• Title/Summary/Keyword: growth and activities

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Physiological and Ecological Characteristics of Indigenous Soybean Rhizobia Distributed in Korea -II. Studies on Some Physiological Characteristics and Nitrogen Fixation Activity Under Free-Living Conditions of Indigenous Rhizobia (우리나라 토착대두근류균(土着大豆根瘤菌)의 분포상태(分布狀態)와 생리(生理) 및 생태학적(生態學的) 특성(特性) -제(第)II보(報) : 토착근류균(土着根瘤菌)의 질소고정력(窒素固定力)과 생리적특성(生理的特性)에 관(關)한 연구(硏究))

  • Ryu, Jin-Chang;Lee, Seong-Jae;Suh, Jang-Sun;Cho, Moo-Je
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.2
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    • pp.157-165
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    • 1986
  • This experiment was conducted to find out the some physiological characteristics and nitrogen fixation activities under free-living conditions of indigenous rhizobia isolated from soybean-cultivated (30 fields) and un-cultivated (30 fields) soil. The results were summerized as follows: 1. In free-living condition, only 12.8% and 6.4% of the indigenous rhizobia isolated from soybean cultivated (133 strains) and un-cultivated (125 strains) soils were nitrogenase positive as more than 4 n mole $C_2H_4$ per tube per hour by acetylene reduction assay. 2. The acid-producing rhizobia on litmus milk test was observed to be 20% of the total 160 strains isolated from soybean cultivated soil but about 34% of 166 strains isolated from un-cultivated soil. And the serum zone positive strains were higher in the soybean un-cultivated soil than cultivated soil. 3. The population ratio of fast-to slow-growing indigenous rhizobia based on growth pattern of AMA medium was 35.6% to 64.4% of the total 346 strains. 4. The population of indigenous Rhizobium japonicum counted by MPN method was ranged from $9.2{\times}10^2$ cells per gram of soil in soybean un-cultivated soil to $2.3{\times}10^4$ cells per gram of soil soybean cultivated soil. The number of indigenous R. japonicum in 0-10cm depth of surface layer was higher than low layer.

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Studies on the Use of Radioisotope Tracer Technique to Investigate and Improve the Root Activities in Rice Plant (II) - Effect of Application of Several Kinds of Phosphorous Fertilizer - (방사성동위체(放射性同位體) 도입(導入)과 그 추적기술(追跡技術)에 의(依)한 수도근계(水稻根系) 활성상(活性相)의 해명(解明)과 개선(改善)에 관(關)한 연구(硏究) - 인산질(燐酸質) 비료(肥料)의 비종별(肥種別) 시용효과(施用效果)에 대(對)하여 - (제2보)(第2報))

  • Ahn, Hak-Soo;Chung, Hee-Don;Ahn, Jon-Sung;Ro, Jun-Chong;Kim, Kyu-Won;Shim, Sang-Chil
    • Applied Biological Chemistry
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    • v.15 no.1
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    • pp.85-92
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    • 1972
  • The field experiment was performed to investigate the effects of various kinds of phosphorus fertilizers such as double superphosphate, fused magnesium phosphate and Simagcarin (both the Kyun-gi Chemical Co, products) on the physiological roles in development of root system, growth and yield compositions of rice plant. Radioactive phosphoric acid $(H_3\;^{32}PO_4)$ was applied to measure the root activity. 1. The number of total tillers was significantly increased in double superphosphate plots, but the rate of fruitful tillers was more numerous in the fused magnesium phosphate and the Simagcarin plots than that of the other plots. 2. The grain yield was much more obtained in the fused magnesium phosphate and Simagcarin plots (no significant difference were found between both of plots) than the double superphosphate and control plots. It seemed due to the increasing of seedbearing rate and number of fruitful tillers. 3. In double superphosphate plots, root system was mostly developed near topsoil areas, but fused magnesium phosphate and the Simagcarin plots, root system was uniformly distributed from topsoil to subsoil areas. 4. As the results of those experiments, fused magnesium phosphate and Simagcarin was demonstrated to be soil amendmentical materials rather than the phosphorus fertilizers, especially in low productive paddy soils which lack the special mineral nutritions.

