• Journal of the Korean Society of Food Science and Nutrition
• /
• v.35 no.8
• /
• pp.1010-1015
• /
• 2006

p-Pheylenediamimine (PPD) is one of hair dye's ingredients, and the mixture of PPD with hydrogen peroxide is generally used to dye hair at beauty shop. This study is conducted to investigate the effect of oxidized PPD on rat skin. 6% hydrogen peroxide, PPD (5% PPD in 2% $NH_4OH$) or the mixture (isovolumed mixture of 5% PPD and 6% hydrogen peroxide in 2% $NH_4OH$) was applied to rat skin ($25\;mg/16.5\;cm^2$) five times every other day. The activity of acid phosphatase (ACP) was more increased in the mixture of PPD with hydrogen peroxide applied group than PPD applied group. Furthermore, the activity of glucose 6-phosphatase (G6Pase) in the mixture of PPD with hydrogen peroxide applied group showed higher decreasing rate than that of PPD applied group. In histopathological findings, the mixed PPD with hydrogen peroxide applied group showed more thickening of epithelium, increased numbers of dermal fibroblasts, and the dilatation of dermal capillaries than PPD applied group. The significant increasing of xanthine oxidase (XO) activity was determined in mixture of PPD with hydrogen peroxide applied group compared with PPD applied group. However, reactive oxygen species (ROS) scavenging system, the activities of superoxide dismutase (SOD) and glutathione S-transferase (GST) were more significantly decreased in mixed PPD with hydrogen peroxide applied groups than in PPD applied group. In conclusion, topical application with the mixture of PPD with hydrogen peroxide compared with PPD application resulted in imbalance with ROS generating and scavenging which probably led to severe skin injury.

• Journal of Nutrition and Health
• /
• v.8 no.3
• /
• pp.9-17
• /
• 1975

The purpose of this survey was to study the nutritional status for over 40 years old, related to the dietary pattern of fishing area. The nutrition survey was conducted in a fishing area located in Chilpo-dong, Euchang-myon, Youngil-gun, Kyungbuk province. The precise weighing method was used in evaluating the foods intake for 40 households during a 7 days period. Physical examination, detailed biochemical test on both blood and urine and stool tests were performed by physians on 45 persons over 40 years old man. The result obtained are summarized as follows: (1) Average nutrients intake of an adult per day: calorie intake was 2,883 Cal and its components-Protein (76.3 g) was 10.6%, fat (13.7 g) was 4.2% and carbohydrate (521 g) contributed 85.2% of the total calories. Other nutrients were higher than any other survey data. (2) To evaluate the nutritional deficiencies, clinical examinations were conducted. Hepatomegaly was present 25.3% of these examiners. (3) By stool test most of the examiners were infested in ascareis and infestation rate of stool was 87.5%. (4) The following chemical components of blood serum were analyzed and found to be within the normal range: glucose, blood urea nitrogen, uric acid, total cholesterol, inorganic phosphate, alkaline phosphatase, and electrolytes. (5) Content of electrolytes, glucose, pH, specific gravity in urine were normal range.

• Korean Journal of Veterinary Research
• /
• v.30 no.1
• /
• pp.73-78
• /
• 1990

Hematological and blood chemical changes of rabbits infected with rabbit hemorrhagic disease virus were studied and the results were summarized as follows: 1. Total leukocyte count ($2,410{\pm}1,076/{\mu}l$), lymphocyte count ($1,582{\pm}632.5{\mu}l$) and heterophil count ($705{\pm}411.1{\mu}l$) were significantly decreased after 24 hours of the infection (p<0.01). However, no significant changes were observed in monocyte, eosinophil and basophil numbers. 2. A significant increase of aspartate aminotransferase (96IU/L), alanine aminotransferase (96IU/L) and alkaline phosphatase ($401.1{\pm}131.8IU/L$) was observed (p<0.01). 3. A moderate increase of BUN ($26.9{\pm}3.6mg/100ml$) and creatinine ($3.2{\pm}1.9mg/100ml$) was observed (p<0.05). 4. No significant changes of r-GTP, thymol turbidity, glucose, cholesterol, albumin, total serum protein, fibrinogen were observed.

