• 한국항공우주학회지
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• 제30권3호
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• pp.1-7
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• 2002

LEX를 가진 델타형 날개 모델을 사용한 풍동실험을 통하여 LEX가 델타형 날개 윗면의 표면압력분포에 미치는 영향에 대한 연구를 수행하였다. 풍동실험의 유속은 40m/sec, 전압과 전온도는 각각 101Pa, 278K이었으며, 단위길이당 레이놀즈 수는 $1.76{\times}10^6$이었다. LEX는 날개의 표면압력분포를 매우 많이 변화시키었다. LEX가 없는 경우에 비하여 표면압력의 피크치가 시위 앞부분에서는 감소하였으나 뒷부분으로 갈수록 그리고 받음각이 증가할수록 피크치도 증가하였다. 스팬방향 압력구배도 시위 앞부분에서는 완만하였으나 뒤로 갈수록 증가하였다. 또한 LEX가 있는 경우에는 모든 위치에서 받음각의 증가에 따라 표면압력 피크치도 거의 선형적으로 증가하였다.

• The Plant Pathology Journal
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• 제31권4호
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• pp.379-387
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• 2015

Melon necrotic spot virus (MNSV) was recently identified on watermelon (Citrullus vulgaris) in Korea, displaying as large necrotic spots and vein necrosis on the leaves and stems. The average occurrence of MNSV on watermelon was found to be 30-65% in Hapcheon and Andong City, respectively. Four isolates of the virus (MNSV-HW, MNSV-AW, MNSV-YW, and MNSV-SW) obtained from watermelon plants in different areas were non-pathogenic on ten general indicator plants, including Chenopodium quinoa, while they infected systemically six varieties of Cucurbitaceae. The virus particles purified by 10-40% sucrose density gradient centrifugation had a typical ultraviolet spectrum, with a minimum at 245 nm and a maximum at 260 nm. The morphology of the virus was spherical with a diameter of 28-30 nm. Virus particles were observed scattered throughout the cytoplasm of watermelon cells, but no crystals were detected. An ELISA was conducted using antiserum against MNSV-HW; the optimum concentrations of IgG and conjugated IgG for the assay were $1{\mu}l/ml$ and a 1:8,000-1:10,000 dilutions, respectively. Antiserum against MNSV-HW could capture specifically both MNSV-MN from melon and MNSV-HW from watermelon by IC/RT-PCR, and they were effectively detected with the same specific primer to produce product of 1,172 bp. The dsRNA of MNSV-HW had the same profile (4.5, 1.8, and 1.6 kb) as that of MNSV-MN from melon. The nucleotide sequence of the coat protein of MNSV-HW gave a different phylogenetic tree, having 17.2% difference in nucleotide sequence compared with MNSV isolates from melon.

• 미생물학회지
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• 제31권4호
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• pp.267-273
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• 1993

Escherichia coli 의 superoxide 라디칼 유도발현성 유전자의 탐색을 위하여 먼저 superoxide 라디칼에 의하여 발현이 유도되는 프로모터를 탐색하였다. 이를 위햐여, 프로모터 탐색벡터인 pJAC4 를 이용하여 E. coli MG1655 균주로부터 프로모터 library 를 구성하였다. Ampicillin 농도구 배정판법을 이용하여 프로모터 세기가 다른 클론들을 구별하여 프로모터 세기가 약한 것부터 중간정도까지 383개의 클론을 선별하였다. 이들 프로모터 클론들의 superoxide 라디칼 유도발현성을 paraquat 를 첨가한 ampicilin 농도구배평판에서 조사하였다. 이중 3개의 클론이 paraquat 에 의하여 유도됨을 확인하였고, 유도배율은 1.4-4배 정도이었다. 이들 프로모터의 염기서열을 결정한 결과 기종의 database 에 등록되어 있는 E. coli 염기서열들과는 다른 새로운 유전자임을 알았다. 이들 프로모터에 대하여 primer 연장법과 SS1 뉴클리아제 분석을 총하여 전사개시 자리를 결정하였고, paraquat 처리시 mRNA 의 양이 증가함을 관찰하였다. 특히 클론5의 경우는 두개의 전사개시 위치를 가지고 있으며, paraquat 처리시 상위 부분의 전사개시자리가 성택적으로 사용됨을 알 수 있었다. 프로모터 서열을 검색한 결과, RNA 중합효소($E\sigma^{70}$)에 의햐여 인지되는 -10과 -35부위를 가진 클론은 클론 5 뿐이었고, 나머지는 보존된 염기서열을 찾을 수 없었다. 이는 이들 프로모터들이 독특한 부류에 속하는 종류들로서, 새로운 전사조절인자를 요구할 가능성을 시사한다.