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A Case Study for Developing 'Personal Practical Knowledge(PPK)' of Pre-service Chemistry Teachers: Based on the Reflective Discussion of Community of Practice(CoP) Activity (예비 화학교사들의 실천적 지식(PPK) 함양을 위한 사례연구 -실행공동체(CoP) 활동의 반성적 논의를 중심으로-)

  • Kim, Yu-Jung;Hong, Hun-Gi
    • Journal of The Korean Association For Science Education
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    • v.37 no.2
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    • pp.347-358
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    • 2017
  • The purpose of this study is to research on the professional development of pre-service chemistry teachers in terms of personal practical knowledge(PPK), and draw a discussion for pre-chemistry teacher education. A reflective discussion through a Community of Practice(CoP) activity has been conducted with 5 pre-service chemistry teachers studying in a college of education located in Seoul. The analysis of the results reveals that 'Subject-matter knowledge' is leaning toward massive chemical contents, 'Curriculum knowledge' is below the level of selecting, analyzing, and applying curriculum materials. 'Instruction knowledge', especially the knowledge of chemistry experiment and research, is not enough to lead secondary students' scientific experiment. 'Milieu of school knowledge' is stunted in growth for the reason of communication-deficiency between a college of education and a community, and among education- related groups. 'Self-knowledge' has been diminished not only because there is a bad influence from college classmates not wanting to be a teacher, but also a lack of communication in the chemistry-education department which can make pre-teachers' belief strong. In conclusion, it is especially needed for the chemistry-education department to strengthen pre-teachers' 'Self-knowledge' in order to develop PPK. This study suggests operating 'a professional community' that has a periodic meeting with pre-service teachers, teachers, professors, community people, education practitioners, and researchers. In addition, it would be effective for increasing pre-teachers' PPK to reconsider depth and direction of 'Subject-matter knowledge', to give an opportunity to examine curriculum materials critically and reorganize them, to improve the course of chemistry experimental subject to the way of improving pre-teachers' leadership in chemistry inquiry experiment, and to raise the quality of educational service activities.

Macrophage and Anticancer Activities of Feed Additives on β-Glucan from Schizophyllum commune in Breast Cancer Cells (치마버섯균 유래의 베타글루칸에 대한 사료첨가제로서의 대식세포 기능 활성 및 유방암 세포주에서의 항암효능 효과)

  • Lee, Jin-Seok;Lee, Seung-Ho;Jang, Yong-Man;Lee, Jong-Dae;Lee, Byoung-Hee;Jung, Ji-Youn
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.949-955
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    • 2011
  • [ ${\beta}$ ]Glucan is a polysaccharide expressed on the cell walls of fungi. It is known that ${\beta}$-glucan is recognized by a family of C-type lectin receptors, dectin-1, which is expressed mainly on myeloid immune cells, including macrophages, neutrophils and dendritic cells. Raw 264.7 cells were treated with ${\beta}$-glucan from Schizophyllum commune. ${\beta}$-Glucan was not cytotoxic up to 400 ${\mu}g$/mL as measured by MTT assay. To measure the activity of macrophages, NO and TNF-${\alpha}$ assays were performed in Raw 264.7 cells. Treatment with ${\beta}$-glucan for 24 hr significantly increased production of NO and TNF-${\alpha}$ compared with control groups (p<0.05), indicating activation of macrophages. To measure inhibition of breast cancer cell proliferation, MTT assay was performed in MDA-MB-231 cells. Cell viability was significantly decreased in the group treated with 400 ${\mu}g$/mL of ${\beta}$-glucan for 48 hr (p<0.05) compared to the control group. However, tumor volume was decreased in the groups administered 200 ${\mu}g$ of ${\beta}$-glucan/mouse compared to the control group. These results indicate that ${\beta}$-glucan inhibits breast cancer cell growth through the induction of apoptosis.

