• Journal of Plant Biotechnology
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• v.1 no.1
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• pp.1-7
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• 1999

Large-scale cultures of plant cell, tissue, and organ have been achieved by using BTBB. When different sized BTBBs (5 L, 20 L, 100 L, 300 L, and 500 L) were tested for the culture of yew cells (Taxus cuspidata Sieb. et Zucc.), cell growth increment reached to 94.5% in SCV after 24 days of culture with 30% of inoculation cell density. However, there were some variations in the production of taxol and its derivatives among the BTBBs of different size. Approximate 4 ㎎/l of taxol and 84 ㎎/l of total taxanes were obtained by using a 500L BTBB after 6 weeks of culture. With a 20L BTBB, about 20,000 cuttings of virus-free potatoes (cv. Dejima) could be obtained by inoculating 128 explants and maintaining 8 weeks under 16 hr light illumination. The frequency of ex vitro rooting of the cuttings revealed as more than 99% under 30% shade. By incorporating two-stage culture process consisting of multiple bulblet formation in solid medium and bulblet development in liquid medium, mass propagation of lily through bioreactor seemed to be possible. In the case of 'Marcopolo', the growth of mini-bulblets in BTBB was nearly 10 folds faster than that of the solid medium. Time course study revealed that maximum MAR yield of ginseng (Panax ginseng C. A. Meyer) in a 5 L and 20 L BTBB after 8 weeks of culture was 500 g and 2.2 ㎏, respectively. By cutting the MAR once and/or twice during the culture, the yield of root biomass could be increased more than 50% in fresh weight at the time of harvest. With initial inoculum of 500 g of sliced MAR in a 500 L BTBB, 74.8 ㎏ of adventitious root mass was obtained after 8 weeks of culture. The average content of total ginseng saponin obtained from small-scale and/or pilotscale BTBBs was approximately 1% per gram dry weight. Based on our results, we suggest that large-scale cultures of plant cell, tissue, and organ using BTBB system should be quite a feasible approach when compared with conventional method of tissue culture.

• Microbiology and Biotechnology Letters
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• v.38 no.2
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• pp.129-135
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• 2010

We described production of bioactive compounds from fungi grown on Korean ginseng-steaming effluents (GSE) for develop high-value added nutraceuticals from Korean GSE. Hansenula anomala KCCM 11473, which grew well in Korean GSE had high RNA content, and its optimal autolysis conditions were established to produce 5'-ribonucleotides (13.9~28.5 mg/g of biomass) at $55^{\circ}C$ and pH 5.0 for 24 h. 5'-Phosphodiesterase and adenyl deaminase were not effective in increasing the yield of 5'-ribinucleatides, but the yield of IMP increased significantly only after the addition of 1.0% adenyl deaminase. Saccharomyces cerevisiae showed the highest growth in the GSE medium. 267.1 mg of S. cerevisiae biomass was produced from 1 g of GSE solid and medicinal ginsenoside-$Rg_3$ contents was determined with 0.033 mg. Mucor miehei KCTC 6011 produced approximately 120 mg of chitosan per g-dry mycelium in 84 h at $25^{\circ}C$ when grown in the GSE (pH 8.0) supplemented with 0.5% yeast extract and 0.002% $CuSO_4$. Chitosan produced by M. miehei KCTC 6011 have deacetylated approximately 56% and its viscosity and molecular weight of the chitosan were 80 cps and $1.07\times10^3$ kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizotonia solani in 60 h.

