• Title/Summary/Keyword: growing stages

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Comparison of Some Propagation Methods in Paeonia albiflora Pallas and Effective Components of Paeonia albiflora Pallas Root Grafted with Paeonia moutan Sims (작약의 번식방법과 작약 / 목단 접목근의 유효성분 비교)

  • 이희덕
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.3
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    • pp.283-287
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    • 1992
  • This experiment was carried in order to compare some propagation methods and the effective components in the root of Paeonia albiflora pallas, Paeonia moutan Sims and Paeonia albiflora pallas root grafted with Paeonia mouton sims The optimum seeding time of August 20 to september 10 for increasing germination ratio. The growing and developing of Paeonia albiflora Pall. for root dividing methods was advantageous to practice at Autum than at spring season. The shoot cutting method during the flowering time was proper to increase the rooting ratio with the aid of Ruton, the plant growth regulator but not emerged of apical bud. The grafting stages between the Paeonia albiflora pall. and Paeonia moutan Sims was available to practice during the September for increasing the thickness growth of root. Among the effective components of the paeony the paeoniflorin was shown high, while paeonol in paeony moutan. The paeoniflorin of herbaceous paeony / paeony moutan was increased about 75.9% than herbaceous paeony alone, and the other compoents are similar to those of herbaceous paeony root.

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Ovulation Rate and Early Embryonic Development of Mouse Atretic Follicular Oocytes Induced by High-dose Gonadotropin (과량의 생식소자극호르몬 처리를 받은 생쥐 폐쇄난포의 배란율과 초기배아 발생률의 변화)

  • 임천규
    • Development and Reproduction
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    • v.1 no.1
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    • pp.67-77
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    • 1997
  • Mammalian ovary consists of various growing stages of follicles. Ovarian follicular growth and differentiation, however, can be distinguished into recruitment, growth, selectiona nd ovulation. while only minute of the selected follicles ovulate their oocytes, all the rest follicles disappear by atresia. this atresia is an important event of which physiological mechanism must be resolved. The present study was carried out to investigate the effects of various doses of pregnant mare's serum gonadotropin (PMSG) on the oocyte quality, ovulation rate, and the early embryonic development in immature mice. Immature mice were administrated with 5, 20, or 40 IU PMSG. At every 12 hour up to 72 hour after treatment, body and ovary weights were measured. Oocytes were flushed from the oviducts under the dissecting microscope and observed under the inverted microscope. Late 2-cell embryos were collected from the mice which were superovulated by the same dosage of PMSG followed by 5 IU hCG 47 hours after PMSG-treatment. The percentage of abnormal oocytes was higher in 20 or 40 IU PMSG-treated animals than 5 IU PMSG-treated ones. Ovulation occured at 12 hours afger PMSG injection in all experimental groups. The percentage of retrieved abnormal oocytes increased in the 20 or 40 IU PMSG-treated goups but not in 5 IU PMSG-treated group. There was no significant difference in the mating rate among the groups [52.6% (10/19), 66.7% (10/15), 44.0% (11/25) : 5, 20, 40 IU group respectively] ; however, ther was a significant (p<0.01) increase of embryo retrieval rates in 5 and 20 IU-treated groups compared with that in 40 IU-treated group [89.2% (239-268), 85.5% (224/262), 40.0% (18/45)]. There was significant (p<0.01) increase of embryo development rates in 5 IU-treated group compared with that in 20 and 40 IU-treated group [231/239(96.7), 179/224(79.9), 77.8(14/18)]. In conclusion, higher doses of PMSG injection increased the occurrence of abnormal oocytes ovulation in immature mice. The most of oocytes collected from 5 or 20 IU-PMSG-treated group has fertilizabioity. But in mice injected iwth higher doses of PMSG, their oocytes exhibit less fertilizability and, even fertilized, all oocytes are not fully capable of development.

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Suggestions for Integrating Foreign Language Teaching with Culture Education (외국어 교육과 문화 교육 통합을 위한 제언)

  • Kim, Yong-Seop
    • The Journal of the Korea Contents Association
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    • v.13 no.12
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    • pp.1069-1078
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    • 2013
  • The paper is about teaching culture in a language learning. There are many teachers who are afraid of teaching foreign language with culture. They are delighted to teach politics, economics, history, art and literature as objective culture. But they are hesitant to teach subjective culture which is connected with a communication competency. The paper suggests three stages to instruct culture in a schoolroom. The first stage, it is growing out of the self-centered view. The second step, be developing self-consciousness through comparing mother culture with target culture. The last stage, it has to response to appropriately target culture. The teaching materials for the model suggested are two movies. Because the cultural aspects in the moves are subjective culture, so it has something in common with target cultural situations in a language learning. This method which is teaching culture in a the language learning has the advantage of being easy to teachers and learners. Most students like to watch a movie. The teaching material is The Chronicles of Narnia : The Lion, the Witch and The Wardrobe와 Guess Who?. I hope that this suggestion for cultural teaching is helpful for understanding each other.