• Biomedical Science Letters
• /
• v.10 no.3
• /
• pp.231-235
• /
• 2004

This study was investigated the effects of the lower power He-Ne IR laser treatment on the changes of blood biochemical components in the rat liver damaged by the carbon tetrachloride (CCl₄). The twenty one Sprague-Dawley adult male rats weights (260±18.6 g) were designed to the three groups: one control group and two experimental groups, the experimental groups were divided into the CCl₄-treated groups and the laser therapy group (CCl₄+ Laser). The experimental groups were injected twice with CCl₄(1.0 ml/kg body weight) intraperitoneal for two days. Each group was sacrificed after two weeks irradiated with the lower power He-Ne IR laser for ten minutes per every day. The activity of alanine aminotransferase (ALT), lactic dehydrogenase (LDH), alkaline phosphatase (ALP) and the concentration of serum glucose treated with He-Ne IR laser groups was significantly decreased to the conrtol (treated with carbon CCl₄) group. The activity of aspartate aminotransferase (AST) was decreased in the laser group but not significantly, the concentration of the serum cholesterol in the laser group was significantly increased comparing with the control and case control groups. In conclusion, the effect of the lower power He-Ne IR laser treatment is believed to be a possible protective effects for CCl₄ induced acute hepatotoxicity in rats.

• Nutrition Research and Practice
• /
• v.3 no.4
• /
• pp.279-285
• /
• 2009

This study was conducted to investigate the effect of dietary grape skin on lipid peroxidation and antioxidant defense system in rats fed high fat diet. The Sprague-Dawley rats were fed either control (5% fat) diet or high fat (25% fat) diet which was based on AIN-93 diet for 2 weeks, and then they were grouped as control group (C), control + 5% grape skin group (CS), high-fat group (HF), high fat + 5% grape skin group (HFS) with 10 rats each and fed corresponding diets for 4 weeks. The hepatic thiobarbituric acid reacting substances (TBARS) were increased in high fat group as compared with control group, but reduced by grape skin. The serum total antioxidant status, and activities of hepatic catalase and superoxide dismutase, xanthine oxidase and glucose-6-phosphatase were increased by supplementation of grape skin. Glutathione peroxidase activity was significantly higher in CS group than in C group. Grape skin feeding tended to increase the concentration of total glutathione, especially in control group. The ratio of reduced glutathione to oxidized glutathione was lower in high fat groups than in control groups. The ratio was increased by dietary supplementation of grape skin in control group. These results suggest that dietary supplementation of grape skin would be effective on protection of oxidative damage by lipid peroxidation through improvement of antioxidant defense system in rats fed high fat diet as well as rats with low fat diet.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.1
• /
• pp.30-34
• /
• 2001

The expediency of promoting rabbit production on underutilized by-product feedstuffs prompted the current investigation of caecal fermentation pattern, blood biochemical profile and histopathological changes of vital organs in 48 broiler rabbits fed diets containing 0, 5, 10 and 20% level of neem seed kernal cake (NSKC) for 6 weeks and subsequently sacrificed. The NSKC incorporation in the diet did not exert any adverse effect on caecal fermentation although the weight of caecum and its contents was significantly (p<0.01; p<0.05) lower in rabbits fed the diet containing 20% NSKC. Except for blood glucose concentration, none of the blood biochemical constituents (serum urea nitrogen, creatinine, total protein, haemoglobin) and the activities of different enzymes (alanine aminotransferase, asparatate aminotransferase, alkaline phosphatase) varied significantly due to NSKC feeding. A histopathological examination of the vital organs (liver, kidney, heart, spleen, lungs, intestine and stomach) revealed a variable degree of villus atrophy in the intestine and degenerative changes in the liver and tubular epithelium of kidney in some rabbits when NSKC was fed at levels above 10%.

• Korean Journal of Veterinary Service
• /
• v.23 no.1
• /
• pp.9-17
• /
• 2000

Epidemic malaria is prevalent in the demilitarized zone and the northern area of Kyonggi province in Korea. Malaria has broken out in Gangwha-Gun since 1997 due to geographical condition. The results obtained from an epidemiological surveillance for the malaria that happened in 1999 were as follows ; Anopheles sinensis collected in UV-light traps in the barn in Wolgogri and Dangsanri of Songhaemyon showed the distribution of 27,156 of 49,280 figures, corresponding to 55.1%, between July and August. The numbers of patients with malaria were 48(32.4%) in July, 36(24.3%) in August, and 38(25.7%) in September with a prevalence with the ages of the twenties to the seventies. Of a total 154 patients, the male were 86(58.1%) and the female were 68(41.8%). The annual parasite index(API) were 6.44, 5.89 and 4.45 in Songhaemyon, Yangsamyon and Kyodongmyon, respectively. A high infection rate was found Particularly in the areas within 2km from the Han river. By the immunofluorescence assays(IFA), 26 cases were positive with an antibody titers of $\geq$ 1 : 32, of 220 residents in Songhaemyon and 336 ones with a potential case and contacted with the patients. By the blood test, there was no significance in alkaline phosphatase, alanine aminotransferase, total cholesterol, glucose, hemoglobin, hematocrit, white blood cell, red blood cell, and platelet between residents with an antibody titers of $\geq$ 1 : 32 and < 1 : 32 in Songhaemyon.

