• Biomolecules & Therapeutics
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• v.28 no.6
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• pp.561-568
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• 2020

We examined the anticancer effects of a novel sirtuin inhibitor, MHY2256, on HCT116 human colorectal cancer cells to investigate its underlying molecular mechanisms. MHY2256 significantly suppressed the activity of sirtuin 1 and expression levels of sirtuin 1/2 and stimulated acetylation of forkhead box O1, which is a target protein of sirtuin 1. Treatment with MHY2256 inhibited the growth of the HCT116 (TP53 wild-type), HT-29 (TP53 mutant), and DLD-1 (TP53 mutant) human colorectal cancer cell lines. In addition, MHY2256 induced G0/G1 phase arrest of the cell cycle progression, which was accompanied by the reduction of cyclin D1 and cyclin E and the decrease of cyclin-dependent kinase 2, cyclin-dependent kinase 4, cyclin-dependent kinase 6, phosphorylated retinoblastoma protein, and E2F transcription factor 1. Apoptosis induction was shown by DNA fragmentation and increase in late apoptosis, which were detected using flow cytometric analysis. MHY2256 downregulated expression levels of procaspase-8, -9, and -3 and led to subsequent poly(ADP-ribose) polymerase cleavage. MHY2256-induced apoptosis was involved in the activation of caspase-8, -9, and -3 and was prevented by pretreatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, the autophagic effects of MHY2256 were observed as cytoplasmic vacuolation, green fluorescent protein-light-chain 3 punctate dots, accumulation of acidic vesicular organelles, and upregulated expression level of light-chain 3-II. Taken together, these results suggest that MHY2256 could be a potential novel sirtuin inhibitor for the chemoprevention or treatment of colorectal cancer or both.

• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.337-344
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• 2011

Integrase MJ1 from the bacteriophage ${\Phi}FC1$ carries out recombination between two DNA sequences (the phage attachment site, attP and the bacterial attachment site, attB) in NIH3T3 mouse cells. In this study, the integration vector containing attP, attB and the integrase gene MJ, was constructed. The integration mediated by integrase MJ1 in Escherichia coli led to excision of LacZ. Therefore, the frequency of integration was measured by the counting of the white colony, which is detectable on X-Gal plates. The extrachromosomal integration in NIH3T3 mouse cells was monitored by the expression of the green fluorescent protein (GFP) as a reporter. To demonstrate integration mediated integrase MJ1 in NIH3T3 cells, vectors containing attP and attB were co-transfected into NIH3T3 cells. The integration was confirmed by fluorescent microscopy. The expression of GFP was induced in NIH3T3 cells expressing MJ1 without accessory factors. By contrast, the excision mediated by the MJ1 between attR and attL had no effect on the expression of GFP. These results suggest that integrase MJ1 may enable a variety of genomic modifications for research and therapeutic purposes in higher living cells.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.8
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• pp.215-221
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• 2020

Navigation lights used in supersonic aircraft are used to identify the direction and location of the aircraft. The color of the navigation lights and location of installation are defined by aviation law as red for the left wing, green for the right wing, and white for the tail. Navigation lights operate in BRT and DIM modes. BRT is the brightest mode, and DIM is an output with dimmed brightness. Navigation lights serve to prevent aircraft collisions and are very important for stability and location identification. One phenomenon is that the inlet and tail navigation lights flicker abnormally. In this study, fault tree analysis was performed in two stages. The first step was derived from three causal factors, the second step developed five improvements, and the optimal improvement plan was drawn. The navigation lights confirmed that the initial input power was unstable as the main cause of abnormal flickering. As an improved method, the circuit was adjusted to stabilize the initial power, and it was confirmed that flickering did not occur as a result of the tests under the same conditions.

