• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.568-576
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• 2014

TIM-1 (also known as KIM-1 and HAVcr-1) is a type I transmembrane glycoprotein member of the TIM family that may play important roles in innate and adaptive immune responses. The overexpression of proteins associated with membrane proteins is a major obstacle to overcome in studies of membrane protein structures and functions. In this study, we successfully coupled the overexpression of the TIM-1 protein with a C-terminal enhanced green fluorescent protein (GFP) tag in Escherichia coli. To the best of our knowledge, this report is the first to describe the overexpression of human TIM-1 in E. coli. The purified TIM-1-EGFP fusion protein recognized and bound directly to apoptotic cells and did not to bind to viable cells. Furthermore, we confirmed that the interactions of TIM-1-EGFP with apoptotic cells were blocked by TIM-1-Fc fusion proteins. This fusion protein represents a readily obtainable source of biologically active TIM-1 that may prove useful in future studies of human TIM-1.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.75-81
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• 2001

We have constructed a novel recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV) producing the green fluorescent polyhedra. For the production of the fluorescent polyhedra, partial polyhedrin gene containing KRKK as nuclear localization site from the BmNPV polyhedrin gene and the green fluorescent protein (gfp) gene were introduced under the control of p10 promoter of BmNPV. The recombinant BmNPV was stably produced fluorescent polyhedra in the infected Bm5 cells and the morphology of the fluorescent polyhedra was similar to that of wild-type BmNPV. The fluorescent polyhedra had 32 kDa native polyhedrin and 41 kDa fusion protein. From these data, we have further developed a novel BmNPV p10-based transfer vector producing recombinant polyhedra with foreign gene Product. The novel BmNPV P10-based transfer vector is composed of partial polyhedrin gene, factor Xa, and multiple cloning sites.

• Journal of Soil and Groundwater Environment
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• v.28 no.3
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• pp.1-11
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• 2023

Permeable reactive barrier (PRB) is a prominent in-situ remedial option for cleanup of contaminated groundwater and has been gaining increasing popularity in recent years. Funnel-and-gate systems, comprised of two side wings of impermeable walls and a central gate wall, are frequently implemented in many sites, but often suffers from bypassing of groundwater due to the progressive clogging of the gate wall over extended period of time. This study investigated technical feasibility of a hybrid funnel-and-gate system designed to address the flow deterioration in the gate wall. The key attribute of the proposed hybrid system is the operation of drainage units at the barrier walls and rear end of the gate wall. A conceptual modeling with MODFLOW indicated the groundwater inside the barrier was maintained at appropriate level to be guided toward the gate wall, yielding constant discharging of groundwater from the gate.

• Journal of Powder Materials
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• v.27 no.3
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• pp.256-267
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• 2020

Metal additive manufacturing (AM) technologies are classified into two groups according to the consolidation mechanisms and densification degrees of the as-built parts. Densified parts are obtained via a single-step process such as powder bed fusion, directed energy deposition, and sheet lamination AM technologies. Conversely, green bodies are consolidated with the aid of binder phases in multi-step processes such as binder jetting and material extrusion AM. Green-body part shapes are sustained by binder phases, which are removed for the debinding process. Chemical and/or thermal debinding processes are usually devised to enhance debinding kinetics. The pathways to final densification of the green parts are sintering and/or molten metal infiltration. With respect to innovation types, the multi-step metal AM process allows conventional powder metallurgy manufacturing to be innovated continuously. Eliminating cost/time-consuming molds, enlarged 3D design freedom, and wide material selectivity create opportunities for the industrial adoption of multi-step AM technologies. In addition, knowledge of powders and powder metallurgy fuel advances of multi-step AM technologies. In the present study, multi-step AM technologies are briefly introduced from the viewpoint of the entire manufacturing lifecycle.

• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1081-1087
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• 2011

An internally radiating photobioreactor was applied for the production of astaxanthin using the unicellular green alga Haematococcus pluvialis. The cellular morphology of H. pluvialis was significantly affected by the intensity of irradiance of the photobioreactor. Small green cells were widespread under lower light intensity, whereas big reddish cells were predominant under high light intensity. For these reasons, growth reflected by cell number or dry weight varied markedly with light conditions. Even under internal illumination of the photobioreactor, light penetration was significantly decreased as algal cells grew. Therefore, we employed a multistage process by gradually increasing the internal illuminations for astaxanthin production. Our results revealed that a multistage process might be essential to the successful operation of a photobioreactor for astaxnthin production using H. pluvialis.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.6
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• pp.1391-1396
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• 2011

A way to increase productivity in agriculture that is labor-centered industry is to graft IT technology. Today, many technologies in ubiquitous computing are deployed in all areas of society such as traffic control, automotive manufacturing, construction, defence, healthcare and clinical services. These IT technologies is gaining more attention as a fusion technology among traditional industries. To successfully build ubiquitous agriculture environment, it needs optimized core technology development for agriculture that includes sensor node H/W, middleware platform, routing protocol and agriculture environment application services. To achieve accurate botany growth environment management, we propose a green growth environment management system using environmental factor monitoring sensor and biological information sensors in greenhouse. By using our proposed system, it is expected to realize fusion complex agriculture technology with low cost.

• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.1
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• pp.35-38
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• 2007

In this study, ORF 46 of Bombyx mod nucleopolyhedrovirus(Bm46) fused with EGFP was expressed in Bombyx mod cell lines, larvae and pupae by BmNPV Bacmid system. Bm46 and EGFP were cloned into donor plasmid pFastBacHTb, which was transformed to competent DH10B cells containing helper and BmNPV bacmid by site-specific transposition. Recombinant bacmid was used to transfected BmN-4 cells to produce the recombinant baculovirus vBm-Bm46-EGFP. Recombination virus was injected into silkworm larvae and pupae. The expression of the fusion protein was monitored by examining green fluorescence using a fluorescent microscope. Intense fluorescence in cells and silkworm was observed at 4 days post-infection, indicating the Bm46-EGFP fusion gene was expressed successfully.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2001.11a
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• pp.142-142
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• 2001

• Journal of The Institute of Information and Telecommunication Facilities Engineering
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• v.9 no.3
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• pp.92-101
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• 2010

Energy efficiency and safe mobility are the two key constituents of the future automobile. The technologies that enable these features are now heavily dependent upon information and communication technology rather than traditional auto-mechanical technology. This paper presents an exploratory project 'Smart&Green Vehicle Project' at Western Michigan University which is to improve the geographical location accuracy of vehicles and to study various applications of making such location data available. Global Positioning System (GPS), Inertial Navigation System (INS), Vehicular Ad-hoc Network (VANET) technology, and data fusion among these technologies are investigated. Testing and evaluation is done on systems which will gather vehicular positioning data during GPS signal loss. Vehicles in urban settings do not acquire accurate positioning data from GPS alone; therefore there is a need for exploration into technology that can assist GPS in urban settings. The goal of this project is to improve the accuracy of positioning data during a loss of GPS signal. Controlled experiments are performed to gather data which aided in assessing the feasibility of these technologies for use in vehicular platforms.

• Clean Technology
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• v.11 no.2
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• pp.75-81
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• 2005

With a development of nanotechonlogy, the concept of environmental technology has been changed from end-of-pipe to green or clean technology. Nanoscience or nanotechnology involves studying and working with matter on a nano-scale. In this article, we introduced the necessity for the development of environmental sensing/monitoring technologies with nanotechnologies for the clean technology.