Tracing the Development and Spread Patterns of OSS using the Method of Netnography - The Case of JavaScript Frameworks - (네트노그라피를 이용한 공개 소프트웨어의 개발 및 확산 패턴 분석에 관한 연구 - 자바스크립트 프레임워크 사례를 중심으로 -)

  • Kang, Heesuk;Yoon, Inhwan;Lee, Heesan
    • Management & Information Systems Review
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    • v.36 no.3
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    • pp.131-150
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    • 2017
  • The purpose of this study is to observe the spread pattern of open source software (OSS) while establishing relations with surrounding actors during its operation period. In order to investigate the change pattern of participants in the OSS, we use a netnography on the basis of online data, which can trace the change patterns of the OSS depending on the passage of time. For this, the cases of three OSSs (e.g. jQuery, MooTools, and YUI), which are JavaScript frameworks, were compared, and the corresponding data were collected from the open application programming interface (API) of GitHub as well as blog and web searches. This research utilizes the translation process of the actor-network theory to categorize the stages of the change patterns on the OSS translation process. In the project commencement stage, we identified the type of three different OSS-related actors and defined associated relationships among them. The period, when a master commences a project at first, is refined through the course for the maintenance of source codes with persons concerned (i.e. project growth stage). Thereafter, the period when the users have gone through the observation and learning period by being exposed to promotion activities and codes usage respectively, and becoming to active participants, is regarded as the 'leap of participants' stage. Our results emphasize the importance of promotion processes in participants' selection of the OSS for participation and confirm the crowding-out effect that the rapid speed of OSS development retarded the emergence of participants.

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The influencing effect on E.Q. and personality that both sports activity & speciality aptitude activity in school-childhood can cause (학동기의 스포츠활동과 특기적성활동의 참가가 감성지수 및 성격특성에 미치는 영향)

  • Lee Han-Ki
    • The Journal of Korean Physical Therapy
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    • v.16 no.1
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    • pp.140-156
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    • 2004
  • This study, to find whether a sports activity and a speciality aptitude activity in school-childhood can affect in forming E.Q, has been done in Gyoung-Nam area and Busan wide city with asked 222 of men and women being in their school-childhood and a group of 85 people who had not joined in such activity, using a E.Q testing paper provided by Dae-Gyo Education Corp,. and Seoul National University Educational Research Institute. Following is the results after analyzing the compiled datas. 1. The E.Q. level difference between people who joined, and who not joined in a sports action activity was reported existing, the total E.Q average of those who joined was resulted 212.6, a point 29.6 higher than those not joined of 183.0 ( p< .05). As for the E.Q causing points, it resulted that the points of the joined group is generally up than that of the non-joined group, especially this difference was remarkable in terms of feeling recognition or feeling control, a finding that deserves an attention ( p<.05) 2. Joining periods of sports activity did also have relation to develping E.Q. of school-childhood according to this research, the total E.Q points of a group joined in the activity more than 2 years was 215.5 points, which was 17.4 points higher than those not joined of 186.5 points ( p< .05). Backing again to E.Q causing points in this case, it resulted without exeption of all main causes that those who joined in more than 2 years are generally higher than that of those joined less than 2 years, especially the difference was regarded as big in terms of feeling recognition or feeling control, a finding that deserves an attention ( p<.01). 3. The E.Q. differnce between those joined in a specialty aptitude activity and not joined was studied existing, the total E.Q average points of those joined in a specialty aptitude activity was 207.8, a higher figure by 21.3 points than those not joined group of 186.5 ( p< .05). As for the E.Q causing points, it resulted without exeption of all main causes that those who joined are generally higher than that of those not joined, especially for feeling recognition or feeling control, this difference was more clear, a finding that deserves an attention ( p<.01). 4. It also resulted that E.Q growth depends on the periods to have joined in a speciality aptitude activity, for example, the total E.Q points of those joined in the activity more than 2 years was 217.1, a total more higher by 13.5 points than 203.6 of those not joined ( p< .05). For the E.Q. causing points, it, with the exception of empathy was resulted that those who joined in the speciality aptitude activity more than 2 years are generally higher than those joined less than 2 years, especially the difference is remarkable in terms of feeling recognition or feeling control, a finding that is also remarkable ( p<.05). 5. The E.Q difference between the men and women who joined in both activities of sports & speciality aptitude was found existing, the total E.Q. average for women was resulted 214.2 points, which was 9.2 points higher than men of 205.0. As for the E.Q. causing points, which, without exeption of main causes, women's was reported being high than that of men, in special is more remarkable in terms of feeling control, a finding that deserves an attention. ( p<.05).