• Journal of Korean Society for Atmospheric Environment
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• v.26 no.1
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• pp.57-68
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• 2010

Increase in greenhouse gas emissions during the last century has led to remarkable changes in our environment and climate system. Many policy measures have been developed to reduce greenhouse gas emissions across the world, many of which require our lifestyle changes from energy-intensive to energy-saving. One of the changes in our living patterns is to consider food miles. A food mile is the distance food travels from where it is produced to where it is consumed. Providing information of food miles will help people choose low mileage food, helping promote a "green consumption" action and lead to a low carbon society with emission reduction systems. In this study, 10 items are selected from 23 Harmonized commodity description and 2-digit coding system (HS) to estimate their food miles, and $CO_2$ emissions released in the transportation of imported food. For the estimation, four countries are chosen-Korea, Japan, United Kingdom (UK) and France, with Korea and Japan's 2001, 2003, and 2007 trade statistics and UK and France's 2003 and 2007 trade statistics used. As a result, Korea showed in 2007 the highest level of food miles and $CO_2$ emissions per capita among 4 countries. That suggests that Korea should make an effort to purchase local food to reduce food miles and use low-carbon vehicles for food transport, contributing to reducing greenhouse gas emissions.

• Korean Journal of Medicinal Crop Science
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• v.25 no.1
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• pp.37-44
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• 2017

Background: The light emitting plasma (LEP) has recently attracted attention as a novel artificial light source for plant growth and functional component enhancement. We investigated the effects of LEP on whitening and antioxidant activities of the plant parts of perilla. Methods and Results: Previously germianted seeds of perilla were cultivated under different light conditions (fluoresce lamp, LED red, blue, white, green, and LEP) in a culture room for 2 months. Parts of perilla were harvested and extracted in 70% EtOH. The extracts were used to detect total phenolic contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power and tyrosinase inhibition activity as indicators of biological activity. Biological activity was highest in seedlings grown under LEP. The total phenolic content was highest in the stems and the total flavonoid content was highest in the roots of perilla exposed to LEP. The DPPH and ABTS radical activity in all the parts of perilla exposed to LEP were higher by approximately three-fold compared to that in the control (fluoresce lamp). The reducing power values of perilla significantly increased after treatment with LEP. In addition, all the extract of perilla plants exposed to LEP promoted the tyrosinase inhibitory activity. These results suggest that LEP can be an important artificial light source for enhancement of biological activity. Conclusions: LEP could promote whitening and antioxidant activity of perilla.

• Clean Technology
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• v.26 no.1
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• pp.1-6
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• 2020

Rapid expansion of supercritical solution (RESS) process was used to make molsidomine (MOL) loaded peracetyl-β-cyclodextrin (PAc-β-CD) nanoparticles, which were collected into the air. The effect of the concentration of the drug PAc-β-CD (0.5 and 1 wt%), extraction temperature (45 ~ 60 ℃), nozzle length (5 ~ 20 mm) and internal diameter (ID) (50 ~ 150 μm) of a capillary, and spray distance on the particle size and morphology of the resulting particles were investigated. The interaction of a drug and PAc-β-CD was confirmed by ¹H-NMR spectroscopy while the particle size was measured by means of a scanning electron microscope. It was found that increasing the temperature from 45 ℃ to 60 ℃ and decreasing the nozzle diameter from 150 μm to 50 μm had an increasing effect on the average particle size, while increasing the spray distance led to a decrease in the average particle size at a constant pressure of 34.5 MPa and temperature of 45 ℃. With 0.5 wt% of PAc-β-CD, the capillary nozzle of short length (5 mm) and small ID (50 μm) gave the smallest size (165 nm). The obtained nanoparticles showed increased dispersity and solubility in oil. The oil suspension of the inclusion complex showed increased sustainability, which can increase the in-vitro controlled release time of the drug.