Real-time Detection of Deep Sleep using Electrodermal Activity (피부전기활동을 이용한 실시간 깊은 수면 검출 알고리즘의 개발)

  • Jung, Da Woon;Choi, Sang Ho;Joo, Kwang Min;Lee, Yu Jin;Jeong, Do-Un;Park, Kwang Suk
    • Journal of Biomedical Engineering Research
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    • v.36 no.5
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    • pp.204-210
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    • 2015
  • Although many studies have analyzed the relationship between electrodermal activity (EDA) and sleep stages, a practical method for detecting sleep stage using EDA has not been suggested. The aim of this study was to develop an algorithm for real-time automatic detection of deep sleep using the EDA signal. Simultaneously with overnight polysomnography (PSG), continuous measurement of skin conductance on the fingers was performed for ten subjects. The morphometric characteristics in the fluctuations of EDA signal were employed to establish the quantitative criteria for determining deep sleep. The 30-sec epoch-by-epoch comparison between the deep sleep detected by our method and that reported from PSG exhibited an average sensitivity of 74.6%, an average specificity of 98.0%, and an average accuracy of 96.1%. This study may address the growing need for a reliable and simple measure for identifying sleep stage without a PSG.

Effect of Grazing Stage and Intensity on the Forage Production and Nutritive Value in Orchargrass Dominant Pasture (Orchargrass 위주 혼파초지에서 방목시기와 강도가 초지생산성 및 사료가치에 미치는 영향)

  • Seo, Sung;Shin, Jae-Soon;Lee, Joung-Kyong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.1
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    • pp.53-60
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    • 1996
  • A field experiment was carried out to determine the effects of grazing stage and intensity on the forage production and nutritive value in orchardgrass dominant pasture, 1989 and 1990. The grazing stages were 20- 25m, 30-35cm and 40-45cm of plant height, and high and medium grazing intensity were set by the number of growing cows (initial body weight: 230-250kg), which was adJusted according to the pasture production. The high level of grazing intensity was 150% of medium intensity. Annual grazing frequency was 10 times in 20-25cm. 8 times in 30-35cm. and 6 times in 40-45cm of plant height. Dry matter(DM) yield was increased with increasing of plant height at grazing : 7,090kg in 20-25cm 7,882kg in 30-35n and 8,260 kgha in 40-45cm of height. Higher DM was observed at medium grazing intensity. In spring, daily DM production was more vigorous than those in summer and autumn season. Cmde protein (CP), digestible DM, and DM intake were decreased with increasing of plant height at grazing. CP content was 25.8% in 20-25cm 22.4% in 30-35m and 19.2% in 40-45cm, while the contents of neutral detergent fiber and acid detergent fiber were increased with higher plant height. Relative feed value was 11 1.2 in 20-25cm, 104.4 in 30-35n and 99.6 in 40-45cm. Also nutritive value of pre-grazing pasture plants was remarkably higher than that of post-grazing, and not significant differences of nutritive value were found between grazing intensity. From the above mults, it may be concluded that optimum plant height for grazing was 20-25cm and 30- 35cm in pasture mixtures dominated by orchardgms, and medium grazing intensity was very desirable for pasture productivity.

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Effects of Dietary Restriction on the Expression of Lipid Metabolism and Growth Hormone Signaling Genes in the Longissimus dorsi Muscle of Korean Cattle Steers

  • Kang, H.J.;Trang, N.H.;Baik, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.8
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    • pp.1187-1193
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    • 2015
  • This study determined the effects of dietary restriction on growth and the expression of lipid metabolism and growth hormone signaling genes in the longissimus dorsi muscle (LM) of Korean cattle. Thirty-one Korean cattle steers (average age 10.5 months) were allocated to normal (N; n = 16) or dietary restriction (DR; n = 15) groups. The feeding trial consisted of two stages: for the 8-month growing period, the DR group was fed 80% of the food intake of the normal diet, and for the 6-month growth-finishing period, the DR group was fed a DR total mixed ration with 78.4% of the crude protein and 64% of the net energy for gain of the normal diet. The LM was biopsied 5 months (period 1 [P1] at 15.5 months of age) and 14 months (period 2 [P2] at 24.5 months of age) after the start of feeding. The mRNA levels were determined using real-time polymerase chain reaction. Body weight, daily feed intake, average daily gain, and feed efficiency were lower in the DR group compared with the normal group at both P1 and P2. At P1, the lipogenic fatty acid synthase (FASN) mRNA levels were lower (p<0.05) in the DR group compared with the normal group. The DR group tended (p = 0.06) to have higher of levels of growth hormone receptor (GHR) mRNA than the normal group. At P2, the DR group tended to have lower (p = 0.06) androgen receptor (AR) mRNA levels than the normal group. In conclusion, our results demonstrate that dietary restriction partially decreases the transcription of lipogenic FASN and growth hormone signaling AR genes, but increases transcription of the GHR gene. These changes in gene transcription might affect body fat accumulation and the growth of the animals.