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Antibacterial and Antibiofilm Activities of Leaf Extracts of Stewartia koreana against Porphyromonas gingivalis (Porphyromonas gingivalis에 대한 노각나무 잎 추출물의 항균활성 및 생물막 형성 억제 효과)

  • Kim, Hye Soo;Park, Min Jeong;Kim, Soo Jeong;Kim, Bu Kyung;Park, JunHo;Kim, DaeHyun;Cho, Soo Jeong
    • Journal of Life Science
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    • v.31 no.3
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    • pp.330-337
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    • 2021
  • This study was conducted to investigate the potential of Stewartia koreana as oral healthcare materials. The antibacterial activity of ethanol extracts from leaves and branches of S. koreana against oral bacteria was confirmed. The leaf and branch extracts (1 mg/disc) showed antibacterial activity against P. gingivalis only among several tested oral bacteria. The leaf extracts showed higher antibacterial activity, with values similar to those of chlorhexidine, which was used as a positive control. The MIC of the leaf extract against P. gingivalis was 0.4 mg/ml and showed bacteriostatic action. The inhibitory effects of the extract on biofilm formation and on gene expression related to biofilm formation by P. gingivalis were determined by biofilm biomass staining, scanning electron microscopy (SEM), and qRT-PCR analysis. The biofilm production rate and cell growth of P. gingivalis in the cultures treated with 0.2-2.0 mg/ml of S. koreana leaf extracts were significantly decreased in a concentration-dependent manner. The inhibitory effect on the formation of P. gingivalis biofilms at concentrations of 1 mg/ml was confirmed by SEM. The qRT-PCR analysis showed concentration-dependent suppression of the fimA and fimB gene expression associated with fimbriae formation in the cultures treated with 0.2-2.0 mg/ml S. koreana leaf extract. These results support the conclusion that S. koreana leaf extracts can be used as oral healthcare materials derived from natural materials, as demonstrated by the antibacterial action and inhibition of biofilm formation of P. gingivalis.

Relationship between Compulsory Employment System for persons with Disabilities and Health Status (장애인 의무고용제도와 건강상태 간의 연관성)

  • You, Jin Ha;Kim, Ye Won;Yang, Jeong Min;Kim, Jae Hyun
    • Korea Journal of Hospital Management
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    • v.27 no.2
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    • pp.53-66
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    • 2022
  • Aim : The purpose of this study is to investigate the influence of the awareness level of the mandatory employment system for the disabled and the help of the employment increase on the health status of the disabled. Based on this, the purpose of this study is to raise awareness of the compulsory employment system for the disabled and to suggest a way to improve the health of the disabled. Methods : In this study, the first data of the second wave of the 2016-2018 Employment Panel Survey for the Disabled was used to analyze the participation of the disabled in economic activities. a total of 1,648 subjects were analyzed using the chi-square test and generalized estimating equation (GEE). Results : Compared to the group knowing about the compulsory employment system for the disabled, in the group who did not know about the compulsory employment system, the cognitive level was significantly higher for subjective health status (Odds Ratio [OR]: 1.573, 95% Confidence Interval [CI]: 1.252-1.977) and chronic diseases (OR: 1.407, 95% CI: 1.091-1.816), Compared to the group that the compulsory employment system for the disabled is helpful in increasing employment, the group that said it was not helpful at all had depression feel (OR: 2.330, 95% CI: 1.219-4.452) and subjective health status (OR: 2.052, 95% CI: 1.232. -3.416) significantly affected. Conclusion : It was found that the lower the level of awareness and help of the compulsory employment system for the disabled, the negative impact on their health status. Therefore, it is necessary to promote employment growth by raising awareness of the employment system for the disabled. Furthermore, in order to improve the health of the disabled, public relations plans to increase the understanding of the disabled on the system and policies to protect the disabled people who lose their will to work due to difficulties in accessing employment were enacted, and Changes to a disability-friendly working environment should be activated so that participants can participate.

