic of individual plants might be heterogeneous in both taxa and the Korean population was not only shorter in the height but had also less shoots by 16% than the Hungarian one. The Hungarian taxon exceeded the umbel size and numbers to the Korean one (by 3.62 cm in the average) and the variability in the populations was high $(CV\;=\;29{\sim}49%)$. Seed size was proved to be also a discriminative feature between the examined taxa. With small deviations (CV < 2) the Hungarian population produced seeds longer by 38%. Homogeneity in the populations was dependent more on the vegetation years and on the characteristics measured but less on the origins, respectively.

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3397-3406
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• 2012

Three types of spherulitic morphologies have been investigated in dual substrate mode of Belousov-Zhabotinsky (BZ) type reaction system. Prior to growth of spherulites, three distinct patterning behaviors have been observed sequentially during the reaction process. Initial and the early-phase of reaction showed the emergence of concentric ring-like wave patterns. A colloidal-state of reaction consists of numerous fine solid particles, which forms primarily some nucleation centers of dendritic characters. The nucleation centers were found to grow in sizes and shapes with the progress of reaction. It leads to growth of dendritic-like spherulitic crystal patterns. The resultant spherulites showed transitions in their morphologies, including sea-weeds and rhythmic spherulitic crystal patterns, by the effects substituted organic substrate and in the higher concentration of bromate-initiator respectively. The branching mechanism and crystal ordering of spherulitic textures were studied with help of optical microscope (OPM) and scanning electron microscope (SEM). Characteristics of crystal phases were also evaluated using X-ray diffraction (XRD) and differential thermal analysis (DTA). Results indicated that the compositions of reactants and crystal orderings were interrelated with morphological transitions of spherulites as illustrated and described.