• Korean Journal of Medicinal Crop Science
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• v.14 no.2
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• pp.113-116
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• 2006

This study was carried out to investigate spectral irradiance characteristics of blue, yellow, and blue-black colored polyethylene (PE) shading net and the effect on growth characteristics and yield in ginseng seedling. The spectral irradiance $({\mu}mol/m^2/s/nm)$ showed the peak at 498 nm in both of blue and blue-black PE shading net, and 606 nm under yellow PE one. The intensity of blue light in blue shading was more strong than that of blue-black shading, control. Blue shading was increased by 17% and 23% in accumulated quantum for daytime, $0.5^{\circ}C and $0.2^{\circ}C$ in maximum temperature on June 2 than that of yellow and blue-black shading, respectively, but heat injury ratio of the former was lower than that of the latter. Chlorophyll content and stem length in blue shading were decreased more significantly than those of yellow and blue-black shading. The specific leaf weight was higher under blue and yellow shading than that of blue-black shading. Ginseng seedling harvested in blue shading was increased by $13{\sim}17%$ in the number of root, and $17{\sim}20%$ in root weight per $m^ 2 compared to yellow and blue-black shading owing to the increase of survived plant, and the decrease of specific leaf weight, heat injury ratio, and stem length.

• Korean journal of applied entomology
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• v.10 no.1
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• pp.7-12
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• 1971

1. The number of Rhizoctonia solani, Pythium debaryanum, Fusarium and Trichoderma was detected by the modification of Boosails special plating method when ginseng seed bed was drenched with Captan, Difolatan, Zineb, Maneb and PCNB at weekly intervals. Pythium debaryanum Hesse was described for the first time on Panax ginseng in Korea. 2. The number of Rhizoctonia solani and Pythium debaryanum was decreased gradually as the geason Progressed, whereas that of Fusarium and rrichcderma was increased. 3, The number of Rhixoctonia solani was greatly reduced by PCNB, and soils treated with other fungicides generally showed less Rhizoctonie solani than in the control. The number of Pythium debaryanum was significantly reduced by Zineb, Maneb, followed by Captan and Difolatan. None of the fungicids reduced the number of Fusarium colonies in the fourth week. Effects of the chemicals on Trichoderma were not statistically significant. 4. More fresh weight of the seedling roots was obtained using Difolatan, Maneb and PCNB. Phytotoxicity was noted with Maneb, Zineb and Captan after the third treatment.

• Applied Biological Chemistry
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• v.40 no.2
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• pp.163-166
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• 1997

A yeast, Torulopsis bombicola, was cultured in the media fortified with soybean oil as a additional carbon source for 7 days with reciprocal shaking. From the culture broth, sophorose-lipid was isolated and treated with alkali to afford sophorose. The sophorose contained in the medium was acetylated and isolated through silica gel column chromatography. The aceylated sophorose was hydrolyzed with 5% KOH at room temperature to give rise to sophorose. Meanwhile, the MeOH extracts obtained from the pod of Sophora japonica was solvent-fractionated with n-BuOH and $H_2O$, and butanolic layer was chromatographed on silica gel column to afford a flavonoide-glycoside. The glycoside was hydrolyzed with 0.02 N $H_2SO_4$ to yield sophorose.

• Korean Journal of Food Science and Technology
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• v.21 no.6
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• pp.851-856
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• 1989

The physical properties of essential oil of nutmeg extracted in different methods (SDE, Solvent, $CO_2$) were investigated and also the components of essential oil were analyzed using G.C. and G.C./ Mass. Solvent extraction yield was 23% and was higher than those of SDE extraction and $CO_2$ extraction. From the analysis result and physical properties of nutmeg oil, it was conformed that the nutmeg used In this experiment was similar to East Indies type. The content of sabinene was 3.6-14.1%, 28.4-48.7% for myristicin, 1.2-2.1% for elemicin, and 3.6-5.6% for safrole. The camphene was identified in SDE extracts but not in $CO_2$ extracts. The volatile components of SDE extracts and solvent extracts contained large amount of monoterpene and terpene alcohol but $CO_2$ extracts aminly contained aromatic compounds.