Analysis of Transgene Intergration Efficiency into Porcine Fetal Fibroblast using Different Transfection Methods

  • Kim, Baek-Chul;Kim, Hong-Rye;Kim, Myung-Yoon;Park, Chang-Sik;Jin, Dong-Il
    • Reproductive and Developmental Biology
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    • v.33 no.2
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    • pp.113-117
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    • 2009
  • Animals produced by somatic cell nuclear transfer (SCNT) using genetically modified cells are almost always transgenic, implying that this method is more efficient than the traditional pronuclear microinjection method. Most somatic cells for SCNT in animals are fetus-derived primary cells and successful gene integration in somatic cells will depend on transfection condition. The objective of this study is to evaluate the efficiency of electroporation (Microporator) and liposome reagents (F-6, F-HD, W-EX, W-Q, W-M) for tissue-type plasminogen activator (tPA) gene transfection and to estimate the overall efficiency of transfection of Korean native pig fetal fibroblast cells (KNPFF). Electroporation showed significantly higher transfection efficiency than liposome reagents with regard to the transfection of in vitro cultures in the early stages of development (41.7% with Microporator vs. 18.3% with F-6, 20.0% with F-HD 18.5% with W-EX, 5.0% with W-M and 6.3% W-Q,). Colonies identified as tPA-positives were treated once more with G418 for 10 to 14 days and growing colonies were selected again. When the cells of newly selected colonies were subjected to single-cell PCR, reselection of colonies following second round of G418 selection increased the rate of transgene integration per each colony. These results suggest that transfection with electroporation is the most efficient and the second rounds of G418 selection may be an effective method for transfection of porcine fetal fibroblast cells.

Anti-oxidative Capacity Analysis of Water-Soluble Substances According to Varieties and Maturity Stages in Mulberry Leaves and Fruits (뽕나무 품종별.시기별 뽕잎과 오디의 항산화능 분석)

  • Kim Hyun-Bok
    • Journal of Sericultural and Entomological Science
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    • v.47 no.2
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    • pp.62-67
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    • 2005
  • Much attention has been focused on the activity of the natural antioxidants present in fruits and vegetables, because potentially these components may reduce the level of oxidative stress. Especially, mulberry leaves containing many natural components are considerable resource far natural antioxidants. The antioxidant capacity of mulberry leaves was investigated with minilum L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The appropriate conditions for pre-treatments of mulberry leaves were 80% MeOH, 30 sec. vortex mixing. The antioxidant capacity of 16 varieties was 3303.4 nmol (ascorbic acid equivalents) at opening stage of five leaves in spring. The highest stage of antioxidant capacity (3708.0 nmol) and yield rate was just before the coloration stare with anthocyanin in fruits, whereas the lowest stage was middle of June (2231.6 nmol) and about two months growing stage after summer pruning (2064.6 nmol). But after summer pruning, the antioxidant capacity of mulberry.

A Pseudo-Random Number Generator based on Segmentation Technique (세그먼테이션 기법을 이용한 의사 난수 발생기)

  • Jeon, Min-Jung;Kim, Sang-Choon;Lee, Je-Hoon
    • Convergence Security Journal
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    • v.12 no.4
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    • pp.17-23
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    • 2012
  • Recently, the research for cryptographic algorithm, in particular, a stream cipher has been actively conducted for wireless devices as growing use of wireless devices such as smartphone and tablet. LFSR based random number generator is widely used in stream cipher since it has simple architecture and it operates very fast. However, the conventional multi-LFSR RNG (random number generator) suffers from its hardware complexity as well as very closed correlation between the numbers generated. A leap-ahead LFSR was presented to solve these problems. However, it has another disadvantage that the maximum period of the generated random numbers are significantly decreased according to the relationship between the number of the stages of the LFSR and the number of the output bits of the RNG. This paper presents new leap-ahead LFSR architecture to prevent this decrease in the maximum period by applying segmentation technique to the conventional leap-ahead LFSR. The proposed architecture is implemented using VHDL and it is simulated in FPGA using Xilinx ISE 10.1, with a device Virtex 4, XC4VLX15. From the simulation results, the proposed architecture has only 20% hardware complexity but it can increases the maximum period of the generated random numbers by 40% compared to the conventional Leap-ahead archtecture.

Approximation of Green Warranty Function by Radon Radial Basis Function Network (Radon RBF Network에 의해 그린 보증 함수의 근사화)

  • Lee, Sang-Hyun;Lim, Jong-Han;Moon, Kyung-Li
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.3
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    • pp.123-131
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    • 2012
  • As the price of traditional fuels soar, the alternatives are becoming more viable. And manufacturers are promoting the growing viability of electric and biofuel-powered vehicles through longer warranties. Now, these longer green environment (emission)warranties, sometimes called extended warranties or "super warranties," have been adapted. The main result of this paper is to present a new method to approximate a bivariate warranty function by using Radial Basis Function Network with application of Radon Transform and its inverse which is used to reduce the dimension of the warranty space. This method consist of the following stages: First, by using the Radon Transform, the bivariate warranty function can be reduced to one dimensional function. Second, each of the one dimensional functions is approximated by using neural network technique into neural sub-networks. Third, these neural sub-networks are combined together to form the final approximation neural network. Four, by using the inverse of radon transform to this final approximation neural network we get the approximation to the given function. Also, we apply the above method to some green warranty data of automotive vehicle company.