• Journal of Environmental Health Sciences
• /
• v.19 no.2
• /
• pp.69-77
• /
• 1993

In the present study, the comparison of liver damage in carbon tetrachloride (CCl$_4$)-treated rats with that those pretreated with ethanol and an effect of liver injury on the serum and liver xanthine oxidase (XOD) activity were evaluated. The increasing rate of liver weight per body wt., the levels of serum alanine aminotransferase, and the decreasing rate of hepatic glucose-6-phosphatase activity and the protein contents in the liver cell were higher in carbon tetrachloride-treated animals pretreated with ethanol than the carbon tetrachloride-treated group. Especially, the histopathological findings also showed more severe liver damage in the ethanol-pretreated rats than the rats treated with carbon tetrachloride only. In such a experimental condition the xanthine oxidase activity of serum and liver both of carbon tetrachloride-treated rats and those pretreated with ethanol were higher than that of each control group. And the increasing rate of xanthine oxidase enzyme activity to the control group was higher in carbon tetrachloride-treated group pretreated with ethanol than those treated with CCl$_4$. In addition, the heptic uricase activity and the serum levels of uric acid were more increased in carbon tetrachloride-treated group pretreated with ethanol than those in the CCl$_4$-treated rats. On the other hand, there were no statistical differences in hepatic catalase and glutathione S-transferase activities between the CCl$_4$-treated rats and those pretreated with ethanol. In conclusion, it is assumed that the more severe liver damage in ethanol pretreated rats would be due to oxygen free radical produced by the xanthine oxidase system.

• Biomedical Science Letters
• /
• v.8 no.1
• /
• pp.47-52
• /
• 2002

To investigate an effect of cyclohexanone (CHO) treatment on the serum levels of xanthine oxidase (XO) in liver damaged animals, the rats were intraperitoneally pretreated with 50% carbon tetrachloride ($CCl_4$) in olive oil (0.1 mL/ 100 g body weight) 14 times every other day. To the $CCl_4$-pretreated rats, CHO (1.56 g/kg body weight) was injected once and then the animals were sacrificed at 4 hours after CHO treatment. The increasing rate of serum and liver XO activities to the control was higher in CHO-treated animals pretreated with $CCl_4$ than the $CCl_4$-pretreated those. Concomitantly CHO injection to the $CCl_4$-pretreated animals showed somewhat higher Vmax and lower Km value in the kinetics of liver XO enzyme. Furthermore, increasing rate of hepatic malonedialdehyde content to the control was also higher in CHO-treated animals pretreated with $CCl_4$ than $CCl_4$-pretreated those. On the other hand, the injection of CHO to the $CCl_4$-pretreated animals showed the more enhanced liver damage on the basis of liver function finding; liver weight per body weight (%), serum levels of alanine aminotransferase activity and hepatic glucose-6-phosphatase activity. In conclusion, injection of CHO to the $CCl_4$-pretreated rats led to more increased activity of serum XO and it may be caused by acceleration of hepatocyte membrane permeability and induction of enzyme protein.

• Korean Journal of Clinical Laboratory Science
• /
• v.44 no.2
• /
• pp.46-51
• /
• 2012

We analyzed the concentration of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Gamma Glutamyl Transferase (GGT), total protein (TP), albumin, total cholesterol (TC) and triglyceride in sera of patients (n=455) aged 60 years and older with hyperglycemia (${\geq}120mg/dL$ in serum). The average concentration of biochemicals in patient group was compared with those in reference group. Our data showed that the average concentrations of AST and TC in sera of senior patients with hyperglycemia were significantly increased by 27.5% (P<0.05) and 82% (P<0.05) in total group, 38.5% (P<0.05) and 75.0% (P<0.05) in sixty years' group, 16.0% (P<0.05) and 89.7% (P<0.05) in seventy years' group and 27.0% (P<0.05) and 79.5% (P<0.05) in eighty years' group, respectively. Patients with hyperglycemia showed a significant decrease in albumin level by 6.7% (total group, P<0.05), 4.5% (sixty years' group, P<0.05), 8.9% (seventy years' group, P<0.05) and 4.5% (eighty years' group, P<0.05), respectively. In conclusion, the high concentration of glucose in the senior patients with hyperglycemia may be a cause of severe liver function and lipid metabolism disorder.