• Journal of the Korean Applied Science and Technology
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• v.33 no.4
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• pp.751-761
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• 2016

In order to reduce the bitter taste of red ginseng extract(RGE), inclusion complexes(RGE-CD) of the extract with ${\alpha}$-, ${\beta}$-, ${\gamma}$-cyclodextine after acetic acid treatment at the steam process were prepared and studied for their taste by an electronic tongue. By complexation, the bitter taster- reducing efficacies of ${\alpha}$-CD and ${\beta}$-CD were much lower than that of ${\gamma}$-CD. This study suggested that by processing red ginseng with acetic acid it is possible to enhance the yield of both ginsenoside $Rg_3$ and nonpolar ginsenosides. Taste such as bitterness, sourness, saltiness, umami and sweetness of the red ginseng extract with different amounts of ${\alpha}$-CD, ${\beta}$-CD and ${\gamma}$-CD were checked using an electronic tongue. As a result, REG-${\gamma}$-CD10, prepared using 10%(w/w), showed significantly lower bitter taste than those of the other samples.

• Journal of Ginseng Research
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• v.34 no.3
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• pp.175-182
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• 2010

North American ginseng seedlings (Panax quinquefolius L.) were grown in pots in heated greenhouses, in a cool greenhouse, or in the field, in 11 experiments at various times over 16 years. Crop establishment, dry matter partitioning, photosynthesis, radiation use efficiency and carbon budget were measured and/or calculated in some years. Once the seedling canopy, of about $20\;cm^2$ per seedling, and a leaf area index of 0.37, was established, about 40 days after germination, full canopy display lasted about 87 days. Only 16.6% of the incoming solar radiation was intercepted by the crop, the remainder falling on the mulched soil surface. Total and root dry matter accumulations in the cool greenhouse and in the field were about double that in the heated greenhouses. Partitioning of dry matter to roots (economic yield or harvest index) in the cool greenhouse and in the field was 73% whereas it was 62.5% in the heated greenhouses. The relationship between root dry matter and radiation interception during the full canopy period was linear with growth efficiencies of $2.92\;mg\;MJ^{-1}$ at 4.8% of incoming radiation and $0.30\;mg\;MJ^{-1}$ at 68% of incoming radiation. A photosynthetic rate of $0.39\;g\;m^{-2}\;h^{-1}$ was attained at light saturation of about $150\;{\mu}mol\;m^{-2}\;s^{-1}$ (7.5% of full sunlight); dark respiration was $0.03\;g\;m^{-2}\;h^{-1}$, about 8.5% of maximum assimilation rate. Estimates of dry matter accumulation by growth analysis and by $CO_2$ uptake were similar, 6.21 vs. 7.62 mg $CO_2$, despite several assumptions in $CO_2$ uptake calculations.

• KSBB Journal
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• v.9 no.2
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• pp.157-164
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• 1994

Hairy root clones of Panax ginseng were established by selection of some hairy roots formed on the leaf, stem and root segments transformed with Agrobacterium rhizogenes strain $A_4$. The transformed roots grew well in MS medium under the dark condition. To confirm the transformation with Ri-T-DNA, dot blot hybridization and opine analysis were Performed. Among four hairy roots induced from different part of ginseng, the HB3 hairy roots were examined for selection of high-saponin-producing clones. Four clones isolated from HB3 hairy root cultures displayed various phenotypes characterized by growth and total saponin content. Maximum growth was obtained for cultures of HB3-10 clone and the content of total saponin was 0.55 wt%. However, higher amount of total saponin was obtained with HB3-2 clone cultures(0.74 wt%) in spite of lower growth. Dot blot hybridization confirmed the introduction of Ri-T-DNA in the plant genome. In the opine test, agropine and mannopine were detected from all hairy root clones.

• The Journal of Natural Sciences
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• v.4
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• pp.69-83
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• 1991

To determine optimum ethanol concentration and clarification time in ethanol clarification of red ginseng extract(RG-Ext), physical properties, crude saponin recovery of clarified RG-Ext. and stability of red ginseng drink prepared from clarified RG-Ext. were investigated. Color intensity, redness(a value), viscosity and yield of clarified RG-Ext. were decreased in proportion to the increase of ethanol concentration and clarification time, but transmittance, brightness(L value) and yellowness (b value) were decreased. Crude saponin recovery of clarified RG-Ext. were not change significantly by the increase of ethanol concentration. Red ginseng drink prepared from 50-90% ethanol clarified RG-Ext. were stable without precipitation until six months at the storage of $0-5^\circC$ and $40^\circC$.