• Land and Housing Review
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• v.9 no.2
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• pp.41-50
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• 2018

The purpose of this study is to review the U.S. renewable energy policies implemented by the federal government and the state governments to investigate potential barriers of renewable energy expansion and to develop policy implications for the successful renewable energy policy making in Korea. Recently, the restructuring in the energy supply chain has been being a new trend in many countries that shows a transition from traditional fossil fuels to sustainable renewable energy sources. The United States has enforced effective renewable energy policies (i.e., regulatory policies, financial incentives), which have led to the exploding growth of renewable energy facilities and productions over the last ten years. For example, many state governments in the U.S. are implementing Renewable Portfolio Standard (RPS) policies that require increased energy supply from renewable energy sources (i.e., solar, wind and geothermal). These RPS policies are expected to account for at least 10-50 percent of total electricity production in the next fifteen years. As part of results, in the recent three years, renewable energy in the U.S provided over 50 percent of total new power generation constructions. On the other hand, Korea initiated to develop climate change policies in 2008 for the Green Growth Policy that set up a target reduction of national Greenhouse Gas (GHG) emissions up to 37 percent by 2025. However, statistical data for accumulated renewable energy capacity refer that Korea is still in its early stage that contribute to only 7 percent of the total electricity production capacity and of which hydroelectric power occupied most of the production. Thus, new administration in Korea announced a new renewable energy policy (Renewable Energy 3020 Plan) in 2017 that will require over 95 percent of the total new generations as renewable energy facilities to achieve up to 20 percent of the total electricity production from renewable energy sources by 2030. However, to date, there have not been enough studies to figure out the barriers of the current policy environment and to develop implications about renewable energy policies to support the government plan in Korea. Therefore, this study reviewed the U.S. renewable energy policies compared with Korean policies that could show model cases to introduce related policies and to develop improved incentives to rapidly spread out renewable energy facilities in Korea.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.267-272
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• 2012

Vitex trifolia L. is a full grown fruit of Vitex rotundifolia L. (Verbenaceae). It has been used for treating headache, dizziness, toothache and removal of fever as a traditional medicine in Korea. V. trifolia (500 g) were extracted three times with 80% aqueous MeOH at room temperature. The MeOH extract (38 g) was successively partitioned with n-hexane, EtOAc, n-BuOH and $H_2O$. Both n-hexane and EtOAc fractions showed more than 80% antifungal activity in vivo against several plant pathogens at 2000 ppm. Successive repeated silica gel, Sephadex LH-20, preparative TLC, and preparative HPLC of these fractions led to isolation of three compounds. Using mainly mass spectroscopy and nuclear magnatic resonance (NMR) spectroscopy, their chemical structures were determined as agnuside(1), chrysosplenol B(2), and artemetin(3). Compound 2 was isolated for the first time from V. trifolia. Study on in vitro and in vivo antifungal activities of the isolated compounds is in progress.

• Ecology and Resilient Infrastructure
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• v.5 no.1
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• pp.35-44
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• 2018

Social, economic and environmental problems caused by rapid urbanization have been recently overcome by various civic participation projects. Local governance and resident - led partnership through field - based cooperative operating systems from urban regeneration to village projects are considered success factors. Among these, the village greening project which directly affects the residents and requires spontaneity requires the role and cooperation of the various participating actors due to the sharing of public space and private space. Social capital plays a key role in the sustainability and participation of the above - mentioned business as a relational capital centered on trust and participation, network and norms. Therefore, empirical research is needed. In this study, basic research was carried out to build a formation model of social capital in participation - type greening project expanding urban green space system to living area. We analyzed the elements of participation, the components of business progress, and the factors of social capital formation through literature review and in - depth interviews with participating experts. The purpose of this study is to provide basic data of social capital formation model for analyzing sustainability and activation strategies in the future.

• Journal of Environmental Impact Assessment
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• v.26 no.6
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• pp.479-494
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• 2017

After Fukushima incident, negative sentiment towards nuclear power has led to transition in policies that reduce the dependency on nuclear power in some countries. President Moon of Republic of Korea also announced a national plan of decommissioning retired nuclear power plants stage by stage. Therefore, nuclear power that once was considered the critical solution to energy security and climate change is now a limited option. This study aims to find an optimal energy mix in Korea's electricity system from 2016 through 2030 to combat climate change through energy transition with minimum cost. The study is divided into two different scenarios; energy transition and nuclear sustenance, to compare the total costs of the systems. Both scenarios show that electricity generated by wind technology increases from 2018 whereas that of photovoltaic(PV) increases from 2021. However, the total cost of the energy transition scenario was USD 4.7 billion more expensive than the nuclear sustenance scenario.