Determination of Types and Element on Parking Ramp (주차장 램프 형식 결정 및 제원 산정에 관한 연구)

  • Kwon, Sung-Dae;Kim, Yoon-Mi;Nam, Chang-Kyu;Ha, Tae-Jun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.5
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    • pp.2021-2031
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    • 2013
  • Due to the rapid economic growth within the nation, the quality of life of individuals have improved dramatically. The scope of living activities of individuals have also extended, resulting in a rapidly increasing demand for automobiles. The number of vehicles registered in Korea is rapidly increasing and will reach 188.71 million as of December 2012. Compared to the registered residents of 50.94 million provided by the Ministry of Public Administration and Security, the registered population reflects about 4 people per every automobile. Due to the high demands for vehicles, the demands for parking lots in collective housing and businesses are also increasing. In reality, the current state of expansion of parking lots are underground, due to the limited available space on ground level. Specifically, the slope of a parking lot cannot exceed 17% linear slope and 14% curved slope according to the 'parking lot laws', however studies show that the driver feels at risk for safety when stopped on the parking ramp while driving in the parking lot. This study seeks to examine the suitability of parking lot ramps, concerning the safety aspects of the driver. First, the ramp type was categorized as linear or curved, then test drives were performed based on variations of slopes, slant distances, directions and points. A survey was administered to the driver after the completion of the test drive, in order to element design for an ideal ramp. In the case of curved ramp, the results of the estimate suggests a counterclockwise, slope at a maximum of 12% incline. The maximum slope for a linear ramp was analyzed to be between 13~14%, suggesting that slope greater than 15% need to be eliminated. In conclusion, it is anticipated that the element design parking ramp reported in this study will help to serve as a reference for future parking lot related guidelines, and provide cost effective traffic safety mechanisms in future parking lot businesses to follow.

Anti-inflammation Activities of Cultured Products from Suspension Culture of Aloe vera Callus (Aloe vera Callus 현탁배양 생성물의 항염증 활성)

  • Kim, Myung Uk;Cho, Young Je;Lee, Shin Young
    • Journal of Applied Biological Chemistry
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    • v.56 no.3
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    • pp.157-163
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    • 2013
  • Cultured products (callus and exopolysaccharide) were obtained from suspension culture of Aloe vera callus, and the extracts of callus were further prepared with cold water or 60% ethanol solution. The ethanol extract of callus (AC) and exopolysaccharide (ACP) of 10 mg/mL exhibited the relatively higher suppression activity of 43.2-52.1% against hyaluronidase activity. Thus, their anti-inflammatory effects were further investigated using animal cell (Raw 264.7) in vitro. Though AC shows a slight suppression effect of cell survival rate (97%) using MTT assay in the presence of $400{\mu}g/mL$ AC- dimethyl sulfoxide (DMSO), cell growth promotion was observed in the other samples of lower levels. It indicates that the ethanol extract of Aloe callus rarely affect cell survival rate in the ranges ($200-400{\mu}g/mL$) used in the study. Using Griess reagent, the suppression of NO production by the aloe callus extract was analyzed by measuring the amount of the nitrite produced in Raw 264.7 culture activated by lipopolysaccharide (LPS). As a result, supplementation of AC-distilled water (DW) and AC-DMSO produced higher levels of NO than the positive control LPS. However, the NO suppression effect by ACP-DW was so intense that lower amount ($80-100{\mu}g/mL$) suppressed NO production to the level of the control. The effect was attributed to the expression of the iNOS. Then, Raw 264.7 cells were stimulated with the LPS and expression of COX-2 protein level was analyzed depending on the Aloe suspension culture product treatment. The results showed that the ACP-DW supplemented medium did not express COX-2 by itself, and LPS stimulated COX-2 expression was slightly decreased. On the other hand, realtime-PCR analysis of the expression of inflammatory cytokine showed that IL-$1{\beta}$ and TNF-${\alpha}$ expression was highly suppressed in the ACP- distilled water supplemented medium.