• 한국전자통신학회논문지
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• 제15권6호
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• pp.1201-1208
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• 2020

해양에서 바닷물의 성질이 비슷한 바닷물 덩어리를 수괴라 하며, 전선은 서로 다른 속성의 두 수괴가 만나는 해역이다. 경사도 알고리즘은 해수면 온도 픽셀이 급격하게 변하는 곳을 경사가 크다고 보고, 경사가 큰 곳을 전선으로 가정하여 추출하는 방법이다. 이 방법은 대용량의 위성 자료를 한꺼번에 처리할 수 있다는 장점이 있다. 따라서 본 연구에서는 경사도 알고리즘을 이용하여 한반도 주변 해역의 전선을 찾아보고자 하였다. 연구 자료는 격자화 되어있는 해수면 온도 위성 자료를 이용하였다 해상도는 1/4°이며, 연구 기간은 1993년 1월부터 2018년 12월까지 월 평균 자료를 사용하였다. 해수면 온도 자료를 이용하여 전선 추출 결과 대표적으로 중국 연안 전선, 남해 연안 전선, 쿠로시오/쿠로시오 속류 전선, 아극 전선, 아북극 전선 등 다섯 개의 전선을 찾을 수 있었다. 계절별 전선 분포 비교 결과 겨울, 봄철에는 여름, 가을철에 비해 더 많은 종류의 전선이 분포하였으며, 분포 범위도 더 넓어졌다.

• Food Science and Biotechnology
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• 제15권5호
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• pp.778-780
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• 2006

High performance liquid chromatography (HPLC) was used for the analysis of the lignans eleutheroside B and eleutheroside E in the fruits of Acanthopanax species. A reverse-phase system using a gradient of $H_2O$ and acetonitrile as the mobile phase was developed and detection was at 210 nm. The analysis was successfully carried out within 20 min. The content of eleutheroside B and eleutheroside E in Acanthopanax species was measured in the fruits of A. senticosus (0.58 and $1.66\;{\mu}g/mg$, respectively), A. sessiliflorus (1.15 and $8.49\;{\mu}g/mg$, respectively), A. koreanum (2.16 and $1.80\;{\mu}g/mg$, respectively), and A. divaricatus (1.06 and $7.08\;{\mu}g/mg$, respectively).

• Smart Structures and Systems
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• 제21권4호
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• pp.449-467
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• 2018

In this paper, a theoretical and numerical study on bridge simultaneous damage detection procedure for identifying both the system parameters and input excitation mass, are presented. This method is called 'Adjoint Variable Method' which is an iterative gradient-based model updating method based on the dynamic response sensitivity. The main advantage of proposed method is inclusion of an analytical method to augment the accuracy and speed of the solution. Moving mass is a model which takes into account the inertia effects of the vehicle. This interaction model is a time varying system and proposed method is capable of detecting damage in this variable system. Robustness of proposed method is illustrated by correctly detection of the location and extension of predetermined single, multiple and random damages in all ranges of speed and mass ratio of moving vehicle. A comparison study of common sensitivity and proposed method confirms its efficiency and performance improvement in sensitivity-based damage detection methods. Various sources of errors including the effects of measurement noise and initial assumption error in stability of method are also discussed.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제3권2호
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• pp.1-4
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• 1999

