International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Rapid Expression of Bm46 in Bombyx mori Cell Lines, Larvae and Pupae

  • Wang, Haiyan (Institute of Life Sciences, Jiangsu University) ;
  • Chen, Keping (Institute of Life Sciences, Jiangsu University) ;
  • Guo, Zhongjian (Institute of Life Sciences, Jiangsu University) ;
  • Yao, Qin (Institute of Life Sciences, Jiangsu University) ;
  • Wang, Qiang (Institute of Life Sciences, Jiangsu University) ;
  • Mu, Runhong (College of medical technology, Jiangsu University)
  • Published : 2007.09.30
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Abstract

In this study, ORF 46 of Bombyx mod nucleopolyhedrovirus(Bm46) fused with EGFP was expressed in Bombyx mod cell lines, larvae and pupae by BmNPV Bacmid system. Bm46 and EGFP were cloned into donor plasmid pFastBacHTb, which was transformed to competent DH10B cells containing helper and BmNPV bacmid by site-specific transposition. Recombinant bacmid was used to transfected BmN-4 cells to produce the recombinant baculovirus vBm-Bm46-EGFP. Recombination virus was injected into silkworm larvae and pupae. The expression of the fusion protein was monitored by examining green fluorescence using a fluorescent microscope. Intense fluorescence in cells and silkworm was observed at 4 days post-infection, indicating the Bm46-EGFP fusion gene was expressed successfully.

Keywords

References

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