The Helmholtz equation is very important in physics and engineering. However, solution of the Helmholtz equation is in general known as a very difficult phenomenon. For if the ${\omega}$ is negative, the FDM discretized linear system becomes indefinite, whose solution by iterative method requires a very clever preconditioner. In this paper we assume that ${\omega}$ is nonnegative, and determine the optimal ${\rho}$ parameter for the three dimensional ADI iteration for the Helmholtz equation. The ADI(Alternating Direction Implicit) method is also getting new attentions due to the fact that it is very suitable to the vector/parallel computers, for example, as a preconditioner to the Krylov subspace methods. However, classical ADI was developed for two dimensions, and for three dimensions it is known that its convergence behaviour is quite different from that in two dimensions. So far, in three dimensions the so-called Douglas-Rachford form of ADI was developed. It is known to converge for a relatively wide range of ${\rho}$ values but its convergence is very slow. In this paper we determine the necessary conditions of the ${\rho}$ parameter for the convergence and optimal ${\rho}$ for the three dimensional ADI iteration of the Peaceman-Rachford form for the real Helmholtz equation with nonnegative ${\omega}$. Also, we conducted some experiments which is in close agreement with our theory. This straightforward extension of Peaceman-rachford ADI into three dimensions will be useful as an iterative solver itself or as a preconditioner to the the Krylov subspace methods, such as CG(Conjugate Gradient) method or GMRES(m).

• 한국약용작물학회지
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• 제14권5호
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• pp.289-292
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• 2006

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of $H_2O$ and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and $8.49\;{\mu}g/mg$, respectively), their fermented wine (0.45 and $1.33\;{\mu}g/mg$, respectively) and wine residues (no detection).

• Journal of the Korean Wood Science and Technology
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• 제19권4호
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• pp.7-17
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• 1991

The feasibility of treating three softwood(Japanese larch pitch pine, and Korean pine) thinned logs by double-diffusion treatment processes was investigated. Some posts were incised before immersion, and others were imersed in hot copper sulfate solution. Comparison among species indicated that. in general, pitch pine was most treatable and Japanese larch least treatable. For all three species, almost all treatment schedules gave consistently good penetration and high net retention, but very steep gradient of preservative distribution. As expected, the treatability was increased by the extension of immersion time, increased concentration of treating solution, incising. and heating of the first solution. Of the variables tested, it appears that heating of the first solution is the most important. From the data in this paper, it may be concluded that, if the first solution is not heated, the best schedule is #3. If the first solution is healed. it appears the best schedules are #10 or #11. Since heating of the first solution improves the treatability. schedules # 10 or :#11 are recommended if the cost of heating might be justified. The data presented in this paper indicate that double-diffusion treatment processes seem to offer a promise as a comparatively effective and easy-operating method of treating thinned logs for the small-scale production of treated stock.

• Bulletin of the Korean Chemical Society
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• 제9권1호
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• pp.1-4
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• 1988

Rates of solvolysis of trans-[Co$(N-eten)_2Cl_2$]$^+$ have been investigated using spectrophotometric method at various pressures and temperatures in the mixtures of water with the t-butyl alcohol which possesses a high structure inducing capacity in water. The values of ${\Delta}V^{\neq}$ obtained from pressure effect on the rate constants were 2.55∼ 5.83 $cm^3mol^{-1}$. These values were discussed in terms of dissociative mechanism. Extrema found in the variation of ${\Delta}H^{\neq}$ and ${\Delta}S^{\neq}$ with solvent composition correlated with extrema in the variation of the physical properties of the mixtures. The logarithms of rate constants correlated linearly with both Grunwald-Winstein parameter and the reciprocal of dielectric constant ($Ds^{-1}$). The gradient, m of Grunwald-Winstein plot for the trans-[Co$(N-eten)_2Cl_2$]$^+$ was 0.09, which is significantly lower than those for the other cobalt (Ⅲ)-dichloro complexes. It was suggested that the reaction is an Id mechanism with long extension of Co-Cl bond in the transition state, as found for the C-Cl bond in the transition state for the solvolysis of t-butyl chloride.